##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043433_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1311791
Sequences flagged as poor quality	0
Sequence length	51
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.537357704085483	31.0	31.0	33.0	30.0	34.0
2	30.54212218257329	31.0	31.0	34.0	26.0	34.0
3	31.485092518549067	31.0	31.0	34.0	28.0	34.0
4	35.0954161143048	37.0	35.0	37.0	32.0	37.0
5	35.4257194934254	37.0	35.0	37.0	33.0	37.0
6	35.86611129364358	37.0	35.0	37.0	35.0	37.0
7	33.72003924405641	37.0	35.0	37.0	32.0	37.0
8	34.768568316141824	37.0	35.0	37.0	31.0	37.0
9	36.76477579126553	39.0	37.0	39.0	32.0	39.0
10	36.71227962381202	39.0	35.0	39.0	32.0	39.0
11	36.427140451489606	39.0	35.0	39.0	32.0	39.0
12	36.366606418248026	39.0	35.0	39.0	32.0	39.0
13	36.3845368660099	39.0	35.0	39.0	32.0	39.0
14	37.73278822617323	40.0	36.0	41.0	33.0	41.0
15	37.90012814541341	40.0	36.0	41.0	33.0	41.0
16	37.89027977779997	40.0	36.0	41.0	33.0	41.0
17	37.82729947072362	40.0	36.0	41.0	33.0	41.0
18	37.53149930133687	39.0	36.0	41.0	33.0	41.0
19	37.21215346042167	38.0	35.0	41.0	33.0	41.0
20	36.88298669528911	38.0	35.0	40.0	32.0	41.0
21	36.7919013013506	38.0	35.0	40.0	32.0	41.0
22	36.70517407117445	38.0	35.0	40.0	32.0	41.0
23	36.59709054262455	37.0	35.0	40.0	32.0	41.0
24	36.43989858140512	37.0	35.0	40.0	32.0	41.0
25	36.37288943131947	37.0	35.0	40.0	32.0	41.0
26	36.11090791139747	37.0	35.0	40.0	31.0	41.0
27	36.01811950226827	37.0	35.0	40.0	31.0	41.0
28	35.94064527047372	36.0	35.0	40.0	31.0	41.0
29	35.862657237319056	36.0	35.0	40.0	31.0	41.0
30	35.64766414771865	36.0	35.0	40.0	30.0	41.0
31	35.15751823270628	36.0	34.0	40.0	27.0	41.0
32	34.92316611411422	36.0	34.0	40.0	25.0	41.0
33	34.71754570659503	36.0	34.0	40.0	24.0	41.0
34	34.45344875822444	36.0	34.0	40.0	22.0	41.0
35	34.190635550937614	36.0	34.0	40.0	21.0	41.0
36	34.00591100259111	36.0	34.0	40.0	20.0	41.0
37	33.910767035297546	36.0	33.0	40.0	18.0	41.0
38	33.81494536858387	36.0	33.0	40.0	18.0	41.0
39	33.693400854251934	36.0	33.0	40.0	18.0	41.0
40	33.65686302162463	36.0	33.0	40.0	18.0	41.0
41	33.60224456487352	36.0	33.0	40.0	18.0	41.0
42	33.51969406711893	35.0	33.0	40.0	18.0	41.0
43	33.36232524845803	35.0	33.0	40.0	17.0	41.0
44	33.17902242049229	35.0	33.0	40.0	15.0	41.0
45	32.98960962531379	35.0	33.0	40.0	15.0	41.0
46	33.00596436475018	35.0	33.0	40.0	15.0	41.0
47	32.99022862635893	35.0	33.0	40.0	15.0	41.0
48	32.927293295959494	35.0	33.0	40.0	15.0	41.0
49	32.845540943641176	35.0	33.0	40.0	15.0	41.0
50	32.723284425644025	35.0	33.0	40.0	14.0	41.0
51	32.29700005564911	35.0	31.0	39.0	12.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	3.0
12	9.0
13	12.0
14	25.0
15	64.0
16	147.0
17	369.0
18	744.0
19	1413.0
20	2407.0
21	3960.0
22	6426.0
23	10310.0
24	16747.0
25	25427.0
26	32280.0
27	34794.0
28	33716.0
29	33933.0
30	35933.0
31	41207.0
32	50392.0
33	66368.0
34	110880.0
35	146795.0
36	134684.0
37	120508.0
38	157332.0
39	244798.0
40	106.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.568637839411917	18.458047051702593	25.39306947524415	29.580245633641333
2	33.0490908993887	19.706035488885043	29.290412878271006	17.954460733455253
3	20.721136217583442	21.092231918041822	39.723096133454185	18.46353573092055
4	21.432682492866622	24.73999287996335	36.97829913454201	16.849025492628016
5	19.153584679266743	31.18019562567513	34.2777927276525	15.38842696740563
6	77.07401560157068	2.2121664198031543	19.448829882199224	1.264988096426946
7	73.17004004448879	7.65868953209772	17.55066165265656	1.6206087707569272
8	71.48318596483738	4.367845182654858	21.03681150427164	3.1121573482361136
9	40.52444329927557	20.04610490543082	27.78399912790986	11.645452667383752
10	30.520105718060268	24.480576555259184	35.17099903871882	9.828318687961726
11	27.947820956234644	21.465919494797568	38.739860236882244	11.846399312085538
12	21.606185741478633	26.800229609747284	36.85000125782232	14.743583390951759
13	15.957801204612625	29.31061426705931	37.41076131792336	17.320823210404708
14	15.431040463000583	24.293199145290675	43.67616487687444	16.5995955148343
15	17.62125216593192	22.324821560751676	43.735015715155846	16.31891055816056
16	19.986567982247173	22.4320032688134	36.53889987048242	21.04252887845701
17	20.04488519893794	22.996422448393076	40.70389261704037	16.254799735628616
18	23.696533975305517	24.945589655669234	36.304792455505485	15.05308391351976
19	23.600100930712287	24.878048408626068	36.141809175394556	15.38004148526709
20	19.550217984419774	27.890266056101925	37.125426230245516	15.434089729232781
21	18.56286557843437	25.304564522854633	37.12001378268337	19.01255611602763
22	16.768067474163185	27.53212973713038	36.756922406084506	18.942880382621926
23	16.074511869649967	25.248534255838013	43.78105963526202	14.895894239250001
24	16.696714644329774	25.294654407599992	41.49921748205316	16.50941346601707
25	16.38126805260899	28.870376454785863	38.11819108379307	16.630164408812075
26	15.897273269903515	29.60723163979628	37.882482804044244	16.613012286255966
27	16.06666000910206	27.586787834342513	41.75070571455362	14.595846442001815
28	15.632520729293004	26.095163025207523	40.31053727308695	17.961778972412525
29	16.525345882080302	28.30687205507585	39.538767989717876	15.629014073125976
30	19.767325740152202	27.9703092946971	36.6454717252977	15.616893239852994
31	20.235769265073476	26.959020148788948	37.51031986040459	15.294890725732987
32	18.563704126648222	28.5320603663236	36.20569130295909	16.698544204069094
33	16.13938500873996	28.363817101962123	36.45611229227826	19.040685597019646
34	16.64929855441911	28.198623103832855	37.2705713028981	17.88150703884994
35	20.990920047477076	28.14739543113194	34.65628289872396	16.205401622667026
36	17.24611618771588	30.92238016574287	34.66718402550406	17.164319621037194
37	17.3129713498568	31.72555689130357	33.87246901373771	17.089002745101926
38	17.50088238141594	32.592082122838164	32.626233904638774	17.28080159110712
39	19.03931342721516	29.750165994430517	33.1497166850512	18.060803893303127
40	16.022750575358423	29.07460106068726	34.77749123145379	20.12515713250053
41	17.073222792350307	29.47557957022117	33.891526927688936	19.559670709739585
42	17.286061575357657	28.38737268360585	34.81301518305889	19.513550557977606
43	16.90581807620269	29.7097632168539	34.93971219500667	18.444706511936733
44	15.252963315040278	33.00815450022145	33.214666055797	18.524216128941273
45	17.127728426250826	30.866807288661075	34.32147346642872	17.683990818659375
46	16.888589721990773	30.629574375796143	33.329852087718244	19.151983814494837
47	16.2390960145328	29.107838062618207	36.02136315922277	18.63170276362622
48	16.755946640890205	28.172094487612735	35.77803171389345	19.293927157603612
49	17.08412391913041	30.571257159105375	34.28503473495397	18.059584186810245
50	15.650663863374579	30.30841040988999	34.41805897433356	19.62286675240187
51	15.727657835737553	28.58023877279231	35.92858923410817	19.763514157361957
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1002.0
1	7256.5
2	13511.0
3	49693.0
4	85875.0
5	53433.5
6	20992.0
7	20645.5
8	20299.0
9	22325.5
10	24352.0
11	25473.0
12	26594.0
13	25860.0
14	25126.0
15	22613.5
16	20101.0
17	17476.0
18	14851.0
19	13078.5
20	11306.0
21	10864.5
22	10423.0
23	10104.5
24	9786.0
25	9550.5
26	10116.0
27	10917.0
28	12736.5
29	14556.0
30	17116.5
31	19677.0
32	21474.0
33	23271.0
34	28141.5
35	33012.0
36	37273.0
37	41534.0
38	46862.0
39	52190.0
40	59192.5
41	66195.0
42	76819.5
43	87444.0
44	96882.5
45	106321.0
46	109976.0
47	113631.0
48	106487.5
49	99344.0
50	91282.5
51	83221.0
52	75535.5
53	67850.0
54	61303.5
55	54757.0
56	49261.0
57	43765.0
58	38940.5
59	34116.0
60	28809.0
61	23502.0
62	19030.0
63	14558.0
64	12198.0
65	9838.0
66	8136.5
67	6435.0
68	5061.5
69	3688.0
70	3235.5
71	2783.0
72	2638.5
73	2494.0
74	1948.0
75	1121.5
76	841.0
77	624.0
78	407.0
79	282.0
80	157.0
81	139.0
82	121.0
83	93.0
84	65.0
85	69.5
86	74.0
87	50.5
88	27.0
89	36.5
90	46.0
91	30.5
92	15.0
93	9.0
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1311791.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.54494458982886
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.20842413971366	27.79914102503956
2	9.805570810013853	6.970769422271713
3	3.50155716097593	3.733879657950237
4	1.8195508881508977	2.5870334199079017
5	1.0812697134162865	1.9216836025021018
6	0.7262787062595532	1.5489321822461044
7	0.5625114048595695	1.399610570181613
8	0.42093377809365085	1.196965425466091
9	0.3227978595485814	1.0326448828232715
>10	2.8162366121192592	21.70803940483072
>50	0.5403309303058363	13.507114541804988
>100	0.19027476481522324	9.890936184546689
>500	0.0031413286414953413	0.7605528924598285
>1k	6.731418517490018E-4	0.4104242014050207
>5k	2.2438061724966725E-4	0.5552610357912464
>10k+	2.2438061724966725E-4	4.977011550772931
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	62403	4.7570840171948126	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6962	0.5307247877138965	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2777	0.21169530817028015	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.09384116829586421	0.0
2	0.0	0.0	0.0	0.3072898045496577	0.0
3	0.0	0.0	0.0	0.5070167427585645	0.0
4	0.0	0.0	0.0	0.6337899863621568	0.0
5	0.0	0.0	0.0	1.3897030853238055	0.0
6	0.0	0.0	0.0	1.5922505947974943	0.0
7	0.0	0.0	0.0	2.18068274595572	0.0
8	0.0	0.0	0.0	2.88353861247714	0.0
9	0.0	0.0	0.0	3.429281036384607	0.0
10	0.0	0.0	0.0	3.836434310038718	0.0
11	0.0	0.0	0.0	4.267524323615576	0.0
12	0.0	0.0	0.0	4.620171963369165	0.0
13	0.0	0.0	0.0	4.7851372665310254	0.0
14	0.0	0.0	0.0	4.9163319461713035	0.0
15	0.0	0.0	0.0	5.38004910843267	0.0
16	0.0	0.0	0.0	5.900253927645486	0.0
17	0.0	0.0	0.0	6.499815900551231	0.0
18	0.0	0.0	0.0	6.853378320174479	0.0
19	0.0	0.0	0.0	7.246276274193069	0.0
20	0.0	0.0	0.0	7.69505203191667	0.0
21	0.0	0.0	0.0	8.1814099959521	0.0
22	1.5246331160985248E-4	0.0	0.0	8.658467697979328	0.0
23	1.5246331160985248E-4	0.0	0.0	9.062800400368657	0.0
24	0.0010672431812689673	0.0	0.0	9.42047932940537	0.0
25	0.0010672431812689673	0.0	0.0	9.762530769002074	0.0
26	0.0010672431812689673	0.0	0.0	10.140411086827093	0.0
27	0.0010672431812689673	0.0	0.0	10.501596672030834	0.0
28	0.0010672431812689673	0.0	0.0	10.8941134677704	0.0
29	0.0010672431812689673	0.0	0.0	11.333665195141604	0.0
30	0.0010672431812689673	0.0	0.0	11.773521849136028	0.0
31	0.0010672431812689673	0.0	0.0	12.160016344067005	0.0
32	0.0010672431812689673	0.0	0.0	12.575707563171267	0.0
33	0.0010672431812689673	0.0	0.0	13.003595847204318	0.0
34	0.0010672431812689673	0.0	0.0	13.464339974889292	0.0
35	0.0010672431812689673	0.0	0.0	13.8760671478917	0.0
36	0.0010672431812689673	0.0	0.0	14.287641857582496	0.0
37	0.0010672431812689673	0.0	0.0	14.733749507352924	0.0
38	0.0010672431812689673	0.0	0.0	15.189614809066383	0.0
39	0.0010672431812689673	0.0	0.0	15.66476671969849	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCGAG	30	2.165738E-6	45.000004	2
GTCATCG	30	2.165738E-6	45.000004	22
CGAACGG	30	2.165738E-6	45.000004	1
CGTGATA	30	2.165738E-6	45.000004	9
TAGCGGA	75	0.0	45.000004	2
CTGCGAC	30	2.165738E-6	45.000004	11
GTCGATA	20	7.033652E-4	45.0	28
CGGCCCA	20	7.033652E-4	45.0	5
CGACATC	35	1.2121018E-7	45.0	10
CGTTAGC	20	7.033652E-4	45.0	45
TAACCGC	20	7.033652E-4	45.0	21
TTAAGCG	25	3.8911086E-5	45.0	12
CGAATAA	25	3.8911086E-5	45.0	13
GCGATCG	20	7.033652E-4	45.0	8
ACGCTAA	35	1.2121018E-7	45.0	37
ACGTCAA	20	7.033652E-4	45.0	32
ACCGTGC	20	7.033652E-4	45.0	13
TTATTCG	20	7.033652E-4	45.0	35
TATCGAC	25	3.8911086E-5	45.0	26
TGTCGAT	20	7.033652E-4	45.0	27
>>END_MODULE