##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043429_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2801296
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.51875453361587	31.0	31.0	33.0	30.0	34.0
2	30.58161686590778	31.0	31.0	34.0	26.0	34.0
3	31.55334530874281	31.0	31.0	34.0	28.0	34.0
4	35.175279584877856	37.0	35.0	37.0	32.0	37.0
5	35.49034625401957	37.0	35.0	37.0	33.0	37.0
6	35.73915751852	37.0	35.0	37.0	35.0	37.0
7	33.73898759716931	37.0	35.0	37.0	32.0	37.0
8	34.72647803016889	37.0	35.0	37.0	31.0	37.0
9	36.72292467486478	39.0	37.0	39.0	32.0	39.0
10	36.68615954900874	39.0	35.0	39.0	32.0	39.0
11	36.494705307828944	39.0	35.0	39.0	32.0	39.0
12	36.47381390613487	39.0	35.0	39.0	32.0	39.0
13	36.477626070218925	39.0	35.0	39.0	32.0	39.0
14	37.742748356475005	40.0	36.0	41.0	33.0	41.0
15	37.8318988782335	40.0	36.0	41.0	33.0	41.0
16	37.6449843215426	40.0	36.0	41.0	32.0	41.0
17	37.639986634757626	40.0	36.0	41.0	32.0	41.0
18	37.44621096806621	39.0	36.0	41.0	32.0	41.0
19	37.26228716993849	39.0	35.0	41.0	32.0	41.0
20	37.033055414351075	39.0	35.0	41.0	32.0	41.0
21	36.94017197754182	38.0	35.0	41.0	32.0	41.0
22	36.84740884219304	38.0	35.0	40.0	32.0	41.0
23	36.789093333942574	38.0	35.0	40.0	32.0	41.0
24	36.66795940164838	38.0	35.0	40.0	32.0	41.0
25	36.553111845374424	38.0	35.0	40.0	31.0	41.0
26	36.28418417760922	38.0	35.0	40.0	31.0	41.0
27	36.156819557804674	38.0	35.0	40.0	31.0	41.0
28	35.98889870974006	37.0	35.0	40.0	30.0	41.0
29	35.88958610585957	37.0	35.0	40.0	30.0	41.0
30	35.72972831146726	37.0	35.0	40.0	30.0	41.0
31	35.54046805478607	37.0	34.0	40.0	29.0	41.0
32	35.45520823218967	37.0	34.0	40.0	28.0	41.0
33	35.21724980152044	37.0	34.0	40.0	26.0	41.0
34	35.10228265774128	37.0	34.0	40.0	26.0	41.0
35	34.95981931220407	37.0	34.0	40.0	25.0	41.0
36	34.82665309199742	37.0	34.0	40.0	25.0	41.0
37	34.70023339197286	37.0	34.0	40.0	24.0	41.0
38	34.56669805689938	36.0	34.0	40.0	23.0	41.0
39	34.43020766102547	36.0	34.0	40.0	23.0	41.0
40	34.32544900645987	36.0	34.0	40.0	23.0	41.0
41	34.22712344571941	36.0	33.0	40.0	23.0	41.0
42	34.10489787584032	35.0	33.0	40.0	23.0	41.0
43	33.93714088050674	35.0	33.0	40.0	23.0	41.0
44	33.713732857934325	35.0	33.0	40.0	22.0	41.0
45	33.50935174290757	35.0	33.0	40.0	21.0	41.0
46	33.48259948252523	35.0	33.0	39.0	22.0	41.0
47	33.43987318726761	35.0	33.0	39.0	22.0	41.0
48	33.337354567314556	35.0	33.0	39.0	22.0	41.0
49	33.29317929986692	35.0	33.0	39.0	22.0	41.0
50	33.21072817724367	35.0	33.0	39.0	21.0	41.0
51	32.78371046829753	35.0	32.0	38.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	6.0
10	2.0
11	9.0
12	18.0
13	31.0
14	78.0
15	145.0
16	292.0
17	765.0
18	1471.0
19	2785.0
20	4929.0
21	7534.0
22	11754.0
23	17746.0
24	26733.0
25	38558.0
26	50749.0
27	59410.0
28	65248.0
29	69952.0
30	77936.0
31	90854.0
32	111357.0
33	147059.0
34	262091.0
35	284558.0
36	283721.0
37	316236.0
38	389279.0
39	479714.0
40	274.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.06126450043123	17.30763189609381	23.650231892666824	28.98087171080814
2	30.691544199541926	21.399059578138118	27.073683038136632	20.835713184183323
3	27.001537859619262	21.95019733723248	31.852435444165845	19.195829358982415
4	24.232569496404523	24.687823064752887	29.04380686653606	22.03580057230653
5	22.393492155059658	34.14615949189233	26.73708883316865	16.72325951987937
6	82.95453247354082	2.3260305230150617	13.230090643759173	1.4893463596849459
7	78.55139192716514	7.449266339579966	12.433030997081351	1.5663107361735424
8	77.92161199673295	3.7589387197925532	15.207318326945812	3.1121309565286923
9	45.47223856386473	18.435074337021153	21.90818107047595	14.184506028638172
10	29.757369446142075	19.399663584283847	28.489313517743213	22.353653451830866
11	26.30525299718416	15.552908368126753	37.9004575025274	20.241381132161685
12	21.924673436866364	18.41026439191003	38.1201772322525	21.544884938971105
13	18.394164700909865	22.054184920122687	39.97410484290128	19.57754553606616
14	18.738862298022056	19.022016952153574	40.79133372553275	21.447787024291614
15	23.264838846019842	15.376454326854427	39.79604440230522	21.562662424820513
16	23.579300438082946	16.303989296382817	35.04195915033613	25.074751115198108
17	24.11626618536563	19.67321554023566	34.23472564127461	21.975792633124097
18	24.714703480103495	22.77096029837618	31.48110731604229	21.033228905478037
19	25.936209525876595	20.83635574391282	30.86449986006477	22.362934870145818
20	25.707672448752295	22.50922430189455	33.397934384656246	18.38516886469691
21	23.38906706038919	18.487050279584878	34.05894985749453	24.0649328025314
22	23.65248085172006	20.943698916501504	33.030854290299914	22.372965941478515
23	24.563809036960034	20.047792164769447	34.78771968403196	20.600679114238552
24	26.57013039678777	17.91245909036389	32.7488419645764	22.768568548271944
25	21.42536882928473	20.540314197428618	32.870178660163006	25.16413831312364
26	20.311634329253316	20.15852662481937	34.692228168676216	24.8376108772511
27	21.434721643125183	22.01716634015113	34.56871390956186	21.979398107161828
28	22.978150113376095	22.40031042774487	32.299442829319005	22.322096629560033
29	22.65062314014656	20.752751583552755	35.98284508313295	20.613780193167734
30	27.888305984087363	21.264550408096824	30.746375963125637	20.10076764469017
31	27.762221486055026	20.937487505783036	30.394645906751734	20.905645101410204
32	28.48702886092723	19.68935092899858	28.821659688943974	23.00196052113022
33	23.199868917815184	22.361649750686823	32.10949503372725	22.328986297770744
34	23.3777508695975	23.014704622431903	31.426561134560576	22.180983373410022
35	27.068578258063408	24.579730239146453	28.09927976193876	20.25241174085138
36	24.83786076159035	24.264626087353854	28.41581182424135	22.481701326814445
37	24.943740325906294	27.833331429452652	26.609397935812567	20.613530308828484
38	24.659836018757034	23.52668193578972	27.32092574294184	24.49255630251141
39	24.058864182863932	21.789021938417076	31.461295057716143	22.69081882100285
40	22.07071298427585	22.043689777874242	31.570458816205072	24.31513842164484
41	21.582546078672156	25.49266482371017	27.97926388357389	24.945525214043784
42	21.566374992146493	20.926635385907094	29.79595872767462	27.711030894271794
43	22.735726606542116	20.385957071298428	32.35052632781398	24.527789994345476
44	24.489343503863925	23.073677326494593	30.12676989507714	22.310209274564343
45	25.58894168984641	22.917713801040662	30.02638778622466	21.466956722888263
46	23.02491418257835	23.418482016895037	29.53022458176501	24.0263792187616
47	21.868056785145164	25.710778154111523	31.079221903004893	21.34194315773842
48	24.08945716554052	24.56687904455652	30.09935401328528	21.24430977661768
49	22.207506811133133	21.924387854764365	31.710893814862835	24.157211519239667
50	21.060930369371892	24.067717228025884	33.029319286501675	21.84203311610055
51	21.17002273233532	20.83007293766885	32.3769069744861	25.622997355509735
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1266.0
1	11380.0
2	21494.0
3	76133.0
4	130772.0
5	79213.5
6	27655.0
7	24947.0
8	22239.0
9	22432.0
10	22625.0
11	22568.0
12	22511.0
13	21606.0
14	20701.0
15	19321.5
16	17942.0
17	16723.0
18	15504.0
19	14883.5
20	14263.0
21	13384.0
22	12505.0
23	12531.0
24	12557.0
25	13247.0
26	15895.5
27	17854.0
28	19836.0
29	21818.0
30	24738.5
31	27659.0
32	32314.0
33	36969.0
34	41932.5
35	46896.0
36	49609.5
37	52323.0
38	55488.0
39	58653.0
40	66106.5
41	73560.0
42	84792.5
43	96025.0
44	102477.5
45	108930.0
46	116659.0
47	124388.0
48	141596.5
49	158805.0
50	159830.5
51	160856.0
52	150498.0
53	140140.0
54	143653.0
55	147166.0
56	157327.0
57	167488.0
58	170338.5
59	173189.0
60	173129.5
61	173070.0
62	169621.5
63	166173.0
64	152575.0
65	138977.0
66	121286.0
67	103595.0
68	89080.5
69	74566.0
70	64704.5
71	54843.0
72	51613.0
73	48383.0
74	40964.5
75	26134.0
76	18722.0
77	14494.0
78	10266.0
79	7937.5
80	5609.0
81	4060.0
82	2511.0
83	1867.5
84	1224.0
85	964.0
86	704.0
87	487.5
88	271.0
89	173.0
90	75.0
91	58.0
92	41.0
93	27.0
94	13.0
95	14.0
96	15.0
97	8.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2801296.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.544220368959493
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.86276657694788	19.110809014014624
2	8.893912328252728	4.365882882536811
3	2.872664960971439	2.1152196554481426
4	1.4600600994587005	1.4334414733215703
5	0.8686347080538205	1.0659980847299884
6	0.6385672823044984	0.9403881658373507
7	0.472818135629031	0.8123466760723661
8	0.402916785092552	0.7911422690931433
9	0.31977507232395275	0.706376685925715
>10	4.51512586898611	27.477304235771477
>50	1.3981353338333982	23.56263405321062
>100	0.2859578600668875	10.107332157845178
>500	0.005570349479418714	0.9270367768424609
>1k	0.0021662470197739443	1.1735883030719265
>5k	6.189277199354127E-4	1.1819434071647303
>10k+	3.0946385996770637E-4	4.228556159113893
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	87345	3.1180210873824117	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	23998	0.856674910470011	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9485	0.3385932796819758	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	9336	0.33327431303225363	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	7278	0.2598083172931386	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	5023	0.1793098622923104	No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	4993	0.17823892940981603	No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	4627	0.16517354824338448	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3596	0.12836915484832737	No Hit
TTACTGGGAATTCCTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCAT	3428	0.12237193070635877	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03041449386284063	0.0
2	0.0	0.0	0.0	0.10384479183920585	0.0
3	0.0	0.0	0.0	0.1597474883054129	0.0
4	0.0	0.0	0.0	0.19765851234571427	0.0
5	0.0	0.0	0.0	0.429765365744998	0.0
6	0.0	0.0	0.0	0.508586025896585	0.0
7	0.0	0.0	0.0	0.7319112296594148	0.0
8	0.0	0.0	0.0	0.9705864714046641	0.0
9	0.0	0.0	0.0	1.1944114438459912	0.0
10	0.0	0.0	0.0	1.344484838446205	0.0
11	0.0	0.0	0.0	1.505910121600859	0.0
12	0.0	0.0	0.0	1.6404192916421543	0.0
13	0.0	0.0	0.0	1.7113507462260324	0.0
14	0.0	0.0	0.0	1.7626127335347639	0.0
15	0.0	0.0	0.0	1.9088664675207476	0.0
16	0.0	0.0	0.0	2.0791804936001053	0.0
17	0.0	0.0	0.0	2.3010063913274426	3.569776274981294E-5
18	0.0	0.0	0.0	2.4289471730227725	3.569776274981294E-5
19	0.0	0.0	0.0	2.575129511483256	3.569776274981294E-5
20	0.0	0.0	0.0	2.7409456194561375	3.569776274981294E-5
21	0.0	0.0	0.0	2.913115929198485	3.569776274981294E-5
22	3.569776274981294E-5	0.0	0.0	3.0860001941958295	3.569776274981294E-5
23	3.569776274981294E-5	0.0	0.0	3.244498260804999	3.569776274981294E-5
24	1.0709328824943883E-4	0.0	0.0	3.378007893489299	3.569776274981294E-5
25	1.0709328824943883E-4	0.0	0.0	3.5187998697745613	3.569776274981294E-5
26	1.0709328824943883E-4	0.0	0.0	3.668480588984527	3.569776274981294E-5
27	1.0709328824943883E-4	0.0	0.0	3.8281566817644403	3.569776274981294E-5
28	1.0709328824943883E-4	0.0	0.0	3.9945082561785688	3.569776274981294E-5
29	1.0709328824943883E-4	0.0	0.0	4.166357286056169	3.569776274981294E-5
30	1.0709328824943883E-4	0.0	0.0	4.346202614789726	3.569776274981294E-5
31	1.0709328824943883E-4	0.0	0.0	4.524370148674042	3.569776274981294E-5
32	1.0709328824943883E-4	0.0	0.0	4.722528429698254	3.569776274981294E-5
33	1.0709328824943883E-4	0.0	0.0	4.928468823001925	3.569776274981294E-5
34	1.0709328824943883E-4	0.0	0.0	5.160789862977707	3.569776274981294E-5
35	1.0709328824943883E-4	0.0	0.0	5.36062593885116	3.569776274981294E-5
36	1.0709328824943883E-4	0.0	0.0	5.576918683352277	3.569776274981294E-5
37	1.0709328824943883E-4	0.0	0.0	5.848114586962605	3.569776274981294E-5
38	1.0709328824943883E-4	0.0	0.0	6.146690674602041	3.569776274981294E-5
39	1.0709328824943883E-4	0.0	0.0	6.486533375980261	3.569776274981294E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	24315	0.0	43.648983	1
TATACGG	155	0.0	43.548386	1
TTAATCG	230	0.0	42.06522	19
CATATGC	2730	0.0	41.373627	32
TCACGAC	605	0.0	41.280994	24
ACTTACT	7355	0.0	41.114887	37
CACTTAC	7430	0.0	41.002693	36
CGCACTT	7415	0.0	40.87323	34
CGGTCTA	605	0.0	40.537193	30
CTTACTG	7470	0.0	40.421684	38
ACGTAGG	190	0.0	40.263157	1
TAGCATA	2795	0.0	40.250446	29
ACCCGCA	7510	0.0	40.23635	31
GCACTTA	7560	0.0	40.17857	35
ACGACCA	595	0.0	40.084034	27
CCGCACT	7585	0.0	39.986813	33
CATGCGG	490	0.0	39.94898	1
GCCAGTA	2915	0.0	39.75129	24
CCAGTAG	2915	0.0	39.674103	25
GTTGATC	2955	0.0	39.67005	15
>>END_MODULE