FastQCFastQC Report
Thu 26 May 2016
SRR1043419_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043419_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3520485
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT766212.176433076692558No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC354461.0068499084643168No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC132860.3773911833170714No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC98220.27899564974712293No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83510.23721163419244792No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC66370.18852516059577018No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC55120.156569336327239No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTATCG253.8924518E-545.033
CGTTTTT215150.043.4208681
ACGGGAT5350.042.8971944
GCACTTA105300.041.47435835
CACTTAC105300.041.47435836
ACTTACT105250.041.1947837
CGCACTT106150.041.1422534
ACCCGCA106100.041.01319531
CACGACG7550.040.82781625
CATGCGG7350.040.7142871
ATAGCGG5250.040.7142871
CTTACTG106500.040.6901438
CCGCACT107250.040.63636433
CCCGCAC107850.040.59805332
ACGGGTA3050.040.5737724
ATTACGG2450.040.4081651
ATCAACG112000.040.1785714
TCAACGC112100.040.14272715
TTATGAC110400.040.10869226
CGAATTA451.9303116E-840.016