FastQCFastQC Report
Thu 26 May 2016
SRR1043433_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043433_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1311791
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT624034.7570840171948126No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69620.5307247877138965No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27770.21169530817028015No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGAG302.165738E-645.0000042
GTCATCG302.165738E-645.00000422
CGAACGG302.165738E-645.0000041
CGTGATA302.165738E-645.0000049
TAGCGGA750.045.0000042
CTGCGAC302.165738E-645.00000411
GTCGATA207.033652E-445.028
CGGCCCA207.033652E-445.05
CGACATC351.2121018E-745.010
CGTTAGC207.033652E-445.045
TAACCGC207.033652E-445.021
TTAAGCG253.8911086E-545.012
CGAATAA253.8911086E-545.013
GCGATCG207.033652E-445.08
ACGCTAA351.2121018E-745.037
ACGTCAA207.033652E-445.032
ACCGTGC207.033652E-445.013
TTATTCG207.033652E-445.035
TATCGAC253.8911086E-545.026
TGTCGAT207.033652E-445.027