FastQCFastQC Report
Thu 26 May 2016
SRR1043437_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043437_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3360044
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1276923.800307376927207No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC248040.7382046187490402No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134310.3997269083381051No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC91930.273597607650376No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC69320.20630682217256677No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56400.1678549447566758No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA53030.15782531419231416No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC38250.11383779498125621No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC37210.11074259741836714No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCGAT207.035227E-445.018
CGTTTTT368650.043.828161
TCACGAC6350.040.393724
CACGACG6300.040.35714325
ACGGGTA3000.039.7500044
CGTTTTA20950.039.737471
TAGCATA20800.039.69951629
CGCACTT76600.039.6834234
CCGCACT76850.039.55432533
CTTACTG76650.039.45205738
AACACGT4050.039.44444740
ACCCGCA77000.039.4188331
ACGTTAG403.4608638E-739.37520
TTCCGTA3550.039.29577345
CACTTAC77850.039.19075436
GCACTTA77500.039.13548735
CCCGCAC77850.039.07514632
ACTTACT78000.038.88461337
AGGGTAC9900.038.8636365
CCAGTAG21950.038.5421425