##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043423_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	865386
Sequences flagged as poor quality	0
Sequence length	51
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.56669971550268	31.0	31.0	33.0	30.0	34.0
2	30.58530759684118	31.0	31.0	34.0	26.0	34.0
3	31.485578689740763	31.0	31.0	34.0	28.0	34.0
4	35.102291925221806	37.0	35.0	37.0	32.0	37.0
5	35.418198353104856	37.0	35.0	37.0	33.0	37.0
6	35.83495341963008	37.0	35.0	37.0	35.0	37.0
7	33.75468172584257	37.0	35.0	37.0	32.0	37.0
8	34.791677933315306	37.0	35.0	37.0	31.0	37.0
9	36.786263008645854	39.0	37.0	39.0	32.0	39.0
10	36.770082945645065	39.0	35.0	39.0	32.0	39.0
11	36.50891394129325	39.0	35.0	39.0	33.0	39.0
12	36.393701770077165	39.0	35.0	39.0	32.0	39.0
13	36.44578488674418	39.0	35.0	39.0	32.0	39.0
14	37.813335320885706	40.0	36.0	41.0	33.0	41.0
15	37.97087195771598	40.0	36.0	41.0	33.0	41.0
16	37.916426889272536	40.0	36.0	41.0	33.0	41.0
17	37.889902309489635	40.0	36.0	41.0	33.0	41.0
18	37.58111524799338	39.0	36.0	41.0	33.0	41.0
19	37.29403526287691	38.0	35.0	41.0	33.0	41.0
20	36.99086419239507	38.0	35.0	41.0	33.0	41.0
21	36.90498112980797	38.0	35.0	41.0	33.0	41.0
22	36.8174571809574	38.0	35.0	40.0	33.0	41.0
23	36.73371882604988	38.0	35.0	40.0	33.0	41.0
24	36.57486023577918	37.0	35.0	40.0	32.0	41.0
25	36.460458107711474	37.0	35.0	40.0	32.0	41.0
26	36.22293750996665	37.0	35.0	40.0	32.0	41.0
27	36.13802280138574	36.0	35.0	40.0	31.0	41.0
28	36.05898177229583	36.0	35.0	40.0	31.0	41.0
29	35.98742988677885	36.0	35.0	40.0	31.0	41.0
30	35.835261952469764	36.0	35.0	40.0	31.0	41.0
31	35.39777509689318	36.0	35.0	40.0	30.0	41.0
32	35.27391245062897	36.0	35.0	40.0	29.0	41.0
33	35.16040587668393	36.0	35.0	40.0	28.0	41.0
34	34.992371034428565	36.0	35.0	40.0	27.0	41.0
35	34.78484283314035	36.0	35.0	40.0	25.0	41.0
36	34.603948989237175	35.0	34.0	40.0	24.0	41.0
37	34.54290917578977	35.0	34.0	40.0	24.0	41.0
38	34.4448003549861	35.0	34.0	40.0	23.0	41.0
39	34.29642032572748	35.0	34.0	40.0	23.0	41.0
40	34.26831263736645	35.0	34.0	40.0	23.0	41.0
41	34.186008324608906	35.0	34.0	40.0	23.0	41.0
42	34.08312128922816	35.0	34.0	40.0	23.0	41.0
43	33.892855904763884	35.0	34.0	40.0	22.0	41.0
44	33.70761833447733	35.0	33.0	40.0	21.0	41.0
45	33.53216137076403	35.0	33.0	40.0	20.0	41.0
46	33.55006089768034	35.0	33.0	40.0	20.0	41.0
47	33.55896790565135	35.0	33.0	40.0	20.0	41.0
48	33.49929857890005	35.0	33.0	40.0	20.0	41.0
49	33.4128700949634	35.0	33.0	40.0	20.0	41.0
50	33.28159572722461	35.0	33.0	40.0	20.0	41.0
51	32.87702828564363	35.0	33.0	39.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	7.0
13	5.0
14	11.0
15	33.0
16	74.0
17	185.0
18	392.0
19	748.0
20	1289.0
21	2142.0
22	3418.0
23	5477.0
24	8831.0
25	13420.0
26	16503.0
27	18167.0
28	18146.0
29	19405.0
30	21637.0
31	26324.0
32	32655.0
33	43738.0
34	76458.0
35	115914.0
36	103948.0
37	79487.0
38	96820.0
39	160042.0
40	109.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.173737499797777	18.571019175258208	24.980760030783948	31.27448329416006
2	32.35573489749083	20.57914040670868	29.284157589792304	17.780967106008188
3	21.215503832971645	21.700605279031553	39.056213065614656	18.027677822382152
4	21.511325581879067	24.77692035692743	35.87393371281717	17.837820348376333
5	18.93120526562713	31.109008003364973	33.314844474026614	16.644942256981277
6	77.00043679930111	2.1358099160374677	19.210387041158512	1.6533662435028993
7	73.52245125296687	7.338459369576119	17.368549988097797	1.7705393893591992
8	72.74984804468757	3.8938693253646353	20.49859831335381	2.857684316593982
9	37.63060645769634	24.89444016889573	26.76736161666586	10.707591756742078
10	28.5678298470278	23.053065337317683	37.25424261543404	11.12486220022048
11	27.724506751900307	21.26704152828911	37.14712278682576	13.861328932984819
12	17.206541358422715	32.37272153697888	35.63080521293388	14.789931891664528
13	12.420931237621131	34.95908184324683	36.37602179836512	16.24396512076692
14	10.617574122992513	25.89954078295697	47.62730157409526	15.855583519955257
15	12.714441879115215	22.758861363599596	45.64830029605286	18.878396461232327
16	13.074050192630803	24.71070712953526	37.46663338671992	24.748609291114025
17	15.649894960168066	25.254857370005983	41.015685486014334	18.079562183811618
18	18.687614544261173	26.880952546031484	36.52150601003483	17.909926899672516
19	19.593221984178157	26.48956650558248	36.97482972916132	16.94238178107804
20	14.777914133115164	31.051461428772825	36.5768570325843	17.593767405527707
21	16.503849149396917	27.049778942575912	34.8829308539773	21.56344105404987
22	12.18358050627119	30.209409442722666	36.33603964011435	21.27097041089179
23	12.163358316404471	26.218589161368456	46.146921720480805	15.471130801746272
24	13.907435525880937	27.358080671515374	41.66152445267199	17.072959349931708
25	12.44646897453853	32.82939636185471	36.0539689803163	18.670165683290463
26	12.149029450441768	33.567795180416596	36.82426108118227	17.458914287959363
27	12.974557018486548	30.690813116921234	40.60153503754394	15.733094827048276
28	12.166362756041812	26.323166771822056	40.3386465692766	21.171823902859533
29	13.030716928630692	32.3726059816082	38.598960463885476	15.997716625875622
30	19.44392444527644	30.44144462702193	35.11381048456989	15.000820443131735
31	18.342103985966958	29.17622887358936	36.023577917830885	16.4580892226128
32	17.80893150570959	30.716004187726632	34.68302006272346	16.79204424384032
33	14.21169281684705	28.82609610046846	36.61083031156039	20.3513807711241
34	14.913345027536845	29.774459027532224	36.71818125091	18.594014694020935
35	19.693524045917083	29.641570351265216	34.29683401395447	16.368071588863238
36	14.548767833082579	32.941716182143	35.730298387078136	16.779217597696288
37	14.925247230715541	32.39409928055226	35.27304578534897	17.407607703383228
38	14.980020476411681	33.687510544427575	33.44773315029362	17.884735828867118
39	17.439963207169978	29.19298440233607	34.06086994705253	19.306182443441426
40	13.252005463457925	27.333351822192643	37.490668903818644	21.923973810530793
41	15.344597670865948	28.742780678217585	33.107422583679416	22.805199067237048
42	15.982116650835582	28.6339275190493	34.177234205314164	21.206721624800956
43	15.027167067643804	30.54983556470754	35.350005662213164	19.072991705435495
44	12.523544406773393	37.034456300425475	32.27022392319728	18.171775369603854
45	15.73066816426427	32.772889785598565	33.737892686038364	17.758549364098794
46	14.82448294749395	31.402171978747056	33.78249705911582	19.990848014643177
47	14.210190597028378	29.78670789682292	36.840438833075645	19.162662673073054
48	16.018401037225008	28.078221741511882	35.76612055198489	20.137256669278216
49	16.192311870078786	30.785915186980144	34.20958970910091	18.81218323384016
50	13.31567647269542	30.330049249698977	34.737331086936926	21.61694319066867
51	14.179452868430968	28.08862172487191	36.70639460310197	21.025530803595156
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	883.0
1	5898.0
2	10913.0
3	42774.0
4	74635.0
5	42605.0
6	10575.0
7	10565.0
8	10555.0
9	12684.0
10	14813.0
11	17035.5
12	19258.0
13	18963.5
14	18669.0
15	17033.5
16	15398.0
17	13392.5
18	11387.0
19	9834.5
20	8282.0
21	7790.0
22	7298.0
23	6532.0
24	5766.0
25	6309.0
26	7391.5
27	7931.0
28	9298.5
29	10666.0
30	11878.5
31	13091.0
32	14963.5
33	16836.0
34	19994.5
35	23153.0
36	24838.5
37	26524.0
38	30593.0
39	34662.0
40	39630.0
41	44598.0
42	55232.0
43	65866.0
44	70203.0
45	74540.0
46	75032.5
47	75525.0
48	68555.5
49	61586.0
50	52586.5
51	43587.0
52	39141.5
53	34696.0
54	31224.0
55	27752.0
56	25237.5
57	22723.0
58	20968.5
59	19214.0
60	16305.0
61	13396.0
62	11497.5
63	9599.0
64	8196.5
65	6794.0
66	5729.0
67	4664.0
68	4007.5
69	3351.0
70	2992.5
71	2634.0
72	2627.5
73	2621.0
74	2256.0
75	1459.5
76	1028.0
77	832.0
78	636.0
79	479.0
80	322.0
81	225.5
82	129.0
83	94.5
84	60.0
85	36.5
86	13.0
87	8.0
88	3.0
89	3.5
90	4.0
91	3.5
92	3.0
93	2.0
94	1.0
95	2.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	865386.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.15174308709048
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.80262579299853	25.01490035997528
2	9.742212586028208	6.264582323315966
3	3.5974457264071544	3.469924522955832
4	1.9104043240492463	2.456913160771925
5	1.2128127211262727	1.9497021511203514
6	0.8186984738332651	1.5793549797888127
7	0.577303290872905	1.2992914964034257
8	0.42852233552871777	1.1022192031199456
9	0.33001197758370543	0.9549414287040564
>10	2.6202968827801425	18.497860606347462
>50	0.7121671954604397	16.13003823396595
>100	0.24113337141526067	11.355409168002566
>500	0.004118737710509111	0.836201135233987
>1k	0.001497722803821495	0.7581910091712712
>5k	3.7443070095537375E-4	0.7904544563700486
>10k+	3.7443070095537375E-4	7.540015764753106
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	62632	7.237463975613194	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6566	0.7587365637992757	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2610	0.30159951744077207	No Hit
GCGAAGGGTCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCACAAGCCA	1434	0.1657064015364242	No Hit
CTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1205	0.1392442216536898	No Hit
CCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC	1049	0.12121758382964364	RNA PCR Primer, Index 10 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGCT	914	0.1056176087896037	TruSeq Adapter, Index 10 (95% over 24bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.11023982361628222	0.0
2	0.0	0.0	0.0	0.3794838372703048	0.0
3	0.0	0.0	0.0	0.6200701189989207	0.0
4	0.0	0.0	0.0	0.7767632016233218	0.0
5	0.0	0.0	0.0	1.6893039637803247	0.0
6	0.0	0.0	0.0	1.9543879840903366	0.0
7	0.0	0.0	0.0	2.714280101596282	0.0
8	0.0	0.0	0.0	3.604287566473227	0.0
9	0.0	0.0	0.0	4.2741620502296085	0.0
10	0.0	0.0	0.0	4.732107984182781	0.0
11	0.0	0.0	0.0	5.250604932365442	0.0
12	0.0	0.0	0.0	5.685208681443887	0.0
13	0.0	0.0	0.0	5.8667461687616855	0.0
14	0.0	0.0	0.0	6.0117681589487235	0.0
15	0.0	0.0	0.0	6.491785168699286	0.0
16	0.0	0.0	0.0	7.08088644835946	0.0
17	0.0	0.0	0.0	7.819169711550684	0.0
18	0.0	0.0	0.0	8.160751387242225	0.0
19	0.0	0.0	0.0	8.561381857344584	0.0
20	0.0	0.0	0.0	8.973452309142973	0.0
21	0.0	0.0	0.0	9.38170943370935	0.0
22	0.0	0.0	0.0	9.812153189443785	0.0
23	0.0	0.0	0.0	10.148765984196647	0.0
24	0.0010399983360026624	0.0	0.0	10.432223308442707	0.0
25	0.0010399983360026624	0.0	0.0	10.704818427846071	0.0
26	0.0010399983360026624	0.0	0.0	11.00191128583083	0.0
27	0.0010399983360026624	0.0	0.0	11.28767971749023	0.0
28	0.0010399983360026624	0.0	0.0	11.588008125853666	0.0
29	0.0010399983360026624	0.0	0.0	11.95420309549727	0.0
30	0.0010399983360026624	0.0	0.0	12.276833690399428	0.0
31	0.0010399983360026624	0.0	0.0	12.56398878650683	0.0
32	0.0010399983360026624	0.0	0.0	12.866859413024939	0.0
33	0.0010399983360026624	0.0	0.0	13.170192261025715	0.0
34	0.0010399983360026624	0.0	0.0	13.548982766072019	0.0
35	0.0010399983360026624	0.0	0.0	13.864795594104827	0.0
36	0.0010399983360026624	0.0	0.0	14.171710658596279	0.0
37	0.0011555537066696249	0.0	0.0	14.496883471653112	0.0
38	0.0011555537066696249	0.0	0.0	14.819745177296605	0.0
39	0.0011555537066696249	0.0	0.0	15.159824633169476	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTACGG	75	0.0	45.000004	1
GCATAAG	40	6.8121153E-9	45.0	8
ACCGGTC	20	7.0323196E-4	45.0	40
ACCGGCC	20	7.0323196E-4	45.0	20
AAATACG	20	7.0323196E-4	45.0	18
CTATCTA	20	7.0323196E-4	45.0	38
TAGACTT	20	7.0323196E-4	45.0	10
CTATCGG	55	1.8189894E-12	45.0	1
GCAACTA	20	7.0323196E-4	45.0	8
GCAACGT	20	7.0323196E-4	45.0	16
GCAACGC	20	7.0323196E-4	45.0	12
CGTGACA	25	3.8900038E-5	45.0	34
TCCTCGA	25	3.8900038E-5	45.0	26
GTGATAA	20	7.0323196E-4	45.0	14
TTCGTCT	20	7.0323196E-4	45.0	36
TATGCGT	25	3.8900038E-5	45.0	20
ATCACGG	90	0.0	45.0	1
TGTGACG	25	3.8900038E-5	45.0	12
ACTAGCG	25	3.8900038E-5	45.0	22
TACGCGG	80	0.0	45.0	1
>>END_MODULE