##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043441_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3006450
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.54377022734454	31.0	31.0	33.0	30.0	34.0
2	30.566064295098872	31.0	31.0	34.0	26.0	34.0
3	31.53900314324203	31.0	31.0	34.0	28.0	34.0
4	35.16488815712884	37.0	35.0	37.0	32.0	37.0
5	35.49136589665552	37.0	35.0	37.0	33.0	37.0
6	35.76290675048645	37.0	35.0	37.0	35.0	37.0
7	33.671565134959835	37.0	35.0	37.0	32.0	37.0
8	34.71887308952419	37.0	35.0	37.0	31.0	37.0
9	36.81271300038251	39.0	37.0	39.0	32.0	39.0
10	36.79383458896705	39.0	35.0	39.0	32.0	39.0
11	36.553340650933826	39.0	35.0	39.0	33.0	39.0
12	36.55051439405278	39.0	35.0	39.0	33.0	39.0
13	36.5679223003875	39.0	35.0	39.0	33.0	39.0
14	37.73418217499044	40.0	36.0	41.0	33.0	41.0
15	37.79288296828486	40.0	36.0	41.0	33.0	41.0
16	37.579634785211795	40.0	36.0	41.0	32.0	41.0
17	37.52607793244524	40.0	36.0	41.0	32.0	41.0
18	37.34485256698099	39.0	35.0	41.0	32.0	41.0
19	37.143219078980195	38.0	35.0	41.0	32.0	41.0
20	36.812953483344145	38.0	35.0	41.0	32.0	41.0
21	36.7454476209483	38.0	35.0	40.0	32.0	41.0
22	36.708347719070666	38.0	35.0	40.0	32.0	41.0
23	36.639849656571705	38.0	35.0	40.0	32.0	41.0
24	36.50596983152888	38.0	35.0	40.0	32.0	41.0
25	36.38350845681784	37.0	35.0	40.0	31.0	41.0
26	36.11951770360392	37.0	35.0	40.0	31.0	41.0
27	35.99473598430042	37.0	35.0	40.0	31.0	41.0
28	35.832774534750286	37.0	35.0	40.0	30.0	41.0
29	35.673961649121054	37.0	35.0	40.0	30.0	41.0
30	35.504710871626	37.0	34.0	40.0	29.0	41.0
31	35.247157278517854	37.0	34.0	40.0	28.0	41.0
32	35.093167024231235	37.0	34.0	40.0	27.0	41.0
33	34.680440386502354	36.0	33.0	40.0	24.0	41.0
34	34.56204194315555	36.0	34.0	40.0	23.0	41.0
35	34.42880141030118	36.0	34.0	40.0	23.0	41.0
36	34.31506494370437	36.0	34.0	40.0	23.0	41.0
37	34.1778110395982	36.0	34.0	40.0	22.0	41.0
38	34.05206738844817	35.0	34.0	40.0	21.0	41.0
39	33.93690365713716	35.0	33.0	40.0	21.0	41.0
40	33.83676063130935	35.0	33.0	40.0	21.0	41.0
41	33.72291606379617	35.0	33.0	40.0	21.0	41.0
42	33.600480300686854	35.0	33.0	40.0	21.0	41.0
43	33.40588933792346	35.0	33.0	40.0	20.0	41.0
44	33.174932894277305	35.0	33.0	40.0	18.0	41.0
45	32.94162883134594	35.0	33.0	39.0	18.0	41.0
46	32.95868516023882	35.0	33.0	39.0	20.0	41.0
47	32.93114038151308	35.0	33.0	39.0	20.0	41.0
48	32.83930582580785	35.0	33.0	39.0	20.0	41.0
49	32.78308702955313	35.0	32.0	39.0	20.0	41.0
50	32.716043173842905	35.0	32.0	39.0	19.0	41.0
51	32.292842388863946	35.0	32.0	38.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	3.0
11	6.0
12	24.0
13	34.0
14	92.0
15	158.0
16	401.0
17	923.0
18	1781.0
19	3350.0
20	5465.0
21	8727.0
22	13659.0
23	20632.0
24	31767.0
25	47260.0
26	63069.0
27	74684.0
28	80680.0
29	83946.0
30	90424.0
31	102507.0
32	120899.0
33	157781.0
34	287686.0
35	307455.0
36	306875.0
37	317391.0
38	387892.0
39	490590.0
40	286.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.295830630810425	16.59099602521246	22.88772472517421	32.22544861880291
2	33.991351926690946	19.536962197941094	26.470488449832857	20.0011974255351
3	25.556886028372332	20.459611834555705	35.38838164612749	18.59512049094447
4	22.70049393803323	23.260656255716874	32.41756889354554	21.621280912704353
5	20.847477922466698	31.88045701741256	31.154717357680983	16.117347702439755
6	78.76329225498512	2.113223236707745	17.64526268522676	1.4782218230803772
7	74.4948693642003	7.465316236757638	16.421394002893777	1.618420396148281
8	73.48943105656173	3.4580318980857823	19.811272430940146	3.2412646144123465
9	44.4128789768664	17.193600425751303	24.944868532654795	13.448652064727504
10	29.424138103078377	16.288779124881504	32.6787074456585	21.608375326381612
11	26.733955329375174	13.800196244740476	40.387832826090566	19.078015599793776
12	22.28508706281495	15.690897902842222	40.709674200468996	21.31434083387384
13	18.331986229606343	18.493938033228556	43.80432071047248	19.369755026692612
14	19.482878477939096	17.371118761329807	43.41795140448036	19.728051356250727
15	24.75191671240167	14.977997305792545	40.98494902626021	19.285136955545575
16	23.47788920487618	16.30311496948228	38.30763857705932	21.911357248582213
17	23.808944103510786	19.271732441916544	38.0027274692744	18.916595985298276
18	23.611734770243974	24.00858154966821	33.98263732974105	18.397046350346756
19	25.939297177734538	20.752415639707962	33.41419281877297	19.89409436378453
20	25.67576377455138	22.93056594987444	35.26391591411798	16.129754361456204
21	22.258411082838563	18.635832959137854	37.68461141878295	21.421144539240633
22	24.899599195063946	19.12997056328893	36.00439056029536	19.96603968135176
23	25.65248050025778	18.53534899965075	36.26932095993614	19.542849540155334
24	26.378319945450613	16.361422940677546	33.861397994312235	23.398859119559614
25	19.94205790882935	18.327096742004688	36.7853448419232	24.945500507242762
26	18.214937883550366	19.100367543115635	37.86997954398044	24.814715029353557
27	19.62384207287665	22.015034342829583	36.616740674217105	21.744382910076666
28	24.150842355602123	22.197907831495616	33.771092151873475	19.88015766102879
29	22.32057742520248	19.436045834788537	38.74266992632507	19.500706813683912
30	27.977481747576043	19.051672238021585	34.03675431156347	18.934091702838895
31	28.34811821250977	20.451429426732524	32.25628232633172	18.944170034425984
32	27.77731211229191	20.044171697517005	31.418782949990852	20.759733240200237
33	21.955828302482992	21.71494619900547	35.81875634053452	20.510469157977017
34	21.88355036671157	25.21355751800296	32.67884049294018	20.22405162234529
35	24.759699978379818	28.10397645063114	28.99629130702323	18.140032263965807
36	25.788953749438708	25.469241131567134	28.19900547156946	20.542799647424705
37	23.522892447903672	29.819022435097875	27.645129637945086	19.012955479053367
38	24.22059239302167	24.735551896755307	28.121705000914698	22.92215070930832
39	22.510435896156597	22.147815529943955	32.429210530692345	22.912538043207103
40	20.158825192502785	23.57045685110346	32.94732990736583	23.323388049027923
41	20.092634169868116	26.107701774518123	28.596251392838727	25.203412662775033
42	19.367459961083668	20.927073458730398	30.884963994079396	28.82050258610654
43	20.600708476774933	20.42950988707612	34.10667065808512	24.86311097806383
44	24.731161336459945	22.07420712135575	31.881188777461787	21.313442764722513
45	23.462688552944503	23.824144755442465	31.84034326198673	20.8728234296263
46	21.085665818490245	23.610836701092648	31.703005205474895	23.60049227494221
47	20.18307305958855	24.73312378386469	33.86861580934325	21.21518734720351
48	23.273395532937517	23.752432270618172	32.17602155365963	20.79815064278468
49	20.84794358795257	21.70100949624973	34.402301717973025	23.048745197824676
50	19.716010577258896	21.974987111044587	36.63616557734205	21.672836734354473
51	19.442531889770326	20.246503351128407	34.536779257928785	25.774185501172482
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1516.0
1	16828.5
2	32141.0
3	122163.0
4	212185.0
5	127447.5
6	42710.0
7	37326.5
8	31943.0
9	31622.5
10	31302.0
11	30939.5
12	30577.0
13	29502.5
14	28428.0
15	26242.0
16	24056.0
17	22090.0
18	20124.0
19	18493.5
20	16863.0
21	16305.5
22	15748.0
23	15224.0
24	14700.0
25	15113.5
26	16613.5
27	17700.0
28	19281.0
29	20862.0
30	23309.5
31	25757.0
32	29304.0
33	32851.0
34	37847.0
35	42843.0
36	46909.5
37	50976.0
38	52877.5
39	54779.0
40	59816.5
41	64854.0
42	72799.5
43	80745.0
44	88771.5
45	96798.0
46	107917.5
47	119037.0
48	139520.5
49	160004.0
50	162499.5
51	164995.0
52	155286.5
53	145578.0
54	151024.0
55	156470.0
56	166587.5
57	176705.0
58	184900.5
59	193096.0
60	196810.5
61	200525.0
62	196263.0
63	192001.0
64	176407.0
65	160813.0
66	135130.5
67	109448.0
68	90512.0
69	71576.0
70	60675.5
71	49775.0
72	45987.5
73	42200.0
74	35157.0
75	21852.0
76	15590.0
77	11730.5
78	7871.0
79	5751.5
80	3632.0
81	2576.0
82	1520.0
83	1207.5
84	895.0
85	653.0
86	411.0
87	258.5
88	106.0
89	81.5
90	57.0
91	37.5
92	18.0
93	16.0
94	14.0
95	12.5
96	11.0
97	6.5
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3006450.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.089061685376752
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.67636730518265	20.50001782055321
2	8.414464841975853	4.558799143074356
3	2.91069420505496	2.3654392460400735
4	1.5973776784245968	1.7308584986275122
5	1.0700889765799109	1.4493853147707443
6	0.8387517927997927	1.3632599432324353
7	0.6987226566891057	1.3249418803615056
8	0.6272387477766561	1.3593047303984247
9	0.5521704925876378	1.346200248109625
>10	7.044401446140126	42.055292806663196
>50	0.4783489274763561	8.289357785479702
>100	0.0816149100716883	3.9103494190541195
>500	0.005984918467594516	1.1109495972132692
>1k	0.002992459233797253	1.5878814822895646
>5k	2.602138464171525E-4	0.5152781672934862
>10k+	5.20427692834305E-4	6.532683916838732
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	136223	4.5310249629962245	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	24961	0.8302482994894311	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13907	0.46257213657303464	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	10261	0.3412995393237872	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	8558	0.2846546591494953	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6062	0.2016331553825941	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	4623	0.1537693957990321	No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	4528	0.15060952285918608	No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	4241	0.14106338039880922	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	4185	0.13920071845532106	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.038849806249896056	0.0
2	0.0	0.0	0.0	0.1229024264497996	0.0
3	0.0	0.0	0.0	0.20569109747376474	0.0
4	0.0	0.0	0.0	0.2593091519898884	0.0
5	0.0	0.0	0.0	0.5735335694922583	0.0
6	0.0	0.0	0.0	0.7438008282193285	0.0
7	0.0	0.0	0.0	1.126045668479436	0.0
8	0.0	0.0	0.0	1.5919439870944136	0.0
9	0.0	0.0	0.0	2.0249131034941543	0.0
10	0.0	0.0	0.0	2.2844883500473983	0.0
11	0.0	0.0	0.0	2.5080410450863977	0.0
12	0.0	0.0	0.0	2.6727535798034228	0.0
13	0.0	0.0	0.0	2.770343760914035	0.0
14	0.0	0.0	0.0	2.8370337108549952	0.0
15	0.0	0.0	0.0	2.9849822880806265	0.0
16	0.0	0.0	0.0	3.143175505995443	0.0
17	0.0	0.0	0.0	3.375143441600559	0.0
18	0.0	0.0	0.0	3.5004407191205575	0.0
19	0.0	0.0	0.0	3.656638227810208	0.0
20	0.0	0.0	0.0	3.8224816644214936	0.0
21	0.0	0.0	0.0	4.018659881255301	0.0
22	0.0	0.0	0.0	4.192419631126412	0.0
23	0.0	0.0	0.0	4.362986246237257	0.0
24	6.652364083886311E-5	0.0	0.0	4.50032430274909	0.0
25	6.652364083886311E-5	0.0	0.0	4.640655923098671	0.0
26	6.652364083886311E-5	0.0	0.0	4.775166724874852	0.0
27	6.652364083886311E-5	0.0	0.0	4.935388913835254	0.0
28	6.652364083886311E-5	0.0	0.0	5.087927622278768	0.0
29	6.652364083886311E-5	0.0	0.0	5.252041444228243	0.0
30	6.652364083886311E-5	0.0	0.0	5.412396680470322	0.0
31	6.652364083886311E-5	0.0	0.0	5.590480466995959	0.0
32	6.652364083886311E-5	0.0	0.0	5.792080360558133	0.0
33	6.652364083886311E-5	0.0	0.0	6.002062232866005	0.0
34	6.652364083886311E-5	0.0	0.0	6.235726521312511	0.0
35	6.652364083886311E-5	0.0	0.0	6.470688020755376	0.0
36	6.652364083886311E-5	0.0	0.0	6.709374844085217	0.0
37	6.652364083886311E-5	0.0	0.0	7.0685359809742385	0.0
38	6.652364083886311E-5	0.0	0.0	7.425801194099353	0.0
39	6.652364083886311E-5	0.0	0.0	7.843602920387833	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	36205	0.0	44.086456	1
CGTTTTA	1770	0.0	43.347458	1
CACTTAC	8030	0.0	41.46949	36
CGCACTT	8070	0.0	41.180298	34
ATTACGG	235	0.0	41.17021	1
ACTTACT	8045	0.0	41.084526	37
GCACTTA	8130	0.0	40.931732	35
TCACGAC	490	0.0	40.867348	24
ACCCGCA	8165	0.0	40.59094	31
CGGTCTA	510	0.0	40.588234	30
CCGCACT	8190	0.0	40.57692	33
CTTACTG	8180	0.0	40.296455	38
CCCGCAC	8285	0.0	40.19312	32
GCGCGAC	2395	0.0	39.551147	8
TTATGAC	8580	0.0	39.519234	26
TATGACC	8565	0.0	39.50963	27
GCATAGG	445	0.0	39.4382	1
CATGCGG	515	0.0	39.320393	1
CTTATGA	8640	0.0	39.270836	25
AACGCAA	8815	0.0	39.2059	17
>>END_MODULE