FastQCFastQC Report
Thu 26 May 2016
SRR1043423_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043423_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences865386
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT626327.237463975613194No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65660.7587365637992757No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26100.30159951744077207No Hit
GCGAAGGGTCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCACAAGCCA14340.1657064015364242No Hit
CTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12050.1392442216536898No Hit
CCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC10490.12121758382964364RNA PCR Primer, Index 10 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGCT9140.1056176087896037TruSeq Adapter, Index 10 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTACGG750.045.0000041
GCATAAG406.8121153E-945.08
ACCGGTC207.0323196E-445.040
ACCGGCC207.0323196E-445.020
AAATACG207.0323196E-445.018
CTATCTA207.0323196E-445.038
TAGACTT207.0323196E-445.010
CTATCGG551.8189894E-1245.01
GCAACTA207.0323196E-445.08
GCAACGT207.0323196E-445.016
GCAACGC207.0323196E-445.012
CGTGACA253.8900038E-545.034
TCCTCGA253.8900038E-545.026
GTGATAA207.0323196E-445.014
TTCGTCT207.0323196E-445.036
TATGCGT253.8900038E-545.020
ATCACGG900.045.01
TGTGACG253.8900038E-545.012
ACTAGCG253.8900038E-545.022
TACGCGG800.045.01