##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043427_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3112489
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.530504043548426	31.0	31.0	33.0	30.0	34.0
2	30.57819256549983	31.0	31.0	34.0	26.0	34.0
3	31.545044175256525	31.0	31.0	34.0	28.0	34.0
4	35.15973678943122	37.0	35.0	37.0	32.0	37.0
5	35.48125182129158	37.0	35.0	37.0	33.0	37.0
6	35.77209718652821	37.0	35.0	37.0	35.0	37.0
7	33.78343345149172	37.0	35.0	37.0	32.0	37.0
8	34.753483787412584	37.0	35.0	37.0	31.0	37.0
9	36.72979149484544	39.0	37.0	39.0	32.0	39.0
10	36.72433380487449	39.0	35.0	39.0	32.0	39.0
11	36.55963249990602	39.0	35.0	39.0	32.0	39.0
12	36.55525947240295	39.0	35.0	39.0	33.0	39.0
13	36.58026903870182	39.0	35.0	39.0	33.0	39.0
14	37.82413463951198	40.0	36.0	41.0	33.0	41.0
15	37.905974286174185	40.0	36.0	41.0	33.0	41.0
16	37.74462785250004	40.0	36.0	41.0	33.0	41.0
17	37.683308760287986	40.0	36.0	41.0	32.0	41.0
18	37.51168245092593	39.0	36.0	41.0	32.0	41.0
19	37.356220375397314	39.0	35.0	41.0	32.0	41.0
20	37.14153335160381	39.0	35.0	41.0	32.0	41.0
21	37.01431137587956	39.0	35.0	41.0	32.0	41.0
22	36.961148135784576	38.0	35.0	41.0	32.0	41.0
23	36.90003081135387	38.0	35.0	40.0	32.0	41.0
24	36.77827809190651	38.0	35.0	40.0	32.0	41.0
25	36.661380329376264	38.0	35.0	40.0	31.0	41.0
26	36.40295628354028	38.0	35.0	40.0	31.0	41.0
27	36.27914765321259	38.0	35.0	40.0	31.0	41.0
28	36.10364984422435	38.0	35.0	40.0	30.0	41.0
29	36.01281675212346	38.0	35.0	40.0	30.0	41.0
30	35.87096821868286	38.0	35.0	40.0	30.0	41.0
31	35.696124548552625	38.0	35.0	40.0	29.0	41.0
32	35.60754142424278	38.0	35.0	40.0	29.0	41.0
33	35.390216961409344	38.0	34.0	40.0	27.0	41.0
34	35.220913551822996	38.0	34.0	40.0	26.0	41.0
35	35.100457865071974	38.0	34.0	40.0	25.0	41.0
36	35.01007007574967	37.0	34.0	40.0	25.0	41.0
37	34.89707722661831	37.0	34.0	40.0	25.0	41.0
38	34.77632692035217	37.0	34.0	40.0	24.0	41.0
39	34.65180407063286	37.0	34.0	40.0	24.0	41.0
40	34.55494396927989	37.0	34.0	40.0	23.0	41.0
41	34.45647775783304	36.0	34.0	40.0	23.0	41.0
42	34.323411263461495	36.0	33.0	40.0	23.0	41.0
43	34.133577660836714	36.0	33.0	40.0	23.0	41.0
44	33.90178407056218	35.0	33.0	40.0	22.0	41.0
45	33.69051264116917	35.0	33.0	40.0	22.0	41.0
46	33.65393966050964	35.0	33.0	40.0	22.0	41.0
47	33.635791162635435	35.0	33.0	40.0	22.0	41.0
48	33.523886510121	35.0	33.0	39.0	22.0	41.0
49	33.49500351647829	35.0	33.0	39.0	22.0	41.0
50	33.42387330525506	35.0	33.0	39.0	22.0	41.0
51	32.99378246798623	35.0	32.0	39.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	4.0
10	6.0
11	10.0
12	13.0
13	31.0
14	58.0
15	118.0
16	376.0
17	738.0
18	1537.0
19	2996.0
20	5120.0
21	7910.0
22	12540.0
23	18450.0
24	28068.0
25	41371.0
26	54888.0
27	65317.0
28	70607.0
29	75987.0
30	84540.0
31	99225.0
32	120134.0
33	158083.0
34	278652.0
35	305356.0
36	306069.0
37	352231.0
38	449900.0
39	571774.0
40	379.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.646598269102316	17.665443958195514	23.996390027402505	27.69156774529966
2	30.919755860984573	21.53119256003796	27.67942312406566	19.86962845491181
3	27.376064622236417	21.769651234108782	31.837381593959048	19.016902549695757
4	24.47202223044001	24.531620834643913	28.84443928958464	22.151917645331437
5	21.728430204893897	34.31687630060701	27.012946873065253	16.941746621433843
6	83.05815699268335	2.388634947786161	13.088945856515476	1.4642622030150148
7	78.68535439000748	7.497665051988939	12.253537281577541	1.563443276426037
8	77.6505876807918	4.0287692583009935	15.16760380518614	3.1530392557210645
9	43.531591597592794	19.538703590599034	21.879852426787693	15.049852385020476
10	29.231621380830582	19.074734079381486	29.09176546487393	22.601879074914
11	26.108750906428906	15.90158230278083	38.37706735670391	19.612599434086352
12	22.124801083634352	18.628435313345687	38.54281894650873	20.703944656511236
13	17.644110549467	22.278761467108797	40.00296868519053	20.074159298233667
14	17.959870701551072	19.759073847329258	41.70944861170594	20.571606839413732
15	22.716706789967773	15.583958690295773	40.67056943815705	21.028765081579404
16	23.006860425852107	16.795722008977382	34.79520730836318	25.402210256807333
17	23.025912702020797	20.40097169821323	34.74627540852353	21.826840191242443
18	24.66235222036126	23.492805918350236	31.082840774698322	20.762001086590185
19	25.99119225802886	21.159624981807166	30.652124393050062	22.197058367113907
20	26.99228816551641	22.072527806523976	32.487857788413066	18.44732623954655
21	23.034105502059607	19.35287160854223	32.83783492889453	24.775187960503636
22	24.201981115435267	21.504268770106496	32.20429052118738	22.089459593270853
23	25.017694841652453	20.24537275473102	34.410049320656235	20.326883082960293
24	26.76105843265631	18.18763054263003	32.11169581643502	22.939615208278646
25	21.84640652545278	21.017937734077133	31.70719639491095	25.428459345559133
26	20.37905997418786	20.864459280016735	34.297695509927905	24.4587852358675
27	21.207464508308302	22.100704612931963	34.766452186658334	21.9253786921014
28	22.698779015765197	23.146009512001488	32.14504533188712	22.0101661403462
29	22.962619305642527	21.084925922629765	35.70910612053569	20.24334865119202
30	27.616290370825407	21.24492648809361	31.449235643885004	19.689547497195974
31	27.901978127472898	21.386935022099678	30.38603509924051	20.325051751186912
32	27.176610102075866	20.76871596975925	29.30307544862006	22.75159847954483
33	23.69875042128663	22.64387119119136	32.0116151414511	21.64576324607091
34	23.893803319465547	22.658586102633617	31.399147113451647	22.048463464449192
35	26.525651978207794	25.402531543083366	28.698029133596936	19.373787345111904
36	24.66010321642904	24.763846554959713	28.608550905722076	21.96749932288917
37	25.15992827605174	27.10910785548158	27.024738079395622	20.706225789071063
38	23.887377593944912	24.54068110762801	26.9033561243108	24.668585174116277
39	24.031635131883196	21.476381121346936	31.939261472088738	22.55272227468113
40	20.871527578089434	22.20425517969702	32.42376117634472	24.50045606586883
41	21.704590763212337	26.84761295541928	27.35328542526576	24.094510856102623
42	21.857747930996705	21.700863842410367	29.467413378810335	26.973974847782596
43	22.380255801707253	21.053182838557824	31.955807715304374	24.61075364443055
44	23.861257019703526	23.305913691582525	30.045792932922815	22.787036355791138
45	25.830324219619733	22.636449478215024	30.304299870618017	21.22892643154723
46	23.150411133983123	24.303154163757686	29.963897061162303	22.582537641096884
47	22.032624051040823	25.146659152851626	30.89623770557904	21.92447909052851
48	22.14790156688104	25.258241876517474	31.43339623047664	21.16046032612485
49	22.328078910479686	22.461830387191732	32.954365461211275	22.255725241117318
50	20.73922188961953	23.079728153256124	33.47378898367191	22.707260973452435
51	20.72707726838553	21.09115887638478	33.93788700939987	24.243876845829817
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1680.0
1	13057.5
2	24435.0
3	80759.5
4	137084.0
5	83955.5
6	30827.0
7	27702.0
8	24577.0
9	24140.0
10	23703.0
11	23195.5
12	22688.0
13	21862.5
14	21037.0
15	19542.0
16	18047.0
17	17016.5
18	15986.0
19	14994.5
20	14003.0
21	13897.0
22	13791.0
23	14927.0
24	16063.0
25	16379.0
26	18499.0
27	20303.0
28	21978.5
29	23654.0
30	26990.0
31	30326.0
32	34829.0
33	39332.0
34	47998.5
35	56665.0
36	59746.5
37	62828.0
38	65622.0
39	68416.0
40	79478.0
41	90540.0
42	97984.5
43	105429.0
44	118229.5
45	131030.0
46	143094.0
47	155158.0
48	175506.5
49	195855.0
50	192223.5
51	188592.0
52	177218.0
53	165844.0
54	169533.0
55	173222.0
56	182152.0
57	191082.0
58	194559.5
59	198037.0
60	194244.0
61	190451.0
62	182629.0
63	174807.0
64	159479.0
65	144151.0
66	122058.0
67	99965.0
68	83185.0
69	66405.0
70	59087.0
71	51769.0
72	48506.5
73	45244.0
74	38027.0
75	24126.0
76	17442.0
77	12710.0
78	7978.0
79	5693.5
80	3409.0
81	2520.5
82	1632.0
83	1345.5
84	1059.0
85	646.5
86	234.0
87	179.0
88	124.0
89	79.0
90	34.0
91	27.5
92	21.0
93	19.0
94	17.0
95	11.5
96	6.0
97	4.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3112489.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.96559248569714
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.95410273499056	18.682162587349524
2	9.304382031602408	4.459700562012523
3	3.1076341122754423	2.2342887818833335
4	1.4342218280882122	1.374879034642147
5	0.8524075021502338	1.0214225414141753
6	0.5665470976780987	0.814658212014466
7	0.4402722620956402	0.7385969931298109
8	0.32386840068556005	0.6209358487859686
9	0.27010478384789105	0.582589906032228
>10	3.8556629574529127	23.735673570106147
>50	1.512187463356431	25.259026258302857
>100	0.36813668510999503	12.854315400784902
>500	0.007172699086770852	1.1668778904819936
>1k	0.002295263707766672	1.0938556271608257
>5k	5.73815926941668E-4	1.0021996224460268
>10k+	4.303619452062511E-4	4.358817163453037
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	88644	2.8480100652564557	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	27462	0.882316371238581	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	10679	0.3431016141743794	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9734	0.3127400610893725	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	8901	0.28597691429592204	No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	5361	0.17224157258065811	No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	5155	0.16562307529440265	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	4847	0.1557274579926226	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3593	0.11543815897823254	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.040032269993564636	0.0
2	0.0	0.0	0.0	0.1264904068737271	0.0
3	0.0	0.0	0.0	0.19855491858766408	0.0
4	0.0	0.0	0.0	0.24649083097161145	0.0
5	0.0	0.0	0.0	0.5292870111348185	0.0
6	0.0	0.0	0.0	0.6087411071974873	0.0
7	0.0	0.0	0.0	0.8442760761564138	0.0
8	0.0	0.0	0.0	1.1013693542370753	0.0
9	0.0	0.0	0.0	1.3240850007823322	0.0
10	0.0	0.0	0.0	1.4921819804021796	0.0
11	0.0	0.0	0.0	1.6825119703234293	0.0
12	0.0	0.0	0.0	1.84286595069091	0.0
13	0.0	0.0	0.0	1.9202959432145785	0.0
14	0.0	0.0	0.0	1.9815331074262432	0.0
15	0.0	0.0	0.0	2.1723771553891433	0.0
16	0.0	0.0	0.0	2.3903056364215263	0.0
17	0.0	0.0	0.0	2.6687644518583036	0.0
18	0.0	0.0	0.0	2.830981892626769	0.0
19	0.0	0.0	0.0	3.0177456048840656	0.0
20	0.0	0.0	0.0	3.229119845885399	0.0
21	0.0	0.0	0.0	3.445602538675639	0.0
22	0.0	0.0	0.0	3.65957919851283	0.0
23	0.0	0.0	0.0	3.8536361092360485	0.0
24	1.6064313801590946E-4	0.0	0.0	4.023949964160516	0.0
25	1.6064313801590946E-4	0.0	0.0	4.187805964936744	0.0
26	1.6064313801590946E-4	0.0	0.0	4.35962986535856	0.0
27	1.6064313801590946E-4	0.0	0.0	4.555261078834334	0.0
28	2.2490039322227323E-4	0.0	0.0	4.751824022510601	0.0
29	2.2490039322227323E-4	0.0	0.0	4.964804694892094	0.0
30	2.2490039322227323E-4	0.0	0.0	5.17277330136749	0.0
31	2.2490039322227323E-4	0.0	0.0	5.387167633363523	0.0
32	2.2490039322227323E-4	0.0	0.0	5.634525937280421	0.0
33	2.2490039322227323E-4	0.0	0.0	5.8619002348281395	0.0
34	2.2490039322227323E-4	0.0	0.0	6.142126124783092	0.0
35	2.2490039322227323E-4	0.0	0.0	6.386271565939671	0.0
36	2.2490039322227323E-4	0.0	0.0	6.648087752278	0.0
37	2.2490039322227323E-4	0.0	0.0	6.944827756820988	0.0
38	2.2490039322227323E-4	0.0	0.0	7.24343122176496	0.0
39	2.2490039322227323E-4	0.0	0.0	7.612267866649488	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACACCG	20	7.0351467E-4	45.0	19
CGTTTTT	25860	0.0	43.7732	1
TATACGG	170	0.0	42.352943	1
TACGGGA	625	0.0	41.4	3
CACTTAC	8685	0.0	41.243526	36
CGCACTT	8705	0.0	41.174614	34
ACTTACT	8665	0.0	40.975185	37
CCGCACT	8720	0.0	40.974773	33
CTTGCGG	910	0.0	40.796703	1
ACCCGCA	8820	0.0	40.688774	31
CTTACTG	8700	0.0	40.65517	38
CCCGCAC	8840	0.0	40.62217	32
GCACTTA	8875	0.0	40.385918	35
GCGCGAC	3265	0.0	39.96937	8
CATATGC	2875	0.0	39.83478	32
CGAATAT	260	0.0	39.80769	14
GCGATAT	85	0.0	39.705883	8
GGTACCT	2885	0.0	39.69671	7
ATCAACG	9470	0.0	39.654175	14
ACATACG	585	0.0	39.615383	16
>>END_MODULE