##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043437_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3360044
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.603041805404928	31.0	31.0	33.0	30.0	34.0
2	30.623188863002984	31.0	31.0	34.0	26.0	34.0
3	31.596814208385368	31.0	31.0	34.0	28.0	34.0
4	35.21119187724923	37.0	35.0	37.0	33.0	37.0
5	35.53420907583353	37.0	35.0	37.0	33.0	37.0
6	35.81767203048531	37.0	35.0	37.0	35.0	37.0
7	33.74027423450407	37.0	35.0	37.0	32.0	37.0
8	34.772987198977155	37.0	35.0	37.0	32.0	37.0
9	36.86053664773438	39.0	37.0	39.0	32.0	39.0
10	36.827425771805366	39.0	35.0	39.0	32.0	39.0
11	36.60294686617199	39.0	35.0	39.0	33.0	39.0
12	36.59782639751146	39.0	35.0	39.0	33.0	39.0
13	36.610967892087125	39.0	35.0	39.0	33.0	39.0
14	37.82303535310847	40.0	36.0	41.0	33.0	41.0
15	37.89665581760239	40.0	36.0	41.0	33.0	41.0
16	37.720593539846504	40.0	36.0	41.0	32.0	41.0
17	37.65499023227077	40.0	36.0	41.0	32.0	41.0
18	37.44399686432678	39.0	36.0	41.0	32.0	41.0
19	37.257008241558744	38.0	35.0	41.0	32.0	41.0
20	36.98662398468591	38.0	35.0	41.0	32.0	41.0
21	36.888596994563166	38.0	35.0	41.0	32.0	41.0
22	36.8110670574552	38.0	35.0	40.0	32.0	41.0
23	36.77262381087867	38.0	35.0	40.0	32.0	41.0
24	36.64954566071159	38.0	35.0	40.0	32.0	41.0
25	36.53759474578309	38.0	35.0	40.0	31.0	41.0
26	36.265525391929394	38.0	35.0	40.0	31.0	41.0
27	36.15164801413315	37.0	35.0	40.0	31.0	41.0
28	35.979631219114985	37.0	35.0	40.0	30.0	41.0
29	35.83926371202282	37.0	35.0	40.0	30.0	41.0
30	35.658318462496325	37.0	34.0	40.0	30.0	41.0
31	35.46135824411823	37.0	34.0	40.0	29.0	41.0
32	35.324402894723995	37.0	34.0	40.0	27.0	41.0
33	34.968700112260436	37.0	34.0	40.0	25.0	41.0
34	34.81311375684366	37.0	34.0	40.0	24.0	41.0
35	34.66658472329529	37.0	34.0	40.0	23.0	41.0
36	34.56044682748202	37.0	34.0	40.0	23.0	41.0
37	34.43531245424167	36.0	34.0	40.0	23.0	41.0
38	34.30066005088029	36.0	34.0	40.0	23.0	41.0
39	34.17480366328536	36.0	34.0	40.0	22.0	41.0
40	34.06905415524321	36.0	33.0	40.0	22.0	41.0
41	33.96591294637808	35.0	33.0	40.0	22.0	41.0
42	33.84513536132265	35.0	33.0	40.0	21.0	41.0
43	33.66615526463344	35.0	33.0	40.0	21.0	41.0
44	33.43933085400072	35.0	33.0	40.0	20.0	41.0
45	33.22329499256557	35.0	33.0	40.0	20.0	41.0
46	33.22073252612168	35.0	33.0	39.0	20.0	41.0
47	33.181985712091866	35.0	33.0	39.0	20.0	41.0
48	33.091245531308516	35.0	33.0	39.0	20.0	41.0
49	33.03656321167223	35.0	33.0	39.0	20.0	41.0
50	32.96961260031119	35.0	33.0	39.0	20.0	41.0
51	32.5375504011257	35.0	32.0	38.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	8.0
10	2.0
11	6.0
12	12.0
13	28.0
14	75.0
15	142.0
16	353.0
17	837.0
18	1664.0
19	3060.0
20	5508.0
21	8713.0
22	13622.0
23	21341.0
24	32782.0
25	48739.0
26	66357.0
27	79313.0
28	85631.0
29	89546.0
30	96879.0
31	110611.0
32	132792.0
33	175428.0
34	319903.0
35	326235.0
36	338907.0
37	369233.0
38	458243.0
39	573751.0
40	320.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.673672130484007	16.33868485055553	23.477192560573613	31.510450458386856
2	32.800314519690815	19.501738667707926	26.519444388228248	21.178502424373015
3	25.84903650071249	20.794013411729132	34.25931327089764	19.097636816660735
4	23.67370784430204	23.45469285521261	31.37393438895443	21.49766491153092
5	21.58433044329181	32.22008402270923	29.833775986266847	16.36180954773211
6	80.19014036720947	2.0870560028380583	16.26791196781947	1.454891662132996
7	75.8650184342824	7.337017015253372	15.20980082403683	1.588163726427392
8	75.05074338312237	3.5956671995961953	18.196190287984322	3.1573991292971164
9	45.10667122216256	16.898201333077782	23.660225877994453	14.334901566765195
10	30.013535537034635	17.638757111514018	31.35125611450326	20.99645123694809
11	26.688906454796424	14.665968659934215	39.255021660430636	19.39010322483872
12	22.413486251965747	15.773692249268164	40.12417694530191	21.688644553464183
13	18.540828632006008	18.70353483466288	43.31481968688505	19.440816846446058
14	19.537512008771312	17.237929027119883	42.7074169266831	20.5171420374257
15	24.503756498426807	14.687902896509689	40.50759454340479	20.300746061658717
16	24.303640071380016	15.833006948718529	37.17775719603672	22.685595783864734
17	23.965251645514165	18.687761231698154	37.18689398115025	20.160093141637432
18	24.42087067907444	21.914415406464915	33.73059995642914	19.934113958031503
19	26.266233418371904	20.415238609970583	32.88251582419754	20.436012147459973
20	26.123884091994032	21.71507277880885	35.12471860487541	17.036324524321707
21	23.452788118250833	17.480366328536174	36.958295784221875	22.10854976899112
22	24.5443512049247	19.2466229608898	35.44233349325188	20.76669234093363
23	25.844929411638656	18.33728963073103	35.63453335730127	20.183247600329043
24	26.507688589792277	16.557997454795235	33.776135074421646	23.158178880990842
25	21.96259929929489	18.334878948013774	35.41545884518179	24.287062907509544
26	19.7124501941046	18.97302535323942	36.729013072447856	24.585511380208118
27	21.15644914173743	21.191865344620485	35.83967948038776	21.812006033254328
28	23.939269842894912	22.034235265966757	33.58682207732994	20.43967281380839
29	22.609436066908646	19.48206630627456	37.66159014584333	20.246907480973462
30	27.7864813675059	19.361829785562332	33.27920705800281	19.572481788928954
31	28.08722147686161	19.862061330149245	32.239964714747785	19.810752478241355
32	27.537556055813557	19.74518786063516	31.41426124181707	21.302994841734215
33	23.31008165369263	21.20427589638707	35.14114696117075	20.344495488749555
34	23.350616837160466	23.633767891134756	32.758737683197005	20.25687758850777
35	25.35797150275413	26.432600287377188	29.59089226212514	18.618535947743542
36	25.603712332338507	24.443727522615774	28.91634752402052	21.036212621025204
37	24.84774008911788	28.08873931412803	27.381962855248325	19.681557741505763
38	24.796193145089767	23.597815980981203	28.116000861893475	23.489990012035555
39	23.5340668157917	21.3132625644188	31.888897883480098	23.263772736309406
40	21.799416912397575	22.398754302026997	31.96993848890074	23.831890296674686
41	21.299691313566132	25.072677619697835	28.633375039136393	24.994256027599636
42	20.868387437783554	21.259423983733548	30.32144817151204	27.55074040697086
43	21.893195446250107	20.16604544464299	32.91587848254368	25.024880626563224
44	24.192748666386514	21.99262866795792	31.515628962001692	22.29899370365388
45	24.341586003040437	22.95341965759972	31.293072352623952	21.411921986735887
46	22.46628317962503	22.963598095739222	31.073670463839164	23.496448260796583
47	21.562247399141203	24.583070935975837	32.51615157420558	21.338530090677384
48	23.784986148990907	23.320260091832132	31.37512484955554	21.51962890962142
49	21.945278097548723	21.452605977778862	33.877205179455984	22.72491074521643
50	21.2203768760171	22.151078973965816	34.731658275903534	21.896885874113554
51	20.724460751109213	20.519850335293228	33.65976755066303	25.095921362934533
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1130.0
1	15941.0
2	30752.0
3	116861.0
4	202970.0
5	123401.0
6	43832.0
7	38750.5
8	33669.0
9	32538.5
10	31408.0
11	30808.0
12	30208.0
13	28748.0
14	27288.0
15	25129.0
16	22970.0
17	21362.5
18	19755.0
19	18973.5
20	18192.0
21	17558.5
22	16925.0
23	16752.5
24	16580.0
25	16781.0
26	18219.0
27	19456.0
28	21121.0
29	22786.0
30	25757.0
31	28728.0
32	31376.5
33	34025.0
34	40407.0
35	46789.0
36	49714.0
37	52639.0
38	57179.5
39	61720.0
40	69964.0
41	78208.0
42	88233.0
43	98258.0
44	112993.5
45	127729.0
46	139944.0
47	152159.0
48	172056.0
49	191953.0
50	186240.0
51	180527.0
52	168733.5
53	156940.0
54	162123.0
55	167306.0
56	181000.5
57	194695.0
58	204524.5
59	214354.0
60	214934.5
61	215515.0
62	214208.5
63	212902.0
64	199591.0
65	186280.0
66	157821.5
67	129363.0
68	106894.5
69	84426.0
70	73037.0
71	61648.0
72	57323.0
73	52998.0
74	44290.5
75	27801.0
76	20019.0
77	14950.0
78	9881.0
79	7673.0
80	5465.0
81	4145.0
82	2825.0
83	2025.5
84	1226.0
85	923.5
86	621.0
87	424.0
88	227.0
89	148.0
90	69.0
91	49.5
92	30.0
93	24.0
94	18.0
95	16.5
96	15.0
97	7.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3360044.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.985802972346857
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.38121046898166	19.848270860363144
2	8.792057393060109	4.569373422752509
3	2.766187251347071	2.1564479069436806
4	1.4887097012553494	1.5474126791937142
5	0.9500452059551159	1.2343843768386165
6	0.7504072962872042	1.1699961690198477
7	0.5787189302782207	1.0526933269004026
8	0.5087784259671692	1.0576812747010893
9	0.42835461771994277	1.001802482852885
>10	6.386652430253413	38.946260638695044
>50	0.8468559899144393	14.291054794759955
>100	0.1122696441938139	4.692425572249439
>500	0.0059734949316403805	1.0371952055915643
>1k	0.002925793435905372	1.2811535537867724
>5k	4.87632239317562E-4	0.8574814399362137
>10k+	3.657241794881715E-4	5.256366295415107
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	127692	3.800307376927207	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	24804	0.7382046187490402	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13431	0.3997269083381051	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	9193	0.273597607650376	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	6932	0.20630682217256677	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5640	0.1678549447566758	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	5303	0.15782531419231416	No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	3825	0.11383779498125621	No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	3721	0.11074259741836714	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.041725644068946716	0.0
2	0.0	0.0	0.0	0.13083162006211824	0.0
3	0.0	0.0	0.0	0.20940201973545586	0.0
4	0.0	0.0	0.0	0.2633298849657921	0.0
5	0.0	0.0	0.0	0.5790995594105315	0.0
6	0.0	0.0	0.0	0.7298713945412619	0.0
7	0.0	0.0	0.0	1.106533128732838	0.0
8	0.0	0.0	0.0	1.5343846687721947	0.0
9	0.0	0.0	0.0	1.9422067091978557	0.0
10	0.0	0.0	0.0	2.1935427036074526	0.0
11	0.0	0.0	0.0	2.4180338114619926	0.0
12	0.0	0.0	0.0	2.6023766355440583	0.0
13	0.0	0.0	0.0	2.7028217487628137	0.0
14	0.0	0.0	0.0	2.769725634545262	0.0
15	0.0	0.0	0.0	2.9380270020273542	0.0
16	0.0	0.0	0.0	3.1161199079535864	0.0
17	0.0	0.0	0.0	3.3687951705394332	0.0
18	0.0	0.0	0.0	3.508882621775191	0.0
19	0.0	0.0	0.0	3.678731588038728	0.0
20	0.0	0.0	0.0	3.8695624223968497	0.0
21	0.0	0.0	0.0	4.075214491238805	0.0
22	2.9761515027779398E-5	0.0	0.0	4.27524163374051	0.0
23	2.9761515027779398E-5	0.0	0.0	4.452858355426298	0.0
24	2.9761515027779398E-5	0.0	0.0	4.602350445410834	0.0
25	2.9761515027779398E-5	0.0	0.0	4.757824599915954	0.0
26	2.9761515027779398E-5	0.0	0.0	4.920828417723102	0.0
27	2.9761515027779398E-5	0.0	0.0	5.096093979721694	0.0
28	2.9761515027779398E-5	0.0	0.0	5.271865487475759	0.0
29	2.9761515027779398E-5	0.0	0.0	5.458559471245019	0.0
30	2.9761515027779398E-5	0.0	0.0	5.646384392585335	0.0
31	2.9761515027779398E-5	0.0	0.0	5.8471853344777625	0.0
32	2.9761515027779398E-5	0.0	0.0	6.075962100496303	0.0
33	2.9761515027779398E-5	0.0	0.0	6.301911522587204	0.0
34	2.9761515027779398E-5	0.0	0.0	6.554914161838357	0.0
35	2.9761515027779398E-5	0.0	0.0	6.795952672048342	0.0
36	2.9761515027779398E-5	0.0	0.0	7.05211003189244	0.0
37	2.9761515027779398E-5	0.0	0.0	7.393444847746041	0.0
38	2.9761515027779398E-5	0.0	0.0	7.74314264932245	0.0
39	2.9761515027779398E-5	0.0	0.0	8.140220782823082	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTCGAT	20	7.035227E-4	45.0	18
CGTTTTT	36865	0.0	43.82816	1
TCACGAC	635	0.0	40.3937	24
CACGACG	630	0.0	40.357143	25
ACGGGTA	300	0.0	39.750004	4
CGTTTTA	2095	0.0	39.73747	1
TAGCATA	2080	0.0	39.699516	29
CGCACTT	7660	0.0	39.68342	34
CCGCACT	7685	0.0	39.554325	33
CTTACTG	7665	0.0	39.452057	38
AACACGT	405	0.0	39.444447	40
ACCCGCA	7700	0.0	39.41883	31
ACGTTAG	40	3.4608638E-7	39.375	20
TTCCGTA	355	0.0	39.295773	45
CACTTAC	7785	0.0	39.190754	36
GCACTTA	7750	0.0	39.135487	35
CCCGCAC	7785	0.0	39.075146	32
ACTTACT	7800	0.0	38.884613	37
AGGGTAC	990	0.0	38.863636	5
CCAGTAG	2195	0.0	38.54214	25
>>END_MODULE