##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043415_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	848265
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.54652260791144	31.0	31.0	33.0	30.0	34.0
2	30.590570163804944	31.0	31.0	34.0	26.0	34.0
3	31.50104507435766	31.0	31.0	34.0	28.0	34.0
4	35.10257879318373	37.0	35.0	37.0	32.0	37.0
5	35.41053562271224	37.0	35.0	37.0	33.0	37.0
6	35.83141824783529	37.0	35.0	37.0	35.0	37.0
7	33.778103540756724	37.0	35.0	37.0	32.0	37.0
8	34.79515599488367	37.0	35.0	37.0	32.0	37.0
9	36.74408940602288	39.0	37.0	39.0	32.0	39.0
10	36.709777015437396	39.0	35.0	39.0	32.0	39.0
11	36.49770413726842	39.0	35.0	39.0	32.0	39.0
12	36.384719397829684	39.0	35.0	39.0	32.0	39.0
13	36.43872021125474	39.0	35.0	39.0	32.0	39.0
14	37.78629319846982	40.0	36.0	41.0	33.0	41.0
15	37.944636404897054	40.0	36.0	41.0	33.0	41.0
16	37.87932544664698	40.0	36.0	41.0	33.0	41.0
17	37.85662145673817	40.0	36.0	41.0	33.0	41.0
18	37.61161547393798	39.0	36.0	41.0	33.0	41.0
19	37.333545531172454	38.0	35.0	41.0	33.0	41.0
20	37.03218687556365	38.0	35.0	41.0	33.0	41.0
21	36.9415253488002	38.0	35.0	41.0	32.0	41.0
22	36.85483074275138	38.0	35.0	40.0	32.0	41.0
23	36.77484512504937	38.0	35.0	40.0	32.0	41.0
24	36.66116838487972	38.0	35.0	40.0	32.0	41.0
25	36.59143074393026	38.0	35.0	40.0	32.0	41.0
26	36.31166557620555	37.0	35.0	40.0	31.0	41.0
27	36.24630628400323	37.0	35.0	40.0	31.0	41.0
28	36.15205507712802	37.0	35.0	40.0	31.0	41.0
29	36.11012478411817	37.0	35.0	40.0	31.0	41.0
30	35.95223898192192	37.0	35.0	40.0	31.0	41.0
31	35.57065421772677	37.0	35.0	40.0	30.0	41.0
32	35.44346872734346	37.0	35.0	40.0	29.0	41.0
33	35.3323265724744	37.0	35.0	40.0	28.0	41.0
34	35.17582595061685	37.0	35.0	40.0	27.0	41.0
35	34.96673798871815	37.0	35.0	40.0	25.0	41.0
36	34.80086058012532	37.0	34.0	40.0	24.0	41.0
37	34.704289343542406	37.0	34.0	40.0	24.0	41.0
38	34.624566615385525	37.0	34.0	40.0	23.0	41.0
39	34.48416119962512	36.0	34.0	40.0	23.0	41.0
40	34.43765096992096	36.0	34.0	40.0	23.0	41.0
41	34.36032489846923	36.0	34.0	40.0	23.0	41.0
42	34.26995455429612	36.0	34.0	40.0	22.0	41.0
43	34.10563797869769	36.0	34.0	40.0	21.0	41.0
44	33.9020435830784	35.0	33.0	40.0	21.0	41.0
45	33.724103906208555	35.0	33.0	40.0	20.0	41.0
46	33.723888171738786	35.0	33.0	40.0	20.0	41.0
47	33.73405362710945	35.0	33.0	40.0	20.0	41.0
48	33.68569963395873	35.0	33.0	40.0	20.0	41.0
49	33.58744849781613	35.0	33.0	40.0	20.0	41.0
50	33.48673704561664	35.0	33.0	40.0	20.0	41.0
51	33.047881263520246	35.0	33.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	0.0
11	1.0
12	4.0
13	5.0
14	20.0
15	28.0
16	91.0
17	196.0
18	416.0
19	791.0
20	1386.0
21	2153.0
22	3372.0
23	5446.0
24	8770.0
25	13153.0
26	16661.0
27	18254.0
28	18639.0
29	18741.0
30	21291.0
31	25387.0
32	31418.0
33	41360.0
34	72259.0
35	103159.0
36	88435.0
37	78892.0
38	104187.0
39	173651.0
40	97.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.26018991706601	18.259741943850095	25.002210394157487	30.477857744926407
2	30.74864576517951	21.227328723924717	28.970427873365047	19.053597637530725
3	22.294565967003237	21.255975432205737	38.40651211590718	18.042946484883853
4	22.71106317011783	25.297990604351234	33.65693503798931	18.33401118754163
5	19.42800893588678	31.67913328971489	32.235798954336204	16.657058820062126
6	79.55874638232157	1.8678125349979073	17.1566668434982	1.416774239182331
7	76.05547794616069	7.064419727325777	15.425604027043436	1.4544982994700948
8	75.6726966219283	3.9236559329926384	18.091280437127548	2.312367007951525
9	36.38238050609184	28.39560750473024	24.391434280560908	10.830577708617001
10	27.618845525867506	24.613181022439925	36.87255751445598	10.895415937236596
11	26.41650899188343	21.715973192339657	36.742055843398	15.125461972378915
12	17.099491314624558	30.706559860420978	36.759031670527484	15.434917154426978
13	13.050638656551902	33.47314813177486	37.11410938798607	16.362103823687175
14	12.203969278468403	24.877838882896263	46.83972579323679	16.07846604539855
15	14.785473879035443	23.217980230234655	44.8715908354111	17.124955055318797
16	15.561410644079384	22.789930033656937	36.854402810442494	24.79425651182119
17	16.537167040960078	23.767454745863617	41.37657453743818	18.318803675738124
18	19.655767949874157	25.35610923473207	36.790389795641694	18.197733019752082
19	20.70414316280879	25.24116873854279	36.34571743499968	17.708970663648742
20	16.130218740605827	28.980212551502184	38.00581186303808	16.88375684485391
21	17.939794757534496	24.393202595886898	36.283001184771265	21.384001461807337
22	14.774274548637514	27.237950404649492	37.00353073626756	20.984244310445437
23	13.157444902241636	24.771739963336927	45.146387037069786	16.924428097351655
24	14.110095312196073	26.14348110555074	41.83845850058649	17.907965081666696
25	13.960731610994207	30.156495906350024	36.722250711746916	19.160521770908858
26	13.44579818806623	30.638302888837803	37.08192604905307	18.8339728740429
27	13.673910865118803	28.170206244510855	41.526174014016846	16.629708876353497
28	12.548319216282646	25.088268406688947	40.64567086936276	21.717741507665647
29	14.139095683542289	29.139360930841185	38.719032377853615	18.0025110077629
30	19.777074381236996	28.076131869168243	34.99107000760376	17.155723741991004
31	18.947852381036586	27.279682646342827	36.30787548702351	17.46458948559707
32	17.671718154114576	28.746441265406446	34.75647350768922	18.825367072789753
33	15.348741254207116	28.87045911360247	34.95794356716356	20.822856065026848
34	14.619252238392482	27.662463970575235	36.78296287127254	20.935320919759747
35	20.155729636375426	28.452547258227085	33.69619163822627	17.69553146717123
36	16.272391292815335	30.250452394004235	34.70625335243114	18.770902960749293
37	15.984155894679139	30.841423375949734	34.27631695283903	18.8981037765321
38	16.77848313911337	30.50190683335986	33.416208378278014	19.303401649248762
39	18.716320961020436	27.201994659687717	33.44709495263862	20.63458942665323
40	14.462225837444667	26.968459148968776	35.09327863344592	23.47603638014064
41	15.990993380606296	28.295638744967672	32.63343412730691	23.07993374711912
42	16.36127860986838	26.419691959470214	34.61347574166092	22.60555368900049
43	16.64373751127301	28.483198057210895	34.52258433390509	20.35048009761101
44	14.763193105927982	33.64856501211296	31.676657648258505	19.91158423370055
45	17.812593941751693	30.83175658550099	32.353215091981866	19.002434380765447
46	16.216394640825683	29.857179065504297	32.3002835198906	21.62614277377942
47	15.658314323943578	29.12285665446529	35.4799502513955	19.738878770195633
48	17.427219088374503	26.626349077234117	34.96560626690952	20.98082556748186
49	17.499602129051652	29.333050402881177	32.724089759685945	20.443257708381225
50	16.254826027243848	29.027013963796687	32.88253081289456	21.83562919606491
51	15.579211685027675	26.87179124448138	35.070879972650054	22.478117097840887
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	685.0
1	4770.0
2	8855.0
3	33603.0
4	58351.0
5	33972.5
6	9594.0
7	9296.5
8	8999.0
9	10277.0
10	11555.0
11	12805.5
12	14056.0
13	13867.5
14	13679.0
15	12519.5
16	11360.0
17	10403.0
18	9446.0
19	8443.0
20	7440.0
21	7089.0
22	6738.0
23	6334.0
24	5930.0
25	6697.5
26	8749.0
27	10033.0
28	11159.0
29	12285.0
30	13782.0
31	15279.0
32	16321.5
33	17364.0
34	22544.0
35	27724.0
36	29408.5
37	31093.0
38	33229.5
39	35366.0
40	40669.0
41	45972.0
42	53948.0
43	61924.0
44	64091.5
45	66259.0
46	65126.0
47	63993.0
48	63370.0
49	62747.0
50	57773.5
51	52800.0
52	46653.0
53	40506.0
54	35145.0
55	29784.0
56	27750.5
57	25717.0
58	23242.5
59	20768.0
60	17621.5
61	14475.0
62	12512.5
63	10550.0
64	9191.0
65	7832.0
66	6461.0
67	5090.0
68	4290.5
69	3491.0
70	3612.5
71	3734.0
72	3679.0
73	3624.0
74	3050.5
75	2074.0
76	1671.0
77	1315.5
78	960.0
79	668.0
80	376.0
81	266.5
82	157.0
83	94.5
84	32.0
85	27.0
86	22.0
87	12.5
88	3.0
89	2.0
90	1.0
91	2.0
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	848265.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.38327893591581
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.66831091891497	25.475378559045748
2	9.405588856049718	6.091676149639985
3	3.472069794510741	3.3731101392182765
4	1.7526433776952968	2.270253575003698
5	1.0626480742700208	1.7206014499899929
6	0.7034359109168349	1.366773679005593
7	0.4997258391287894	1.1327932867994521
8	0.37557869644796016	0.9729975751569555
9	0.3075401960351638	0.8963243956991643
>10	2.710032760749512	20.569978095158863
>50	0.8200515722986113	18.59986235715668
>100	0.21744079131210828	10.001187891753805
>500	0.0037947782078901968	0.875695335954262
>1k	3.794778207890196E-4	0.24675782130173424
>5k	3.794778207890196E-4	0.6150512428362449
>10k+	3.794778207890196E-4	5.7915584462795975
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	47129	5.55592886656882	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5005	0.5900278804383065	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2008	0.23671847830571816	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	997	0.11753402533406423	No Hit
CTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	920	0.10845667332732105	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0828750449446812	0.0
2	0.0	0.0	0.0	0.28894272426659123	0.0
3	0.0	0.0	0.0	0.453513937271961	0.0
4	0.0	0.0	0.0	0.5701048610988312	0.0
5	0.0	0.0	0.0	1.2559754322057375	0.0
6	0.0	0.0	0.0	1.4245548266166823	0.0
7	0.0	0.0	0.0	1.9802773897308035	0.0
8	0.0	0.0	0.0	2.60060240608772	0.0
9	0.0	0.0	0.0	3.109759332284133	0.0
10	0.0	0.0	0.0	3.466133814315102	0.0
11	0.0	0.0	0.0	3.90573700435595	0.0
12	0.0	0.0	0.0	4.271070950705263	0.0
13	0.0	0.0	0.0	4.431633982305058	0.0
14	0.0	0.0	0.0	4.562725091805038	0.0
15	0.0	0.0	0.0	5.018714670533383	0.0
16	0.0	0.0	0.0	5.565241993952362	0.0
17	0.0	0.0	0.0	6.281291813289479	0.0
18	0.0	0.0	0.0	6.626938515676115	0.0
19	0.0	0.0	0.0	7.029053420805998	0.0
20	0.0	0.0	0.0	7.447790490000177	0.0
21	0.0	0.0	0.0	7.870182077534733	0.0
22	2.3577537679852405E-4	0.0	0.0	8.312496684408764	0.0
23	2.3577537679852405E-4	0.0	0.0	8.646236730267075	0.0
24	0.0012967645723918823	0.0	0.0	8.950033303271972	0.0
25	0.0012967645723918823	0.0	0.0	9.249585919494498	0.0
26	0.0012967645723918823	0.0	0.0	9.549610086470619	0.0
27	0.0012967645723918823	0.0	0.0	9.845390296664368	0.0
28	0.0012967645723918823	0.0	0.0	10.160268312378797	0.0
29	0.0012967645723918823	0.0	0.0	10.556370945400317	0.0
30	0.0012967645723918823	0.0	0.0	10.911566550547294	0.0
31	0.0027114168331830264	0.0	0.0	11.22526568937773	0.0
32	0.0027114168331830264	0.0	0.0	11.540851031222553	0.0
33	0.0027114168331830264	0.0	0.0	11.869286131102898	0.0
34	0.0027114168331830264	0.0	0.0	12.266213977943213	0.0
35	0.0027114168331830264	0.0	0.0	12.630015384343336	0.0
36	0.0027114168331830264	0.0	0.0	12.982853235722327	0.0
37	0.0027114168331830264	0.0	0.0	13.33073980418855	0.0
38	0.0027114168331830264	0.0	0.0	13.696663188979858	0.0
39	0.0027114168331830264	0.0	0.0	14.095713014211361	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTTAC	20	7.032239E-4	45.000004	41
CGGTTGA	20	7.032239E-4	45.000004	25
GAATCGT	20	7.032239E-4	45.000004	20
CGATTCG	20	7.032239E-4	45.000004	9
CACTGCG	20	7.032239E-4	45.000004	15
TATTGCG	20	7.032239E-4	45.000004	1
TATTGCA	20	7.032239E-4	45.000004	33
AGCGGTA	20	7.032239E-4	45.000004	26
CCCGTAG	20	7.032239E-4	45.000004	23
ACCTAGT	20	7.032239E-4	45.000004	43
CGTAGTA	20	7.032239E-4	45.000004	28
AACGCCA	65	0.0	45.000004	14
ACGTGCC	20	7.032239E-4	45.000004	9
AGCGATC	20	7.032239E-4	45.000004	7
TCGAGCG	20	7.032239E-4	45.000004	1
CACGTTA	20	7.032239E-4	45.000004	40
CAATACG	20	7.032239E-4	45.000004	1
ATACCGG	20	7.032239E-4	45.000004	1
ACCCACG	20	7.032239E-4	45.000004	22
CGGACCC	20	7.032239E-4	45.000004	36
>>END_MODULE