FastQCFastQC Report
Thu 26 May 2016
SRR1043436_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043436_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3973733
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1042932.624559828252175No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC406861.0238735214469619No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC157080.395295808752123No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC120010.3020082124289679No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109990.2767926279898524No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC69530.1749740105840025No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA55310.13918901949376064No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC51810.13038118061782208No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43700.10997215967957585No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACACG551.8189894E-1245.019
CGTTTTT309300.043.676041
CGGTCTA10150.041.8965530
GCACTTA116250.041.69032335
CACTTAC116300.041.6530536
CGCACTT116950.041.59469634
ACTTACT116400.041.5012937
CCCGCAC118250.041.49894332
CTTACTG116700.041.49138
CCGCACT117550.041.4015333
ACCCGCA117850.041.3725131
TATACGG2300.041.0869561
TCACGAC10850.040.23041524
TTATGAC124100.040.17727726
TATGACC123500.040.17206627
GTAGGGT14900.040.1677863
TCAACGC127150.040.11600515
AACGCAA126650.040.1144917
GCTTATG125400.040.02990324
CAACGCA127150.040.02752716