##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043426_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2889723
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.53213231856479	31.0	31.0	33.0	30.0	34.0
2	30.557945865399557	31.0	31.0	34.0	26.0	34.0
3	31.544095056861853	31.0	31.0	34.0	28.0	34.0
4	35.16689246685582	37.0	35.0	37.0	32.0	37.0
5	35.49818719648908	37.0	35.0	37.0	33.0	37.0
6	35.76570141844045	37.0	35.0	37.0	35.0	37.0
7	33.673759734064475	37.0	35.0	37.0	32.0	37.0
8	34.71132319602952	37.0	35.0	37.0	31.0	37.0
9	36.76467502248485	39.0	37.0	39.0	32.0	39.0
10	36.75630640030204	39.0	35.0	39.0	32.0	39.0
11	36.52004776928446	39.0	35.0	39.0	32.0	39.0
12	36.52042669833752	39.0	35.0	39.0	33.0	39.0
13	36.54213812188919	39.0	35.0	39.0	33.0	39.0
14	37.72284540767402	40.0	36.0	41.0	33.0	41.0
15	37.80769783124542	40.0	36.0	41.0	33.0	41.0
16	37.622241647382815	40.0	36.0	41.0	32.0	41.0
17	37.57230433505218	40.0	36.0	41.0	32.0	41.0
18	37.406751442958374	39.0	36.0	41.0	32.0	41.0
19	37.230037619522705	38.0	35.0	41.0	32.0	41.0
20	36.96729686547811	38.0	35.0	41.0	32.0	41.0
21	36.88796815473317	38.0	35.0	41.0	32.0	41.0
22	36.835778031319954	38.0	35.0	40.0	32.0	41.0
23	36.78368411089921	38.0	35.0	40.0	32.0	41.0
24	36.65074195692805	38.0	35.0	40.0	32.0	41.0
25	36.54137680324377	38.0	35.0	40.0	31.0	41.0
26	36.26952790976851	38.0	35.0	40.0	31.0	41.0
27	36.15208170471703	38.0	35.0	40.0	31.0	41.0
28	35.987604002182906	37.0	35.0	40.0	30.0	41.0
29	35.86095622313972	37.0	35.0	40.0	30.0	41.0
30	35.71301020893698	37.0	34.0	40.0	30.0	41.0
31	35.483134888707326	37.0	34.0	40.0	29.0	41.0
32	35.382398935814955	37.0	34.0	40.0	28.0	41.0
33	35.04673250688734	37.0	34.0	40.0	25.0	41.0
34	34.94365411494458	37.0	34.0	40.0	25.0	41.0
35	34.82276432723828	37.0	34.0	40.0	24.0	41.0
36	34.71775945306869	37.0	34.0	40.0	24.0	41.0
37	34.602445978386164	37.0	34.0	40.0	23.0	41.0
38	34.4955551102995	36.0	34.0	40.0	23.0	41.0
39	34.38588923575028	36.0	34.0	40.0	23.0	41.0
40	34.296017992035914	36.0	34.0	40.0	23.0	41.0
41	34.204910989738465	36.0	33.0	40.0	23.0	41.0
42	34.08339761285078	35.0	33.0	40.0	23.0	41.0
43	33.90648757683695	35.0	33.0	40.0	22.0	41.0
44	33.675076123213195	35.0	33.0	40.0	21.0	41.0
45	33.44070417822054	35.0	33.0	40.0	20.0	41.0
46	33.443528670395054	35.0	33.0	40.0	20.0	41.0
47	33.415000330481504	35.0	33.0	39.0	20.0	41.0
48	33.32951981902764	35.0	33.0	39.0	20.0	41.0
49	33.279350996618014	35.0	33.0	39.0	20.0	41.0
50	33.21576773967609	35.0	33.0	39.0	20.0	41.0
51	32.78610406602986	35.0	32.0	39.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	3.0
11	11.0
12	21.0
13	35.0
14	71.0
15	149.0
16	339.0
17	720.0
18	1583.0
19	2780.0
20	4745.0
21	7786.0
22	11949.0
23	18438.0
24	27650.0
25	41640.0
26	54401.0
27	63879.0
28	69103.0
29	74124.0
30	82605.0
31	94214.0
32	113792.0
33	150072.0
34	272242.0
35	292878.0
36	289804.0
37	312707.0
38	392360.0
39	509323.0
40	296.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.380982191026618	17.611203565186006	23.85412027381171	29.15369396997567
2	32.13404883443845	20.426594521343393	27.372415972049918	20.066940672168233
3	26.279473845763075	21.263525950411164	33.3648934517253	19.09210675210046
4	23.31586799149953	24.15383758235651	30.49157306773002	22.038721358413937
5	21.150885396281925	33.68385135876345	28.861313004741284	16.303950240213336
6	81.40146996788273	2.2611163768984084	14.902328008601518	1.43508564661734
7	77.07396176034865	7.594257304246808	13.84406048607427	1.4877204493302645
8	76.19425806556546	3.81912037935816	16.906880001993272	3.0797415530831156
9	44.161256978610055	18.286562414459794	23.0516558161457	14.500524790784445
10	30.288370200188737	18.386675816332566	30.097590668724994	21.227363314753696
11	27.07401366843812	15.53072733961006	38.4291850810614	18.966073910890422
12	22.415470271718085	17.54531489696417	38.70554374934898	21.333671081968756
13	18.62559144942266	21.305398475909282	40.71089166677913	19.35811840788892
14	19.532771826226945	19.118891326262066	41.43573622800524	19.912600619505742
15	24.45559660908675	15.957792494297895	39.73207812651939	19.854532770095958
16	23.258457644556245	16.850300184481352	36.27513779002347	23.616104380938935
17	23.806780096223758	20.273984738329588	36.100484371685454	19.8187507937612
18	24.47186114378437	24.371643925732673	32.00472848089592	19.151766449587036
19	26.539602584746014	21.01692792008092	31.67836501976141	20.765104475411658
20	25.799946915327176	23.251190512031776	33.93460203625053	17.014260536390513
21	22.746194012367273	19.13096860840987	35.46544080522597	22.657396573996884
22	24.424555571589387	20.91844097167791	34.30993212844276	20.347071328289942
23	25.533416178644114	19.984337599140126	35.01387503231278	19.468371189902978
24	26.307227370927937	18.016467322300443	32.751685888232196	22.924619418539425
25	20.438048906417674	20.268897745562462	34.258612330662835	25.03444101735703
26	18.637357283033705	20.879198456045785	35.8688704765128	24.61457378440771
27	20.311635405884925	22.84793386770981	35.40425847044855	21.436172255956713
28	23.341891247015717	23.4090257093846	32.721302353201324	20.52778069039835
29	21.996918043701765	20.63609557040588	37.20841063312989	20.158575752762463
30	28.03400187492019	20.612044822289192	32.08850813728513	19.265445165505483
31	27.032556407655683	22.016054826016195	31.61005397403142	19.341334792296703
32	27.4402079368853	20.73171027119208	30.014676147160124	21.81340564476249
33	22.201781970105785	22.40955274951959	34.14880941875744	21.239855861617187
34	21.954007356414436	24.424140306873703	32.12238681700634	21.49946551970552
35	25.211689840168074	27.42283602961253	28.298110234095102	19.067363896124302
36	24.445318807373578	25.61356226877109	27.849243681833862	22.091875242021466
37	23.042796835544447	29.659520999071535	27.13896799104966	20.158714174334357
38	23.551876771579835	24.90993081343783	27.5268944462843	24.011297968698038
39	22.23832526508596	21.99996331828345	31.90987509875514	23.85183631787545
40	19.679671719400094	24.101202779643586	32.05618670024774	24.16293880070858
41	19.87463850341365	26.528632675173363	28.067465289925714	25.529263531487274
42	19.3195333947233	21.61760833131757	29.71831556173377	29.34454271222536
43	20.8422745017429	21.418869559469886	32.94056212308239	24.798293815704827
44	24.15041164845212	23.463909862640815	29.948995111296135	22.436683377610933
45	24.258588106887753	24.429746380535438	30.508114445571426	20.803551067005387
46	21.692840455642287	24.351953457130666	29.983600504269788	23.97160558295726
47	20.389670567040508	25.5665335397199	32.371234197879865	21.67256169535973
48	23.149658289047082	24.3587361141535	31.521014297910217	20.9705912988892
49	21.05035672969347	22.323558347980065	33.593877337031955	23.032207585294508
50	19.966654243330588	22.741418468136914	35.26164964600413	22.030277642528368
51	19.543015022547145	21.096935588636004	33.155011743340104	26.205037645476747
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1520.0
1	13468.0
2	25416.0
3	93518.5
4	161621.0
5	97678.0
6	33735.0
7	30090.5
8	26446.0
9	26611.5
10	26777.0
11	27026.5
12	27276.0
13	26068.5
14	24861.0
15	23077.5
16	21294.0
17	19289.0
18	17284.0
19	16077.0
20	14870.0
21	14214.0
22	13558.0
23	13500.0
24	13442.0
25	14230.0
26	16226.5
27	17435.0
28	18806.0
29	20177.0
30	23393.0
31	26609.0
32	30091.5
33	33574.0
34	39345.0
35	45116.0
36	50249.0
37	55382.0
38	58982.0
39	62582.0
40	68515.5
41	74449.0
42	83705.5
43	92962.0
44	99702.0
45	106442.0
46	117516.5
47	128591.0
48	147183.5
49	165776.0
50	169746.5
51	173717.0
52	167310.0
53	160903.0
54	162529.0
55	164155.0
56	169297.5
57	174440.0
58	177463.5
59	180487.0
60	181364.0
61	182241.0
62	177247.5
63	172254.0
64	156755.0
65	141256.0
66	117949.0
67	94642.0
68	77345.0
69	60048.0
70	52349.5
71	44651.0
72	41383.5
73	38116.0
74	32444.5
75	19638.5
76	12504.0
77	9185.5
78	5867.0
79	4490.0
80	3113.0
81	2238.0
82	1363.0
83	953.5
84	544.0
85	415.5
86	287.0
87	180.0
88	73.0
89	56.0
90	39.0
91	28.0
92	17.0
93	13.5
94	10.0
95	9.5
96	9.0
97	5.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2889723.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.067484272305485
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.07052583984353	20.090347201884352
2	8.670404974791566	4.520312906297968
3	2.8774100211845917	2.2502052141661064
4	1.4817866338070813	1.5450579908671431
5	0.9299068549559151	1.212016615813619
6	0.7191282730665658	1.1247518968759747
7	0.557844067605576	1.017911402109509
8	0.4839614981225408	1.0092526992568587
9	0.3946082162455167	0.9257799123632275
>10	5.6528919871793235	34.84069508253721
>50	1.0144120525297118	17.44033191952798
>100	0.1377757562382932	5.580868943330099
>500	0.005804496994259977	0.9877501443563199
>1k	0.002831461948419501	1.466069950857432
>5k	1.4157309742097502E-4	0.322324295191784
>10k+	5.662923896839001E-4	5.66632382456452
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	105089	3.636646142208094	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	26026	0.9006399575322618	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	11260	0.38965672488331926	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11165	0.38636921255082235	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	8734	0.30224350223187485	No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	4854	0.16797457749410585	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4377	0.15146780504567392	No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	4072	0.1409131601887101	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	3689	0.1276592946798015	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.038135143056964284	0.0
2	0.0	0.0	0.0	0.12163795630238608	0.0
3	0.0	0.0	0.0	0.19860035027578768	0.0
4	0.0	0.0	0.0	0.25050843973626535	0.0
5	0.0	0.0	0.0	0.5396711034240999	0.0
6	0.0	0.0	0.0	0.6549070620263603	0.0
7	0.0	0.0	0.0	0.9521327822770557	0.0
8	0.0	0.0	0.0	1.2901582608436863	0.0
9	3.46053929736518E-5	0.0	0.0	1.592436368468535	0.0
10	3.46053929736518E-5	0.0	0.0	1.7875761794469573	0.0
11	3.46053929736518E-5	0.0	0.0	1.9748605662203609	0.0
12	3.46053929736518E-5	0.0	0.0	2.1231792805054326	0.0
13	3.46053929736518E-5	0.0	0.0	2.2080317040768267	0.0
14	3.46053929736518E-5	0.0	0.0	2.26814127167206	0.0
15	3.46053929736518E-5	0.0	0.0	2.4299906946098293	0.0
16	3.46053929736518E-5	0.0	0.0	2.6086583385327935	0.0
17	3.46053929736518E-5	0.0	0.0	2.8513459594570136	0.0
18	3.46053929736518E-5	0.0	0.0	2.988660158776464	0.0
19	3.46053929736518E-5	0.0	0.0	3.1445228487297916	0.0
20	3.46053929736518E-5	0.0	0.0	3.33412579683243	0.0
21	3.46053929736518E-5	0.0	0.0	3.5314803529611662	0.0
22	3.46053929736518E-5	0.0	0.0	3.726412531581747	0.0
23	3.46053929736518E-5	0.0	0.0	3.900200815095426	0.0
24	6.92107859473036E-5	0.0	0.0	4.049419269597813	0.0
25	6.92107859473036E-5	0.0	0.0	4.204659062477615	0.0
26	6.92107859473036E-5	0.0	0.0	4.3627711029742295	0.0
27	6.92107859473036E-5	0.0	0.0	4.539708477248511	0.0
28	6.92107859473036E-5	0.0	0.0	4.710416880787536	0.0
29	1.038161789209554E-4	0.0	0.0	4.8994315372096215	0.0
30	1.038161789209554E-4	0.0	0.0	5.089069090705234	0.0
31	1.038161789209554E-4	0.0	0.0	5.282928502143631	0.0
32	1.384215718946072E-4	0.0	0.0	5.505510389750159	0.0
33	1.384215718946072E-4	0.0	0.0	5.72622358613611	0.0
34	1.384215718946072E-4	0.0	0.0	5.982822575035739	0.0
35	1.384215718946072E-4	0.0	0.0	6.210664482374262	0.0
36	1.384215718946072E-4	0.0	0.0	6.457331723490452	0.0
37	1.384215718946072E-4	0.0	0.0	6.7869480915644855	0.0
38	1.384215718946072E-4	0.0	0.0	7.113311552698995	0.0
39	1.384215718946072E-4	0.0	0.0	7.500580505467133	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	29085	0.0	43.72357	1
CGCACTT	8165	0.0	41.748314	34
CACTTAC	8215	0.0	41.65855	36
ACCCGCA	8320	0.0	41.29507	31
ACTTACT	8205	0.0	41.270565	37
CTTACTG	8180	0.0	41.204155	38
CCCGCAC	8355	0.0	41.17594	32
CCGCACT	8315	0.0	41.02225	33
GCACTTA	8345	0.0	40.9287	35
CACGACG	660	0.0	40.90909	25
ATAGCGG	335	0.0	40.298508	1
TATGACC	8660	0.0	40.141453	27
TACGGGA	545	0.0	40.04587	3
TTATGAC	8740	0.0	40.005722	26
GCTTATG	8785	0.0	39.903244	24
ATCAACG	8880	0.0	39.83108	14
CTTATGA	8780	0.0	39.823463	25
AACGCAA	8875	0.0	39.777466	17
AGCTTAT	8825	0.0	39.77337	23
CGGTCTA	680	0.0	39.705883	30
>>END_MODULE