FastQCFastQC Report
Thu 26 May 2016
SRR1043418_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043418_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1213679
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT774256.379363900998534No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78450.6463817862878075No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31890.2627548140818124No Hit
GTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATT16860.13891646802820184No Hit
CTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14170.11675245266664415No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCACG207.033443E-445.026
TCCGAAT406.8157533E-945.039
CGGTTTA207.033443E-445.041
CATGACG253.8909362E-545.041
CCGTGCT253.8909362E-545.045
ATGCTCG207.033443E-445.019
TCGATTG207.033443E-445.015
CGTTAGA253.8909362E-545.026
TTATACG207.033443E-445.022
TGTGACG207.033443E-445.045
CGCCTCC207.033443E-445.025
GCCGATC351.2120108E-745.08
CCCAACG207.033443E-445.028
TACGCAC253.8909362E-545.014
CCGAGTC351.2120108E-745.011
ATGACGG950.045.01
CGCACGG207.033443E-445.01
CCAACGG750.045.01
ATACGTT207.033443E-445.013
CACGTGC253.8909362E-545.032