##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043416_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	297787
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.507436523421102	31.0	31.0	33.0	30.0	34.0
2	30.510196885693464	31.0	31.0	34.0	26.0	34.0
3	31.428329645014724	31.0	31.0	34.0	28.0	34.0
4	35.01771064552852	37.0	35.0	37.0	32.0	37.0
5	35.341099510724106	37.0	35.0	37.0	33.0	37.0
6	35.80478328469678	37.0	35.0	37.0	35.0	37.0
7	33.661818682481105	37.0	35.0	37.0	31.0	37.0
8	34.724994710984696	37.0	35.0	37.0	31.0	37.0
9	36.73669434864517	39.0	37.0	39.0	32.0	39.0
10	36.636572449435334	39.0	35.0	39.0	32.0	39.0
11	36.080550863536686	37.0	35.0	39.0	32.0	39.0
12	35.716988988773856	38.0	35.0	39.0	31.0	39.0
13	35.82901201194142	38.0	35.0	39.0	31.0	39.0
14	37.2397216802614	39.0	35.0	41.0	32.0	41.0
15	37.43222168865666	39.0	35.0	41.0	33.0	41.0
16	37.38413698381729	39.0	35.0	41.0	33.0	41.0
17	37.40234462887903	39.0	35.0	41.0	33.0	41.0
18	37.11435354800579	39.0	35.0	40.0	32.0	41.0
19	36.81492811976346	37.0	35.0	40.0	32.0	41.0
20	36.464409124642785	36.0	35.0	40.0	32.0	41.0
21	36.3770883215184	36.0	35.0	40.0	32.0	41.0
22	36.321820630181975	36.0	35.0	40.0	32.0	41.0
23	36.24810351022711	36.0	35.0	40.0	32.0	41.0
24	36.08560481149278	35.0	35.0	40.0	32.0	41.0
25	35.97930399916719	35.0	35.0	40.0	31.0	41.0
26	35.65490770248533	35.0	35.0	40.0	31.0	41.0
27	35.63952086558513	36.0	35.0	40.0	31.0	41.0
28	35.57927646270657	36.0	35.0	40.0	31.0	41.0
29	35.560578534321515	36.0	35.0	40.0	31.0	41.0
30	35.34312780611645	35.0	35.0	40.0	30.0	41.0
31	34.84193064170027	35.0	34.0	40.0	28.0	41.0
32	34.63133044760181	35.0	34.0	40.0	26.0	41.0
33	34.52759522746124	35.0	34.0	40.0	25.0	41.0
34	34.3166189256079	35.0	34.0	40.0	23.0	41.0
35	34.086222702804356	35.0	34.0	40.0	22.0	41.0
36	33.88550205348118	35.0	33.0	40.0	22.0	41.0
37	33.81911567664136	35.0	33.0	40.0	21.0	41.0
38	33.76958698667168	35.0	33.0	40.0	21.0	41.0
39	33.543156685819056	35.0	33.0	40.0	20.0	41.0
40	33.51976412670802	35.0	33.0	40.0	20.0	41.0
41	33.45275314234671	35.0	33.0	40.0	19.0	41.0
42	33.34676799188682	35.0	33.0	40.0	18.0	41.0
43	33.13049595852069	35.0	33.0	40.0	18.0	41.0
44	32.92112483083546	35.0	33.0	39.0	18.0	41.0
45	32.76794823145403	35.0	33.0	39.0	17.0	41.0
46	32.74801452044582	35.0	33.0	39.0	17.0	41.0
47	32.752494232454744	35.0	33.0	39.0	18.0	41.0
48	32.724628677544686	35.0	33.0	39.0	17.0	41.0
49	32.576509384224295	35.0	33.0	39.0	15.0	41.0
50	32.47936612410884	35.0	32.0	39.0	15.0	41.0
51	31.93709261989274	35.0	31.0	38.0	13.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	1.0
13	4.0
14	13.0
15	24.0
16	52.0
17	101.0
18	204.0
19	414.0
20	628.0
21	1013.0
22	1605.0
23	2405.0
24	3983.0
25	5648.0
26	7219.0
27	7485.0
28	7424.0
29	7561.0
30	8257.0
31	9839.0
32	12072.0
33	16227.0
34	30821.0
35	44932.0
36	27719.0
37	24348.0
38	31784.0
39	45979.0
40	24.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.348991057366504	18.502150866223175	25.12198316246176	31.02687491394856
2	31.183362604814853	21.379711001487642	29.228945521463327	18.20798087223418
3	22.244423027197293	21.46702173029716	38.73540483634276	17.553150406162793
4	22.612471330178955	24.997733279155906	35.07943597269189	17.31035941797325
5	18.437003630111455	32.296574397136204	32.77308949013892	16.49333248261341
6	78.27406837773307	2.524623304576761	17.79090423692102	1.4104040807691403
7	74.16240467179561	7.908337167169822	16.136365925980652	1.7928922350539143
8	74.5623549718423	3.7352201405702736	19.379959501254252	2.3224653863331843
9	44.9811442406821	20.571415139008757	25.183100672628424	9.264339947680725
10	36.991540933620335	20.206389130485885	33.170689116717654	9.631380819176123
11	33.482992877459395	20.600630652110404	34.02028967013335	11.896086800296857
12	17.405729598672878	37.66685583991242	32.51384378767374	12.413570773740963
13	12.380997155685105	41.57132447017499	33.60220560333393	12.44547277080598
14	10.470907057729182	24.10447736133545	53.68803876596358	11.736576814971775
15	11.86418480323184	20.3742943781965	51.81152971754979	15.94999110102187
16	12.771208951364565	19.50555262654179	35.88672440368451	31.83651401840913
17	16.648476931498017	21.33840631055083	44.68630262570226	17.326814132248888
18	21.86529297786673	23.451325947741168	36.663454079593805	18.019926994798297
19	23.49833941710014	22.108083966056274	35.616396954870424	18.777179661973157
20	14.979834579749957	27.91559067387092	39.36034816832165	17.74422657805747
21	17.99877093358676	22.513407234029692	34.99178943338695	24.496032398996597
22	12.162720333661309	28.54892926823535	35.62882194320101	23.65952845490233
23	13.13489171790575	23.108799242411525	48.450402468878764	15.305906570803964
24	13.46969478184071	23.88183500287118	45.63295241229469	17.015517802993415
25	12.20973380302028	31.4436157387663	37.52447218985382	18.8221782683596
26	12.539835520019343	34.669747168278	36.44584887856085	16.34456843314181
27	11.972651593252895	30.243093217635426	43.887745267590596	13.896509921521089
28	12.029067756483661	25.182429051637577	41.51557992793507	21.272923263943692
29	14.170867096280226	30.15175276288085	38.1047527259417	17.572627414897223
30	23.850268816301583	27.447470843253736	33.20729246071857	15.494967879726113
31	23.027868913015006	25.961173590519397	35.0559292380124	15.955028258453192
32	20.272879608579288	29.162790853865346	33.36881730901617	17.195512228539194
33	15.534257707690397	27.787311064620013	35.192268299153426	21.486162928536167
34	15.643060308206872	27.559631548724422	35.91056694885942	20.886741194209286
35	24.596439737127543	27.0750570038316	32.23310621350159	16.09539704553926
36	16.441617666318542	31.480219082767213	34.76410991749136	17.314053333422883
37	17.56490377350254	32.11322186663622	31.222988243274557	19.098886116586687
38	17.756315755892636	30.85964128722879	32.1508326421234	19.233210314755176
39	19.887704970331143	24.615245124871134	31.766665435361517	23.73038446943621
40	13.251417959816916	24.37346156816785	34.111630124887924	28.263490347127306
41	17.93597437094299	25.194182418977324	31.991322656798317	24.878520553281373
42	17.35972356080017	25.455442984415033	33.57231846924144	23.61251498554336
43	16.41441701618942	30.30253167532498	33.81712432040351	19.465926988082085
44	13.243694318422229	37.300150778912446	30.42543831664915	19.03071658601618
45	19.300372413839423	30.312270179692195	32.733128041183804	17.654229365284582
46	18.96792002337241	30.48622001632039	29.757511241256335	20.788348719050866
47	18.34767803832941	27.032744881408522	35.371926914203776	19.24765016605829
48	20.280267439478553	23.099732359035148	34.720454553086604	21.899545648399695
49	18.790947892285427	30.39555118255666	31.85565521664814	18.957845708509772
50	16.593739820744357	30.08962782122793	31.481898135244318	21.8347342227834
51	17.72139146436883	25.92255538354596	36.436781995184475	19.919271156900738
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	514.0
1	1709.0
2	2904.0
3	10639.0
4	18374.0
5	10796.5
6	3219.0
7	3290.5
8	3362.0
9	4069.5
10	4777.0
11	5123.5
12	5470.0
13	5381.0
14	5292.0
15	4860.0
16	4428.0
17	3952.5
18	3477.0
19	2983.5
20	2490.0
21	2515.5
22	2541.0
23	2167.0
24	1793.0
25	1977.0
26	2175.5
27	2190.0
28	2314.5
29	2439.0
30	3277.5
31	4116.0
32	4336.0
33	4556.0
34	5051.0
35	5546.0
36	5852.5
37	6159.0
38	7373.0
39	8587.0
40	12820.0
41	17053.0
42	21948.0
43	26843.0
44	30545.5
45	34248.0
46	32251.0
47	30254.0
48	26934.0
49	23614.0
50	19790.0
51	15966.0
52	14297.0
53	12628.0
54	10946.0
55	9264.0
56	9042.0
57	8820.0
58	8228.0
59	7636.0
60	6319.0
61	5002.0
62	4327.5
63	3653.0
64	3080.0
65	2507.0
66	1997.5
67	1488.0
68	1301.0
69	1114.0
70	1000.5
71	887.0
72	1035.5
73	1184.0
74	985.0
75	493.0
76	200.0
77	147.0
78	94.0
79	81.5
80	69.0
81	52.5
82	36.0
83	33.0
84	30.0
85	20.5
86	11.0
87	6.0
88	1.0
89	1.5
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	1.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	297787.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.348038433182744
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.7145854666597	27.470581538943524
2	9.951374730050551	7.035231528416608
3	3.5242013927494056	3.737208188315464
4	1.9022681358735356	2.6896578870830656
5	1.1791104685932792	2.0839621080401676
6	0.7361402465818859	1.5612668237033476
7	0.5366379604474818	1.327836947542167
8	0.39000940844280674	1.102885404715136
9	0.29026267503314745	0.9234194573511116
>10	3.0183326805595314	25.059607158904434
>50	0.6563327502446618	15.595880393419357
>100	0.09775168620665174	5.214728167175464
>500	9.974661857821607E-4	0.23905244742021395
>1k	9.974661857821607E-4	0.5937526865127438
>5k	0.0	0.0
>10k+	9.974661857821607E-4	5.364929262457191
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15216	5.109692498329343	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1684	0.565504874289341	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	678	0.22767951589558982	No Hit
TAGAAGGGGAATTAAAATCCAACTTATATGTGAAAATTCATTGTTAGGACC	457	0.15346539640749932	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	406	0.1363390611410169	No Hit
GTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATT	377	0.12660055677380141	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	333	0.11182489497526757	No Hit
GAGAAGGGTCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCACAAGCCA	322	0.1081309795256341	No Hit
TAAGCGGGAATCAACAACCGTCTCCATTCTTTCCAACACTGACTAGTTAAA	307	0.10309382209431574	No Hit
CTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	300	0.10074314862636717	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.10175058011263084	0.0
2	0.0	0.0	0.0	0.33849697938459367	0.0
3	0.0	0.0	0.0	0.5265508568204791	0.0
4	0.0	0.0	0.0	0.6833743581821906	0.0
5	0.0	0.0	0.0	1.5010729145328707	0.0
6	0.0	0.0	0.0	1.7018875907947626	0.0
7	0.0	0.0	0.0	2.513541558227861	0.0
8	0.0	0.0	0.0	3.3842981728550945	0.0
9	0.0	0.0	0.0	4.217108201499729	0.0
10	0.0	0.0	0.0	4.62881186888615	0.0
11	0.0	0.0	0.0	5.162414746110475	0.0
12	0.0	0.0	0.0	5.621467693351288	0.0
13	0.0	0.0	0.0	5.787022267593952	0.0
14	0.0	0.0	0.0	5.943174147964821	0.0
15	0.0	0.0	0.0	6.545953987245918	0.0
16	0.0	0.0	0.0	7.2289925349326865	0.0
17	0.0	0.0	0.0	8.34925634765789	0.0
18	0.0	0.0	0.0	8.687081706051641	0.0
19	0.0	0.0	0.0	9.203894058504904	0.0
20	6.716209908424477E-4	0.0	0.0	9.584367349817152	0.0
21	6.716209908424477E-4	0.0	0.0	9.904730562448998	0.0
22	0.0010074314862636717	0.0	0.0	10.348000416405014	0.0
23	0.0010074314862636717	0.0	0.0	10.642506220889429	0.0
24	0.006716209908424478	0.0	0.0	10.897050576418716	0.0
25	0.006716209908424478	0.0	0.0	11.124394281818883	0.0
26	0.006716209908424478	0.0	0.0	11.385319036761175	0.0
27	0.006716209908424478	0.0	0.0	11.635497855849987	0.0
28	0.006716209908424478	0.0	0.0	11.939070543710773	0.0
29	0.006716209908424478	0.0	0.0	12.345065432675034	0.0
30	0.006716209908424478	0.0	0.0	12.704718473271164	0.0
31	0.006716209908424478	0.0	0.0	12.977060785057777	0.0
32	0.006716209908424478	0.0	0.0	13.2796260414323	0.0
33	0.006716209908424478	0.0	0.0	13.579840624338873	0.0
34	0.006716209908424478	0.0	0.0	13.933113265522001	0.0
35	0.006716209908424478	0.0	0.0	14.197060314923082	0.0
36	0.006716209908424478	0.0	0.0	14.511043128141926	0.0
37	0.006716209908424478	0.0	0.0	14.829055667305825	0.0
38	0.006716209908424478	0.0	0.0	15.157814142323204	0.0
39	0.006716209908424478	0.0	0.0	15.49933341616659	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTTCGC	30	2.1600663E-6	45.000004	42
CTTCGCG	30	2.1600663E-6	45.000004	43
CGGGTCG	20	7.024858E-4	45.000004	5
CTGTCAG	20	7.024858E-4	45.000004	16
GAAGCTA	30	2.1600663E-6	45.000004	43
AGTCCGA	20	7.024858E-4	45.000004	23
GCCTTCG	30	2.1600663E-6	45.000004	41
CGAACAG	20	7.024858E-4	45.000004	45
ACGCCCA	20	7.024858E-4	45.000004	26
GATCGTT	20	7.024858E-4	45.000004	35
GATGTAA	20	7.024858E-4	45.000004	40
CGTATAA	30	2.1600663E-6	45.000004	27
GGTAAAC	20	7.024858E-4	45.000004	7
TTTACGG	40	6.7866495E-9	45.000004	1
TGCCACG	20	7.024858E-4	45.000004	22
CCGATGG	40	6.7866495E-9	45.000004	1
TCCGAGA	20	7.024858E-4	45.000004	25
ACACCCG	30	2.1600663E-6	45.000004	28
CACATCG	35	1.2078817E-7	45.000004	16
CGTGACA	20	7.024858E-4	45.000004	20
>>END_MODULE