FastQCFastQC Report
Thu 26 May 2016
SRR1043415_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043415_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences848265
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT471295.55592886656882No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50050.5900278804383065No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20080.23671847830571816No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC9970.11753402533406423No Hit
CTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9200.10845667332732105No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTAC207.032239E-445.00000441
CGGTTGA207.032239E-445.00000425
GAATCGT207.032239E-445.00000420
CGATTCG207.032239E-445.0000049
CACTGCG207.032239E-445.00000415
TATTGCG207.032239E-445.0000041
TATTGCA207.032239E-445.00000433
AGCGGTA207.032239E-445.00000426
CCCGTAG207.032239E-445.00000423
ACCTAGT207.032239E-445.00000443
CGTAGTA207.032239E-445.00000428
AACGCCA650.045.00000414
ACGTGCC207.032239E-445.0000049
AGCGATC207.032239E-445.0000047
TCGAGCG207.032239E-445.0000041
CACGTTA207.032239E-445.00000440
CAATACG207.032239E-445.0000041
ATACCGG207.032239E-445.0000041
ACCCACG207.032239E-445.00000422
CGGACCC207.032239E-445.00000436