FastQCFastQC Report
Thu 26 May 2016
SRR1043429_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043429_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2801296
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT873453.1180210873824117No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC239980.856674910470011No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94850.3385932796819758No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC93360.33327431303225363No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC72780.2598083172931386No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA50230.1793098622923104No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC49930.17823892940981603No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC46270.16517354824338448No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35960.12836915484832737No Hit
TTACTGGGAATTCCTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCAT34280.12237193070635877No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT243150.043.6489831
TATACGG1550.043.5483861
TTAATCG2300.042.0652219
CATATGC27300.041.37362732
TCACGAC6050.041.28099424
ACTTACT73550.041.11488737
CACTTAC74300.041.00269336
CGCACTT74150.040.8732334
CGGTCTA6050.040.53719330
CTTACTG74700.040.42168438
ACGTAGG1900.040.2631571
TAGCATA27950.040.25044629
ACCCGCA75100.040.2363531
GCACTTA75600.040.1785735
ACGACCA5950.040.08403427
CCGCACT75850.039.98681333
CATGCGG4900.039.948981
GCCAGTA29150.039.7512924
CCAGTAG29150.039.67410325
GTTGATC29550.039.6700515