##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043435_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1114788
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.53451597971991	31.0	31.0	33.0	30.0	34.0
2	30.552448537300364	31.0	31.0	34.0	26.0	34.0
3	31.490058199406523	31.0	31.0	34.0	28.0	34.0
4	35.08932998919974	37.0	35.0	37.0	32.0	37.0
5	35.410741773323714	37.0	35.0	37.0	33.0	37.0
6	35.8329009641295	37.0	35.0	37.0	35.0	37.0
7	33.740645755067334	37.0	35.0	37.0	32.0	37.0
8	34.78094938230408	37.0	35.0	37.0	31.0	37.0
9	36.71512610469435	39.0	37.0	39.0	32.0	39.0
10	36.67786700251528	39.0	35.0	39.0	32.0	39.0
11	36.3732575162273	38.0	35.0	39.0	32.0	39.0
12	36.25644427460647	39.0	35.0	39.0	32.0	39.0
13	36.28149477748236	39.0	35.0	39.0	32.0	39.0
14	37.6285607667108	40.0	35.0	41.0	33.0	41.0
15	37.81804163661611	40.0	35.0	41.0	33.0	41.0
16	37.78784755487142	40.0	35.0	41.0	33.0	41.0
17	37.72954229862539	40.0	36.0	41.0	33.0	41.0
18	37.44560669831394	39.0	36.0	41.0	33.0	41.0
19	37.16939543662113	38.0	35.0	41.0	33.0	41.0
20	36.87569205983559	38.0	35.0	40.0	32.0	41.0
21	36.77126323570042	38.0	35.0	40.0	32.0	41.0
22	36.707463661252184	38.0	35.0	40.0	32.0	41.0
23	36.614288994858214	37.0	35.0	40.0	32.0	41.0
24	36.47819854537365	37.0	35.0	40.0	32.0	41.0
25	36.40465003211373	37.0	35.0	40.0	32.0	41.0
26	36.14680369720521	37.0	35.0	40.0	31.0	41.0
27	36.08814231943652	37.0	35.0	40.0	31.0	41.0
28	35.98737158993459	36.0	35.0	40.0	31.0	41.0
29	35.910539941226496	36.0	35.0	40.0	31.0	41.0
30	35.73514426061278	36.0	35.0	40.0	30.0	41.0
31	35.30781188889726	36.0	34.0	40.0	29.0	41.0
32	35.122818867802664	37.0	34.0	40.0	27.0	41.0
33	34.96734715479535	37.0	34.0	40.0	25.0	41.0
34	34.73867138864071	37.0	34.0	40.0	24.0	41.0
35	34.49675184878201	37.0	34.0	40.0	23.0	41.0
36	34.31573895664467	37.0	34.0	40.0	22.0	41.0
37	34.24726136269856	36.0	34.0	40.0	21.0	41.0
38	34.156834303921464	36.0	34.0	40.0	21.0	41.0
39	34.01685881082322	36.0	34.0	40.0	20.0	41.0
40	33.968978855172466	36.0	34.0	40.0	20.0	41.0
41	33.9093424041163	36.0	33.0	40.0	20.0	41.0
42	33.80494138795897	36.0	33.0	40.0	18.0	41.0
43	33.654210486657554	35.0	33.0	40.0	18.0	41.0
44	33.46337509912198	35.0	33.0	40.0	18.0	41.0
45	33.294342063244315	35.0	33.0	40.0	18.0	41.0
46	33.312182226575814	35.0	33.0	40.0	18.0	41.0
47	33.291700305349536	35.0	33.0	40.0	18.0	41.0
48	33.24087988030011	35.0	33.0	40.0	18.0	41.0
49	33.14289981592913	35.0	33.0	40.0	17.0	41.0
50	33.06553263938973	35.0	33.0	40.0	17.0	41.0
51	32.592503686799645	35.0	32.0	39.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	4.0
11	4.0
12	8.0
13	7.0
14	30.0
15	72.0
16	122.0
17	324.0
18	672.0
19	1122.0
20	2037.0
21	3314.0
22	5290.0
23	8520.0
24	13654.0
25	20676.0
26	25484.0
27	27260.0
28	26754.0
29	26931.0
30	29164.0
31	34223.0
32	42344.0
33	56326.0
34	97606.0
35	133748.0
36	104396.0
37	100807.0
38	135931.0
39	217847.0
40	110.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.34097245395537	18.021004890615973	25.620387015289005	30.017635640139652
2	31.60188304861552	21.021485699523137	29.22753025687395	18.14910099498739
3	21.767188021399583	21.71632633289917	38.44748956752315	18.068996078178092
4	22.678751475616888	24.79117105673904	35.31667007538653	17.21340739225754
5	19.369422706380046	31.533080729250763	33.08422767378192	16.013268890587266
6	79.00461791838448	1.988898337621144	17.711080492434437	1.2954032515599379
7	75.49516141185589	7.229715425713231	15.840680021672283	1.4344431407586016
8	74.99677068644442	3.499499456398885	18.830396452060842	2.673333405095857
9	40.111213970728066	21.802800173665307	26.299798706121702	11.786187149484924
10	31.34766430926777	23.68674582073004	34.49785968273788	10.467730187264305
11	29.39742803115929	20.764127349774128	36.73990032185492	13.098544297211667
12	19.52550619489984	30.298316810012306	35.68158250716728	14.494594487920573
13	14.367216008783734	32.51299798706121	35.907903565520975	17.21188243863407
14	13.02561563274811	23.85484953192894	45.695594139872334	17.423940695450614
15	15.513532617860976	20.936716218689114	46.05342002246167	17.496331140988243
16	17.27180414572098	22.069218542000808	35.9183091314223	24.740668180855913
17	18.831293483604057	22.328281251681933	40.83422139456112	18.00620387015289
18	22.428120862442007	24.136517436499137	35.612421375185235	17.822940325873617
19	22.856004908556603	24.18092049788839	35.72966339788372	17.233411195671284
20	18.00351277552324	26.779172362816965	37.04013677936971	18.177178082290084
21	18.84241667473995	24.232410108469054	34.915248459796835	22.009924756994153
22	15.025816567813791	27.400097597031902	36.093050876041005	21.481034959113302
23	14.837798756355468	23.75518932747751	45.63459599493356	15.772415921233454
24	15.823187906579546	24.09955973691859	42.12612622310251	17.951126133399356
25	15.272948757970125	29.139352056175703	36.70339113804598	18.884308047808194
26	14.968765361665179	30.42165864720467	36.30143130353036	18.308144687599796
27	15.252496438784775	27.712264574071483	40.37870877691543	16.65653021022831
28	14.049487436176205	24.479721704934033	40.26397844253795	21.20681241635181
29	15.495143471225022	29.26027190820138	38.268442071497	16.976142549076595
30	20.58768124522331	27.6065942582805	35.76321237760005	16.04251211889615
31	21.112265291696716	26.422871433851096	35.46728167149269	16.997581602959485
32	19.057255729340465	28.343954186805025	35.77971775799524	16.819072325859267
33	17.163980954226275	26.858469951237364	35.512581764425164	20.464967330111197
34	16.828939672834657	26.087561042996516	36.49474160109366	20.58875768307517
35	22.782448322012797	26.16040000430575	34.38294994205176	16.67420173162969
36	17.432911010882787	29.566608180210046	35.68400449233397	17.316476316573194
37	17.690000251168833	28.962188326390308	35.12291126205162	18.22490016038924
38	17.055978356422923	30.423632116599748	34.624879349257434	17.89551017771989
39	20.2700423757701	26.459111508197076	33.471117378371495	19.799728737661333
40	15.33008966727306	26.650627742673944	35.7650961438408	22.254186446212195
41	17.80410266346606	26.94628933931833	33.42061450248836	21.828993494727246
42	17.87245646705921	25.83621280458706	35.21207619744741	21.079254530906326
43	17.732609249471647	27.758820511164455	34.73144669659164	19.77712354277226
44	15.307753581846953	33.33719056896916	32.17688026781774	19.178175581366144
45	18.626590885441896	29.796696771045255	33.13508936228233	18.441622981230513
46	17.459821957179305	29.102394356595156	32.54959687402448	20.888186812201067
47	17.026466018651078	27.183285073036306	36.62310681492804	19.16714209338457
48	18.102455354740094	25.383391281571026	36.68042713053962	19.833726233149264
49	17.502879471253728	29.19667237178728	33.69860457773137	19.60184357922762
50	16.180116757625665	28.283314854483542	34.20991255736517	21.326655830525628
51	16.450840877368613	26.258445551979392	35.87543102365652	21.41528254699548
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1010.0
1	5773.5
2	10537.0
3	37932.0
4	65327.0
5	39989.0
6	14651.0
7	14324.0
8	13997.0
9	15300.5
10	16604.0
11	17166.5
12	17729.0
13	17490.5
14	17252.0
15	15573.5
16	13895.0
17	12372.0
18	10849.0
19	9860.5
20	8872.0
21	7944.5
22	7017.0
23	6566.5
24	6116.0
25	6876.0
26	8111.5
27	8587.0
28	9814.5
29	11042.0
30	14410.5
31	17779.0
32	20816.5
33	23854.0
34	26010.0
35	28166.0
36	32171.5
37	36177.0
38	41005.5
39	45834.0
40	54931.5
41	64029.0
42	73666.0
43	83303.0
44	91587.5
45	99872.0
46	97612.5
47	95353.0
48	91773.0
49	88193.0
50	79262.5
51	70332.0
52	63134.0
53	55936.0
54	49791.0
55	43646.0
56	40571.0
57	37496.0
58	33922.5
59	30349.0
60	24815.0
61	19281.0
62	16397.0
63	13513.0
64	11345.5
65	9178.0
66	7636.5
67	6095.0
68	5326.0
69	4557.0
70	3876.0
71	3195.0
72	3113.5
73	3032.0
74	2511.5
75	1558.5
76	1126.0
77	948.5
78	771.0
79	553.5
80	336.0
81	232.0
82	128.0
83	99.5
84	71.0
85	56.0
86	41.0
87	26.0
88	11.0
89	8.5
90	6.0
91	5.5
92	5.0
93	6.0
94	7.0
95	5.0
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1114788.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.492295612205965
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.10049580569726	25.701566927907738
2	9.556999566163912	6.21057710139044
3	3.461433762154092	3.3740978712594294
4	1.7370762986019264	2.257663863805214
5	0.9973814006817074	1.6203605654533038
6	0.6833808399943648	1.3322767361288714
7	0.4703571453401184	1.0698088386793116
8	0.3563532095528283	0.92629870617191
9	0.29201433161120977	0.8539394387140952
>10	2.29545912713183	16.696336491497743
>50	0.6742658028734102	15.778076924176457
>100	0.3681975288875462	17.525346613267693
>500	0.004867307924622756	1.0558838764235852
>1k	0.0011452489234406486	0.5274767911296676
>5k	2.8631223086016214E-4	0.4989167602871971
>10k+	2.8631223086016214E-4	4.571372493707361
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	49139	4.407923300214929	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5363	0.4810780166273767	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2111	0.1893633587731479	No Hit
GTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATT	1395	0.12513590027879742	No Hit
GCGAAGGGTCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCACAAGCCA	1158	0.10387625270455009	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.08333423036487655	0.0
2	0.0	0.0	0.0	0.28920296953322067	0.0
3	0.0	0.0	0.0	0.4626888699914244	0.0
4	0.0	0.0	0.0	0.5851336756405702	0.0
5	0.0	0.0	0.0	1.298901674578485	0.0
6	0.0	0.0	0.0	1.4517558495426932	0.0
7	0.0	0.0	0.0	1.9788515843371117	0.0
8	0.0	0.0	0.0	2.596726911305109	0.0
9	0.0	0.0	0.0	3.0485616996236056	0.0
10	0.0	0.0	0.0	3.4084507547623404	0.0
11	0.0	0.0	0.0	3.8145369343767603	0.0
12	0.0	0.0	0.0	4.146528308521441	0.0
13	0.0	0.0	0.0	4.2967810920103195	0.0
14	0.0	0.0	0.0	4.416624506184135	0.0
15	0.0	0.0	0.0	4.8628976989346855	0.0
16	0.0	0.0	0.0	5.398246123926702	0.0
17	0.0	0.0	0.0	6.018453732907064	0.0
18	0.0	0.0	0.0	6.359953641409846	0.0
19	0.0	0.0	0.0	6.725673401579493	0.0
20	0.0	0.0	0.0	7.167371733459635	0.0
21	0.0	0.0	0.0	7.605751048629874	0.0
22	0.0	0.0	0.0	8.05650939909651	0.0
23	8.970315432171856E-5	0.0	0.0	8.410208936587047	0.0
24	0.0011661410061823414	0.0	0.0	8.716096692824106	0.0
25	0.0011661410061823414	0.0	0.0	9.029250404561227	0.0
26	0.0011661410061823414	0.0	0.0	9.374786955008487	0.0
27	0.001435250469147497	0.0	0.0	9.697269794795064	0.0
28	0.0016146567777909342	0.0	0.0	10.043434267322576	0.0
29	0.0016146567777909342	0.0	0.0	10.436782599023312	0.0
30	0.0016146567777909342	0.0	0.0	10.842420262866124	0.0
31	0.0016146567777909342	0.0	0.0	11.196030097202337	0.0
32	0.0016146567777909342	0.0	0.0	11.571527501193051	0.0
33	0.0016146567777909342	0.0	0.0	11.950074812430705	0.0
34	0.0016146567777909342	0.0	0.0	12.385673329816969	0.0
35	0.0016146567777909342	0.0	0.0	12.768885205079352	0.0
36	0.0016146567777909342	0.0	0.0	13.132990308471207	0.0
37	0.0016146567777909342	0.0	0.0	13.539704410165879	0.0
38	0.0016146567777909342	0.0	0.0	13.95574763991001	0.0
39	0.0016146567777909342	0.0	0.0	14.380312669314703	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCATCGA	65	0.0	45.000004	15
AACCGTA	20	7.0331956E-4	45.0	37
CTAGACG	20	7.0331956E-4	45.0	12
GGTACGC	20	7.0331956E-4	45.0	7
CTCGTAG	25	3.8907303E-5	45.0	21
TCGATCC	20	7.0331956E-4	45.0	45
GTTACGC	25	3.8907303E-5	45.0	9
CTAACGG	80	0.0	45.0	1
CCACTCG	20	7.0331956E-4	45.0	18
TACGCCC	20	7.0331956E-4	45.0	37
CTAGTTC	25	3.8907303E-5	45.0	32
GTAGACT	25	3.8907303E-5	45.0	8
CACTAGT	25	3.8907303E-5	45.0	29
GTATGCG	20	7.0331956E-4	45.0	11
CCGAGTG	25	3.8907303E-5	45.0	13
AGCGCGC	20	7.0331956E-4	45.0	35
ACGTGAC	20	7.0331956E-4	45.0	28
GTCGTGG	35	1.2118835E-7	45.0	1
TCCACCG	25	3.8907303E-5	45.0	30
CGTGTAT	25	3.8907303E-5	45.0	21
>>END_MODULE