##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043421_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2555071
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.532455653874198	31.0	31.0	33.0	30.0	34.0
2	30.55185902857494	31.0	31.0	34.0	26.0	34.0
3	31.49950353630095	31.0	31.0	34.0	28.0	34.0
4	35.12865043671976	37.0	35.0	37.0	32.0	37.0
5	35.462396935349354	37.0	35.0	37.0	33.0	37.0
6	35.78499071062996	37.0	35.0	37.0	35.0	37.0
7	33.73676426212813	37.0	35.0	37.0	32.0	37.0
8	34.74907350911188	37.0	35.0	37.0	31.0	37.0
9	36.790729103026884	39.0	37.0	39.0	32.0	39.0
10	36.80407785145697	39.0	35.0	39.0	32.0	39.0
11	36.65440138454078	39.0	35.0	39.0	33.0	39.0
12	36.65241396423035	39.0	35.0	39.0	33.0	39.0
13	36.662509965476495	39.0	35.0	39.0	33.0	39.0
14	37.82831670822455	40.0	36.0	41.0	33.0	41.0
15	37.93802833659026	40.0	36.0	41.0	33.0	41.0
16	37.82932255111502	40.0	36.0	41.0	33.0	41.0
17	37.71103073065289	40.0	36.0	41.0	32.0	41.0
18	37.48180500659277	39.0	36.0	41.0	32.0	41.0
19	37.23090082428238	38.0	35.0	41.0	32.0	41.0
20	36.830329959519716	38.0	35.0	41.0	32.0	41.0
21	36.78932953330847	38.0	35.0	40.0	32.0	41.0
22	36.741839659250175	38.0	35.0	40.0	32.0	41.0
23	36.66026110429025	38.0	35.0	40.0	32.0	41.0
24	36.552648830502164	38.0	35.0	40.0	32.0	41.0
25	36.442588092463964	38.0	35.0	40.0	31.0	41.0
26	36.152109276024035	37.0	35.0	40.0	31.0	41.0
27	36.00665539235504	37.0	35.0	40.0	31.0	41.0
28	35.85657854517546	37.0	35.0	40.0	30.0	41.0
29	35.73749574865043	37.0	35.0	40.0	30.0	41.0
30	35.55383979544991	37.0	35.0	40.0	30.0	41.0
31	35.222033360325405	37.0	34.0	40.0	27.0	41.0
32	35.02878276180975	37.0	34.0	40.0	25.0	41.0
33	34.60683088649983	37.0	34.0	40.0	24.0	41.0
34	34.46078289018192	37.0	34.0	40.0	23.0	41.0
35	34.28540263656078	36.0	33.0	40.0	22.0	41.0
36	34.16648656730087	36.0	34.0	40.0	21.0	41.0
37	34.03143043774517	36.0	33.0	40.0	21.0	41.0
38	33.907922323880626	36.0	33.0	40.0	21.0	41.0
39	33.77639721166261	36.0	33.0	40.0	20.0	41.0
40	33.66945654347766	35.0	33.0	40.0	20.0	41.0
41	33.54863798305409	35.0	33.0	40.0	18.0	41.0
42	33.43792755661193	35.0	33.0	40.0	18.0	41.0
43	33.259703155019956	35.0	33.0	40.0	18.0	41.0
44	33.038925728482695	35.0	33.0	40.0	18.0	41.0
45	32.80208182081829	35.0	32.0	39.0	18.0	41.0
46	32.791054338607424	35.0	32.0	39.0	18.0	41.0
47	32.754529326190934	35.0	32.0	39.0	18.0	41.0
48	32.662039919830015	35.0	32.0	39.0	18.0	41.0
49	32.57797532827855	35.0	32.0	39.0	18.0	41.0
50	32.49638307506915	35.0	32.0	39.0	18.0	41.0
51	32.07414862444136	35.0	31.0	38.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	3.0
11	12.0
12	16.0
13	31.0
14	70.0
15	154.0
16	316.0
17	745.0
18	1521.0
19	2835.0
20	4885.0
21	7796.0
22	11943.0
23	18826.0
24	29245.0
25	43846.0
26	57379.0
27	66210.0
28	68791.0
29	70902.0
30	75985.0
31	84882.0
32	101216.0
33	131681.0
34	223866.0
35	254316.0
36	274572.0
37	272788.0
38	335326.0
39	414712.0
40	198.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.952295650492687	16.397352558891708	22.56089948185393	33.08945230876167
2	35.20011772666983	18.867655732463014	25.94209710806471	19.990129432802455
3	24.07862638650746	20.55719782346557	36.64841407538186	18.71576171464511
4	22.48505031758413	23.19602860351043	34.0701295580436	20.248791520861847
5	20.761223465023086	31.01929457146201	32.50316723096932	15.716314732545591
6	76.86815747977258	2.360717177722263	19.28748751013181	1.483637832373347
7	72.53199617544874	7.525192059242189	18.186422216838594	1.756389548470473
8	70.8976384609273	3.884745277137113	21.760686885021983	3.4569293769135965
9	41.56287633494333	16.758360139502972	27.386831911911646	14.291931613642047
10	26.895299582673044	18.120357516483885	34.30311721278978	20.68122568805329
11	23.94884525713767	15.554284010111658	41.42174522743204	19.07512550531864
12	21.643742972308793	16.79511058596806	41.723458956717835	19.837687485005308
13	18.55220461584042	19.57836005339969	43.762854339468454	18.106580991291434
14	16.894011947221816	17.926077200985805	45.65004260155588	19.52986825023649
15	21.481868801297498	17.252045050802895	42.47928922523092	18.786796922668685
16	23.018029635967064	16.83726205651428	38.70240787829379	21.442300429224865
17	21.57149449075975	18.659559753916817	40.31829252494353	19.4506532303799
18	23.457626030744354	21.23721806556452	36.73659166418467	18.568564239506454
19	24.835669928546018	20.106838518381682	35.90714308917443	19.150348463897874
20	23.172076235846284	21.96839148501157	38.56358590426645	16.29594637487569
21	22.71404591105296	18.601166073271543	37.93424918524769	20.750538830427804
22	23.298882888185886	20.111378509638282	36.902888412885595	19.68685018929024
23	22.157466465706825	19.41793398304783	38.25948476578537	20.165114785459973
24	24.617202418249825	17.956448176978252	36.71209136654128	20.71425803823064
25	19.879486714850586	20.269378032939205	37.006525454674254	22.84460979753596
26	18.185522046158404	21.350404744134313	38.0601556669071	22.40391754280018
27	19.378756989531798	21.318781356760734	38.01733885281466	21.285122800892815
28	21.961307533137045	21.994300745458737	35.77505282632068	20.269338895083543
29	20.707174086356115	20.558371959135382	38.18144388159859	20.55301007290991
30	24.073655878838593	20.620601149635373	35.042039927657584	20.263703043868446
31	25.56101180749967	20.951942235656073	34.472271024953905	19.014774931890347
32	25.556628367665713	20.709052703427812	33.82665295798042	19.907665970926054
33	21.159842524923963	22.13918908711343	36.65326716948375	20.04770121847886
34	21.25346027566357	24.465582365421547	34.36425054333128	19.9167068155836
35	24.27498100835554	25.304150060800655	32.017936096492036	18.402932834351766
36	23.36189483579908	24.486129739643243	31.79778565840245	20.354189766155226
37	22.025963270687974	27.837308630562518	30.96434502211485	19.17238307663466
38	21.751841729642738	24.05901832082161	32.17926233752409	22.009877612011564
39	21.43670371586543	22.733223460326542	35.36128741627923	20.46878540752879
40	19.75870729228268	23.21285788144439	34.75277203647178	22.275662789801146
41	20.900515093318344	25.51850809625251	30.93878800236862	22.64218880806052
42	19.523762744753473	22.38383982284641	32.67228190527778	25.420115527122338
43	20.288046790089197	21.842054486939894	35.526214340032034	22.343684382938868
44	22.379769485857732	23.40678595624153	33.02976707887961	21.18367747902113
45	22.21930427765021	24.141207817708395	32.85078966494473	20.788698239696664
46	20.339004278159003	25.12936039742144	32.54246163805233	21.989173686367227
47	20.649484887112727	25.285598717217646	33.26424197214089	20.80067442352874
48	22.009016579187033	23.89510898131598	33.38251657194654	20.713357867550453
49	20.37442403753164	23.519229015553776	34.81590139765196	21.290445549262625
50	20.74200677789384	22.985897456469896	35.074093831443435	21.198001934192824
51	20.006019402200565	21.456937987241844	35.22035199804624	23.316690612511355
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1170.0
1	14369.5
2	27569.0
3	108013.5
4	188458.0
5	113082.0
6	37706.0
7	33879.0
8	30052.0
9	29899.0
10	29746.0
11	29984.5
12	30223.0
13	28981.0
14	27739.0
15	26304.5
16	24870.0
17	23926.0
18	22982.0
19	21439.5
20	19897.0
21	18603.0
22	17309.0
23	16607.0
24	15905.0
25	16270.0
26	18496.0
27	20357.0
28	20703.0
29	21049.0
30	23428.5
31	25808.0
32	28315.0
33	30822.0
34	35603.0
35	40384.0
36	44801.0
37	49218.0
38	53079.0
39	56940.0
40	61010.0
41	65080.0
42	73668.0
43	82256.0
44	88569.0
45	94882.0
46	105020.0
47	115158.0
48	128980.5
49	142803.0
50	143702.0
51	144601.0
52	136897.5
53	129194.0
54	127366.5
55	125539.0
56	130082.5
57	134626.0
58	137592.0
59	140558.0
60	142369.5
61	144181.0
62	142260.0
63	140339.0
64	129167.5
65	117996.0
66	100531.5
67	83067.0
68	67568.5
69	52070.0
70	43912.5
71	35755.0
72	32209.0
73	28663.0
74	24804.0
75	16112.0
76	11279.0
77	8476.5
78	5674.0
79	4362.5
80	3051.0
81	2275.0
82	1499.0
83	988.5
84	478.0
85	367.5
86	257.0
87	205.0
88	153.0
89	106.5
90	60.0
91	54.5
92	49.0
93	31.0
94	13.0
95	9.0
96	5.0
97	3.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2555071.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.32728635744668
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.56348554376282	22.74726551423608
2	8.546651012139073	5.013001632603282
3	2.8907565682104486	2.54334136996733
4	1.528565458603629	1.7931470768228173
5	1.0056301051134804	1.4746201031166122
6	0.7294067692012695	1.2834912714855389
7	0.6030774239090002	1.2380637014683358
8	0.4939528954120975	1.1589038408672396
9	0.40506215084293645	1.0691436321300654
>10	5.5693572442035695	37.563678086615305
>50	0.5725103629229248	10.84555880894276
>100	0.08462274018241808	4.361889977207028
>500	0.00437905082420515	0.883195812426393
>1k	0.001836376152086031	1.0047445020299877
>5k	2.8251940801323557E-4	0.4446842652368733
>10k+	4.237791120198533E-4	6.575270404844495
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	131429	5.143849231586911	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	13796	0.5399458566904795	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13492	0.5280479485697267	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5555	0.21741078819336135	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	5179	0.2026949544650618	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	3232	0.12649354949431935	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	3121	0.12214924751601813	No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	2588	0.10128877044904036	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06011574629432998	0.0
2	0.0	0.0	0.0	0.18183447739808403	0.0
3	0.0	0.0	0.0	0.2952559831018394	0.0
4	0.0	0.0	0.0	0.3748623815150342	0.0
5	0.0	0.0	0.0	0.801151905367796	0.0
6	0.0	0.0	0.0	0.9873698225998416	0.0
7	0.0	0.0	0.0	1.4299798322629782	0.0
8	0.0	0.0	0.0	1.9100447697930898	0.0
9	0.0	0.0	0.0	2.3404437684901906	0.0
10	0.0	0.0	0.0	2.6230973620693905	0.0
11	0.0	0.0	0.0	2.9124826668221746	0.0
12	0.0	0.0	0.0	3.1361946497768556	0.0
13	0.0	0.0	0.0	3.253921319603252	0.0
14	0.0	0.0	0.0	3.344016663333426	0.0
15	0.0	0.0	0.0	3.5815834471918784	0.0
16	0.0	0.0	0.0	3.8187979903493874	0.0
17	0.0	0.0	0.0	4.145481671546505	0.0
18	0.0	0.0	0.0	4.328294595336098	0.0
19	0.0	0.0	0.0	4.5451183156945545	0.0
20	0.0	0.0	0.0	4.792626114890741	0.0
21	0.0	0.0	0.0	5.05097510010485	0.0
22	0.0	0.0	0.0	5.28302344631519	0.0
23	0.0	0.0	0.0	5.511118869103833	0.0
24	2.348271339622265E-4	0.0	0.0	5.700546090500029	0.0
25	2.348271339622265E-4	0.0	0.0	5.9040238020782985	0.0
26	2.348271339622265E-4	0.0	0.0	6.107540651512228	0.0
27	2.348271339622265E-4	0.0	0.0	6.311840258059365	0.0
28	2.348271339622265E-4	0.0	0.0	6.518762100935747	0.0
29	2.348271339622265E-4	0.0	0.0	6.738325471190429	0.0
30	2.348271339622265E-4	0.0	0.0	6.96626434255643	0.0
31	2.348271339622265E-4	0.0	0.0	7.192363734706394	0.0
32	2.348271339622265E-4	0.0	0.0	7.444528938726164	0.0
33	2.348271339622265E-4	0.0	0.0	7.6890231230365025	0.0
34	2.348271339622265E-4	0.0	0.0	7.968349998884571	0.0
35	2.348271339622265E-4	0.0	0.0	8.222354682120379	0.0
36	2.348271339622265E-4	0.0	0.0	8.507982752729768	0.0
37	2.348271339622265E-4	0.0	0.0	8.841828661512732	0.0
38	2.348271339622265E-4	0.0	0.0	9.234733594487198	0.0
39	2.348271339622265E-4	0.0	0.0	9.632961275831473	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	35300	0.0	43.98017	1
CGTTTTA	1700	0.0	41.823532	1
ACCCGCA	4335	0.0	41.055363	31
AATGCGG	340	0.0	41.02941	1
CGCACTT	4325	0.0	40.99422	34
TCACGAC	380	0.0	40.855267	24
CCCGCAC	4410	0.0	40.714287	32
CCGCACT	4370	0.0	40.572083	33
CAACGCA	4640	0.0	40.199352	16
TCAACGC	4650	0.0	40.16129	15
ATCAACG	4650	0.0	40.1129	14
CATGCGG	405	0.0	40.0	1
CACTTAC	4445	0.0	39.938133	36
CTTACTG	4400	0.0	39.886364	38
TATGACC	4570	0.0	39.830414	27
AACGCAA	4705	0.0	39.73964	17
CTTATGA	4595	0.0	39.662678	25
CACGACG	375	0.0	39.6	25
GCTTATG	4655	0.0	39.53813	24
CACGACC	575	0.0	39.52174	26
>>END_MODULE