##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043424_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3765191
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.551293413800256	31.0	31.0	33.0	30.0	34.0
2	30.603837361769962	31.0	31.0	34.0	26.0	34.0
3	31.56943751326294	31.0	31.0	34.0	28.0	34.0
4	35.19067000850687	37.0	35.0	37.0	33.0	37.0
5	35.51030744522655	37.0	35.0	37.0	33.0	37.0
6	35.779363118630634	37.0	35.0	37.0	35.0	37.0
7	33.784245739459166	37.0	35.0	37.0	32.0	37.0
8	34.769383014035675	37.0	35.0	37.0	32.0	37.0
9	36.79619440288687	39.0	37.0	39.0	32.0	39.0
10	36.757845219538666	39.0	35.0	39.0	32.0	39.0
11	36.51653262742846	39.0	35.0	39.0	33.0	39.0
12	36.51565909936574	39.0	35.0	39.0	33.0	39.0
13	36.54494977811218	39.0	35.0	39.0	33.0	39.0
14	37.73983497782715	40.0	36.0	41.0	33.0	41.0
15	37.79292630838648	40.0	36.0	41.0	33.0	41.0
16	37.56694653737354	40.0	35.0	41.0	32.0	41.0
17	37.51335270906576	40.0	36.0	41.0	32.0	41.0
18	37.37955657495198	39.0	35.0	41.0	32.0	41.0
19	37.23132080152109	39.0	35.0	41.0	32.0	41.0
20	36.99421861998501	38.0	35.0	41.0	32.0	41.0
21	36.900509429667714	38.0	35.0	41.0	32.0	41.0
22	36.848810325956904	38.0	35.0	40.0	32.0	41.0
23	36.80431457527653	38.0	35.0	40.0	32.0	41.0
24	36.67823677470811	38.0	35.0	40.0	32.0	41.0
25	36.563991308807445	38.0	35.0	40.0	31.0	41.0
26	36.300953922390654	38.0	35.0	40.0	31.0	41.0
27	36.192482931144795	38.0	35.0	40.0	31.0	41.0
28	36.02046669080001	37.0	35.0	40.0	30.0	41.0
29	35.87823247213753	37.0	35.0	40.0	30.0	41.0
30	35.70577641346747	37.0	34.0	40.0	30.0	41.0
31	35.562335084727444	37.0	34.0	40.0	29.0	41.0
32	35.468068153780244	37.0	34.0	40.0	28.0	41.0
33	35.120836101010546	37.0	34.0	40.0	25.0	41.0
34	34.998375912403915	37.0	34.0	40.0	25.0	41.0
35	34.9154478484624	37.0	34.0	40.0	25.0	41.0
36	34.82105715221353	37.0	34.0	40.0	24.0	41.0
37	34.705900444359926	37.0	34.0	40.0	24.0	41.0
38	34.58100717865309	37.0	34.0	40.0	23.0	41.0
39	34.458595593158485	36.0	34.0	40.0	23.0	41.0
40	34.373480920357025	36.0	34.0	40.0	23.0	41.0
41	34.2618095602587	36.0	33.0	40.0	23.0	41.0
42	34.138267886011626	35.0	33.0	40.0	23.0	41.0
43	33.95125081303976	35.0	33.0	40.0	23.0	41.0
44	33.721838546836004	35.0	33.0	40.0	22.0	41.0
45	33.511413630809166	35.0	33.0	40.0	20.0	41.0
46	33.514063695573476	35.0	33.0	40.0	22.0	41.0
47	33.4891677473998	35.0	33.0	39.0	22.0	41.0
48	33.398008494124205	35.0	33.0	39.0	22.0	41.0
49	33.35199568893052	35.0	33.0	39.0	22.0	41.0
50	33.29039190840518	35.0	33.0	39.0	21.0	41.0
51	32.847907051727255	35.0	32.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	0.0
9	0.0
10	3.0
11	7.0
12	25.0
13	37.0
14	91.0
15	187.0
16	416.0
17	901.0
18	1820.0
19	3463.0
20	6094.0
21	9626.0
22	14930.0
23	22284.0
24	34297.0
25	50914.0
26	68109.0
27	81765.0
28	90146.0
29	96591.0
30	107375.0
31	124180.0
32	148411.0
33	198940.0
34	366246.0
35	366830.0
36	370228.0
37	418945.0
38	524906.0
39	657032.0
40	390.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.758065394292082	17.33564114011746	23.99004459534722	28.916248870243237
2	32.044562945146744	20.34709527351999	26.964236342857507	20.644105438475762
3	26.642685590186527	21.506053743355917	32.56145571366765	19.289804952789915
4	23.92850721251591	24.143078000558273	29.655361441159293	22.27305334576652
5	22.089901946541357	33.2881120771828	28.108295170152058	16.51369080612378
6	82.13809073696395	2.299963003204884	14.105818270573788	1.4561279892573842
7	77.78439393911225	7.36241534625999	13.266896685984856	1.5862940286429028
8	76.42589711916341	3.9238115675937824	16.39914150437521	3.2511498088675976
9	45.15558440461586	17.597540204467716	22.6549728818538	14.59190250906262
10	31.14800816213573	17.969048582130362	29.61677110138636	21.266172154347547
11	27.270754657599046	15.354626099977398	38.03299752920901	19.34162171321455
12	22.567381043883298	16.672354735788968	38.53889483959777	22.221369380729953
13	19.174538555945766	19.69884130712094	41.27764036406121	19.84897977287208
14	20.286195308551413	18.157671151343983	41.31450436378925	20.24162917631536
15	25.463993725683505	14.972148823260229	39.21113165308214	20.352725797974127
16	24.643902527122794	15.865383721569504	36.190886464989426	23.29982728631828
17	24.537984925598728	19.484004928302443	35.6787477713614	20.29926237473743
18	25.411592665551364	23.123714042660783	31.78943644558802	19.675256846199833
19	26.586220991179466	20.951075257536736	31.155630617410914	21.30707313387289
20	26.777950972473906	22.469962347195665	33.54685061129701	17.20523606903342
21	23.011980003139282	18.57810134997136	35.34471425221191	23.065204394677455
22	25.50417761011327	19.382310220118978	34.14929016881215	20.964222000955594
23	26.741644713375763	18.894021578188198	34.10193533342664	20.262398375009393
24	27.005084204227618	17.127763239633794	32.685247574425844	23.181904981712748
25	21.248722840355246	19.091302406703935	34.48542185509314	25.17455289784768
26	19.640305100059997	19.350492445137576	36.05766613167831	24.951536323124113
27	21.21709097891714	22.060155779613837	34.46717576877242	22.255577472696604
28	24.649187783567953	22.464331822741528	32.19164711697229	20.69483327671823
29	23.540877474741652	19.13963992796116	37.018068937273036	20.301413660024153
30	28.407748770248308	19.253073748449946	32.63138576502493	19.70779171627681
31	29.07082270195589	19.686624131418565	31.1295496031941	20.113003563431445
32	29.21620709281415	19.0197522516122	29.97839950217665	21.785641153397
33	24.182836939746217	21.472961132649047	33.64023232818734	20.7039695994174
34	23.247080958177154	23.754173427058547	31.997234668838846	21.001510945925453
35	26.494671850644497	26.298400267078087	28.065667850581814	19.1412600316956
36	25.992200661267916	24.472755830979093	27.778059599101347	21.756983908651645
37	25.44707559324348	27.718275115392554	26.75056856345402	20.084080727909953
38	25.15481950318058	23.74485119081608	26.969866867311644	24.130462438691687
39	23.77759853351397	21.035347210805508	31.566233957321156	23.620820298359366
40	21.755177891373904	22.57433952221813	31.120625753115842	24.54985683329212
41	21.6748632406696	25.51041899335253	27.901957696169994	24.91276006980788
42	20.913042658393692	20.605329185159533	29.535101937723745	28.946526218723033
43	22.203282648874918	19.809008361063224	32.88507807439251	25.102630915669348
44	25.189611894854735	21.974024690912096	30.22547860121837	22.6108848130148
45	25.390451639770735	23.08740778356264	30.87261708635764	20.64952349030899
46	22.93224965214248	22.89934295497891	30.279606001395415	23.888801391483195
47	21.835094155914003	24.15627786213236	32.349806424162814	21.658821557790827
48	24.466992511136883	23.114205892875024	31.085514652510327	21.333286943477766
49	22.446059177343194	21.13648417835908	32.81504178672476	23.602414857572963
50	21.232893630097387	21.901040345629212	35.03588529771797	21.83018072655544
51	20.81920943718393	19.98233290157126	33.44130483685954	25.757152824385273
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1634.0
1	16197.0
2	30760.0
3	112828.0
4	194896.0
5	118425.0
6	41954.0
7	36520.5
8	31087.0
9	30218.5
10	29350.0
11	28706.5
12	28063.0
13	26765.0
14	25467.0
15	23938.0
16	22409.0
17	20778.0
18	19147.0
19	18178.5
20	17210.0
21	17098.5
22	16987.0
23	17584.0
24	18181.0
25	19584.5
26	22872.5
27	24757.0
28	26139.0
29	27521.0
30	32767.5
31	38014.0
32	39795.5
33	41577.0
34	49694.0
35	57811.0
36	60829.5
37	63848.0
38	69891.0
39	75934.0
40	82146.0
41	88358.0
42	100639.0
43	112920.0
44	124936.5
45	136953.0
46	148042.0
47	159131.0
48	180778.0
49	202425.0
50	208813.5
51	215202.0
52	209073.0
53	202944.0
54	204353.0
55	205762.0
56	216391.0
57	227020.0
58	231656.5
59	236293.0
60	241142.5
61	245992.0
62	246837.0
63	247682.0
64	228248.5
65	208815.0
66	176019.0
67	143223.0
68	118557.5
69	93892.0
70	81527.5
71	69163.0
72	64560.5
73	59958.0
74	50304.5
75	30859.0
76	21067.0
77	15809.5
78	10552.0
79	7921.0
80	5290.0
81	3965.5
82	2641.0
83	1791.0
84	941.0
85	605.5
86	270.0
87	257.5
88	245.0
89	162.0
90	79.0
91	74.0
92	69.0
93	55.0
94	41.0
95	25.5
96	10.0
97	8.5
98	7.0
99	3.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3765191.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.98349440413183
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.19410482325043	18.513843810603923
2	8.738595606812751	4.191641176719291
3	2.7991679211781815	2.0140148452140667
4	1.3837228158313948	1.3274603364144717
5	0.8626743984949352	1.0344973304445533
6	0.6479473437466448	0.9324024895751845
7	0.4945959439885057	0.8303497338468238
8	0.4106362775702666	0.787879429219201
9	0.370899229853553	0.8005913643320542
>10	5.562129588657271	32.60636744156553
>50	1.306635063583159	21.079788818393805
>100	0.2188557528170508	7.878185668209656
>500	0.006257263687505625	1.0535553919983303
>1k	0.0028334778962286716	1.1922436931702414
>5k	4.722463160381119E-4	0.7170564595507338
>10k+	4.722463160381119E-4	5.040122010742008
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	121073	3.215587203942642	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	32249	0.8565036939693099	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12623	0.3352552367197308	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	12055	0.32016968063505946	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	8976	0.23839428066199034	No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	5996	0.15924822937269317	No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	5220	0.1386383851443393	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5132	0.13630118631431978	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	4271	0.11343382048878796	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	4145	0.11008737670944184	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.030967884497758547	0.0
2	0.0	0.0	0.0	0.10036675430276977	0.0
3	0.0	0.0	0.0	0.16015123801156436	0.0
4	0.0	0.0	0.0	0.20065383137269796	0.0
5	0.0	0.0	0.0	0.4410666019333415	0.0
6	0.0	0.0	0.0	0.5433987279795368	0.0
7	0.0	0.0	0.0	0.8304492388301151	0.0
8	0.0	0.0	0.0	1.1642437262810839	0.0
9	0.0	0.0	0.0	1.4960994010662407	0.0
10	0.0	0.0	0.0	1.688280886680118	0.0
11	0.0	0.0	0.0	1.864022303251017	0.0
12	0.0	0.0	0.0	2.007361645133009	0.0
13	0.0	0.0	0.0	2.0819926532279505	0.0
14	0.0	0.0	0.0	2.13864316577831	0.0
15	0.0	0.0	0.0	2.283283902463381	0.0
16	0.0	0.0	0.0	2.443647613095856	0.0
17	0.0	0.0	0.0	2.6644332252998586	0.0
18	0.0	0.0	0.0	2.7928198064852485	0.0
19	0.0	0.0	0.0	2.944976762135042	0.0
20	0.0	0.0	0.0	3.1137331412934963	0.0
21	0.0	0.0	0.0	3.2908025117450883	0.0
22	5.31181552277162E-5	0.0	0.0	3.4666501646264427	0.0
23	5.31181552277162E-5	0.0	0.0	3.627730970354492	0.0
24	7.96772328415743E-5	0.0	0.0	3.7669270961287222	0.0
25	7.96772328415743E-5	0.0	0.0	3.911674069124249	0.0
26	7.96772328415743E-5	0.0	0.0	4.062343716427666	0.0
27	7.96772328415743E-5	0.0	0.0	4.228258274281438	0.0
28	7.96772328415743E-5	0.0	0.0	4.3960054084905655	0.0
29	7.96772328415743E-5	0.0	0.0	4.570232957637474	0.0
30	7.96772328415743E-5	0.0	0.0	4.748683400124987	0.0
31	7.96772328415743E-5	0.0	0.0	4.939961877099993	0.0
32	7.96772328415743E-5	0.0	0.0	5.157347927369422	0.0
33	7.96772328415743E-5	0.0	0.0	5.364827441688881	0.0
34	7.96772328415743E-5	0.0	0.0	5.599051947165496	0.0
35	7.96772328415743E-5	0.0	0.0	5.808868660314975	0.0
36	7.96772328415743E-5	0.0	0.0	6.05225604756837	0.0
37	7.96772328415743E-5	0.0	0.0	6.366927999137361	0.0
38	7.96772328415743E-5	0.0	0.0	6.697907224361261	0.0
39	7.96772328415743E-5	0.0	0.0	7.104765734327953	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	35295	0.0	43.654907	1
ACTACGG	255	0.0	42.35294	1
CTTACTG	9415	0.0	40.74615	38
TCGGGTA	210	0.0	40.714287	4
CACTTAC	9530	0.0	40.25446	36
CGCACTT	9550	0.0	40.1466	34
ACTTACT	9550	0.0	40.075916	37
GCACTTA	9600	0.0	39.867188	35
TCACGAC	700	0.0	39.857143	24
TAGCATA	2270	0.0	39.845814	29
CCGCACT	9640	0.0	39.725105	33
ATTACGG	340	0.0	39.705883	1
CCCGCAC	9675	0.0	39.697674	32
TAGTAGG	840	0.0	39.642857	1
ACCCGCA	9650	0.0	39.590675	31
CGTTTTA	1830	0.0	39.590164	1
CGGGTAC	695	0.0	39.17266	5
CATATGC	2315	0.0	39.168465	32
GCGCGAC	3690	0.0	39.085365	8
ATCAACG	10300	0.0	38.686893	14
>>END_MODULE