##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043419_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3520485
Sequences flagged as poor quality	0
Sequence length	51
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.50872649649125	31.0	31.0	33.0	30.0	34.0
2	30.590414104874753	31.0	31.0	34.0	26.0	34.0
3	31.58025215275736	31.0	31.0	34.0	28.0	34.0
4	35.20512145343611	37.0	35.0	37.0	33.0	37.0
5	35.52331937218877	37.0	35.0	37.0	33.0	37.0
6	35.70048047357111	37.0	35.0	37.0	35.0	37.0
7	33.7348101184922	37.0	35.0	37.0	32.0	37.0
8	34.72173834002985	37.0	35.0	37.0	31.0	37.0
9	36.750571015073206	39.0	37.0	39.0	32.0	39.0
10	36.681318057029074	39.0	35.0	39.0	32.0	39.0
11	36.4969215889288	39.0	35.0	39.0	32.0	39.0
12	36.49108347287377	39.0	35.0	39.0	32.0	39.0
13	36.53349325448056	39.0	35.0	39.0	33.0	39.0
14	37.733565403630465	40.0	36.0	41.0	33.0	41.0
15	37.82560811933583	40.0	36.0	41.0	33.0	41.0
16	37.658551875664855	40.0	36.0	41.0	32.0	41.0
17	37.58953496464265	40.0	36.0	41.0	32.0	41.0
18	37.47261953963729	39.0	36.0	41.0	32.0	41.0
19	37.362430460575744	39.0	35.0	41.0	32.0	41.0
20	37.1428013469735	39.0	35.0	41.0	32.0	41.0
21	37.064012770967636	39.0	35.0	41.0	32.0	41.0
22	37.018371900462576	39.0	35.0	41.0	32.0	41.0
23	36.95081217502702	38.0	35.0	41.0	32.0	41.0
24	36.83487417216662	38.0	35.0	41.0	32.0	41.0
25	36.718142528657275	38.0	35.0	40.0	31.0	41.0
26	36.43755732519809	38.0	35.0	40.0	31.0	41.0
27	36.33612215362372	38.0	35.0	40.0	31.0	41.0
28	36.141356659664794	38.0	35.0	40.0	30.0	41.0
29	36.0739219170086	38.0	35.0	40.0	30.0	41.0
30	35.95806060812644	38.0	35.0	40.0	30.0	41.0
31	35.85044106138785	38.0	35.0	40.0	30.0	41.0
32	35.80779977758746	38.0	35.0	40.0	29.0	41.0
33	35.56935706301831	38.0	34.0	40.0	28.0	41.0
34	35.499575200576054	38.0	34.0	40.0	28.0	41.0
35	35.40777676939399	38.0	34.0	40.0	27.0	41.0
36	35.31679271464017	37.0	34.0	40.0	27.0	41.0
37	35.1996222111442	37.0	34.0	40.0	27.0	41.0
38	35.086772703192885	37.0	34.0	40.0	27.0	41.0
39	34.95844578232829	37.0	34.0	40.0	26.0	41.0
40	34.861157482562774	36.0	34.0	40.0	26.0	41.0
41	34.755863183623845	36.0	34.0	40.0	26.0	41.0
42	34.626535264317276	36.0	34.0	40.0	26.0	41.0
43	34.44866687402446	36.0	34.0	40.0	25.0	41.0
44	34.215116667163755	35.0	33.0	40.0	24.0	41.0
45	33.97348490335849	35.0	33.0	40.0	23.0	41.0
46	33.94852754663065	35.0	33.0	40.0	23.0	41.0
47	33.91246973073312	35.0	33.0	39.0	23.0	41.0
48	33.80506492713362	35.0	33.0	39.0	23.0	41.0
49	33.755655825830814	35.0	33.0	39.0	24.0	41.0
50	33.683702387597165	35.0	33.0	39.0	24.0	41.0
51	33.23965277511479	35.0	33.0	38.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	2.0
10	8.0
11	12.0
12	24.0
13	34.0
14	80.0
15	174.0
16	348.0
17	813.0
18	1684.0
19	3106.0
20	5157.0
21	8487.0
22	12700.0
23	18973.0
24	27923.0
25	40278.0
26	53837.0
27	64606.0
28	73118.0
29	80201.0
30	92404.0
31	110151.0
32	136220.0
33	183173.0
34	331489.0
35	351694.0
36	355807.0
37	426627.0
38	520596.0
39	620428.0
40	328.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.222090138148577	18.446833319840874	24.432571080405115	25.89850546160543
2	29.15075621682808	21.78617434813669	27.89570187062294	21.16736756441229
3	27.843947637896484	22.52513503111077	29.64511991955654	19.98579741143621
4	24.76974053290953	25.292083335108657	26.84493755831938	23.093238573662436
5	23.31016891138579	35.166092171959264	24.479468027842756	17.044270888812193
6	86.53793440392445	2.1491357014729506	9.907271299267006	1.405658595335586
7	82.34672211357243	7.113082430403765	9.17424729831259	1.3659481577112245
8	81.69252247914704	3.663330478613032	11.675550385813318	2.9685966564266004
9	47.992819171222145	18.235157939886122	18.191044699806987	15.580978189084743
10	30.22549449862732	17.93352336396832	26.539752335260623	25.301229802143737
11	26.467461159470922	15.294511977753064	36.83651542330105	21.40151143947496
12	22.69448669714542	18.045524977382378	36.775359076945364	22.484629248526836
13	19.83402855004353	21.782027192276065	38.388517491197945	19.99542676648246
14	18.643084688615346	18.956081335384187	39.838658593915326	22.56217538208514
15	23.64713952765031	15.231878562186743	37.90577150591467	23.21521040424828
16	24.255407990660377	16.26824144968662	33.07683458387125	26.399515975781746
17	24.53945976193621	19.750602544819817	33.13049196346526	22.579445729778712
18	26.001389013161543	23.286478993661387	28.701926013035134	22.010205980141944
19	27.878800790232027	20.553986169519256	28.107434060931947	23.45977897931677
20	27.990376325989175	21.885933330208765	30.79095635970612	19.33273398409594
21	25.039987388101352	18.084440070047165	31.237911821808645	25.637660720042838
22	25.957446204145167	20.592247943110113	29.817567749898092	23.632738102846627
23	26.276834015767715	19.783580955464945	31.87060873714843	22.06897629161891
24	28.729109767546234	17.32877714292207	29.15339789830094	24.788715191230754
25	23.227907518424306	20.0250249610494	29.811801498941197	26.935266021585093
26	21.47212670981413	19.955744734035225	32.1976375414183	26.374491014732342
27	22.796262446793552	21.028949136269574	31.98698474784014	24.18780366909673
28	24.762355186856354	22.207678771532898	29.74189635803021	23.288069683580527
29	24.17976500396962	19.675215204723212	33.71853594036049	22.426483850946674
30	29.656453585230448	20.376851484951647	28.669941783589476	21.296753146228433
31	29.985385536367858	20.826590654412673	27.441105415873096	21.74691839334637
32	30.418138409906593	19.381363647338365	26.342535190463813	23.857962752291233
33	25.125004083244214	21.9275753198778	29.599700041329534	23.347720555548452
34	25.211497847597702	23.038359771451944	28.932121568477072	22.81802081247328
35	28.803275685026353	24.644928184610926	25.729239011102162	20.82255711926056
36	26.78312789288976	24.07838692680128	25.25592354462524	23.882561635683718
37	26.443686026215136	27.96577744259669	24.256345361505588	21.334191169682587
38	25.366022011171758	23.746841699368126	24.85975653922684	26.027379750233276
39	24.037455066560433	21.664003681310955	30.333689818306286	23.96485143382233
40	22.863582716585924	22.431767213892403	30.042366321685794	24.662283747835883
41	22.668013072062514	26.32623061879258	25.256065570510884	25.749690738634023
42	22.087610087814603	21.392734239742538	27.208949903209355	29.310705769233504
43	23.083268356490656	19.9018601130242	31.006949326584266	26.007922203900886
44	25.968779869819077	22.116356127067718	28.722690197515398	23.192173805597808
45	26.91555282865855	22.719369632309185	28.459288990011324	21.905788549020944
46	24.21862328628016	23.77885433399091	27.801822760216275	24.20069961951265
47	23.166779577245748	25.619083734201396	28.742857873275984	22.47127881527687
48	25.27648889286561	24.46188522320078	28.656108462328344	21.60551742160526
49	23.367007670818083	22.18077906879308	30.69892358581275	23.753289674576088
50	22.70564993175656	23.3333475359219	31.73713280982592	22.223869722495625
51	22.397198113328134	20.7716266366708	30.742837989651996	26.08833726034907
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1235.0
1	10846.5
2	20458.0
3	68641.0
4	116824.0
5	71270.5
6	25717.0
7	22978.0
8	20239.0
9	20100.0
10	19961.0
11	19802.0
12	19643.0
13	18701.5
14	17760.0
15	16924.0
16	16088.0
17	14944.5
18	13801.0
19	13273.5
20	12746.0
21	12296.0
22	11846.0
23	12346.0
24	12846.0
25	13754.5
26	17277.0
27	19891.0
28	23118.0
29	26345.0
30	28942.0
31	31539.0
32	35977.0
33	40415.0
34	48544.5
35	56674.0
36	60856.0
37	65038.0
38	69146.5
39	73255.0
40	81352.0
41	89449.0
42	99643.0
43	109837.0
44	119179.0
45	128521.0
46	136612.5
47	144704.0
48	165676.5
49	186649.0
50	192534.5
51	198420.0
52	183996.0
53	169572.0
54	182253.5
55	194935.0
56	207495.0
57	220055.0
58	230373.0
59	240691.0
60	246340.5
61	251990.0
62	252497.5
63	253005.0
64	230489.5
65	207974.0
66	180598.5
67	153223.0
68	128798.5
69	104374.0
70	89537.0
71	74700.0
72	70146.5
73	65593.0
74	54452.5
75	33266.5
76	23221.0
77	18040.0
78	12859.0
79	9136.5
80	5414.0
81	4298.5
82	3183.0
83	2177.0
84	1171.0
85	727.0
86	283.0
87	237.0
88	191.0
89	147.0
90	103.0
91	74.0
92	45.0
93	31.5
94	18.0
95	13.0
96	8.0
97	4.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3520485.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.64960397250127
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.98444959287288	16.103480001063126
2	8.824239363319228	3.6443409642219766
3	2.723852338439768	1.6873941620505812
4	1.2824423422076534	1.0592770593661998
5	0.7627255318545187	0.7874990086255609
6	0.5493181084253671	0.6805920836344408
7	0.4150477825813454	0.5999400637978716
8	0.3320100239798288	0.5484700408067292
9	0.2888924977327364	0.5368964101926954
>10	4.40658138877179	22.924549899322308
>50	1.7658399806047955	26.001179723472262
>100	0.6537656202895709	18.551943523875668
>500	0.006382512925828839	0.9073413421901828
>1k	0.0034139022626526352	1.1956075761292657
>5k	5.937221326352409E-4	0.9293789203084392
>10k+	4.452915994764307E-4	3.8421092209426746
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	76621	2.176433076692558	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	35446	1.0068499084643168	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	13286	0.3773911833170714	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	9822	0.27899564974712293	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8351	0.23721163419244792	No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	6637	0.18852516059577018	No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	5512	0.156569336327239	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.02119026213717712	0.0
2	0.0	0.0	0.0	0.07087091693332026	0.0
3	0.0	0.0	0.0	0.11271174284225043	0.0
4	0.0	0.0	0.0	0.13915696274803047	0.0
5	0.0	0.0	0.0	0.3104401808273576	0.0
6	0.0	0.0	0.0	0.35924027513254564	0.0
7	0.0	0.0	0.0	0.5039362474204548	0.0
8	0.0	0.0	0.0	0.6663286450588485	0.0
9	0.0	0.0	0.0	0.7970208650228591	0.0
10	0.0	0.0	0.0	0.9057274778901202	0.0
11	0.0	0.0	0.0	1.0094063744057993	0.0
12	0.0	0.0	0.0	1.0974624235013073	0.0
13	0.0	0.0	0.0	1.1426550603112924	0.0
14	0.0	0.0	0.0	1.178388773137792	0.0
15	0.0	0.0	0.0	1.2849081873662294	0.0
16	0.0	0.0	0.0	1.406880017952072	0.0
17	0.0	0.0	0.0	1.567880561911214	0.0
18	0.0	0.0	0.0	1.6605950600556458	0.0
19	0.0	0.0	0.0	1.758791757385701	0.0
20	0.0	0.0	0.0	1.8799398378348438	0.0
21	0.0	0.0	0.0	2.008473264337158	0.0
22	0.0	0.0	0.0	2.136893070130962	0.0
23	0.0	0.0	0.0	2.2590637369566977	0.0
24	0.0	0.0	0.0	2.356294658264415	0.0
25	0.0	0.0	0.0	2.460456442791263	0.0
26	0.0	0.0	0.0	2.5597041316750393	0.0
27	0.0	0.0	0.0	2.670938805306655	0.0
28	0.0	0.0	0.0	2.786973953872833	0.0
29	0.0	0.0	0.0	2.9071562582996378	0.0
30	0.0	0.0	0.0	3.0275942093205908	0.0
31	0.0	0.0	0.0	3.1574626791479017	0.0
32	0.0	0.0	0.0	3.301249685767728	0.0
33	0.0	0.0	0.0	3.4373956997402346	0.0
34	0.0	0.0	0.0	3.6123715908461476	0.0
35	0.0	0.0	0.0	3.756669890654271	0.0
36	0.0	0.0	0.0	3.9219596163596777	0.0
37	0.0	0.0	0.0	4.117245209111813	0.0
38	0.0	0.0	0.0	4.330539684162836	0.0
39	0.0	0.0	0.0	4.598826582132859	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTATCG	25	3.8924518E-5	45.0	33
CGTTTTT	21515	0.0	43.420868	1
ACGGGAT	535	0.0	42.897194	4
GCACTTA	10530	0.0	41.474358	35
CACTTAC	10530	0.0	41.474358	36
ACTTACT	10525	0.0	41.19478	37
CGCACTT	10615	0.0	41.14225	34
ACCCGCA	10610	0.0	41.013195	31
CACGACG	755	0.0	40.827816	25
CATGCGG	735	0.0	40.714287	1
ATAGCGG	525	0.0	40.714287	1
CTTACTG	10650	0.0	40.69014	38
CCGCACT	10725	0.0	40.636364	33
CCCGCAC	10785	0.0	40.598053	32
ACGGGTA	305	0.0	40.573772	4
ATTACGG	245	0.0	40.408165	1
ATCAACG	11200	0.0	40.17857	14
TCAACGC	11210	0.0	40.142727	15
TTATGAC	11040	0.0	40.108692	26
CGAATTA	45	1.9303116E-8	40.0	16
>>END_MODULE