##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043417_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	783090
Sequences flagged as poor quality	0
Sequence length	51
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.538996794749007	31.0	31.0	33.0	30.0	34.0
2	30.56882095289175	31.0	31.0	34.0	26.0	34.0
3	31.46290975494515	31.0	31.0	34.0	28.0	34.0
4	35.084797405151384	37.0	35.0	37.0	32.0	37.0
5	35.39767587378207	37.0	35.0	37.0	33.0	37.0
6	35.83574940300604	37.0	35.0	37.0	35.0	37.0
7	33.781823289787894	37.0	35.0	37.0	32.0	37.0
8	34.7952661890715	37.0	35.0	37.0	31.0	37.0
9	36.7690980602485	39.0	37.0	39.0	32.0	39.0
10	36.74873896997791	39.0	35.0	39.0	32.0	39.0
11	36.43557573203591	38.0	35.0	39.0	32.0	39.0
12	36.314745431559594	39.0	35.0	39.0	32.0	39.0
13	36.38580878315392	39.0	35.0	39.0	32.0	39.0
14	37.750884317256	40.0	35.0	41.0	33.0	41.0
15	37.917625049483455	40.0	36.0	41.0	33.0	41.0
16	37.87783907341429	40.0	36.0	41.0	33.0	41.0
17	37.83584134646082	40.0	36.0	41.0	33.0	41.0
18	37.55070681530859	39.0	36.0	41.0	33.0	41.0
19	37.229587914543664	38.0	35.0	41.0	33.0	41.0
20	36.914989337113234	38.0	35.0	41.0	33.0	41.0
21	36.80934247659911	38.0	35.0	41.0	32.0	41.0
22	36.717585462718205	38.0	35.0	40.0	32.0	41.0
23	36.63089810877422	37.0	35.0	40.0	32.0	41.0
24	36.447579460853795	37.0	35.0	40.0	32.0	41.0
25	36.31863004252385	37.0	35.0	40.0	32.0	41.0
26	36.05004150225389	36.0	35.0	40.0	31.0	41.0
27	35.96161360763131	36.0	35.0	40.0	31.0	41.0
28	35.880167030609506	36.0	35.0	40.0	31.0	41.0
29	35.8115389035743	36.0	35.0	40.0	31.0	41.0
30	35.65249588169942	36.0	35.0	40.0	31.0	41.0
31	35.207267363904535	35.0	34.0	40.0	29.0	41.0
32	35.05084856146803	35.0	34.0	40.0	27.0	41.0
33	34.91089529939087	35.0	34.0	40.0	27.0	41.0
34	34.744099656489034	35.0	34.0	40.0	25.0	41.0
35	34.528086171449004	35.0	34.0	40.0	24.0	41.0
36	34.34962520272255	35.0	34.0	40.0	23.0	41.0
37	34.287288817377316	35.0	34.0	40.0	23.0	41.0
38	34.205395293005914	35.0	34.0	40.0	23.0	41.0
39	34.02928399034594	35.0	34.0	40.0	22.0	41.0
40	34.005828193438816	35.0	34.0	40.0	22.0	41.0
41	33.937879426375	35.0	33.0	40.0	22.0	41.0
42	33.86381514257621	35.0	33.0	40.0	21.0	41.0
43	33.678988366598986	35.0	33.0	40.0	21.0	41.0
44	33.495299390874614	35.0	33.0	40.0	20.0	41.0
45	33.315335402061066	35.0	33.0	40.0	20.0	41.0
46	33.342548110689705	35.0	33.0	40.0	20.0	41.0
47	33.315838537077475	35.0	33.0	40.0	20.0	41.0
48	33.26329923763552	35.0	33.0	40.0	20.0	41.0
49	33.16005695386227	35.0	33.0	40.0	20.0	41.0
50	33.0486968292278	35.0	33.0	39.0	18.0	41.0
51	32.60423322989695	35.0	32.0	39.0	17.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	3.0
13	9.0
14	15.0
15	39.0
16	83.0
17	175.0
18	422.0
19	764.0
20	1302.0
21	2124.0
22	3341.0
23	5306.0
24	8630.0
25	12976.0
26	16333.0
27	17543.0
28	17913.0
29	18308.0
30	20282.0
31	24533.0
32	30364.0
33	39986.0
34	69787.0
35	103173.0
36	95844.0
37	68824.0
38	85884.0
39	139043.0
40	81.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.468324202837476	18.796179238657114	25.13133867116168	31.604157887343725
2	32.650270083898405	20.58613952419262	28.605651968483826	18.15793842342515
3	20.97677150774496	21.35182418368259	39.8273506238108	17.844053684761647
4	22.140239308380902	24.667279623031835	36.33707492114572	16.855406147441546
5	18.01338288063952	31.030405189697223	34.827669871917664	16.128542057745598
6	76.65070426132374	1.9276200692129895	19.94036445363879	1.48131121582449
7	73.04907481898633	7.320359090270595	17.843159790062447	1.787406300680637
8	72.28275166328264	3.911044707504884	20.926841103832256	2.8793625253802246
9	38.46020253099899	23.16783511473777	27.03201420015579	11.339948154107446
10	29.37401831207141	23.687187934975544	36.15574199644996	10.783051756503085
11	27.3330013153022	21.70261400349896	37.60908707811363	13.355297603085214
12	17.452272408024623	31.889821093360915	35.92396787087053	14.733938627743937
13	12.770179672834539	34.377657740489596	37.11310321929791	15.739059367377953
14	12.311994789870896	25.060082493710812	47.18257160735037	15.445351109067923
15	13.781046878392011	22.750258590966553	46.32430499687137	17.144389533770067
16	14.438314880792758	24.872236907635138	36.55441903229514	24.13502917927697
17	15.694875429388706	25.436922959046854	41.69367505650691	17.17452655505753
18	19.822114954858318	27.523911683203718	36.34665236435148	16.307320997586483
19	19.816240789692117	26.511639785976072	36.86587748534651	16.806241938985302
20	15.092262702882172	29.766693483507645	38.264184193387734	16.87685962022245
21	16.732048678951333	26.808157427626455	35.3260800163455	21.13371387707671
22	13.24688094599599	30.1559207753898	36.25802908988749	20.33916918872671
23	12.6787470150302	26.84761649363419	45.765876208354086	14.707760282981521
24	14.218416784788465	27.586995109119	41.79953772874127	16.39505037735126
25	13.35810698642557	31.920596610862095	36.43834042064131	18.282955982071027
26	12.995313437791314	33.52999016715831	36.052177910584994	17.42251848446539
27	13.412762262319786	31.374810047376418	40.47491348376304	14.737514206540755
28	13.177157159458044	27.374120471465602	39.53594095187016	19.912781417206197
29	13.436642020712815	32.83492318890549	37.770754319426885	15.957680470954807
30	19.303656029319747	32.1863387350113	34.003626658493914	14.506378577175038
31	19.550626364785657	29.206221507106463	35.866120113907726	15.377032014200157
32	18.49161654471389	31.76084485818999	33.659349500057466	16.088189097038654
33	15.02049572846033	31.79864383404206	34.48735139000625	18.69350904749135
34	16.758482422199236	29.628012105888214	35.51686268500428	18.096642786908273
35	21.346971612458336	30.024645953849493	32.76762568797967	15.860756745712498
36	17.133790496622357	32.880894916293144	34.71899781634295	15.26631677074155
37	17.11667879809473	31.88547931910764	33.95433475079493	17.04350713200271
38	17.11272012156968	32.44135412277005	33.00604017418177	17.4398855814785
39	18.980449245935972	29.450254759989274	33.17038909959264	18.398906894482113
40	14.32900432900433	28.381539797469003	36.02472257339514	21.26473330013153
41	16.01412353624743	29.89273263609547	33.05405508945332	21.03908873820378
42	17.080412213155576	28.739097677150777	33.95625023943608	20.22423987025757
43	16.718895656948753	30.35653628573983	34.15724884751433	18.767319209797087
44	14.805833301408523	35.945293644408686	31.16857577034568	18.08029728383711
45	17.301715000830047	33.2134237443972	32.354390938461734	17.130470316311026
46	16.820161156444343	31.953543015489917	32.553729456384325	18.672566371681416
47	15.31394858828487	30.307116678798096	36.000204318788384	18.378730414128643
48	17.7076708935116	27.51816521728026	35.760257441673375	19.01390644753477
49	17.374503569193838	30.71766974421842	33.136421101022876	18.771405585564878
50	15.369625458121034	31.045856798069188	33.357723888697336	20.22679385511244
51	14.780293452859825	28.771150187079392	35.980921733134124	20.46763462692666
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	811.0
1	5338.0
2	9865.0
3	38467.5
4	67070.0
5	38650.5
6	10231.0
7	10343.0
8	10455.0
9	12884.0
10	15313.0
11	17284.5
12	19256.0
13	18545.5
14	17835.0
15	15796.5
16	13758.0
17	11662.5
18	9567.0
19	8240.5
20	6914.0
21	6478.0
22	6042.0
23	5625.5
24	5209.0
25	5566.0
26	6691.5
27	7460.0
28	8373.0
29	9286.0
30	10168.5
31	11051.0
32	12623.5
33	14196.0
34	16667.5
35	19139.0
36	22146.5
37	25154.0
38	26283.0
39	27412.0
40	30999.0
41	34586.0
42	41958.5
43	49331.0
44	54123.5
45	58916.0
46	60889.5
47	62863.0
48	61656.0
49	60449.0
50	56260.0
51	52071.0
52	45535.0
53	38999.0
54	34111.5
55	29224.0
56	26284.0
57	23344.0
58	21094.0
59	18844.0
60	16077.5
61	13311.0
62	11194.0
63	9077.0
64	7479.5
65	5882.0
66	4910.5
67	3939.0
68	3355.5
69	2772.0
70	2505.0
71	2238.0
72	2257.0
73	2276.0
74	1850.0
75	1111.0
76	798.0
77	571.5
78	345.0
79	304.5
80	264.0
81	168.5
82	73.0
83	77.5
84	82.0
85	51.5
86	21.0
87	13.0
88	5.0
89	5.0
90	5.0
91	3.0
92	1.0
93	2.0
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	783090.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.40271988652725
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.31069846810931	26.157903250485536
2	9.462602601590357	6.321533281968934
3	3.5574788605638523	3.564884096449695
4	1.8526410647637728	2.4753300214632756
5	1.1374064717394954	1.8996234886318817
6	0.7576639102111119	1.5184821216547622
7	0.5793915981732767	1.3547278680872519
8	0.4388307420517215	1.1726512283480437
9	0.3099929751925129	0.9319147665432028
>10	2.7396435635593694	20.495908936602653
>50	0.6663309718483372	15.368914479618665
>100	0.1809689833088479	9.003256632641289
>500	0.004365479860520454	0.9951477176736039
>1k	0.0011905854165055782	0.5763823466690862
>5k	3.9686180550185937E-4	0.7562698453880259
>10k+	3.9686180550185937E-4	7.407069917774116
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	55876	7.135322887535277	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5705	0.7285241798516134	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2253	0.28770639390108416	No Hit
TTTTTGGGATTATTTTACTTCTACTCACAATTCTAGAATTTGCAGTAGCAT	1088	0.1389367761049177	No Hit
CTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1007	0.12859313744269496	No Hit
CCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGC	954	0.12182507757728997	No Hit
CTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGCT	886	0.1131415290707326	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	857	0.10943825103117137	No Hit
GCGAAGGGTCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCACAAGCCA	786	0.10037160479638356	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.11799410029498525	0.0
2	0.0	0.0	0.0	0.38871649491118515	0.0
3	0.0	0.0	0.0	0.6517769349627757	0.0
4	0.0	0.0	0.0	0.8198291384132093	0.0
5	0.0	0.0	0.0	1.787661699166124	0.0
6	0.0	0.0	0.0	2.058511793025067	0.0
7	0.0	0.0	0.0	2.857398255628344	0.0
8	0.0	0.0	0.0	3.7952214943365385	0.0
9	0.0	0.0	0.0	4.485308202122361	0.0
10	0.0	0.0	0.0	4.954858317690176	0.0
11	0.0	0.0	0.0	5.4799576038514095	0.0
12	0.0	0.0	0.0	5.906217676129181	0.0
13	0.0	0.0	0.0	6.087805999310424	0.0
14	0.0	0.0	0.0	6.230573752697646	0.0
15	0.0	0.0	0.0	6.721960438774598	0.0
16	0.0	0.0	0.0	7.2860079939725955	0.0
17	0.0	0.0	0.0	8.012616685183056	0.0
18	0.0	0.0	0.0	8.328289213244965	0.0
19	0.0	0.0	0.0	8.725944655148195	0.0
20	0.0	0.0	0.0	9.137263916024978	0.0
21	0.0	0.0	0.0	9.558543717835754	0.0
22	1.2769924274349054E-4	0.0	0.0	9.987868571939368	0.0
23	2.5539848548698107E-4	0.0	0.0	10.32486687353944	0.0
24	5.107969709739621E-4	0.0	0.0	10.600058741651662	0.0
25	5.107969709739621E-4	0.0	0.0	10.88355106054221	0.0
26	5.107969709739621E-4	0.0	0.0	11.190029243126588	0.0
27	5.107969709739621E-4	0.0	0.0	11.489228568874587	0.0
28	5.107969709739621E-4	0.0	0.0	11.798388435556577	0.0
29	5.107969709739621E-4	0.0	0.0	12.168843938755444	0.0
30	5.107969709739621E-4	0.0	0.0	12.506097638841002	0.0
31	5.107969709739621E-4	0.0	0.0	12.798018107752622	0.0
32	5.107969709739621E-4	0.0	0.0	13.096068140315928	0.0
33	5.107969709739621E-4	0.0	0.0	13.389137902412239	0.0
34	5.107969709739621E-4	0.0	0.0	13.76495677380633	0.0
35	5.107969709739621E-4	0.0	0.0	14.08318328672311	0.0
36	5.107969709739621E-4	0.0	0.0	14.385064296568721	0.0
37	5.107969709739621E-4	0.0	0.0	14.73074614667535	0.0
38	5.107969709739621E-4	0.0	0.0	15.065828959634269	0.0
39	5.107969709739621E-4	0.0	0.0	15.430027199938705	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACATCGA	45	3.8380676E-10	45.000004	12
TTACCGG	45	3.8380676E-10	45.000004	1
CGGGTTC	75	0.0	45.0	5
GTGCAAC	20	7.0319074E-4	45.0	33
AACCGTA	20	7.0319074E-4	45.0	37
ATGGGCG	150	0.0	45.0	4
TGTCAAC	20	7.0319074E-4	45.0	26
CGGCGTA	20	7.0319074E-4	45.0	14
GCGACAA	20	7.0319074E-4	45.0	8
GATCGTC	20	7.0319074E-4	45.0	8
GGTCGTA	25	3.8896633E-5	45.0	7
GGTAACC	20	7.0319074E-4	45.0	7
TTTACGG	20	7.0319074E-4	45.0	1
CGACGGT	150	0.0	45.0	27
TTAGCCG	20	7.0319074E-4	45.0	11
GCAACTC	25	3.8896633E-5	45.0	8
GCAACCG	20	7.0319074E-4	45.0	35
TCGGGTA	20	7.0319074E-4	45.0	4
ACATTGC	20	7.0319074E-4	45.0	36
TAAACCG	20	7.0319074E-4	45.0	19
>>END_MODULE