##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043425_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	906367
Sequences flagged as poor quality	0
Sequence length	51
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.467732165888652	31.0	31.0	33.0	30.0	34.0
2	30.512213043943568	31.0	31.0	34.0	26.0	34.0
3	31.377168409706	31.0	31.0	34.0	28.0	34.0
4	35.011841781529995	37.0	35.0	37.0	32.0	37.0
5	35.3155101631017	37.0	35.0	37.0	33.0	37.0
6	35.79486565596497	37.0	35.0	37.0	35.0	37.0
7	33.80937302439299	37.0	35.0	37.0	32.0	37.0
8	34.79390467658244	37.0	35.0	37.0	31.0	37.0
9	36.7277317025002	39.0	37.0	39.0	32.0	39.0
10	36.683539890574124	39.0	35.0	39.0	32.0	39.0
11	36.40196079513045	38.0	35.0	39.0	32.0	39.0
12	36.27484010340182	39.0	35.0	39.0	32.0	39.0
13	36.32147684105887	39.0	35.0	39.0	32.0	39.0
14	37.68650226674184	40.0	36.0	41.0	33.0	41.0
15	37.8296164798586	40.0	36.0	41.0	33.0	41.0
16	37.802144164560275	40.0	36.0	41.0	33.0	41.0
17	37.7524380300695	40.0	36.0	41.0	33.0	41.0
18	37.44265843747621	39.0	36.0	41.0	33.0	41.0
19	37.09225843394563	38.0	35.0	40.0	33.0	41.0
20	36.75135458373926	38.0	35.0	40.0	32.0	41.0
21	36.63837827281885	38.0	35.0	40.0	32.0	41.0
22	36.55316113671394	37.0	35.0	40.0	32.0	41.0
23	36.44444358631768	37.0	35.0	40.0	32.0	41.0
24	36.23393614286486	37.0	35.0	40.0	32.0	41.0
25	36.12037176993425	36.0	35.0	40.0	31.0	41.0
26	35.86449970045246	36.0	35.0	40.0	31.0	41.0
27	35.76558060917928	36.0	35.0	40.0	31.0	41.0
28	35.64796820713905	36.0	35.0	40.0	31.0	41.0
29	35.56909176966946	36.0	35.0	40.0	31.0	41.0
30	35.366748789397676	35.0	35.0	40.0	30.0	41.0
31	34.8948152348883	35.0	34.0	40.0	27.0	41.0
32	34.713470371273445	35.0	34.0	40.0	25.0	41.0
33	34.54235204944575	35.0	34.0	40.0	25.0	41.0
34	34.355598780626394	35.0	34.0	40.0	23.0	41.0
35	34.1183527202557	35.0	34.0	40.0	22.0	41.0
36	33.889765404080244	35.0	33.0	40.0	21.0	41.0
37	33.8213339629532	35.0	33.0	40.0	21.0	41.0
38	33.71270136710626	35.0	33.0	40.0	21.0	41.0
39	33.53975486750952	35.0	33.0	40.0	20.0	41.0
40	33.47875970771222	35.0	33.0	40.0	20.0	41.0
41	33.41879724217674	35.0	33.0	40.0	20.0	41.0
42	33.33050519270891	35.0	33.0	40.0	18.0	41.0
43	33.16463088351628	35.0	33.0	40.0	18.0	41.0
44	32.96253504375159	35.0	33.0	39.0	18.0	41.0
45	32.80517494568977	35.0	33.0	39.0	18.0	41.0
46	32.80743120612291	35.0	33.0	39.0	18.0	41.0
47	32.76936715480595	35.0	33.0	39.0	18.0	41.0
48	32.67832897711413	35.0	33.0	39.0	18.0	41.0
49	32.601504688498146	35.0	33.0	39.0	17.0	41.0
50	32.47719301342613	35.0	32.0	39.0	15.0	41.0
51	32.06653154847871	35.0	31.0	38.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	6.0
12	7.0
13	9.0
14	24.0
15	45.0
16	118.0
17	294.0
18	547.0
19	1136.0
20	1779.0
21	3003.0
22	4706.0
23	7073.0
24	11206.0
25	16582.0
26	20794.0
27	22456.0
28	22743.0
29	23420.0
30	25882.0
31	30190.0
32	36443.0
33	47554.0
34	80212.0
35	117465.0
36	114068.0
37	80165.0
38	97843.0
39	140528.0
40	68.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.702465998872423	17.66160948048638	25.4776486787361	32.1582758419051
2	33.77086765074192	20.072774052894687	28.94875916709236	17.207599129271035
3	20.15541165995673	21.438225354630077	40.551895644920876	17.854467340492317
4	21.70489437501586	24.092668863716355	37.65373187682252	16.548704884445264
5	18.51733348632508	29.75726168318132	36.105352467598664	15.620052362894942
6	74.95915010144897	2.045639349181954	21.31178650590765	1.6834240434614234
7	71.9168945912638	7.155489994671033	19.236247568589764	1.691367845475398
8	70.72433131391588	3.822844388641687	22.59978573800679	2.853038559435637
9	39.14661500253209	21.878885705238606	28.310607071969745	10.663892220259562
10	28.7413376700608	24.143972585056606	36.373014463236196	10.741675281646398
11	26.38224913307744	21.662417100357803	39.049634419611486	12.905699346953275
12	17.697025597798685	30.695512965498523	37.690471961137156	13.91698947556564
13	12.99881835945042	33.38106969913953	37.792086428565916	15.828025512844135
14	12.454447260326114	25.697868523456833	46.303208303038396	15.544475913178657
15	13.73571632683008	23.502289911261112	46.606396746571754	16.155597015337055
16	15.567645335719416	24.959646589074843	36.85615208850278	22.616555986702956
17	16.87285613884883	25.011612293916265	41.17912501227428	16.93640655496063
18	20.203405463791157	27.353047937535237	36.79326365589215	15.650282942781457
19	20.073656697562907	26.636009475190512	37.24539838718753	16.04493544005905
20	16.2692375163703	30.00793276895562	38.0543422256106	15.668487489063482
21	16.29328958357928	27.31134297696187	36.26047726803822	20.13489017142063
22	13.680551035066369	30.276146417510784	36.942430604821226	19.100871942601614
23	13.278285727525386	27.062768172274588	44.74776773646878	14.911178363731247
24	13.985284106769114	27.82901407487254	42.223403985361344	15.962297832997008
25	13.284353909619393	31.936732030182036	37.79517568490468	16.983738375293893
26	13.43649978430371	32.56043081886256	38.0064587523597	15.996610644474037
27	13.942586170943999	29.819267471123727	41.68763867175217	14.5505076861801
28	13.364012590926192	27.232235948572708	40.74221590150568	18.661535558995418
29	15.329220944716655	30.840928674587666	38.53913480963009	15.290715571065583
30	19.281262446668954	29.771494328456356	35.83206361220124	15.115179612673455
31	19.54649716946888	28.292402525687717	36.7798033247018	15.381296980141599
32	18.713721925003888	30.6157439536082	34.49750487385353	16.173029247534387
33	15.254306478501533	30.812573714621116	35.076961098539556	18.85615870833779
34	16.4285548789839	28.964757101703835	37.324174423826115	17.282513595486154
35	21.168025755571417	29.258126123303253	34.35208916476438	15.221758956360945
36	16.87351812235	32.23374196103786	35.71853344175152	15.174206474860624
37	17.44381690860325	31.995207239451567	34.74828629021136	15.812689561733823
38	17.3164954152126	32.17890766102473	33.77086765074192	16.733729273020753
39	19.47147237267023	28.969170325044928	34.674806121582094	16.88455118070274
40	15.300314331832471	29.287363727938022	35.60169335379598	19.81062858643353
41	17.191049541741922	29.14073438243008	32.95883455598008	20.70938151984792
42	16.71751067724222	28.775540150954303	35.09450366132042	19.41244551048306
43	16.828834236021393	30.370810058177316	34.967954482014456	17.83240122378683
44	14.813977119643587	35.10299911625203	32.732325867998284	17.3506978961061
45	17.345512358680317	32.346058495068775	33.692863928188025	16.61556521806288
46	17.332162358073496	30.692092717409174	33.190087459053565	18.78565746546377
47	16.08432345837834	30.064753019472246	36.0568070108466	17.794116511302814
48	16.775544564177647	28.728318661204565	36.32733760165584	18.168799172961947
49	17.38324541824669	30.460839814335692	34.54582966943854	17.610085097979074
50	15.788747825108373	30.78157082064991	34.28743544281731	19.142245911424403
51	15.09311349596797	28.151951692857306	36.41129917572022	20.34363563545451
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1235.0
1	6599.5
2	11964.0
3	47874.5
4	83785.0
5	48594.5
6	13404.0
7	13498.0
8	13592.0
9	16289.0
10	18986.0
11	21727.0
12	24468.0
13	23769.5
14	23071.0
15	20463.5
16	17856.0
17	15250.5
18	12645.0
19	10954.5
20	9264.0
21	8589.0
22	7914.0
23	7190.5
24	6467.0
25	6296.5
26	6718.5
27	7311.0
28	8210.0
29	9109.0
30	10469.5
31	11830.0
32	13244.0
33	14658.0
34	16991.5
35	19325.0
36	22421.5
37	25518.0
38	27004.0
39	28490.0
40	32950.0
41	37410.0
42	45456.5
43	53503.0
44	56957.0
45	60411.0
46	60898.5
47	61386.0
48	61842.0
49	62298.0
50	62760.5
51	63223.0
52	59521.0
53	55819.0
54	48593.0
55	41367.0
56	36785.0
57	32203.0
58	27314.0
59	22425.0
60	18799.0
61	15173.0
62	12778.0
63	10383.0
64	8617.0
65	6851.0
66	5563.0
67	4275.0
68	3897.0
69	3519.0
70	3144.5
71	2770.0
72	2711.0
73	2652.0
74	2190.5
75	1336.0
76	943.0
77	741.0
78	539.0
79	397.0
80	255.0
81	193.5
82	132.0
83	81.0
84	30.0
85	25.5
86	21.0
87	15.5
88	10.0
89	9.5
90	9.0
91	7.5
92	6.0
93	6.0
94	6.0
95	3.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	906367.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.08630467977497
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.91901077299244	27.68976457007656
2	9.147735796389249	6.419204905643943
3	3.379421594857922	3.5571424715578828
4	1.790168516419451	2.5124159198093436
5	1.183267104750449	2.075823507741473
6	0.7961190922745667	1.6759726219758797
7	0.5816359704593924	1.4285219810578387
8	0.4196453352583615	1.177904327225695
9	0.33571457412386635	1.0601085449835785
>10	2.752294743505425	21.19302888697098
>50	0.5494106115587309	13.26030460819361
>100	0.14036511739101312	7.475553778415374
>500	0.0026053850095778567	0.6924559918253159
>1k	0.0019540387571833928	1.0488537209512498
>5k	3.256731261972321E-4	0.8008950572118216
>10k+	3.256731261972321E-4	7.932049106359399
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	69417	7.658818116723137	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7009	0.7733070599437094	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2847	0.3141111713025739	No Hit
GTAATGGGTTTACCTGGCTTCCTTCTTGGTACATAGGCTACTCTTCCACAC	1765	0.19473347992590198	No Hit
TTAGGCGGGATAAAATTTCAATTAATTCCATAATTTACACCAACTTCCTAA	1274	0.14056116341393718	No Hit
CTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1219	0.1344929813199289	No Hit
CCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGC	1040	0.11474380686852013	No Hit
CTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGCT	1034	0.1140818233673556	Illumina Single End Adapter 1 (95% over 21bp)
GCGAAGGGTCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCACAAGCCA	949	0.10470372376752463	No Hit
GTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATT	929	0.10249711209697618	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.12312893121660431	0.0
2	0.0	0.0	0.0	0.40270662987509476	0.0
3	0.0	0.0	0.0	0.6373797810379239	0.0
4	0.0	0.0	0.0	0.7936078873127552	0.0
5	0.0	0.0	0.0	1.7000839615740644	0.0
6	0.0	0.0	0.0	1.9499827332636779	0.0
7	0.0	0.0	0.0	2.697693097829025	0.0
8	0.0	0.0	0.0	3.571069997032107	0.0
9	0.0	0.0	0.0	4.2323915146954825	0.0
10	0.0	0.0	0.0	4.683864262489698	0.0
11	0.0	0.0	0.0	5.182006847116014	0.0
12	0.0	0.0	0.0	5.59276760958861	0.0
13	0.0	0.0	0.0	5.7584841460468	0.0
14	0.0	0.0	0.0	5.899376301211319	0.0
15	0.0	0.0	0.0	6.367177975367594	0.0
16	0.0	0.0	0.0	6.909894115738989	0.0
17	0.0	0.0	0.0	7.590082163185553	0.0
18	0.0	0.0	0.0	7.9078342437445315	0.0
19	0.0	0.0	0.0	8.299176823516301	0.0
20	0.0	0.0	0.0	8.72185328900986	0.0
21	0.0	0.0	0.0	9.131731406814238	0.0
22	1.1033058352742322E-4	0.0	0.0	9.546574400877349	0.0
23	1.1033058352742322E-4	0.0	0.0	9.868850035360952	0.0
24	6.619835011645393E-4	0.0	0.0	10.155047569031089	0.0
25	6.619835011645393E-4	0.0	0.0	10.437604193444818	0.0
26	6.619835011645393E-4	0.0	0.0	10.754142637584996	0.0
27	6.619835011645393E-4	0.0	0.0	11.056558767033662	0.0
28	6.619835011645393E-4	0.0	0.0	11.364050103324592	0.0
29	6.619835011645393E-4	0.0	0.0	11.743366649491872	0.0
30	6.619835011645393E-4	0.0	0.0	12.092342285189112	0.0
31	6.619835011645393E-4	0.0	0.0	12.41185965508453	0.0
32	6.619835011645393E-4	0.0	0.0	12.737003884739845	0.0
33	6.619835011645393E-4	0.0	0.0	13.078918363091331	0.0
34	6.619835011645393E-4	0.0	0.0	13.483941935220502	0.0
35	6.619835011645393E-4	0.0	0.0	13.832917570917742	0.0
36	6.619835011645393E-4	0.0	0.0	14.171301470596347	0.0
37	6.619835011645393E-4	0.0	0.0	14.535944049154482	0.0
38	6.619835011645393E-4	0.0	0.0	14.899372991293813	0.0
39	6.619835011645393E-4	0.0	0.0	15.276372595207018	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAGGCG	145	0.0	45.000004	1
CGGTTAC	35	1.2115561E-7	45.000004	19
TAAACGG	90	0.0	45.000004	1
CATCGAA	35	1.2115561E-7	45.000004	13
ACGTAGG	55	1.8189894E-12	45.000004	1
TATCGTC	35	1.2115561E-7	45.000004	11
TACCGTA	35	1.2115561E-7	45.000004	23
CAGCGCG	20	7.0324965E-4	45.0	27
TCGTTAC	20	7.0324965E-4	45.0	33
CACGCGT	20	7.0324965E-4	45.0	14
TCGGGCA	50	2.1827873E-11	45.0	4
GCACGAT	20	7.0324965E-4	45.0	40
CACGACC	20	7.0324965E-4	45.0	26
CGACAAG	20	7.0324965E-4	45.0	27
AACGGCC	25	3.8901508E-5	45.0	5
AGGCCGC	20	7.0324965E-4	45.0	35
GTTCGCG	20	7.0324965E-4	45.0	33
CTTGCGG	190	0.0	45.0	1
GCGATAA	50	2.1827873E-11	45.0	8
CCGGTTA	20	7.0324965E-4	45.0	29
>>END_MODULE