##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043432_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3099779
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.52945774521345	31.0	31.0	33.0	30.0	34.0
2	30.578121859655155	31.0	31.0	34.0	26.0	34.0
3	31.560380917478312	31.0	31.0	34.0	28.0	34.0
4	35.17101283672159	37.0	35.0	37.0	32.0	37.0
5	35.496522171419315	37.0	35.0	37.0	33.0	37.0
6	35.75411343840964	37.0	35.0	37.0	35.0	37.0
7	33.756636521506856	37.0	35.0	37.0	32.0	37.0
8	34.74129123398797	37.0	35.0	37.0	31.0	37.0
9	36.74422402371266	39.0	37.0	39.0	32.0	39.0
10	36.72658018523256	39.0	35.0	39.0	32.0	39.0
11	36.556829373965044	39.0	35.0	39.0	32.0	39.0
12	36.55878757808218	39.0	35.0	39.0	33.0	39.0
13	36.586038230467395	39.0	35.0	39.0	33.0	39.0
14	37.85018544870457	40.0	36.0	41.0	33.0	41.0
15	37.932701008684816	40.0	36.0	41.0	33.0	41.0
16	37.786593173255255	40.0	36.0	41.0	33.0	41.0
17	37.762580171038	40.0	36.0	41.0	33.0	41.0
18	37.60286072007069	39.0	36.0	41.0	33.0	41.0
19	37.436917277005875	39.0	35.0	41.0	33.0	41.0
20	37.242171135426105	39.0	35.0	41.0	32.0	41.0
21	37.14165074348849	39.0	35.0	41.0	32.0	41.0
22	37.07032985254755	39.0	35.0	41.0	32.0	41.0
23	37.014504582423456	38.0	35.0	41.0	32.0	41.0
24	36.888290423285014	38.0	35.0	41.0	32.0	41.0
25	36.7907170156324	38.0	35.0	41.0	32.0	41.0
26	36.52551617389498	38.0	35.0	40.0	31.0	41.0
27	36.41920956300433	38.0	35.0	40.0	31.0	41.0
28	36.24413192037239	38.0	35.0	40.0	31.0	41.0
29	36.19175431538829	38.0	35.0	40.0	30.0	41.0
30	36.077541011794715	38.0	35.0	40.0	30.0	41.0
31	35.90157298310621	38.0	35.0	40.0	30.0	41.0
32	35.852017837400666	38.0	35.0	40.0	30.0	41.0
33	35.65855727134096	38.0	35.0	40.0	28.0	41.0
34	35.57085585778857	38.0	35.0	40.0	28.0	41.0
35	35.425809065743074	38.0	35.0	40.0	27.0	41.0
36	35.31392979951151	38.0	35.0	40.0	27.0	41.0
37	35.20457845543182	38.0	34.0	40.0	26.0	41.0
38	35.09040257386091	37.0	34.0	40.0	26.0	41.0
39	34.95438094135098	37.0	34.0	40.0	25.0	41.0
40	34.86333574103186	37.0	34.0	40.0	25.0	41.0
41	34.76219659530567	37.0	34.0	40.0	25.0	41.0
42	34.64068438427385	36.0	34.0	40.0	24.0	41.0
43	34.46847372022328	36.0	34.0	40.0	23.0	41.0
44	34.25481139139274	36.0	33.0	40.0	23.0	41.0
45	34.042192685349505	35.0	33.0	40.0	23.0	41.0
46	34.020687926461854	35.0	33.0	40.0	23.0	41.0
47	33.985397346068865	35.0	33.0	40.0	23.0	41.0
48	33.88081118040996	35.0	33.0	40.0	23.0	41.0
49	33.839506622891506	35.0	33.0	40.0	23.0	41.0
50	33.75517286877548	35.0	33.0	39.0	23.0	41.0
51	33.323627910247794	35.0	33.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	2.0
10	3.0
11	11.0
12	15.0
13	21.0
14	64.0
15	149.0
16	296.0
17	690.0
18	1467.0
19	2669.0
20	4508.0
21	7406.0
22	11638.0
23	16975.0
24	25800.0
25	38380.0
26	51007.0
27	60263.0
28	65906.0
29	70951.0
30	80433.0
31	94292.0
32	115556.0
33	151993.0
34	265521.0
35	308606.0
36	304421.0
37	349707.0
38	451604.0
39	619009.0
40	414.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.419917355398564	17.84365917699294	23.98767783122603	26.748745636382466
2	30.325613535674638	21.30119598848821	28.208881988038502	20.164308487798646
3	27.38788797523953	21.64160735329841	31.526570119998876	19.443934551463187
4	23.656170326981375	25.076561909736146	28.20381711083274	23.06345065244974
5	21.69522407887788	34.6865695909289	26.50543796831968	17.11276836187354
6	84.24055392336034	2.2544510431227516	12.052214044936752	1.4527809885801535
7	79.84156289851632	7.315263442974483	11.2240582312481	1.6191154272611048
8	78.47233625364906	4.23788276519068	14.115232085900317	3.174548895259952
9	41.7826561183878	22.61906413328176	20.623244431296552	14.97503531703389
10	29.01010039747995	19.773829037489445	29.72031231903952	21.495758245991084
11	26.168542983225578	16.99121130893525	37.055770750108316	19.784474957730858
12	22.206712155931115	20.13788724938133	37.40340843653693	20.25199215815063
13	18.263495558876937	24.33418640490177	37.612745940920306	19.789572095300986
14	18.062965133966003	21.288001499461735	39.115401452813245	21.533631913759013
15	22.00473001462362	16.677737348372254	39.34586949585761	21.971663141146514
16	21.903174387593438	18.249236477826322	34.128142683720355	25.719446450859884
17	22.0662827898376	22.20138919581041	33.807377880810215	21.924950133541778
18	23.880605681888934	24.864030629280347	30.947335277773032	20.30802841105769
19	25.144050591993818	22.016117923245496	30.487721866623396	22.35210961813729
20	25.240960726555024	23.670364887303254	32.555127317141	18.533547069000726
21	22.27229747669108	19.941615192566953	32.87556951640746	24.910517814334508
22	22.433179913793854	23.04754629281636	32.075060834982104	22.44421295840768
23	23.43734827547383	22.247489256492155	34.272862678274805	20.042299789759205
24	26.109022610966782	19.984973122277427	31.625770740430205	22.280233526325585
25	20.886553525267445	22.825788548151333	31.315619597397102	24.972038329184112
26	19.50903596675763	22.041635871460514	34.26137798855983	24.187950173222024
27	21.10856935284741	22.588416787132243	34.90058484814563	21.40242901187472
28	21.521824620400356	24.37744755351914	32.33285340664609	21.76787441943442
29	21.535567535621087	22.41501732865472	36.01979366916158	20.029621466562617
30	27.562222984283718	22.028925287899558	30.96985301210183	19.438998715714895
31	25.845229611530367	23.736305072071268	29.834836612545608	20.583628703852757
32	27.348369028888836	21.188220192471785	28.751565837435507	22.711844941203875
33	22.22103575771047	23.669784200744633	31.18486834061396	22.92431170093094
34	22.20729284248974	23.25869037760434	31.191384934216277	23.342631845689642
35	26.505212145769104	25.94981771281114	27.758204697818776	19.786765443600977
36	23.38686080523805	25.707445595315022	28.180589648487842	22.725103950959085
37	23.57332571128458	28.65313946574901	27.432471798795977	20.341063024170435
38	22.908246039475717	25.262478389588416	26.83936499989193	24.989910571043936
39	22.724652305857933	22.585674656160972	32.35427428858638	22.335398749394713
40	21.456819986199015	23.002026918693236	31.69693710422582	23.84421599088193
41	21.268903363755932	27.132224587623828	27.066897349778806	24.531974698841434
42	20.549239155436567	22.428695723146717	29.33125232476251	27.690812796654214
43	21.3285527774722	22.025376647819087	33.21811006526595	23.42796050944277
44	24.145624575171325	24.595301794095644	29.81254470076738	21.446528929965652
45	25.24234792222284	24.154915560109284	29.846805207726103	20.755931309941772
46	22.738266179621192	25.274543765862017	28.76005031326427	23.227139741252522
47	20.652633623235722	27.763656699396954	30.322000374865432	21.261709302501888
48	23.23888251388244	25.530691058943233	30.477946976219915	20.752479450954407
49	21.85788083602089	22.9319896676505	32.01657279438309	23.193556701945525
50	20.249120985721884	24.76495905030649	33.68520788094893	21.300712083022695
51	20.18682622212745	21.87672088881175	32.33549875652425	25.600954132536547
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1462.0
1	11590.0
2	21718.0
3	74681.5
4	127645.0
5	77510.5
6	27376.0
7	25143.0
8	22910.0
9	23223.5
10	23537.0
11	23496.0
12	23455.0
13	22641.0
14	21827.0
15	20418.5
16	19010.0
17	17439.5
18	15869.0
19	14617.0
20	13365.0
21	13667.5
22	13970.0
23	14047.5
24	14125.0
25	15416.0
26	19789.0
27	22871.0
28	23789.5
29	24708.0
30	30256.5
31	35805.0
32	41136.0
33	46467.0
34	53453.5
35	60440.0
36	67449.5
37	74459.0
38	79802.5
39	85146.0
40	95148.5
41	105151.0
42	113911.0
43	122671.0
44	134626.0
45	146581.0
46	156165.5
47	165750.0
48	190864.0
49	215978.0
50	209257.0
51	202536.0
52	179703.5
53	156871.0
54	157309.5
55	157748.0
56	164279.5
57	170811.0
58	174793.5
59	178776.0
60	173068.0
61	167360.0
62	161151.0
63	154942.0
64	144446.5
65	133951.0
66	113348.5
67	92746.0
68	79055.5
69	65365.0
70	57232.5
71	49100.0
72	46398.5
73	43697.0
74	36778.5
75	21576.5
76	13293.0
77	10116.5
78	6940.0
79	5444.5
80	3949.0
81	2749.5
82	1550.0
83	1210.0
84	870.0
85	556.5
86	243.0
87	171.0
88	99.0
89	75.0
90	51.0
91	31.0
92	11.0
93	7.5
94	4.0
95	2.5
96	1.0
97	1.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3099779.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.052720267063865
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.47258011025147	17.85953717649323
2	9.55538668944482	4.405553127907937
3	3.141090879959648	2.1723206816740572
4	1.5198682300210824	1.4014838859789396
5	0.9061494005321172	1.0444604325317255
6	0.6581674222937642	0.9103529685019583
7	0.44226415188071516	0.7136774244219461
8	0.3489892702119959	0.6436121617923123
9	0.28107765135030693	0.5831644022911739
>10	3.668202713844998	21.096185864105504
>50	1.4838845900153717	24.285418797797576
>100	0.5118837360570527	16.725542596600228
>500	0.005675655102760546	0.8991069380334534
>1k	0.003584624275427713	1.4073142832398169
>5k	5.974373792379522E-4	0.9552301069146439
>10k+	5.974373792379522E-4	4.897039151715465
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	87984	2.838395898546316	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	30316	0.9780052061776018	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	13899	0.4483868043496004	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	10027	0.3234746735170475	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9614	0.3101511430331001	No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	6873	0.22172548430065497	No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	6009	0.19385252948677956	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	5247	0.16927013183843106	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3638	0.11736320557046165	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.038938259792069045	0.0
2	0.0	0.0	0.0	0.1300092684026829	0.0
3	0.0	0.0	0.0	0.2064663319546329	0.0
4	0.0	0.0	0.0	0.25611503271684855	0.0
5	0.0	0.0	0.0	0.5499101710154175	0.0
6	0.0	0.0	0.0	0.6314966325018655	0.0
7	0.0	0.0	0.0	0.8864502921014692	0.0
8	0.0	0.0	0.0	1.1676316279321848	0.0
9	0.0	0.0	0.0	1.4159396524719987	0.0
10	0.0	0.0	0.0	1.5881777378322777	0.0
11	0.0	0.0	0.0	1.779255875983417	3.226036436791139E-5
12	0.0	0.0	0.0	1.9370413181068715	3.226036436791139E-5
13	0.0	0.0	0.0	2.0126273518208877	3.226036436791139E-5
14	0.0	0.0	0.0	2.0717928600716373	3.226036436791139E-5
15	0.0	0.0	0.0	2.2620322287492107	3.226036436791139E-5
16	0.0	0.0	0.0	2.479692907139509	3.226036436791139E-5
17	3.226036436791139E-5	0.0	0.0	2.761196846613904	3.226036436791139E-5
18	3.226036436791139E-5	0.0	0.0	2.915788512664935	6.452072873582278E-5
19	3.226036436791139E-5	0.0	0.0	3.0900590009803923	6.452072873582278E-5
20	3.226036436791139E-5	0.0	0.0	3.291912100830414	6.452072873582278E-5
21	3.226036436791139E-5	0.0	0.0	3.494087804324115	6.452072873582278E-5
22	3.226036436791139E-5	0.0	0.0	3.709651559030499	6.452072873582278E-5
23	6.452072873582278E-5	0.0	0.0	3.8932452926482823	6.452072873582278E-5
24	1.6130182183955694E-4	0.0	0.0	4.0500629238406995	6.452072873582278E-5
25	1.6130182183955694E-4	0.0	0.0	4.208267750700937	6.452072873582278E-5
26	1.6130182183955694E-4	0.0	0.0	4.3731827333497	6.452072873582278E-5
27	1.6130182183955694E-4	0.0	0.0	4.557324893161739	6.452072873582278E-5
28	1.6130182183955694E-4	0.0	0.0	4.744918911961143	6.452072873582278E-5
29	1.6130182183955694E-4	0.0	0.0	4.945739680151392	6.452072873582278E-5
30	1.6130182183955694E-4	0.0	0.0	5.145108731945084	6.452072873582278E-5
31	1.6130182183955694E-4	0.0	0.0	5.34257442224107	6.452072873582278E-5
32	1.6130182183955694E-4	0.0	0.0	5.56652587168311	6.452072873582278E-5
33	1.6130182183955694E-4	0.0	0.0	5.781928324567654	6.452072873582278E-5
34	1.6130182183955694E-4	0.0	0.0	6.044172826514407	6.452072873582278E-5
35	1.6130182183955694E-4	0.0	0.0	6.263994949317355	6.452072873582278E-5
36	1.6130182183955694E-4	0.0	0.0	6.497076081875514	6.452072873582278E-5
37	1.6130182183955694E-4	0.0	0.0	6.765869437788952	6.452072873582278E-5
38	1.6130182183955694E-4	0.0	0.0	7.04995420641278	6.452072873582278E-5
39	1.6130182183955694E-4	0.0	0.0	7.397720934298865	6.452072873582278E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATGCGA	25	3.8923434E-5	45.0	1
CTTACCG	20	7.0351415E-4	45.0	38
CGTTTTT	23205	0.0	44.04977	1
TAGTAGG	735	0.0	42.551018	1
ACTACGG	175	0.0	42.42857	1
GACGGTC	1185	0.0	41.96203	28
TATACGG	280	0.0	41.785717	1
CGACGGT	1190	0.0	41.785713	27
CGGTCTA	1205	0.0	41.452282	30
TACGGGT	235	0.0	41.17021	3
TAGGGTA	1690	0.0	41.00592	4
CTAACCT	1250	0.0	40.859997	14
TCACGAC	1235	0.0	40.627533	24
GCGCGAC	4005	0.0	40.449436	8
CACTTAC	10465	0.0	40.42045	36
CATATGC	2495	0.0	40.31062	32
TAGCATA	2540	0.0	40.03937	29
CGCACTT	10510	0.0	40.0333	34
ACTTACT	10530	0.0	40.02137	37
GCACTTA	10540	0.0	39.87666	35
>>END_MODULE