##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043436_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3973733
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.542442836496562	31.0	31.0	33.0	30.0	34.0
2	30.590750057942998	31.0	31.0	34.0	26.0	34.0
3	31.58466610615258	31.0	31.0	34.0	28.0	34.0
4	35.20029151430154	37.0	35.0	37.0	33.0	37.0
5	35.51753552641811	37.0	35.0	37.0	33.0	37.0
6	35.74975998639063	37.0	35.0	37.0	35.0	37.0
7	33.71473599257927	37.0	35.0	37.0	32.0	37.0
8	34.7339811205232	37.0	35.0	37.0	31.0	37.0
9	36.73006641362165	39.0	37.0	39.0	32.0	39.0
10	36.69806124367188	39.0	35.0	39.0	32.0	39.0
11	36.4794232526443	39.0	35.0	39.0	32.0	39.0
12	36.47563563027511	39.0	35.0	39.0	33.0	39.0
13	36.50027140726365	39.0	35.0	39.0	33.0	39.0
14	37.657973497464475	40.0	35.0	41.0	33.0	41.0
15	37.74024525553176	40.0	35.0	41.0	33.0	41.0
16	37.522244197081186	40.0	35.0	41.0	32.0	41.0
17	37.47332495665914	40.0	35.0	41.0	32.0	41.0
18	37.37232597157383	39.0	35.0	41.0	32.0	41.0
19	37.24974878785263	39.0	35.0	41.0	32.0	41.0
20	36.99005242677352	38.0	35.0	41.0	32.0	41.0
21	36.937568276479574	38.0	35.0	41.0	32.0	41.0
22	36.88100660009115	38.0	35.0	41.0	32.0	41.0
23	36.83718382689526	38.0	35.0	40.0	32.0	41.0
24	36.73658496934746	38.0	35.0	40.0	32.0	41.0
25	36.63061131686502	38.0	35.0	40.0	31.0	41.0
26	36.359821356895395	38.0	35.0	40.0	31.0	41.0
27	36.2545400005486	38.0	35.0	40.0	31.0	41.0
28	36.06080252498092	38.0	35.0	40.0	30.0	41.0
29	35.91677070402063	37.0	35.0	40.0	30.0	41.0
30	35.77852714311707	37.0	34.0	40.0	30.0	41.0
31	35.65752782081735	38.0	34.0	40.0	29.0	41.0
32	35.58689524434581	38.0	35.0	40.0	29.0	41.0
33	35.24942667260231	37.0	34.0	40.0	26.0	41.0
34	35.17450090381009	37.0	34.0	40.0	26.0	41.0
35	35.09539594129752	37.0	34.0	40.0	26.0	41.0
36	35.022154986256	37.0	34.0	40.0	26.0	41.0
37	34.911393644213135	37.0	34.0	40.0	25.0	41.0
38	34.80555538079685	37.0	34.0	40.0	25.0	41.0
39	34.6707174337078	36.0	34.0	40.0	24.0	41.0
40	34.58221727529253	36.0	34.0	40.0	24.0	41.0
41	34.473456319284665	36.0	34.0	40.0	24.0	41.0
42	34.34866434156497	36.0	33.0	40.0	23.0	41.0
43	34.18295944896147	35.0	33.0	40.0	23.0	41.0
44	33.95532588626362	35.0	33.0	40.0	23.0	41.0
45	33.73319646790562	35.0	33.0	40.0	22.0	41.0
46	33.72765080089679	35.0	33.0	40.0	23.0	41.0
47	33.69513376968206	35.0	33.0	39.0	23.0	41.0
48	33.604653860740015	35.0	33.0	39.0	23.0	41.0
49	33.55328100805968	35.0	33.0	39.0	23.0	41.0
50	33.49272787074521	35.0	33.0	39.0	23.0	41.0
51	33.06897896763572	35.0	33.0	39.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	4.0
10	7.0
11	14.0
12	23.0
13	62.0
14	106.0
15	180.0
16	450.0
17	988.0
18	2009.0
19	3630.0
20	6246.0
21	10133.0
22	15314.0
23	23431.0
24	35251.0
25	52241.0
26	69320.0
27	81948.0
28	88565.0
29	96095.0
30	108429.0
31	127454.0
32	154715.0
33	208344.0
34	390636.0
35	395955.0
36	385560.0
37	444535.0
38	556067.0
39	715603.0
40	417.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.01326460534716	17.463931270671683	24.668743471189433	27.854060652791716
2	30.187206840519988	21.13438421756067	27.40000397611012	21.278404965809226
3	27.179380194894826	21.67674577028703	31.442500037118748	19.701373997699392
4	24.186602371120554	24.80380538903847	28.162486004973157	22.847106234867816
5	22.32656295729985	34.258517117279894	26.30055919710761	17.114360728312647
6	84.62850926320414	2.01754873817642	12.09807000118025	1.2558719974391837
7	80.26782373148875	7.140665968246986	11.226697918556681	1.3648123817075781
8	79.9894960230091	3.290759595574237	13.819750848886928	2.8999935325297397
9	46.015824414977054	17.983618929605992	21.08830663761254	14.912250017804418
10	31.294754831288362	18.54012335504172	27.384854493243505	22.780267320426407
11	27.781458895200057	15.01268454624405	36.203841576673625	21.002014981882276
12	23.446165104701297	17.011057360924852	36.5259064964858	23.016871037888052
13	19.45092939057556	21.324407049995557	39.288447412043034	19.936216147385846
14	20.03428514195594	18.71965227658728	40.2594235697265	20.98663901173028
15	25.34052992488423	15.404683706731175	38.45157689255922	20.803209475825376
16	24.361047911372	16.08902762213767	34.82002942824794	24.72989503824238
17	24.065884648012332	20.220508021047213	34.828736606108166	20.88487072483229
18	25.766728665463933	23.85369122686401	30.1708745907186	20.208705516953454
19	27.804409606785356	21.631624469988296	29.032473998630508	21.531491924595837
20	27.074717903794742	23.18014320539402	32.38753081799909	17.357608072812138
21	23.861895099645597	18.86568624515034	32.98681617511796	24.285602480086105
22	24.96956891668363	20.591519359755676	32.223830841176294	22.2150808823844
23	26.351594332080186	19.5420276098067	33.12250722431527	20.98387083379784
24	28.605167986877827	17.36206232275797	30.25152922956827	23.78124046079593
25	21.429773968205716	19.488576610456718	31.870007370902876	27.21164205043469
26	19.52426094053123	20.386623862247415	33.877766825300036	26.211348371921318
27	21.087576845248535	22.936241564292317	32.84455699464458	23.131624595814564
28	24.836620880164823	23.180218701155813	30.589876068673966	21.3932843500054
29	23.28475516598624	20.06506224751386	35.751345145735755	20.898837440764137
30	28.486941623908802	20.99569850314553	30.28046927158921	20.236890601356457
31	29.769539121022977	20.98991049474134	28.884804288561917	20.355746095673766
32	30.114001116833972	19.725381649949806	27.551876283585237	22.60874094963099
33	23.622296716966137	22.525091645563506	32.16212563853686	21.690485998933497
34	22.84413673490393	24.2586253278718	30.907990043618934	21.98924789360533
35	26.516124762282722	27.2341146221953	26.605939553563363	19.643821061958615
36	25.488124139191033	25.575548231348204	26.74163060276068	22.194697026700084
37	25.262618298713075	29.722882740234436	24.888108989708165	20.126389971344324
38	25.131431829969454	24.329188699895035	25.26113354873113	25.27824592140438
39	23.50162932436578	21.415907913289594	31.337812580764734	23.74465018157989
40	21.345571028551742	22.90939023834767	31.21052672638046	24.53451200672013
41	21.75639379897945	26.580900125901763	25.87949416832988	25.783211906788907
42	20.44276754376804	21.120316840613096	28.59993361406013	29.836982001558738
43	21.5489314455702	20.076462107544717	31.972580945926666	26.40202550095842
44	25.262039497872657	22.75613384190634	29.283371580325102	22.698455079895908
45	25.43057120345026	23.90527999742308	28.804300641235837	21.85984815789083
46	22.504053493276977	25.26412821394895	27.9422145373129	24.289603755461176
47	21.209930309862287	26.601510468871464	30.336084482777277	21.852474738488972
48	24.626415514077067	24.66247732296055	29.60669476283384	21.104412400128545
49	22.307236042280646	22.45910834975576	31.76182194425242	23.471833663711177
50	21.094144976524593	23.186661006162215	33.41069971233598	22.30849430497721
51	20.55502974155536	20.592500804658993	32.526644341731064	26.325825112054584
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1231.0
1	13902.0
2	26573.0
3	96079.5
4	165586.0
5	101029.0
6	36472.0
7	32565.5
8	28659.0
9	28090.0
10	27521.0
11	27493.0
12	27465.0
13	26554.5
14	25644.0
15	23853.0
16	22062.0
17	20765.0
18	19468.0
19	18392.5
20	17317.0
21	16514.5
22	15712.0
23	15687.0
24	15662.0
25	17090.0
26	19907.0
27	21296.0
28	24147.5
29	26999.0
30	30651.0
31	34303.0
32	38902.0
33	43501.0
34	53244.5
35	62988.0
36	69102.5
37	75217.0
38	79604.5
39	83992.0
40	93985.0
41	103978.0
42	118928.0
43	133878.0
44	148809.5
45	163741.0
46	181866.0
47	199991.0
48	221818.0
49	243645.0
50	234684.0
51	225723.0
52	202814.5
53	179906.0
54	188514.5
55	197123.0
56	217804.0
57	238485.0
58	249877.0
59	261269.0
60	265705.0
61	270141.0
62	267229.0
63	264317.0
64	243135.5
65	221954.0
66	187581.0
67	153208.0
68	124487.0
69	95766.0
70	83736.5
71	71707.0
72	68294.5
73	64882.0
74	54467.0
75	33069.0
76	22086.0
77	16496.0
78	10906.0
79	8244.5
80	5583.0
81	4209.0
82	2835.0
83	2164.0
84	1493.0
85	1008.0
86	523.0
87	385.5
88	248.0
89	153.0
90	58.0
91	41.5
92	25.0
93	19.0
94	13.0
95	12.0
96	11.0
97	5.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3973733.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.421907130519468
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.97712175579052	17.259738751830252
2	9.23731812478153	4.142365782578314
3	2.9638651900836246	1.9936653011830336
4	1.3948235726415352	1.250984184369115
5	0.8564227247835583	0.9601315399781691
6	0.60368361889193	0.8121442823406457
7	0.4699779659563333	0.7376461614244343
8	0.3889629550496192	0.6977033004267984
9	0.3306133804821683	0.6671684261950448
>10	4.830741310776432	26.845391498543602
>50	1.5295577648805818	23.818904194989344
>100	0.40688122903144053	13.089760725235935
>500	0.005438172388929855	0.8516968126623817
>1k	0.003625448259286457	1.4167669746393563
>5k	3.6254482592864564E-4	0.4786594615576681
>10k+	6.042413765477428E-4	4.977272602046044
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	104293	2.624559828252175	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	40686	1.0238735214469619	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	15708	0.395295808752123	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	12001	0.3020082124289679	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10999	0.2767926279898524	No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	6953	0.1749740105840025	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	5531	0.13918901949376064	No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	5181	0.13038118061782208	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4370	0.10997215967957585	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.02863805897376598	0.0
2	0.0	0.0	0.0	0.1000822148845934	0.0
3	0.0	0.0	0.0	0.16274369717341353	0.0
4	0.0	0.0	0.0	0.20454318395322485	0.0
5	0.0	0.0	0.0	0.46080096473517473	0.0
6	0.0	0.0	0.0	0.5592474381142367	0.0
7	0.0	0.0	0.0	0.8375499813399642	0.0
8	0.0	0.0	0.0	1.1498004521189522	0.0
9	0.0	0.0	0.0	1.4351492664454306	0.0
10	0.0	0.0	0.0	1.6194092557300654	0.0
11	0.0	0.0	0.0	1.783839024916873	0.0
12	0.0	0.0	0.0	1.9132639258853072	0.0
13	0.0	0.0	0.0	1.9830723402906034	0.0
14	0.0	0.0	0.0	2.0359948693080283	0.0
15	0.0	0.0	0.0	2.181374541268877	0.0
16	0.0	0.0	0.0	2.3447473697905723	0.0
17	0.0	0.0	0.0	2.572165769567306	0.0
18	0.0	0.0	0.0	2.696230471448384	0.0
19	0.0	0.0	0.0	2.8381373383667197	0.0
20	0.0	0.0	0.0	3.0103180057643533	0.0
21	0.0	0.0	0.0	3.2012971178486325	0.0
22	2.5165253931253056E-5	0.0	0.0	3.3760194758933224	0.0
23	2.5165253931253056E-5	0.0	0.0	3.5477723339741245	0.0
24	5.033050786250611E-5	0.0	0.0	3.679965412874997	0.0
25	5.033050786250611E-5	0.0	0.0	3.8173425340857072	0.0
26	5.033050786250611E-5	0.0	0.0	3.9521527993954297	0.0
27	5.033050786250611E-5	0.0	0.0	4.11041708136908	0.0
28	5.033050786250611E-5	0.0	0.0	4.267171448106856	0.0
29	5.033050786250611E-5	0.0	0.0	4.427851594457906	0.0
30	5.033050786250611E-5	0.0	0.0	4.592910494992995	0.0
31	5.033050786250611E-5	0.0	0.0	4.768865950480317	0.0
32	5.033050786250611E-5	0.0	0.0	4.9763283038895665	0.0
33	5.033050786250611E-5	0.0	0.0	5.172692780315135	0.0
34	5.033050786250611E-5	0.0	0.0	5.400287336869387	0.0
35	5.033050786250611E-5	0.0	0.0	5.5997974700363615	0.0
36	5.033050786250611E-5	0.0	0.0	5.827769505399583	0.0
37	5.033050786250611E-5	0.0	0.0	6.121397688269443	0.0
38	1.5099152358751834E-4	0.0	0.0	6.442632154701888	0.0
39	1.5099152358751834E-4	0.0	0.0	6.81198258665089	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACACG	55	1.8189894E-12	45.0	19
CGTTTTT	30930	0.0	43.67604	1
CGGTCTA	1015	0.0	41.89655	30
GCACTTA	11625	0.0	41.690323	35
CACTTAC	11630	0.0	41.65305	36
CGCACTT	11695	0.0	41.594696	34
ACTTACT	11640	0.0	41.50129	37
CCCGCAC	11825	0.0	41.498943	32
CTTACTG	11670	0.0	41.491	38
CCGCACT	11755	0.0	41.40153	33
ACCCGCA	11785	0.0	41.37251	31
TATACGG	230	0.0	41.086956	1
TCACGAC	1085	0.0	40.230415	24
TTATGAC	12410	0.0	40.177277	26
TATGACC	12350	0.0	40.172066	27
GTAGGGT	1490	0.0	40.167786	3
TCAACGC	12715	0.0	40.116005	15
AACGCAA	12665	0.0	40.11449	17
GCTTATG	12540	0.0	40.029903	24
CAACGCA	12715	0.0	40.027527	16
>>END_MODULE