##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043440_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3375748
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.51760083987312	31.0	31.0	33.0	30.0	34.0
2	30.538090224744263	31.0	31.0	34.0	26.0	34.0
3	31.52129779829537	31.0	31.0	34.0	28.0	34.0
4	35.13838799578642	37.0	35.0	37.0	32.0	37.0
5	35.45741388278983	37.0	35.0	37.0	33.0	37.0
6	35.752287493023765	37.0	35.0	37.0	35.0	37.0
7	33.65259018149459	37.0	35.0	37.0	31.0	37.0
8	34.681657813320186	37.0	35.0	37.0	31.0	37.0
9	36.72464858158843	39.0	37.0	39.0	32.0	39.0
10	36.68962167792146	39.0	35.0	39.0	32.0	39.0
11	36.30613689173481	38.0	35.0	39.0	32.0	39.0
12	36.265658751778865	39.0	35.0	39.0	32.0	39.0
13	36.23446773870561	39.0	35.0	39.0	32.0	39.0
14	37.39393995049394	40.0	35.0	41.0	32.0	41.0
15	37.45190428906423	40.0	35.0	41.0	33.0	41.0
16	37.19671640181672	40.0	35.0	41.0	31.0	41.0
17	37.13970622214691	39.0	35.0	41.0	31.0	41.0
18	37.02070385585654	39.0	35.0	41.0	31.0	41.0
19	36.89786411781922	38.0	35.0	41.0	32.0	41.0
20	36.66665787849093	38.0	35.0	40.0	32.0	41.0
21	36.58909225451663	38.0	35.0	40.0	31.0	41.0
22	36.549304035727786	38.0	35.0	40.0	31.0	41.0
23	36.49849988802482	38.0	35.0	40.0	32.0	41.0
24	36.38828313013886	37.0	35.0	40.0	31.0	41.0
25	36.27192106756784	37.0	35.0	40.0	31.0	41.0
26	35.98904420590637	37.0	35.0	40.0	31.0	41.0
27	35.87769377335038	37.0	35.0	40.0	30.0	41.0
28	35.728120404722155	36.0	35.0	40.0	30.0	41.0
29	35.54173149180567	36.0	34.0	40.0	30.0	41.0
30	35.36886817380918	36.0	34.0	40.0	29.0	41.0
31	35.191553694173855	36.0	34.0	40.0	28.0	41.0
32	35.10832088177198	36.0	34.0	40.0	27.0	41.0
33	34.771736219646726	36.0	33.0	40.0	25.0	41.0
34	34.65392677415494	36.0	34.0	40.0	24.0	41.0
35	34.56507861368799	36.0	34.0	40.0	23.0	41.0
36	34.52079805720095	36.0	34.0	40.0	23.0	41.0
37	34.4333276654537	36.0	34.0	40.0	23.0	41.0
38	34.337523417032315	36.0	34.0	40.0	23.0	41.0
39	34.24601717900744	35.0	34.0	40.0	23.0	41.0
40	34.16917139549516	35.0	34.0	40.0	23.0	41.0
41	34.08058095568745	35.0	33.0	40.0	23.0	41.0
42	33.9511103909415	35.0	33.0	40.0	23.0	41.0
43	33.79158248779233	35.0	33.0	40.0	22.0	41.0
44	33.57023273064222	35.0	33.0	40.0	21.0	41.0
45	33.330337750329704	35.0	33.0	40.0	20.0	41.0
46	33.34670597449809	35.0	33.0	40.0	20.0	41.0
47	33.331349377974895	35.0	33.0	39.0	20.0	41.0
48	33.26579916510356	35.0	33.0	39.0	20.0	41.0
49	33.22605271483535	35.0	33.0	39.0	20.0	41.0
50	33.172174285521315	35.0	33.0	39.0	20.0	41.0
51	32.75713767733848	35.0	32.0	39.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	4.0
10	5.0
11	12.0
12	28.0
13	38.0
14	91.0
15	235.0
16	481.0
17	1125.0
18	2161.0
19	4110.0
20	6755.0
21	10397.0
22	15578.0
23	23120.0
24	34169.0
25	49630.0
26	65061.0
27	76609.0
28	84468.0
29	91507.0
30	102684.0
31	118169.0
32	141289.0
33	186869.0
34	358823.0
35	351364.0
36	314219.0
37	337219.0
38	426159.0
39	573053.0
40	315.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.275881967492833	17.513629571875626	23.805420309809854	29.405068150821684
2	32.08829569031811	20.64517256619866	27.15176014323344	20.11477160024978
3	26.456417955368707	21.458355303772674	32.899997274678086	19.18522946618053
4	23.545744528323798	24.09679277007644	30.52526432660258	21.832198374997187
5	21.47775841087664	32.992480481362946	29.18230270742958	16.34745840033083
6	81.10765377036438	2.3212336939842664	15.092121805300632	1.4789907303507253
7	76.59049194430389	7.63528557226428	14.047582935693068	1.7266395477387528
8	75.53283005721991	3.6871828110392126	17.522546114224166	3.25744101751671
9	46.47358155881304	17.259700664860055	23.32632649119543	12.940391285131472
10	34.189193032181315	17.44081607987326	29.45418319139936	18.915807696546068
11	31.208964650204933	14.864142702595098	36.27439014997565	17.65250249722432
12	24.524312833777877	17.15027306540654	36.83924273968317	21.486171361132406
13	18.652725262667712	20.06331633759392	41.65829321382994	19.625665185908428
14	21.634257059472446	17.70247660666614	41.50872636227586	19.154539971585557
15	27.55513740954597	15.084686416166134	39.19163989729091	18.16853627699698
16	25.022424659660615	16.556982333989385	36.80262863223203	21.617964374117975
17	25.714330572068768	19.2369809594792	36.85008478120997	18.198603687242056
18	25.416500283788956	24.883610980440483	32.06196078617243	17.637927949598133
19	27.632305491997627	22.330458316201327	31.071661747263125	18.96557444453792
20	26.207169492509514	25.08504781755036	32.9224219343387	15.785360755601424
21	22.56530996982002	20.530857161138805	36.2244752866624	20.67935758237878
22	25.477671911528944	20.213890373333555	34.826770244698366	19.48166747043914
23	27.189529550191544	18.593953103134474	35.049417195833335	19.167100150840643
24	26.976421225755004	17.0425932267456	32.620325924802444	23.360659622696957
25	20.14039555085273	18.759072063436015	35.92593404483984	25.17459834087142
26	17.61452572881625	19.816111866170104	36.852217641838195	25.717144763175448
27	19.208661310026695	23.469020791836357	35.52489700060549	21.79742089753145
28	23.947374033843758	23.552883686815484	32.43251569726176	20.067226582078995
29	23.085431732463444	20.44500952085286	37.869681030693044	18.599877715990647
30	29.17015725107443	19.572017816495784	33.65162328467646	17.606201647753327
31	29.65751590462321	20.492139816123718	31.437773198710328	18.41257108054274
32	29.826545109409825	19.619444342409444	30.449755135750657	20.104255412430074
33	23.078588804614565	22.084409144284468	35.2110998806783	19.625902170422673
34	20.813609309699658	25.620010735398495	34.28425344545861	19.28212650944324
35	23.244329849266	29.52705592953028	29.437357290887828	17.79125693031589
36	24.5080201484234	27.70144572402916	28.079909993281486	19.71062413426595
37	23.617935935976263	31.009423689209033	26.951448982566234	18.421191392248474
38	24.37030252258166	25.69072098983692	27.077258136567067	22.861718351014353
39	22.653497832184154	22.28460181269455	31.105461663607592	23.956438691513704
40	19.630878845221858	24.369798930488887	31.540713347086335	24.45860887720292
41	19.365248827815346	26.22799450669896	27.88706384481306	26.519692820672635
42	18.412097111514246	21.13300518877594	30.44279371564465	30.012103984065163
43	19.764597357385682	20.095635100724344	32.72498421090674	27.41478333098324
44	23.575752692440314	22.3897933139559	30.72507189517701	23.309382098426777
45	23.231443816303823	24.72995614601564	31.02171726088559	21.01688277679495
46	20.628909503908467	24.185395355340507	30.586435954342562	24.599259186408464
47	18.97887520040003	24.42951902807911	34.26141406289806	22.330191708622802
48	22.92052013361187	22.83967879118939	32.85340019456429	21.386400880634454
49	20.655185161925594	21.551549464000274	34.19736899792284	23.595896376151302
50	18.73487001991855	22.189482153288694	36.88829853413228	22.18734929266047
51	18.00620188473784	19.67341756552918	35.51912050307073	26.80126004666225
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1669.0
1	16937.5
2	32206.0
3	115983.5
4	199761.0
5	119875.0
6	39989.0
7	34216.5
8	28444.0
9	28175.5
10	27907.0
11	27766.5
12	27626.0
13	26359.5
14	25093.0
15	23505.5
16	21918.0
17	20510.0
18	19102.0
19	18176.0
20	17250.0
21	17277.0
22	17304.0
23	17147.5
24	16991.0
25	17508.0
26	20112.0
27	22199.0
28	24418.0
29	26637.0
30	31074.5
31	35512.0
32	38940.0
33	42368.0
34	48478.5
35	54589.0
36	59190.5
37	63792.0
38	68551.0
39	73310.0
40	82417.0
41	91524.0
42	102376.5
43	113229.0
44	125151.0
45	137073.0
46	147616.0
47	158159.0
48	175586.0
49	193013.0
50	186992.0
51	180971.0
52	167232.5
53	153494.0
54	160276.5
55	167059.0
56	177528.0
57	187997.0
58	198467.0
59	208937.0
60	219361.5
61	229786.0
62	227840.5
63	225895.0
64	206784.0
65	187673.0
66	151921.5
67	116170.0
68	91641.5
69	67113.0
70	56903.5
71	46694.0
72	43673.0
73	40652.0
74	34766.0
75	21844.0
76	14808.0
77	11162.5
78	7517.0
79	5779.5
80	4042.0
81	2891.0
82	1740.0
83	1329.5
84	919.0
85	669.5
86	420.0
87	291.5
88	163.0
89	115.0
90	67.0
91	51.0
92	35.0
93	25.5
94	16.0
95	11.5
96	7.0
97	4.5
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3375748.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.69464421408018
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.2314680617723	19.072036259165635
2	8.903801245730282	4.3975240783238645
3	2.9321457016699495	2.172248846597517
4	1.4565446246788711	1.4387540515350272
5	0.891488191552676	1.1007491855723548
6	0.677969586482229	1.0045330635687426
7	0.5052061387401382	0.873312009566986
8	0.41296427875203745	0.8158404749524638
9	0.3524970604305007	0.7834310544456303
>10	5.162143362488521	30.839437832995483
>50	1.2318900739133645	20.730356922675057
>100	0.2328798265868834	8.679165893561997
>500	0.005401108321379997	0.9156748047523713
>1k	0.00282915197786571	1.3520446282975715
>5k	3.8579345152714233E-4	0.6996652319589453
>10k+	3.8579345152714233E-4	5.125225662030418
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	123121	3.647221297324326	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	24957	0.739302815257537	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13312	0.3943422317068691	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	9322	0.2761462052262195	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	7186	0.21287133992229276	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5524	0.16363780708749587	No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	4823	0.1428720390266098	No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	4355	0.1290084449431652	No Hit
GGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAG	3424	0.10142937209768028	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03892470646505604	0.0
2	0.0	0.0	0.0	0.12444649304391205	0.0
3	0.0	0.0	0.0	0.19986681470299322	0.0
4	0.0	0.0	0.0	0.24856713238073458	0.0
5	0.0	0.0	0.0	0.5380140934690623	0.0
6	0.0	0.0	0.0	0.6534847980358723	0.0
7	0.0	0.0	0.0	0.9540404082295243	0.0
8	0.0	0.0	0.0	1.3238843657761183	0.0
9	0.0	0.0	0.0	1.6617650369636596	0.0
10	0.0	0.0	0.0	1.8814200586062704	0.0
11	0.0	0.0	0.0	2.0785023052668623	0.001925499178256197
12	0.0	0.0	0.0	2.2327199779130433	0.001925499178256197
13	0.0	0.0	0.0	2.320522740441526	0.001925499178256197
14	0.0	0.0	0.0	2.383945721066857	0.001925499178256197
15	0.0	0.0	0.0	2.548620335404183	0.001925499178256197
16	0.0	0.0	0.0	2.7313057728242747	0.001925499178256197
17	0.0	0.0	0.0	2.967283102885642	0.001925499178256197
18	0.0	0.0	0.0	3.1126434793118443	0.001925499178256197
19	0.0	0.0	0.0	3.276073924949374	0.001925499178256197
20	0.0	0.0	0.0	3.4650098289327285	0.001925499178256197
21	0.0	0.0	0.0	3.6548344248445086	0.001925499178256197
22	0.0	0.0	0.0	3.838467800321588	0.001925499178256197
23	0.0	0.0	0.0	4.019583215334794	0.001925499178256197
24	2.9623064280864568E-5	0.0	0.0	4.177089048116151	0.001925499178256197
25	2.9623064280864568E-5	0.0	0.0	4.332491643333566	0.001925499178256197
26	2.9623064280864568E-5	0.0	0.0	4.488397830643756	0.001925499178256197
27	2.9623064280864568E-5	0.0	0.0	4.658937811708694	0.001925499178256197
28	2.9623064280864568E-5	0.0	0.0	4.830336861637776	0.001925499178256197
29	2.9623064280864568E-5	0.0	0.0	5.016606689835853	0.001925499178256197
30	2.9623064280864568E-5	0.0	0.0	5.194552436971006	0.001925499178256197
31	2.9623064280864568E-5	0.0	0.0	5.395396812795268	0.001925499178256197
32	2.9623064280864568E-5	0.0	0.0	5.621717023901073	0.001925499178256197
33	2.9623064280864568E-5	0.0	0.0	5.835802909458882	0.001925499178256197
34	2.9623064280864568E-5	0.0	0.0	6.090739000660002	0.001925499178256197
35	2.9623064280864568E-5	0.0	0.0	6.315044843394708	0.001925499178256197
36	2.9623064280864568E-5	0.0	0.0	6.5631972528755105	0.001925499178256197
37	2.9623064280864568E-5	0.0	0.0	6.927472074337302	0.001925499178256197
38	2.9623064280864568E-5	0.0	0.0	7.2867998440641895	0.001925499178256197
39	2.9623064280864568E-5	0.0	0.0	7.772381113756121	0.001925499178256197
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	32900	0.0	43.92629	1
CGCACTT	7720	0.0	41.15285	34
ACCCGCA	7730	0.0	41.128723	31
CCGCACT	7770	0.0	41.00386	33
CCCGCAC	7800	0.0	40.846153	32
GCACTTA	7850	0.0	40.528664	35
CACTTAC	7880	0.0	40.460022	36
CTTACTG	7880	0.0	40.26015	38
ACTTACT	7920	0.0	40.198864	37
TTATGAC	8120	0.0	39.846058	26
TGACCCG	7980	0.0	39.75564	29
TATGACC	8140	0.0	39.637592	27
CTTATGA	8180	0.0	39.608803	25
CAACGCA	8305	0.0	39.55448	16
ATCAACG	8300	0.0	39.524097	14
TCAACGC	8325	0.0	39.48649	15
TATGCGG	765	0.0	39.411762	1
TAGCATA	2080	0.0	39.266827	29
ACGGGAT	745	0.0	39.261745	4
AACGCAA	8375	0.0	39.250748	17
>>END_MODULE