FastQCFastQC Report
Thu 26 May 2016
SRR1043428_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043428_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3905509
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1080992.767859451866581No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC412511.0562259618400571No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC148540.38033454794240645No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC117880.3018300559542943No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109300.2798610885290496No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC70780.18123117882969927No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA56810.14546119340654445No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC54400.1392904228360503No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG50130.1283571488377059No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46610.11934423912478502No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT337400.043.832991
TTAATCG5250.041.5714319
CGCACTT125000.041.25634
CTTACTG124700.041.13873338
GCACTTA125200.041.08226835
ACCCGCA126200.040.72107731
CCGCACT126500.040.67786433
TAATCGT5400.040.41666420
ACTTACT127550.040.39592437
CACTTAC127850.040.33633436
CGGTCTA8650.040.31791730
CCCGCAC128350.040.2317932
TCACGAC8850.039.91525324
ATAACGG1750.039.8571431
CACGACG8650.039.79768825
TCAACGC135400.039.71565615
GCTTATG133100.039.65815424
TAGTAGG8350.039.610781
AACGCAA135400.039.59933517
CAACGCA135700.039.59469616