##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043418_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1213679
Sequences flagged as poor quality	0
Sequence length	51
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.583061089464348	31.0	31.0	33.0	30.0	34.0
2	30.651523178698817	31.0	31.0	34.0	26.0	34.0
3	31.53732164765148	31.0	31.0	34.0	28.0	34.0
4	35.15548921914279	37.0	35.0	37.0	32.0	37.0
5	35.472166034017235	37.0	35.0	37.0	33.0	37.0
6	35.87607925983724	37.0	35.0	37.0	35.0	37.0
7	33.89072234091552	37.0	35.0	37.0	32.0	37.0
8	34.88220608579369	37.0	35.0	37.0	32.0	37.0
9	36.85862159599037	39.0	37.0	39.0	32.0	39.0
10	36.791821395937475	39.0	35.0	39.0	32.0	39.0
11	36.50849689250617	39.0	35.0	39.0	32.0	39.0
12	36.45574076835803	39.0	35.0	39.0	32.0	39.0
13	36.48946385329234	39.0	35.0	39.0	32.0	39.0
14	37.85284824076218	40.0	36.0	41.0	33.0	41.0
15	38.004168317981936	40.0	36.0	41.0	33.0	41.0
16	37.98060607458809	40.0	36.0	41.0	33.0	41.0
17	37.919631138052154	40.0	36.0	41.0	33.0	41.0
18	37.62598018092098	39.0	36.0	41.0	33.0	41.0
19	37.317621051365315	38.0	35.0	41.0	33.0	41.0
20	37.000009887293096	38.0	35.0	41.0	33.0	41.0
21	36.88708381705541	38.0	35.0	41.0	33.0	41.0
22	36.78729383963964	38.0	35.0	40.0	32.0	41.0
23	36.69457162890682	38.0	35.0	40.0	32.0	41.0
24	36.52311195958734	37.0	35.0	40.0	32.0	41.0
25	36.412464910408765	37.0	35.0	40.0	32.0	41.0
26	36.15421623015641	37.0	35.0	40.0	31.0	41.0
27	36.0569038436028	36.0	35.0	40.0	31.0	41.0
28	35.95562335675248	36.0	35.0	40.0	31.0	41.0
29	35.88880667787776	36.0	35.0	40.0	31.0	41.0
30	35.706154592771234	36.0	35.0	40.0	31.0	41.0
31	35.22864859653994	36.0	34.0	40.0	29.0	41.0
32	35.041284392331086	36.0	34.0	40.0	27.0	41.0
33	34.88477183835265	36.0	34.0	40.0	25.0	41.0
34	34.621128815774185	36.0	34.0	40.0	24.0	41.0
35	34.40819936737803	36.0	34.0	40.0	23.0	41.0
36	34.21272263918219	36.0	34.0	40.0	22.0	41.0
37	34.124250316599365	35.0	34.0	40.0	21.0	41.0
38	34.04251700820398	35.0	34.0	40.0	21.0	41.0
39	33.91951084265279	35.0	33.0	40.0	21.0	41.0
40	33.872225687352255	35.0	33.0	40.0	21.0	41.0
41	33.81516611888316	35.0	33.0	40.0	20.0	41.0
42	33.7330908749348	35.0	33.0	40.0	20.0	41.0
43	33.586097312386556	35.0	33.0	40.0	18.0	41.0
44	33.38468573650858	35.0	33.0	40.0	18.0	41.0
45	33.20302567647624	35.0	33.0	40.0	18.0	41.0
46	33.18620986273965	35.0	33.0	40.0	18.0	41.0
47	33.16803207437881	35.0	33.0	40.0	18.0	41.0
48	33.10684208921799	35.0	33.0	40.0	18.0	41.0
49	33.01764881818009	35.0	33.0	40.0	18.0	41.0
50	32.90031136733848	35.0	33.0	39.0	17.0	41.0
51	32.476939124760335	35.0	32.0	39.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	2.0
11	3.0
12	5.0
13	18.0
14	17.0
15	48.0
16	133.0
17	296.0
18	635.0
19	1177.0
20	2042.0
21	3284.0
22	5413.0
23	8484.0
24	13932.0
25	21240.0
26	26938.0
27	29282.0
28	28576.0
29	28998.0
30	31481.0
31	37067.0
32	45953.0
33	60387.0
34	104570.0
35	148159.0
36	140077.0
37	110425.0
38	145540.0
39	219382.0
40	114.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.05028100510926	18.30871260028393	24.891013192120816	31.749993202485992
2	32.360368763074916	20.441896086197424	29.280971327673953	17.916763823053707
3	20.83681105135707	20.776416169349556	40.56690442860098	17.8198683506924
4	21.78500245946416	24.701012376419136	36.31009517343548	17.203889990681226
5	19.52287219272971	30.420646645447434	34.56861328242476	15.487867879398095
6	76.88383831309596	2.0420556011927373	19.676125235750145	1.3979808499611512
7	73.52290020672682	7.086882116276215	17.865514687161927	1.5247029898350388
8	72.47171616218127	4.030637425546623	20.714043828722424	2.7836025835496865
9	40.14397546633006	22.26000449871836	26.945510303795324	10.650509731156262
10	29.325381752506225	23.870809332615956	36.21361167161993	10.590197243257895
11	27.874256702142823	21.62120297047242	38.068220674494654	12.436319652890097
12	20.085211987683728	29.238126390915554	35.874889488901104	14.801772132499616
13	14.281206150885037	31.58421625487464	36.953922742339614	17.18065485190071
14	12.94131314787518	25.207159388932325	45.0198116635453	16.83171579964719
15	15.28880371168983	22.922123559853965	44.93313306071869	16.855939667737516
16	17.305317139045826	23.97923998025837	36.43302718428843	22.28241569640737
17	17.82843733804408	24.551631856528786	40.8687964445294	16.751134360897733
18	21.324913753966246	25.95521550591219	36.39356040600521	16.32631033411635
19	21.047410394346446	25.938159925317976	36.95128613084679	16.063143549488785
20	17.804543046390357	28.759169434422116	37.360702459216974	16.07558505997055
21	18.31925904625523	26.21129639715279	35.814165030456984	19.655279526135
22	14.995315894894778	29.20467438260034	36.24772283280834	19.552286889696536
23	14.391696651256222	26.284627154296974	44.40778822077337	14.915887973673433
24	15.243239769329453	26.93051457592988	41.22144323169471	16.60480242304596
25	14.591749548274297	30.188954410515468	37.62873049628443	17.5905655449258
26	14.588288995689963	31.575729661632113	36.91882285184139	16.917158490836538
27	14.705206236574911	29.2373848439332	40.45155267578989	15.605856243702002
28	13.635318729252132	26.918567430103018	40.544905201457716	18.901208639187132
29	15.471636239895393	30.41356075206047	38.841571782983806	15.273231225060332
30	19.617213447707343	29.241092578844984	35.84333254509636	15.298361428351317
31	19.914903364069083	27.933415672513078	36.19153005036752	15.96015091305032
32	19.013182233523032	29.489346029716256	35.378629769486004	16.118841967274708
33	17.05706368817455	29.02538480108826	35.06083569049147	18.856715820245714
34	16.735314691940786	28.857630394857285	36.57894715159445	17.828107761607477
35	22.525972683057052	28.34802282975976	33.83233952305346	15.293664964129725
36	16.852726297480636	31.84540558088259	35.11035455009109	16.191513571545688
37	18.014812812943127	30.967084377335357	34.658175679071654	16.35992713064987
38	17.451401894570147	32.29783163422948	33.72176662857312	16.52899984262725
39	19.630561293389768	28.996052498230583	33.81157620754747	17.561810000832182
40	15.439008172671686	28.73197937840236	35.96272160925583	19.86629083967013
41	17.276149624406454	29.752348026125524	32.704364168779385	20.267138180688633
42	17.559338177557656	29.208794088057882	33.386093027892876	19.845774706491586
43	17.55455931922691	29.91499399758915	34.13620899760151	18.394237685582432
44	15.054392471155884	34.64688768611799	32.700986010304206	17.597733832421916
45	18.170784861565537	31.577871908470033	33.74096445600525	16.51037877395918
46	17.283812276557477	31.112098009440718	32.79450332419033	18.809586389811475
47	17.11029028268595	29.94539742386578	35.35778405987086	17.58652823357741
48	17.86897523974626	28.1492882384881	35.476761153484574	18.50497536828107
49	17.794902935619714	30.16143477805911	34.08594859101954	17.957713695301642
50	16.004314155555136	30.905206401363127	33.95189337543123	19.138586067650508
51	15.88978634383556	29.04103968182691	35.76728278235019	19.301891191987337
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1096.0
1	7554.0
2	14012.0
3	55222.0
4	96432.0
5	57207.0
6	17982.0
7	17885.0
8	17788.0
9	20674.5
10	23561.0
11	25849.5
12	28138.0
13	27330.5
14	26523.0
15	23424.5
16	20326.0
17	17592.5
18	14859.0
19	12921.5
20	10984.0
21	10155.0
22	9326.0
23	8726.5
24	8127.0
25	8828.0
26	9833.5
27	10138.0
28	11268.0
29	12398.0
30	15948.5
31	19499.0
32	21055.5
33	22612.0
34	25956.5
35	29301.0
36	33195.5
37	37090.0
38	40914.0
39	44738.0
40	49997.0
41	55256.0
42	65409.0
43	75562.0
44	77297.0
45	79032.0
46	79619.5
47	80207.0
48	82240.0
49	84273.0
50	84018.5
51	83764.0
52	79756.5
53	75749.0
54	65763.5
55	55778.0
56	49997.5
57	44217.0
58	39072.5
59	33928.0
60	28624.5
61	23321.0
62	19550.0
63	15779.0
64	13484.5
65	11190.0
66	8980.5
67	6771.0
68	5347.0
69	3923.0
70	3588.0
71	3253.0
72	3193.5
73	3134.0
74	2576.5
75	1489.5
76	960.0
77	739.0
78	518.0
79	454.5
80	391.0
81	251.5
82	112.0
83	74.5
84	37.0
85	30.5
86	24.0
87	15.0
88	6.0
89	9.0
90	12.0
91	7.0
92	2.0
93	1.0
94	0.0
95	1.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1213679.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.387954652562904
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.25012673133102	26.459996375155438
2	9.01520726913532	6.019986629706913
3	3.2824587797398377	3.28783754660582
4	1.7653363732929912	2.3576388311210508
5	1.1074169768367959	1.8487193902052623
6	0.7305914138948244	1.463577179800333
7	0.5373716577157633	1.2559218377570525
8	0.4106196608290747	1.0967800492169526
9	0.32004840590532463	0.9617185496692823
>10	2.7054161175572644	19.89562990709697
>50	0.6257265952448714	14.800151678857004
>100	0.24460522139382362	11.880505596098027
>500	0.0032986181300025032	0.7162953242547362
>1k	0.0012686992807701934	0.7312905072698856
>5k	2.537398561540387E-4	0.6646170099087411
>10k+	2.537398561540387E-4	6.559333587276517
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	77425	6.379363900998534	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7845	0.6463817862878075	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3189	0.2627548140818124	No Hit
GTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATT	1686	0.13891646802820184	No Hit
CTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1417	0.11675245266664415	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.08264129147822448	0.0
2	0.0	0.0	0.0	0.2825294002779977	0.0
3	0.0	0.0	0.0	0.45712251756848393	0.0
4	0.0	0.0	0.0	0.5657179534292016	0.0
5	0.0	0.0	0.0	1.261371416989171	0.0
6	0.0	0.0	0.0	1.4680158427393075	0.0
7	0.0	0.0	0.0	2.028048602637106	0.0
8	0.0	0.0	0.0	2.6968415866139233	0.0
9	0.0	0.0	0.0	3.1887344182440334	0.0
10	0.0	0.0	0.0	3.5516804690531845	0.0
11	0.0	0.0	0.0	3.9419813641003922	0.0
12	0.0	0.0	0.0	4.249311391232772	0.0
13	0.0	0.0	0.0	4.391276441299553	0.0
14	0.0	0.0	0.0	4.501602153452437	0.0
15	0.0	0.0	0.0	4.865289751243945	0.0
16	0.0	0.0	0.0	5.310135546548964	0.0
17	0.0	0.0	0.0	5.82831209899817	0.0
18	0.0	0.0	0.0	6.1025196942519395	0.0
19	0.0	0.0	0.0	6.418089132299397	0.0
20	0.0	0.0	0.0	6.801633710396241	0.0
21	0.0	0.0	0.0	7.178339577433571	0.0
22	8.239410915077216E-5	0.0	0.0	7.563284855385979	0.0
23	8.239410915077216E-5	0.0	0.0	7.853559301924149	0.0
24	1.6478821830154432E-4	0.0	0.0	8.13856052547667	0.0
25	1.6478821830154432E-4	0.0	0.0	8.415404732223266	0.0
26	1.6478821830154432E-4	0.0	0.0	8.722734759355646	0.0
27	1.6478821830154432E-4	0.0	0.0	8.999990936647993	0.0
28	1.6478821830154432E-4	0.0	0.0	9.297516064791433	0.0
29	1.6478821830154432E-4	0.0	0.0	9.649915669629284	0.0
30	1.6478821830154432E-4	0.0	0.0	9.989297005221315	0.0
31	1.6478821830154432E-4	0.0	0.0	10.29390802675172	0.0
32	1.6478821830154432E-4	0.0	0.0	10.623154886918204	0.0
33	1.6478821830154432E-4	0.0	0.0	10.939218689620567	0.0
34	1.6478821830154432E-4	0.0	0.0	11.321692144298451	0.0
35	1.6478821830154432E-4	0.0	0.0	11.64278198765901	0.0
36	1.6478821830154432E-4	0.0	0.0	11.975983765064733	0.0
37	2.471823274523165E-4	0.0	0.0	12.332420681250973	0.0
38	2.471823274523165E-4	0.0	0.0	12.70657233090463	0.0
39	2.471823274523165E-4	0.0	0.0	13.080641586449135	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCACG	20	7.033443E-4	45.0	26
TCCGAAT	40	6.8157533E-9	45.0	39
CGGTTTA	20	7.033443E-4	45.0	41
CATGACG	25	3.8909362E-5	45.0	41
CCGTGCT	25	3.8909362E-5	45.0	45
ATGCTCG	20	7.033443E-4	45.0	19
TCGATTG	20	7.033443E-4	45.0	15
CGTTAGA	25	3.8909362E-5	45.0	26
TTATACG	20	7.033443E-4	45.0	22
TGTGACG	20	7.033443E-4	45.0	45
CGCCTCC	20	7.033443E-4	45.0	25
GCCGATC	35	1.2120108E-7	45.0	8
CCCAACG	20	7.033443E-4	45.0	28
TACGCAC	25	3.8909362E-5	45.0	14
CCGAGTC	35	1.2120108E-7	45.0	11
ATGACGG	95	0.0	45.0	1
CGCACGG	20	7.033443E-4	45.0	1
CCAACGG	75	0.0	45.0	1
ATACGTT	20	7.033443E-4	45.0	13
CACGTGC	25	3.8909362E-5	45.0	32
>>END_MODULE