##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043434_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	458946
Sequences flagged as poor quality	0
Sequence length	51
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.529661441651086	31.0	31.0	33.0	30.0	34.0
2	30.564659023065893	31.0	31.0	34.0	26.0	34.0
3	31.47395336270498	31.0	31.0	34.0	28.0	34.0
4	35.0795779895674	37.0	35.0	37.0	32.0	37.0
5	35.38560963599203	37.0	35.0	37.0	33.0	37.0
6	35.81251824833423	37.0	35.0	37.0	35.0	37.0
7	33.717881842308245	37.0	35.0	37.0	32.0	37.0
8	34.76371512116894	37.0	35.0	37.0	31.0	37.0
9	36.766183821190296	39.0	37.0	39.0	32.0	39.0
10	36.70637068413277	39.0	35.0	39.0	32.0	39.0
11	36.18309779364021	37.0	35.0	39.0	32.0	39.0
12	35.870052250155794	38.0	35.0	39.0	31.0	39.0
13	35.96363406588139	38.0	35.0	39.0	31.0	39.0
14	37.35298270384751	39.0	35.0	41.0	32.0	41.0
15	37.57638807179929	40.0	35.0	41.0	33.0	41.0
16	37.548193033603084	40.0	35.0	41.0	33.0	41.0
17	37.54458912377491	39.0	35.0	41.0	33.0	41.0
18	37.26384803440928	39.0	35.0	40.0	33.0	41.0
19	36.96175367036645	37.0	35.0	40.0	33.0	41.0
20	36.62779063332069	37.0	35.0	40.0	32.0	41.0
21	36.514550731458606	37.0	35.0	40.0	32.0	41.0
22	36.456513402448216	37.0	35.0	40.0	32.0	41.0
23	36.35641883794607	36.0	35.0	40.0	32.0	41.0
24	36.219095492715915	36.0	35.0	40.0	32.0	41.0
25	36.113226828428616	35.0	35.0	40.0	32.0	41.0
26	35.857737075821554	35.0	35.0	40.0	31.0	41.0
27	35.78262148488057	36.0	35.0	40.0	31.0	41.0
28	35.726261913166255	36.0	35.0	40.0	31.0	41.0
29	35.67923677295368	36.0	35.0	40.0	31.0	41.0
30	35.50950874394809	35.0	35.0	40.0	31.0	41.0
31	35.05631163579157	35.0	34.0	40.0	29.0	41.0
32	34.89801414545502	35.0	34.0	40.0	27.0	41.0
33	34.784375503871914	35.0	34.0	40.0	27.0	41.0
34	34.61173863591795	35.0	34.0	40.0	25.0	41.0
35	34.38031053762316	35.0	34.0	40.0	23.0	41.0
36	34.233227874303296	35.0	34.0	40.0	23.0	41.0
37	34.176748462782115	35.0	34.0	40.0	23.0	41.0
38	34.09398273435219	35.0	34.0	40.0	23.0	41.0
39	33.94338767523848	35.0	33.0	40.0	22.0	41.0
40	33.910109250325746	35.0	34.0	40.0	22.0	41.0
41	33.84737637979196	35.0	33.0	40.0	22.0	41.0
42	33.743261734495995	35.0	33.0	40.0	21.0	41.0
43	33.58621493596196	35.0	33.0	40.0	21.0	41.0
44	33.3830232750694	35.0	33.0	40.0	20.0	41.0
45	33.2217929778231	35.0	33.0	40.0	19.0	41.0
46	33.24529465340149	35.0	33.0	40.0	20.0	41.0
47	33.25783861282155	35.0	33.0	39.0	20.0	41.0
48	33.21476164951868	35.0	33.0	39.0	20.0	41.0
49	33.09203915057545	35.0	33.0	39.0	19.0	41.0
50	32.968384080044274	35.0	33.0	39.0	18.0	41.0
51	32.553995459160774	35.0	32.0	39.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	1.0
11	0.0
12	1.0
13	5.0
14	4.0
15	17.0
16	54.0
17	131.0
18	268.0
19	527.0
20	866.0
21	1431.0
22	2108.0
23	3266.0
24	5223.0
25	7634.0
26	9816.0
27	10406.0
28	10569.0
29	11093.0
30	12305.0
31	14808.0
32	18313.0
33	24852.0
34	46449.0
35	67777.0
36	47281.0
37	37691.0
38	48908.0
39	77108.0
40	33.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.00119839806862	18.836202951981278	25.588195561133553	30.57440308881655
2	31.287340994365348	21.347173741573084	29.175327816344403	18.190157447717162
3	21.269822593507733	21.82958343683135	38.32585968719632	18.574734282464604
4	21.90083364927464	25.349169619083728	35.61835161435115	17.131645117290486
5	19.015308990600204	31.20781094072069	33.13941073677513	16.63746933190397
6	78.66350289576552	2.0845589677216925	17.81778248421383	1.4341556522989634
7	75.18793060621512	7.306306188527627	15.986630235365382	1.5191329698918827
8	75.00947823926998	3.4531295620835567	19.078497252400066	2.4588949462463994
9	43.318603931617226	22.15489403982168	24.87089984442614	9.655602184134953
10	35.47781220448593	21.38116466861025	33.46341399641788	9.677609130485939
11	32.37940847071333	21.022516810256544	34.62215598349261	11.975918735537515
12	17.804491160180064	36.6725061336192	33.013252103733336	12.509750602467392
13	11.456249754873122	39.54386790602816	34.696020882631075	14.303861456467645
14	10.271143010288792	24.17365005904834	51.84030365228153	13.714903278381335
15	11.818601752711649	21.28442126088908	50.544290613710544	16.352686372688726
16	13.611405263364318	21.56244961280848	35.56104639761541	29.265098726211797
17	16.182295956387026	22.378885533374294	42.31216744453596	19.126651065702717
18	20.80789461069494	25.305373616939686	35.886357000605734	18.000374771759642
19	23.18224802046428	24.160140844456645	35.74538180962466	16.912229325454412
20	15.783556235374096	29.585179955811796	37.25514548552553	17.376118323288576
21	17.036644834032764	25.56706017701429	33.42898728826485	23.9673077006881
22	12.906093527343085	30.34561800298946	34.27570999638302	22.47257847328444
23	12.4435118728565	24.412022329424378	48.05467309879594	15.089792698923185
24	13.045543484418646	25.244800041834985	44.920753204080654	16.78890326966571
25	11.856514709791565	33.006715386995424	36.93615370871519	18.20061619449783
26	12.88844439215071	35.51441781821826	35.129623092912894	16.467514696718133
27	12.16395828703159	29.58278315967456	42.185790920936235	16.06746763235762
28	10.68883920984168	25.999355043948526	40.96320700038785	22.34859874582195
29	12.380323611056639	33.33006497496437	37.54951562928972	16.740095784689267
30	20.92817019867261	29.264445054538008	32.99603874965682	16.81134599713256
31	20.76562384245641	29.11410056956592	34.39598558436069	15.724290003616984
32	19.442156593586173	30.02836935064256	33.28779420672585	17.241679849045422
33	14.322817935007603	30.855046127431113	32.67072814666649	22.15140779089479
34	13.879846430734771	28.84260893438444	35.86500372592854	21.412540908952252
35	22.901604981849715	27.986516932275258	31.200402661751053	17.91147542412397
36	15.90492127614142	32.840900672410264	35.011090629398666	16.243087422049655
37	16.00122890274673	32.72411133335948	31.9279392346812	19.34672052921259
38	15.317706222518554	32.623663786153486	33.63554753718302	18.423082454144932
39	19.453269012040632	28.74259716829431	31.998317884892774	19.805815934772284
40	12.973857490859492	27.013635591115293	35.4198533160764	24.592653601948815
41	16.32370692848396	28.458903661868717	31.579532232550235	23.63785717709709
42	16.130002222483693	26.89902515764382	32.87707050502674	24.093902114845754
43	16.065724507894174	30.328622539470874	33.63118972602442	19.97446322661054
44	12.46639038143921	38.37357771938311	29.894802438631125	19.265229460546557
45	17.049936158066526	33.66953846422019	31.010184204677678	18.270341173035607
46	15.592684106626923	32.11118519390081	31.101044567334718	21.19508613213755
47	15.93106814309309	28.873767284168505	35.49785813581554	19.69730643692286
48	17.673974715979657	25.614778209201084	36.17745878600097	20.533788288818293
49	17.285475851189464	31.916608925668815	32.144740339822114	18.653174883319608
50	14.165065171065876	31.527020608088968	31.99396007373416	22.31395414711099
51	14.641809711817949	28.563491129675388	35.95281362077456	20.841885537732107
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	620.0
1	2752.5
2	4885.0
3	18709.5
4	32534.0
5	18884.0
6	5234.0
7	5362.0
8	5490.0
9	6478.0
10	7466.0
11	8372.0
12	9278.0
13	9077.5
14	8877.0
15	7793.0
16	6709.0
17	5844.0
18	4979.0
19	4423.5
20	3868.0
21	3544.5
22	3221.0
23	3010.5
24	2800.0
25	2907.0
26	3228.5
27	3443.0
28	3734.0
29	4025.0
30	5052.0
31	6079.0
32	6898.0
33	7717.0
34	9748.5
35	11780.0
36	13241.5
37	14703.0
38	15815.5
39	16928.0
40	21232.0
41	25536.0
42	33675.5
43	41815.0
44	44361.5
45	46908.0
46	44850.0
47	42792.0
48	38409.5
49	34027.0
50	29214.0
51	24401.0
52	21127.0
53	17853.0
54	16092.0
55	14331.0
56	13999.0
57	13667.0
58	12616.5
59	11566.0
60	9221.0
61	6876.0
62	5561.5
63	4247.0
64	3631.0
65	3015.0
66	2636.0
67	2257.0
68	1906.5
69	1556.0
70	1420.5
71	1285.0
72	1366.5
73	1448.0
74	1193.0
75	698.5
76	459.0
77	303.5
78	148.0
79	132.5
80	117.0
81	73.5
82	30.0
83	21.0
84	12.0
85	10.0
86	8.0
87	5.0
88	2.0
89	2.0
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	458946.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.5049104798567
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.94397425511515	27.2395476459686
2	9.300542751306699	6.418287900958355
3	3.4221018377543135	3.54237952693997
4	1.7684715535515865	2.4408381056588238
5	1.1329226594587043	1.9545697472611885
6	0.7536439375455405	1.5602649959217352
7	0.49011165122308253	1.1837881055411048
8	0.3668295764366932	1.012593735704948
9	0.3091962325401541	0.9601909492056274
>10	2.5984928494207264	20.691315987502502
>50	0.7461349836842095	17.741626035203296
>100	0.16439182390309653	8.140666045472793
>500	0.001274355224055012	0.3209920443565581
>1k	0.001274355224055012	0.8827281219805349
>5k	0.0	0.0
>10k+	6.37177612027506E-4	5.9102110523239695
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	26882	5.857333978289385	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2885	0.6286142596296732	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1130	0.2462163304615358	No Hit
GTCATGGGATTGAAGGGAATAATTTAGATTTTGATGAGATTCAGGAAATTA	917	0.19980564162232592	No Hit
GCGAAGGGTCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCACAAGCCA	543	0.11831457295629552	No Hit
CCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC	500	0.10894527896528133	No Hit
CTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	475	0.10349801501701725	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.10415168669080895	0.0
2	0.0	0.0	0.0	0.356686843332331	0.0
3	0.0	0.0	0.0	0.5702195901042825	0.0
4	0.0	0.0	0.0	0.7188209506129262	0.0
5	0.0	0.0	0.0	1.5840643561551904	0.0
6	0.0	0.0	0.0	1.784523669451308	0.0
7	0.0	0.0	0.0	2.4647779913105246	0.0
8	0.0	0.0	0.0	3.2191151028661324	0.0
9	0.0	0.0	0.0	3.792820941897304	0.0
10	0.0	0.0	0.0	4.177615667202677	0.0
11	0.0	0.0	0.0	4.642376227268567	0.0
12	0.0	0.0	0.0	5.04242329162908	0.0
13	0.0	0.0	0.0	5.218043081321114	0.0
14	0.0	0.0	0.0	5.35574991393323	0.0
15	0.0	0.0	0.0	5.844478435371482	0.0
16	0.0	0.0	0.0	6.417094821613	0.0
17	0.0	0.0	0.0	7.228519259346416	0.0
18	0.0	0.0	0.0	7.535309164912648	0.0
19	0.0	0.0	0.0	7.924025920260771	0.0
20	0.0	0.0	0.0	8.326905561874382	0.0
21	2.1789055793056263E-4	0.0	0.0	8.680324046837754	0.0
22	2.1789055793056263E-4	0.0	0.0	9.070783926649323	0.0
23	2.1789055793056263E-4	0.0	0.0	9.373433911614875	0.0
24	0.0026146866951667516	0.0	0.0	9.63555625280534	0.0
25	0.0026146866951667516	0.0	0.0	9.882644145498599	0.0
26	0.0026146866951667516	0.0	0.0	10.153046327890427	0.0
27	0.0026146866951667516	0.0	0.0	10.409067733458839	0.0
28	0.0026146866951667516	0.0	0.0	10.682302493103764	0.0
29	0.0026146866951667516	0.0	0.0	11.057510033860193	0.0
30	0.0026146866951667516	0.0	0.0	11.411582190497358	0.0
31	0.0026146866951667516	0.0	0.0	11.688956870742963	0.0
32	0.0026146866951667516	0.0	0.0	11.965024207640987	0.0
33	0.0026146866951667516	0.0	0.0	12.244795684023828	0.0
34	0.0026146866951667516	0.0	0.0	12.606929791304424	0.0
35	0.0026146866951667516	0.0	0.0	12.903478840647919	0.0
36	0.0026146866951667516	0.0	0.0	13.208307731192777	0.0
37	0.0026146866951667516	0.0	0.0	13.518583885685898	0.0
38	0.0026146866951667516	0.0	0.0	13.858057374941714	0.0
39	0.0026146866951667516	0.0	0.0	14.188597351322377	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAGGTAA	20	7.0288515E-4	45.000004	18
GTCGCTG	20	7.0288515E-4	45.000004	11
AGTCCAT	20	7.0288515E-4	45.000004	21
ACGCCTA	20	7.0288515E-4	45.000004	17
ACTGCAC	20	7.0288515E-4	45.000004	37
GCGACAC	20	7.0288515E-4	45.000004	15
TCCGCCT	20	7.0288515E-4	45.000004	29
CTGTACG	20	7.0288515E-4	45.000004	12
TTGTGCG	20	7.0288515E-4	45.000004	26
CCGATTC	20	7.0288515E-4	45.000004	11
TAGACGC	20	7.0288515E-4	45.000004	9
TGGACGT	20	7.0288515E-4	45.000004	10
GGCACGG	20	7.0288515E-4	45.000004	1
TGGACCA	20	7.0288515E-4	45.000004	23
CTTCATC	25	3.8871294E-5	45.000004	14
GCAACTA	40	6.7993824E-9	45.000004	8
GCAACCT	20	7.0288515E-4	45.000004	23
CATGAGA	25	3.8871294E-5	45.000004	3
CAGTTCA	20	7.0288515E-4	45.000004	42
CGGTTGA	20	7.0288515E-4	45.000004	41
>>END_MODULE