##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043439_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	755370
Sequences flagged as poor quality	0
Sequence length	51
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.48719038352066	31.0	31.0	33.0	30.0	34.0
2	30.518430702834372	31.0	31.0	34.0	26.0	34.0
3	31.422917245853025	31.0	31.0	34.0	28.0	34.0
4	35.04777261474509	37.0	35.0	37.0	32.0	37.0
5	35.37272992043634	37.0	35.0	37.0	33.0	37.0
6	35.79116724254339	37.0	35.0	37.0	35.0	37.0
7	33.708289977097316	37.0	35.0	37.0	32.0	37.0
8	34.73504242953784	37.0	35.0	37.0	31.0	37.0
9	36.718610747051116	39.0	37.0	39.0	32.0	39.0
10	36.676492315024426	39.0	35.0	39.0	32.0	39.0
11	36.40026741861604	38.0	35.0	39.0	32.0	39.0
12	36.29306300223731	39.0	35.0	39.0	32.0	39.0
13	36.368938401048496	39.0	35.0	39.0	32.0	39.0
14	37.71130968929134	40.0	36.0	41.0	33.0	41.0
15	37.88083720560785	40.0	36.0	41.0	33.0	41.0
16	37.85685822841786	40.0	36.0	41.0	33.0	41.0
17	37.792840594675454	40.0	36.0	41.0	33.0	41.0
18	37.51820697141798	39.0	36.0	41.0	33.0	41.0
19	37.21669115797556	38.0	35.0	41.0	33.0	41.0
20	36.8892992837947	38.0	35.0	41.0	32.0	41.0
21	36.78965010524644	38.0	35.0	40.0	32.0	41.0
22	36.692530812714296	38.0	35.0	40.0	32.0	41.0
23	36.594500708262174	37.0	35.0	40.0	32.0	41.0
24	36.44646067490104	37.0	35.0	40.0	32.0	41.0
25	36.329232031984326	37.0	35.0	40.0	32.0	41.0
26	36.0549876219601	36.0	35.0	40.0	31.0	41.0
27	35.95329970742815	36.0	35.0	40.0	31.0	41.0
28	35.86293604458742	36.0	35.0	40.0	31.0	41.0
29	35.761573798271044	36.0	35.0	40.0	31.0	41.0
30	35.574247057733295	36.0	35.0	40.0	30.0	41.0
31	35.11665144233952	35.0	34.0	40.0	28.0	41.0
32	34.905275560321435	35.0	34.0	40.0	26.0	41.0
33	34.77622489640838	35.0	34.0	40.0	25.0	41.0
34	34.573352132067726	35.0	34.0	40.0	24.0	41.0
35	34.33711558573944	35.0	34.0	40.0	23.0	41.0
36	34.174114672279806	35.0	34.0	40.0	22.0	41.0
37	34.09855302699339	35.0	33.0	40.0	22.0	41.0
38	34.02848934959027	35.0	33.0	40.0	21.0	41.0
39	33.91048757562519	35.0	33.0	40.0	21.0	41.0
40	33.84323179369051	35.0	33.0	40.0	21.0	41.0
41	33.780330169321	35.0	33.0	40.0	21.0	41.0
42	33.66592398427261	35.0	33.0	40.0	20.0	41.0
43	33.51390444417967	35.0	33.0	40.0	19.0	41.0
44	33.3072904669235	35.0	33.0	40.0	18.0	41.0
45	33.14692931940638	35.0	33.0	40.0	18.0	41.0
46	33.14120894396124	35.0	33.0	40.0	18.0	41.0
47	33.133738432821005	35.0	33.0	40.0	18.0	41.0
48	33.07751962614348	35.0	33.0	40.0	18.0	41.0
49	32.98552629837034	35.0	33.0	39.0	18.0	41.0
50	32.86590412645459	35.0	33.0	39.0	18.0	41.0
51	32.49022465811456	35.0	32.0	39.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	6.0
13	7.0
14	14.0
15	34.0
16	83.0
17	209.0
18	451.0
19	839.0
20	1389.0
21	2221.0
22	3536.0
23	5663.0
24	8551.0
25	13051.0
26	16514.0
27	18094.0
28	18091.0
29	18736.0
30	20523.0
31	24511.0
32	29879.0
33	39160.0
34	65717.0
35	94476.0
36	87476.0
37	68734.0
38	85791.0
39	131542.0
40	71.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.655996399115665	19.18940386830295	25.214265856467694	30.94033387611369
2	32.17231290625786	20.67556296914095	28.811178627692392	18.3409454969088
3	21.32676701484041	21.094562929425315	39.88098547731575	17.697684578418524
4	21.51568105696546	24.46827382607199	36.45140791929783	17.56463719766472
5	19.037292982247113	30.67437150005957	34.06833737108966	16.21999814660365
6	77.35454148298186	1.9942544713186916	19.10600103260654	1.5452030130929213
7	73.71989885751353	7.411731998887962	17.23234970941393	1.6360194341845717
8	72.83318109006183	4.151475435879106	20.375047989726887	2.6402954843321815
9	38.853806743715005	23.853740550988256	26.3963355706475	10.896117134649245
10	28.972159339131814	24.91189748070482	35.69456028171625	10.421382898447117
11	27.270344334564516	22.15377894276977	37.5454413069092	13.030435415756516
12	19.067874022002464	30.239485285356842	36.52607331506414	14.166567377576552
13	13.929994572196408	33.4715437467731	36.37899307624078	16.219468604789704
14	12.527238307054823	26.144803209023394	44.89018626633305	16.43777221758873
15	14.232098177052304	24.20310576273879	44.51566781842011	17.049128241788793
16	15.827475277016562	25.126229529899252	35.955756781444855	23.09053841163933
17	16.68334723380595	25.329441201000837	40.58938003892133	17.397831526271894
18	20.83508744059203	27.408025206190345	35.46354766538253	16.2933396878351
19	21.02611964997286	26.89966506480268	35.59368256615963	16.48053271906483
20	16.115943180163363	30.561049551875243	37.07560533248607	16.24740193547533
21	17.890305413241194	27.538822034234876	34.638653904708946	19.932218647814977
22	13.89160279068536	30.498034076015728	35.23478560175808	20.375577531540834
23	12.855289460794047	27.16748083720561	44.575506043395954	15.401723658604393
24	14.494486145862293	27.72654460727855	41.32636985847995	16.452599388379205
25	13.69130359956048	32.643737506122825	36.35291314190397	17.312045752412725
26	13.592279280352676	33.079285648092984	36.39514410156612	16.933290969988217
27	13.530322888121052	31.26428108079484	40.08816871202192	15.117227319062183
28	13.00912135774521	28.14316162940016	39.07952394190926	19.768193070945365
29	14.443782517177011	32.89076876232839	37.05508558719568	15.610363133298913
30	19.055826945735202	30.66761984193177	34.269430874935466	16.00712233739757
31	18.51860677548751	30.706541165256763	34.696903504242954	16.077948555012775
32	16.882719726756424	33.02103604855898	33.24278168314866	16.853462541535936
33	14.144856163204786	31.818578974542277	34.45781537524657	19.578749487006366
34	14.396653295735865	30.511669777724826	35.48380263976594	19.60787428677337
35	19.664402875412048	30.9392747924858	32.954975707269284	16.441346624832864
36	15.01767345804043	33.36748878033281	34.281477951202724	17.33335981042403
37	14.871652302844963	32.88441426056105	33.96772442644002	18.276209010153966
38	14.769847889113944	33.748891271827056	33.259991792101886	18.22126904695712
39	18.141175847597864	30.82145173888293	32.17151859353694	18.86585381998226
40	13.852681467360368	29.118048108873793	34.84199796126401	22.18727246250182
41	15.460238029045367	30.731032474151743	31.95784847160994	21.850881025192955
42	15.414035505778626	30.760024888465253	32.96265406357149	20.863285542184627
43	15.254775805234521	31.68195718654434	34.26982803129592	18.793438976925213
44	12.908773184002541	36.79812542197863	31.459152468326778	18.833948925692045
45	16.310417411334843	33.59969286574791	32.01742192567881	18.07246779723844
46	14.761772376451276	32.44621840952116	31.836318625309453	20.955690588718113
47	14.347008750678475	31.586110118220212	35.00880363265684	19.05807749844447
48	15.648490143902988	29.802083747037877	34.61853131577902	19.930894793280114
49	15.929544461654555	31.905688602936312	32.89566702410739	19.269099911301744
50	13.872804056290294	31.324384076677653	33.59730992758516	21.205501939446894
51	14.335623601678648	29.835974423130384	34.310073209155775	21.51832876603519
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	993.0
1	5043.5
2	9094.0
3	34439.0
4	59784.0
5	35043.5
6	10303.0
7	10436.0
8	10569.0
9	12463.5
10	14358.0
11	16145.0
12	17932.0
13	17509.5
14	17087.0
15	15341.0
16	13595.0
17	11800.5
18	10006.0
19	8927.0
20	7848.0
21	7243.0
22	6638.0
23	6266.5
24	5895.0
25	6438.0
26	6849.5
27	6718.0
28	8094.5
29	9471.0
30	10670.5
31	11870.0
32	13816.0
33	15762.0
34	17270.0
35	18778.0
36	21164.5
37	23551.0
38	25692.5
39	27834.0
40	31454.5
41	35075.0
42	41677.5
43	48280.0
44	52307.0
45	56334.0
46	56601.5
47	56869.0
48	56875.5
49	56882.0
50	52525.5
51	48169.0
52	43014.5
53	37860.0
54	33271.0
55	28682.0
56	25952.5
57	23223.0
58	21252.5
59	19282.0
60	15540.0
61	11798.0
62	10245.5
63	8693.0
64	7129.0
65	5565.0
66	4517.5
67	3470.0
68	3211.0
69	2952.0
70	2634.5
71	2317.0
72	2070.0
73	1823.0
74	1559.5
75	1037.0
76	778.0
77	599.5
78	421.0
79	377.5
80	334.0
81	217.5
82	101.0
83	79.5
84	58.0
85	41.5
86	25.0
87	16.5
88	8.0
89	7.5
90	7.0
91	3.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	755370.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.53283038709573
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.65870776897513	27.949665216231224
2	9.234478713566958	6.562543316848414
3	3.427709607974959	3.653886723491779
4	1.836401348519643	2.6101015055832937
5	1.1222230744777635	1.9937881080951736
6	0.782287608081196	1.6678135735125574
7	0.5550956473987908	1.3806883641345433
8	0.4384299443421148	1.246292547914575
9	0.3176775298161441	1.0159183606273734
>10	2.9373737816998773	23.45378625374902
>50	0.5667591982009766	13.67568733297414
>100	0.11783337097065355	6.1951806645776015
>500	0.003477050290937318	0.8367042511600186
>1k	7.726778424305149E-4	0.42089175292151226
>5k	3.8633892121525746E-4	0.6899549739672278
>10k+	3.8633892121525746E-4	6.647097054211527
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	48421	6.410236043263566	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5026	0.6653692892225003	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1988	0.2631822815309054	No Hit
GCGAAGGGTCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCACAAGCCA	1078	0.14271151885830785	No Hit
CTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	911	0.12060314812608391	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.10259872645193746	0.0
2	0.0	0.0	0.0	0.3296397791810636	0.0
3	0.0	0.0	0.0	0.5357639302593431	0.0
4	0.0	0.0	0.0	0.672782874617737	0.0
5	0.0	0.0	0.0	1.4948965407681003	0.0
6	0.0	0.0	0.0	1.7195546553344718	0.0
7	0.0	0.0	0.0	2.384129631836054	0.0
8	0.0	0.0	0.0	3.130518818592213	0.0
9	0.0	0.0	0.0	3.6964666322464486	0.0
10	0.0	0.0	0.0	4.086606563670784	0.0
11	0.0	0.0	0.0	4.552338589035837	0.0
12	0.0	0.0	0.0	4.9166633570303295	0.0
13	0.0	0.0	0.0	5.088234904748666	0.0
14	0.0	0.0	0.0	5.2206203582350375	0.0
15	0.0	0.0	0.0	5.681851278181553	0.0
16	0.0	0.0	0.0	6.223837324754756	0.0
17	0.0	0.0	0.0	6.886426519454043	0.0
18	0.0	0.0	0.0	7.210373724135192	0.0
19	0.0	0.0	0.0	7.599454571931636	0.0
20	0.0	0.0	0.0	8.028912983041423	0.0
21	1.323854534863709E-4	0.0	0.0	8.439175503395687	0.0
22	1.323854534863709E-4	0.0	0.0	8.873399790830984	0.0
23	3.9715636045911277E-4	0.0	0.0	9.23097290069767	0.0
24	0.0019857818022955636	0.0	0.0	9.536650912797702	0.0
25	0.0019857818022955636	0.0	0.0	9.82816368137469	0.0
26	0.0019857818022955636	0.0	0.0	10.14482968611409	0.0
27	0.0019857818022955636	0.0	0.0	10.443756040086315	0.0
28	0.0021181672557819345	0.0	0.0	10.759362961197823	0.0
29	0.0021181672557819345	0.0	0.0	11.15890225981969	0.0
30	0.0021181672557819345	0.0	0.0	11.531567311383824	0.0
31	0.0021181672557819345	0.0	0.0	11.864913883262506	0.0
32	0.0021181672557819345	0.0	0.0	12.188199160676225	0.0
33	0.0021181672557819345	0.0	0.0	12.53743198697327	0.0
34	0.0021181672557819345	0.0	0.0	12.948753590955425	0.0
35	0.0021181672557819345	0.0	0.0	13.296397791810636	0.0
36	0.0021181672557819345	0.0	0.0	13.62855289460794	0.0
37	0.0022505527092683054	0.0	0.0	13.99446628804427	0.0
38	0.0022505527092683054	0.0	0.0	14.3691171214107	0.0
39	0.0022505527092683054	0.0	0.0	14.747209976567774	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTACGG	60	0.0	45.000004	1
CTAACGG	70	0.0	45.000004	1
CCCCGAA	30	2.16451E-6	45.000004	24
CGCTAAG	30	2.16451E-6	45.000004	9
TACCTCC	35	1.2111923E-7	45.000004	10
ATAACGG	30	2.16451E-6	45.000004	1
TCGGTAG	30	2.16451E-6	45.000004	27
GTCGGTT	35	1.2111923E-7	45.000004	37
AAGTAAC	20	7.031749E-4	45.0	15
CTATCCG	25	3.8895316E-5	45.0	15
GACGTGT	45	3.8380676E-10	45.0	43
TTATGCA	20	7.031749E-4	45.0	12
ATTACGT	20	7.031749E-4	45.0	33
TAAACGG	40	6.8102963E-9	45.0	1
GCTGCGC	25	3.8895316E-5	45.0	15
TGACGGA	20	7.031749E-4	45.0	2
TGCGAGC	20	7.031749E-4	45.0	3
AGGCACG	20	7.031749E-4	45.0	19
ACAATTG	40	6.8102963E-9	45.0	45
ACTACGG	40	6.8102963E-9	45.0	1
>>END_MODULE