##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043438_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2866663
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.50110215257252	31.0	31.0	33.0	30.0	34.0
2	30.54385813749297	31.0	31.0	34.0	26.0	34.0
3	31.52108043394009	31.0	31.0	34.0	28.0	34.0
4	35.150269145693095	37.0	35.0	37.0	32.0	37.0
5	35.46931153051475	37.0	35.0	37.0	33.0	37.0
6	35.73500721919528	37.0	35.0	37.0	35.0	37.0
7	33.70793567294098	37.0	35.0	37.0	32.0	37.0
8	34.71376509900187	37.0	35.0	37.0	31.0	37.0
9	36.74002071397998	39.0	37.0	39.0	32.0	39.0
10	36.70429066827876	39.0	35.0	39.0	32.0	39.0
11	36.45424418566117	39.0	35.0	39.0	32.0	39.0
12	36.447868130994124	39.0	35.0	39.0	32.0	39.0
13	36.45603825772335	39.0	35.0	39.0	32.0	39.0
14	37.64886245784733	40.0	35.0	41.0	33.0	41.0
15	37.723359529878465	40.0	35.0	41.0	33.0	41.0
16	37.514390076545446	40.0	35.0	41.0	32.0	41.0
17	37.455845350499864	40.0	35.0	41.0	32.0	41.0
18	37.27979605555309	39.0	35.0	41.0	32.0	41.0
19	37.11911515235659	38.0	35.0	41.0	32.0	41.0
20	36.86665506200066	38.0	35.0	41.0	32.0	41.0
21	36.77203145259837	38.0	35.0	40.0	32.0	41.0
22	36.715020914561634	38.0	35.0	40.0	32.0	41.0
23	36.65238571816778	38.0	35.0	40.0	32.0	41.0
24	36.53016486416436	38.0	35.0	40.0	31.0	41.0
25	36.408800755442826	38.0	35.0	40.0	31.0	41.0
26	36.12079654985605	37.0	35.0	40.0	31.0	41.0
27	36.01193094549307	37.0	35.0	40.0	30.0	41.0
28	35.838325955998314	37.0	35.0	40.0	30.0	41.0
29	35.71196684088782	37.0	35.0	40.0	30.0	41.0
30	35.54065092408839	37.0	34.0	40.0	29.0	41.0
31	35.31923424553217	37.0	34.0	40.0	28.0	41.0
32	35.187018146186	37.0	34.0	40.0	27.0	41.0
33	34.87841961193206	37.0	34.0	40.0	25.0	41.0
34	34.72991000337326	37.0	34.0	40.0	24.0	41.0
35	34.591390058754726	37.0	34.0	40.0	23.0	41.0
36	34.4921733039426	36.0	34.0	40.0	23.0	41.0
37	34.35668475855027	36.0	34.0	40.0	23.0	41.0
38	34.22852389694917	36.0	34.0	40.0	23.0	41.0
39	34.10141757158061	36.0	33.0	40.0	22.0	41.0
40	33.99916941754228	35.0	33.0	40.0	22.0	41.0
41	33.906334996474996	35.0	33.0	40.0	22.0	41.0
42	33.78177448831621	35.0	33.0	40.0	22.0	41.0
43	33.60139681574012	35.0	33.0	40.0	21.0	41.0
44	33.377346064047295	35.0	33.0	40.0	20.0	41.0
45	33.16327451116507	35.0	33.0	39.0	20.0	41.0
46	33.14494169701845	35.0	33.0	39.0	20.0	41.0
47	33.11955992036734	35.0	33.0	39.0	20.0	41.0
48	33.02455886862181	35.0	33.0	39.0	20.0	41.0
49	32.979678462379425	35.0	33.0	39.0	20.0	41.0
50	32.9047181339418	35.0	33.0	39.0	20.0	41.0
51	32.47838549560935	35.0	32.0	38.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	2.0
10	1.0
11	11.0
12	21.0
13	28.0
14	61.0
15	170.0
16	365.0
17	838.0
18	1866.0
19	3411.0
20	5477.0
21	8659.0
22	13384.0
23	19841.0
24	29715.0
25	43752.0
26	57521.0
27	67977.0
28	72824.0
29	76954.0
30	84614.0
31	97049.0
32	115020.0
33	151361.0
34	275586.0
35	288098.0
36	286455.0
37	312887.0
38	383137.0
39	469343.0
40	232.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.339549155237293	17.175231270644648	23.381925255950907	30.10329431816715
2	32.13073877187517	19.9761534578707	26.35370812683598	21.539399643418147
3	25.75381201068978	21.35088777439134	33.37762408765872	19.517676127260163
4	23.43362299649453	24.137891339163343	30.614411250991136	21.814074413350994
5	21.304910971397753	33.316891451837904	29.00860687147391	16.369590705290438
6	81.41972042057263	2.1662469568275027	15.04557738387805	1.368455238721817
7	77.09552884311829	7.337102407921685	14.122448296154797	1.4449204528052304
8	76.21199282929315	3.66129538072665	17.024079914520822	3.102631875459376
9	45.34209287942112	17.32519657873981	22.919889781254373	14.412820760584694
10	30.889434858579467	18.305883879618918	29.696514728100233	21.108166533701382
11	27.47605839960958	15.006298263869871	38.228769827496286	19.288873509024256
12	23.290146068791483	16.99334731707215	38.255386140610184	21.461120473526186
13	19.238606002868146	19.88036263767314	41.14829681758895	19.73273454186976
14	19.915630124643183	18.090581278650472	41.3301458873959	20.66364270931044
15	24.99885755667827	15.125496090750815	39.55271338137758	20.322932971193335
16	24.226984476375492	16.195555598966465	36.13853459580007	23.43892532885798
17	24.56301978990903	18.943105624902543	35.93812736272105	20.555747222467378
18	24.93878771240289	22.983064280663616	32.42637868490297	19.65176932203053
19	27.220639468259783	21.1057595538785	31.33995869064484	20.33364228721688
20	26.362289533161032	23.048122503412504	33.48374050245878	17.10584746096768
21	23.282122802715215	18.604977285436064	35.48062677754588	22.632273134302846
22	25.085508830301993	20.18221186096866	34.1789041823193	20.553375126410046
23	26.180545114650727	19.216838533165564	34.9134167497191	19.68919960246461
24	27.143825416520883	17.349057074375327	32.377715831962114	23.12940167714168
25	21.780551114658405	19.1404779703788	34.26796243576591	24.811008479196893
26	19.1545012441295	19.431896947775165	36.11533689170997	25.29826491638536
27	20.87974763688651	22.19751676426563	35.228521803923236	21.69421379492462
28	24.041960983903586	22.80690126464115	32.32099482917943	20.830142922275833
29	22.877819960002274	20.153258335562988	36.437209396430625	20.531712308004117
30	28.15789648103038	20.016374439548702	32.1122503761342	19.713478703286714
31	28.58787377518739	20.321572504336924	30.819702211247012	20.270851509228674
32	28.147640653958977	19.639036747605143	30.36286441761728	21.850458180818602
33	23.781483906549184	21.97255136024011	33.670333764380395	20.575630968830307
34	23.170145915302914	24.017786534378125	32.01419211117595	20.797875439143006
35	25.586300168523472	27.05539507085416	28.381187464309548	18.97711729631282
36	25.591916454776857	25.31434633230345	27.801454164650675	21.292283048269013
37	25.09838093978957	28.763025162008926	26.394243062403916	19.74435083579758
38	25.138043781218787	23.963577162714973	27.2375929783166	23.660786077749634
39	23.852542136972502	21.932504797389857	31.19683060059728	23.01812246504036
40	21.66564399093999	23.338041478890265	31.147225885986597	23.84908864418315
41	21.482887943228764	25.99283557223155	27.58381435139045	24.94046213314924
42	20.917736057569375	21.31701563804326	29.766142724136042	27.99910558025132
43	22.15024926194673	20.214235157742642	32.09543640113958	25.540079179171045
44	24.23270541392553	22.613052179485347	30.39558538970224	22.758657016886882
45	25.04929948166213	23.320843782474608	30.244782871233905	21.385073864629362
46	22.18485395737134	24.185333260309985	30.111945492023306	23.51786729029537
47	21.410155292059095	25.291009093151168	31.614354390453293	21.684481224336448
48	23.887530553818152	23.90117010614781	30.889120904689527	21.32217843534451
49	22.013330482166896	22.138214362832326	32.805355913827334	23.043099241173447
50	21.045759477134215	23.010866641806167	33.874927049325294	22.06844683173432
51	20.816573137477267	20.763689348904983	33.30489143648905	25.114846077128703
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1238.0
1	12677.5
2	24117.0
3	90838.5
4	157560.0
5	96106.5
6	34653.0
7	30807.5
8	26962.0
9	26731.5
10	26501.0
11	26513.5
12	26526.0
13	25215.5
14	23905.0
15	22055.5
16	20206.0
17	18884.5
18	17563.0
19	16205.0
20	14847.0
21	14348.5
22	13850.0
23	13706.5
24	13563.0
25	13727.0
26	15484.5
27	17078.0
28	18203.0
29	19328.0
30	22129.5
31	24931.0
32	28579.0
33	32227.0
34	36747.5
35	41268.0
36	44663.5
37	48059.0
38	50304.0
39	52549.0
40	58718.0
41	64887.0
42	75667.5
43	86448.0
44	92682.0
45	98916.0
46	105740.5
47	112565.0
48	132192.5
49	151820.0
50	156479.5
51	161139.0
52	156256.5
53	151374.0
54	155244.0
55	159114.0
56	166087.5
57	173061.0
58	179181.5
59	185302.0
60	189837.5
61	194373.0
62	187856.5
63	181340.0
64	167791.0
65	154242.0
66	131200.5
67	108159.0
68	88781.0
69	69403.0
70	60182.5
71	50962.0
72	48789.0
73	46616.0
74	39219.5
75	24236.0
76	16649.0
77	12751.5
78	8854.0
79	6633.0
80	4412.0
81	3360.0
82	2308.0
83	1800.0
84	1292.0
85	891.5
86	491.0
87	332.5
88	174.0
89	118.5
90	63.0
91	46.0
92	29.0
93	22.0
94	15.0
95	11.5
96	8.0
97	5.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2866663.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.839638136832676
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.23018874928357	19.956001305207735
2	8.34705959096363	4.313699986741575
3	2.8091408006763725	2.177615452946696
4	1.497629167019368	1.5479278295578647
5	0.965792605897387	1.2477865725808566
6	0.71063654007328	1.1017554625382608
7	0.5735566696941753	1.0374347757105047
8	0.4499600382725359	0.9301443651998165
9	0.3958226014934344	0.9205121510073064
>10	5.857216657446914	35.918137810508746
>50	1.0154081110554698	17.27026854454966
>100	0.13866252147848426	5.560813011453864
>500	0.005758675255091788	0.9947047359597283
>1k	0.0024474369834140103	1.407965325801406
>5k	2.8793376275458945E-4	0.5452108384840786
>10k+	4.3190064413188415E-4	5.0700218317519505
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	102446	3.5737022454331044	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	23003	0.8024312589237033	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10840	0.3781400185511865	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	8212	0.2864654826884081	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	6444	0.22479098519777177	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	4366	0.1523025203869447	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4352	0.15181414766925863	No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	4188	0.14609321011922224	No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	4150	0.14476762702836016	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.030488411089828138	0.0
2	0.0	0.0	0.0	0.09948849934575497	0.0
3	0.0	0.0	0.0	0.16287230134829242	0.0
4	0.0	0.0	0.0	0.2041049122272133	0.0
5	0.0	0.0	0.0	0.4405121913528029	0.0
6	0.0	0.0	0.0	0.5438727886744972	0.0
7	0.0	0.0	0.0	0.8069661484450736	0.0
8	0.0	0.0	0.0	1.1095130470515717	0.0
9	0.0	0.0	0.0	1.4000948140747622	0.0
10	0.0	0.0	0.0	1.5735717801499514	0.0
11	0.0	0.0	0.0	1.7389208288522229	0.0
12	0.0	0.0	0.0	1.8703628574408642	0.0
13	0.0	0.0	0.0	1.9400955047733202	0.0
14	0.0	0.0	0.0	1.9930141771111567	0.0
15	0.0	0.0	0.0	2.134467846412362	0.0
16	0.0	0.0	0.0	2.2893517654499327	0.0
17	0.0	0.0	0.0	2.5015148275189656	0.0
18	0.0	0.0	0.0	2.620433584275515	0.0
19	0.0	0.0	0.0	2.753759336203802	0.0
20	0.0	0.0	0.0	2.907980463695942	0.0
21	3.4883765549002444E-5	0.0	0.0	3.0744806766613304	0.0
22	3.4883765549002444E-5	0.0	0.0	3.237666931899564	0.0
23	6.976753109800489E-5	0.0	0.0	3.389620614631019	0.0
24	6.976753109800489E-5	0.0	0.0	3.5200510140187387	0.0
25	6.976753109800489E-5	0.0	0.0	3.653167463353732	0.0
26	6.976753109800489E-5	0.0	0.0	3.7964699722290343	0.0
27	6.976753109800489E-5	0.0	0.0	3.9479352822428027	0.0
28	6.976753109800489E-5	0.0	0.0	4.104040133074589	0.0
29	6.976753109800489E-5	0.0	0.0	4.265586851332019	0.0
30	1.0465129664700734E-4	0.0	0.0	4.4281103150248216	0.0
31	1.0465129664700734E-4	0.0	0.0	4.600261698009149	0.0
32	1.0465129664700734E-4	0.0	0.0	4.805692193327224	0.0
33	1.0465129664700734E-4	0.0	0.0	5.005262216033067	0.0
34	1.0465129664700734E-4	0.0	0.0	5.224332263680802	0.0
35	1.0465129664700734E-4	0.0	0.0	5.422297633171391	0.0
36	1.0465129664700734E-4	0.0	0.0	5.646739780713673	0.0
37	1.0465129664700734E-4	0.0	0.0	5.941821553492685	0.0
38	1.0465129664700734E-4	0.0	0.0	6.243914963147046	0.0
39	1.0465129664700734E-4	0.0	0.0	6.610857292957003	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCGCTA	20	7.035053E-4	45.0	20
CGTTTTT	28940	0.0	43.91154	1
TATACGG	200	0.0	42.749996	1
TCGATTA	275	0.0	41.727272	40
TACGCGG	320	0.0	41.484375	1
CGTTTTA	1520	0.0	40.70724	1
CACTTAC	6945	0.0	40.561554	36
ACTTACT	6915	0.0	40.5423	37
CATGCGG	550	0.0	40.5	1
CGCACTT	6950	0.0	40.5	34
TAGTAGG	590	0.0	40.42373	1
ACCCGCA	6985	0.0	40.297066	31
CTTACTG	6935	0.0	40.263157	38
GCACTTA	6985	0.0	40.232643	35
CTAGCGG	840	0.0	40.178574	1
CCCGCAC	7130	0.0	39.730015	32
GGCGATA	500	0.0	39.600002	7
GGTACCT	2070	0.0	39.56522	7
CCGCACT	7115	0.0	39.560787	33
GGGTACC	2245	0.0	39.387527	6
>>END_MODULE