##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043420_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3455155
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.61875747976574	31.0	31.0	33.0	30.0	34.0
2	30.64754750510469	31.0	31.0	34.0	26.0	34.0
3	31.64453722047202	31.0	31.0	34.0	28.0	34.0
4	35.25613293759614	37.0	35.0	37.0	33.0	37.0
5	35.58403776386298	37.0	35.0	37.0	33.0	37.0
6	35.81914993683351	37.0	35.0	37.0	35.0	37.0
7	33.74043306306085	37.0	35.0	37.0	32.0	37.0
8	34.77461503174243	37.0	35.0	37.0	32.0	37.0
9	36.84340847226825	39.0	37.0	39.0	32.0	39.0
10	36.81968565809638	39.0	35.0	39.0	33.0	39.0
11	36.61412092945179	39.0	35.0	39.0	33.0	39.0
12	36.60071950462425	39.0	35.0	39.0	33.0	39.0
13	36.65276782083583	39.0	35.0	39.0	33.0	39.0
14	37.866482690356875	40.0	36.0	41.0	33.0	41.0
15	37.9542558872178	40.0	36.0	41.0	33.0	41.0
16	37.79335919806781	40.0	36.0	41.0	33.0	41.0
17	37.740312663252446	40.0	36.0	41.0	33.0	41.0
18	37.58142572475041	39.0	36.0	41.0	33.0	41.0
19	37.455651338362536	39.0	35.0	41.0	33.0	41.0
20	37.21612749645095	39.0	35.0	41.0	32.0	41.0
21	37.12889870353139	39.0	35.0	41.0	32.0	41.0
22	37.0652459875172	39.0	35.0	41.0	32.0	41.0
23	37.026066558519084	38.0	35.0	41.0	32.0	41.0
24	36.91590247036674	38.0	35.0	41.0	32.0	41.0
25	36.83628664994769	38.0	35.0	40.0	32.0	41.0
26	36.56118784830203	38.0	35.0	40.0	31.0	41.0
27	36.438283666000515	38.0	35.0	40.0	31.0	41.0
28	36.2722746157553	38.0	35.0	40.0	31.0	41.0
29	36.18439375368109	38.0	35.0	40.0	31.0	41.0
30	36.04398181847124	38.0	35.0	40.0	30.0	41.0
31	35.89084889100489	38.0	35.0	40.0	30.0	41.0
32	35.83064522430976	38.0	35.0	40.0	30.0	41.0
33	35.566594262775475	38.0	35.0	40.0	28.0	41.0
34	35.46764993176862	38.0	35.0	40.0	28.0	41.0
35	35.328446046559414	38.0	34.0	40.0	27.0	41.0
36	35.242635424459976	37.0	34.0	40.0	27.0	41.0
37	35.13743696013638	37.0	34.0	40.0	27.0	41.0
38	35.03269723065969	37.0	34.0	40.0	26.0	41.0
39	34.897945244135215	37.0	34.0	40.0	26.0	41.0
40	34.79328828952681	36.0	34.0	40.0	26.0	41.0
41	34.68171500265545	36.0	34.0	40.0	25.0	41.0
42	34.578234550982515	36.0	34.0	40.0	25.0	41.0
43	34.40582781380285	36.0	34.0	40.0	24.0	41.0
44	34.189744309589585	35.0	33.0	40.0	23.0	41.0
45	33.96154268042968	35.0	33.0	40.0	23.0	41.0
46	33.96213744390628	35.0	33.0	40.0	23.0	41.0
47	33.91860162568683	35.0	33.0	39.0	23.0	41.0
48	33.825092072569824	35.0	33.0	39.0	23.0	41.0
49	33.76648370333603	35.0	33.0	39.0	23.0	41.0
50	33.68590584214022	35.0	33.0	39.0	23.0	41.0
51	33.259519182207455	35.0	33.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	1.0
10	1.0
11	9.0
12	13.0
13	30.0
14	60.0
15	129.0
16	328.0
17	641.0
18	1394.0
19	2587.0
20	4535.0
21	7458.0
22	11509.0
23	17618.0
24	26885.0
25	40329.0
26	54538.0
27	64506.0
28	71077.0
29	76945.0
30	87730.0
31	104260.0
32	129342.0
33	177422.0
34	325520.0
35	351291.0
36	352684.0
37	410146.0
38	501998.0
39	633720.0
40	446.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.6526045864802	17.71622401889351	24.39441935311151	27.236752041514777
2	30.30816273076027	21.442887511558816	27.791604139322256	20.457345618358655
3	27.66023521376031	21.861276845756556	31.092121771671604	19.38636616881153
4	24.619474379586446	25.2806892889031	27.50493103782609	22.594905293684363
5	23.013352512405376	34.31122482204127	25.83284975637851	16.84257290917484
6	84.73006276129436	2.1979042908349986	11.733858538907805	1.3381744089628396
7	80.02422467298862	7.478969829139357	11.051660489905663	1.4451450079663575
8	79.71572910621954	3.7205856177219254	13.60338392923038	2.960301346828145
9	46.70215952685191	18.24743028894507	20.064483358923116	14.985926825279908
10	30.647944882356942	18.934259099808838	27.115918099188026	23.3018779186462
11	25.88483005827524	16.130795868781576	36.4523443955481	21.53202967739508
12	22.395666764587986	18.342245138061823	37.61622850494406	21.64585959240613
13	19.26107511819296	22.37505987430376	38.81000997060913	19.55385503689415
14	18.45375967214206	19.092341732860028	40.5499319133295	21.903966681668404
15	23.122175416153546	15.650962113132408	39.13231099617818	22.094551474535876
16	24.142968984025316	16.048860326092463	33.965827871687374	25.84234281819484
17	23.214877480170934	20.0902419717784	34.40699476579198	22.28788578225868
18	25.627214987460768	22.532650488907155	30.565546263481668	21.274588260150413
19	27.31220451759762	20.742108530586904	29.31306989122051	22.632617060594967
20	26.86472821045655	21.916411854171518	32.763074304915406	18.45578563045652
21	24.69165638010451	17.99901885733057	32.24060859787766	25.068716164687256
22	24.7244190202755	20.858195942005498	31.294978083472376	23.122406954246628
23	25.10700677683056	19.982055797786206	33.29679855172923	21.614138873654003
24	27.75536842775505	17.84209391474478	31.550596138234035	22.851941519266138
25	22.347391072180553	20.29234578477666	30.74990847009758	26.610354672945206
26	20.617280556154498	20.334109468316182	33.6413561764957	25.40725379903362
27	21.891376797857117	21.18440417289528	34.207872005742146	22.716347023505456
28	23.602182825372523	22.676667182803666	31.166908575736834	22.554241416086978
29	22.901027594999356	20.525851951649056	34.623106633421656	21.950013819929932
30	28.56317589225375	20.63548523872301	29.876199475855643	20.925139393167598
31	28.72756793834141	21.02611894401264	28.90020852899508	21.34610458865087
32	29.336860430284606	19.4856381262201	27.725500013747574	23.452001429747725
33	23.890447751258627	22.506660337958788	31.336770709273537	22.266121201509048
34	24.704043668084356	22.190900263519293	30.521814506150953	22.583241562245398
35	28.635184239202005	24.41195836366241	27.410174073232607	19.542683323902978
36	24.923686491633514	24.53892806545582	27.75739438606951	22.779991056841155
37	25.578360449820632	28.245129379145073	25.36676357500604	20.80974659602825
38	24.549260452859567	23.72258263377475	26.444486571514158	25.283670341851522
39	24.024132057751387	21.529540642894457	31.526255696198866	22.920071603155286
40	21.97282032209843	22.120454798699335	31.35268895317287	24.554035926029368
41	22.666942582894258	26.40437259688784	26.387528200616178	24.541156619601725
42	21.683600301578366	21.145679426827453	28.71989823900809	28.450822032586093
43	22.75238013924122	20.13041383092799	32.43246685025708	24.684739179573707
44	25.070423758123734	22.83171087838317	29.663039718912753	22.434825644580343
45	25.304913961891724	23.029531236659427	29.36583163418139	22.29972316726746
46	23.72747387599109	24.2350053760251	28.500950029738174	23.536570718245635
47	22.372744493372945	26.233323830624094	29.930813523561174	21.463118152441787
48	24.366982089081386	24.874687242685205	29.63537091678955	21.122959751443858
49	23.05607129057886	22.660488458549615	31.298566923915132	22.984873326956386
50	21.878960567615636	23.51321431310607	32.54496542123292	22.062859698045383
51	21.563460973530855	20.226907331219586	32.35498841585978	25.854643279389784
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1664.0
1	12926.0
2	24188.0
3	80739.0
4	137290.0
5	83533.5
6	29777.0
7	27072.5
8	24368.0
9	24305.5
10	24243.0
11	23993.0
12	23743.0
13	22945.0
14	22147.0
15	20561.0
16	18975.0
17	17825.0
18	16675.0
19	15760.0
20	14845.0
21	14846.0
22	14847.0
23	14535.5
24	14224.0
25	14709.5
26	17343.0
27	19491.0
28	20271.5
29	21052.0
30	25613.0
31	30174.0
32	33906.5
33	37639.0
34	44540.5
35	51442.0
36	55233.5
37	59025.0
38	62577.0
39	66129.0
40	77840.5
41	89552.0
42	107346.5
43	125141.0
44	138562.5
45	151984.0
46	165279.0
47	178574.0
48	200835.5
49	223097.0
50	210790.5
51	198484.0
52	181633.0
53	164782.0
54	170996.5
55	177211.0
56	189678.5
57	202146.0
58	209575.5
59	217005.0
60	216465.5
61	215926.0
62	213075.5
63	210225.0
64	200507.0
65	190789.0
66	164777.5
67	138766.0
68	116104.5
69	93443.0
70	81127.5
71	68812.0
72	63596.5
73	58381.0
74	48862.5
75	30630.0
76	21916.0
77	16334.5
78	10753.0
79	8344.5
80	5936.0
81	4640.5
82	3345.0
83	2416.5
84	1488.0
85	987.0
86	486.0
87	362.5
88	239.0
89	174.0
90	109.0
91	78.0
92	47.0
93	38.0
94	29.0
95	19.5
96	10.0
97	6.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3455155.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.244542016159848
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.46849175361055	17.232511197417224
2	9.475670594737334	4.215639053518499
3	3.1145151375768942	2.0784288851338526
4	1.4771132027269722	1.3143082680273828
5	0.8663975659359009	0.9636308529079883
6	0.5465941908437677	0.7295242466407851
7	0.41522374980079696	0.646552350398589
8	0.3474419348587786	0.6182949374513578
9	0.2599485569437707	0.5204192937278246
>10	3.8517082775898723	21.95570199283044
>50	1.649778101031947	25.92633029557033
>100	0.5171410193031133	16.302800307889083
>500	0.006041751362481465	0.9328957140065451
>1k	0.0029506227584211804	1.2065009498820911
>5k	2.810116912782077E-4	0.34480385677164993
>10k+	7.025292281955193E-4	5.011657797826371
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	92024	2.6633826847131314	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	34013	0.9844131449963895	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	13330	0.38580034759656223	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	10965	0.31735189882943027	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10016	0.289885692537672	No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	5686	0.16456569965746834	No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	5346	0.154725330701517	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	4334	0.12543576192674424	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4090	0.11837385008776741	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.034730713962181144	0.0
2	0.0	0.0	0.0	0.11710039057582076	0.0
3	0.0	0.0	0.0	0.18155480723730194	0.0
4	0.0	0.0	0.0	0.22786242585354347	0.0
5	0.0	0.0	0.0	0.5115834166629283	0.0
6	0.0	0.0	0.0	0.5898432921243765	0.0
7	0.0	0.0	0.0	0.8239283042294774	0.0
8	0.0	0.0	0.0	1.0854505803647014	0.0
9	0.0	0.0	0.0	1.3008099491918597	0.0
10	0.0	0.0	0.0	1.4689934315537219	0.0
11	0.0	0.0	0.0	1.6447887287256289	0.0
12	0.0	0.0	0.0	1.7953463737516842	0.0
13	0.0	0.0	0.0	1.8758348033590388	0.0
14	0.0	0.0	0.0	1.9328800010419214	0.0
15	0.0	0.0	0.0	2.117792110628901	0.0
16	0.0	0.0	0.0	2.3211114986158363	0.0
17	0.0	0.0	0.0	2.588885303264253	0.0
18	0.0	0.0	0.0	2.7412373685116878	0.0
19	0.0	0.0	0.0	2.9126334419150517	0.0
20	0.0	0.0	0.0	3.113840044802621	0.0
21	0.0	0.0	0.0	3.3080426203744837	0.0
22	5.788452327030191E-5	0.0	0.0	3.5155875785601514	0.0
23	5.788452327030191E-5	0.0	0.0	3.7050146809622144	0.0
24	1.4471130817575478E-4	0.0	0.0	3.8641392354322743	0.0
25	1.4471130817575478E-4	0.0	0.0	4.025347632740065	0.0
26	1.4471130817575478E-4	0.0	0.0	4.183111900913273	0.0
27	1.4471130817575478E-4	0.0	0.0	4.3607016183065594	0.0
28	1.4471130817575478E-4	0.0	0.0	4.539275372595441	0.0
29	1.4471130817575478E-4	0.0	0.0	4.732146604132087	0.0
30	1.4471130817575478E-4	0.0	0.0	4.922499858906474	0.0
31	1.4471130817575478E-4	0.0	0.0	5.112997824989038	0.0
32	1.4471130817575478E-4	0.0	0.0	5.341844287738176	0.0
33	1.4471130817575478E-4	0.0	0.0	5.552891259581697	0.0
34	1.4471130817575478E-4	0.0	0.0	5.806049222104363	0.0
35	1.4471130817575478E-4	0.0	0.0	6.0187748451227225	0.0
36	1.4471130817575478E-4	0.0	0.0	6.26762041066175	0.0
37	1.4471130817575478E-4	0.0	0.0	6.538288441473682	0.0
38	1.4471130817575478E-4	0.0	0.0	6.8375803690427785	0.0
39	1.4471130817575478E-4	0.0	0.0	7.196203932963933	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATACGTT	25	3.8924372E-5	45.0	21
CGTTTTT	25610	0.0	43.629444	1
ACGTAGG	265	0.0	43.301888	1
CACGACG	1145	0.0	43.231438	25
CGACGGT	1160	0.0	42.672417	27
CGGGTAC	910	0.0	42.52747	5
ACGGGTA	435	0.0	41.896553	4
CACTTAC	10150	0.0	41.80788	36
GGTACCT	3940	0.0	41.459393	7
ACTTACT	10225	0.0	41.391197	37
CGCACTT	10310	0.0	41.311832	34
ACTACGG	180	0.0	41.25	1
AGGGTAC	1775	0.0	41.19718	5
CTTACTG	10290	0.0	41.086006	38
GCACTTA	10350	0.0	41.04348	35
CATATGC	3865	0.0	41.0414	32
CGGTCTA	1205	0.0	40.892117	30
ACCCGCA	10440	0.0	40.86207	31
CCCGCAC	10505	0.0	40.823418	32
CCGCACT	10430	0.0	40.81496	33
>>END_MODULE