##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043414_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1493742
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.511037381288066	31.0	31.0	33.0	30.0	34.0
2	30.523301212659213	31.0	31.0	34.0	26.0	34.0
3	31.474881873844346	31.0	31.0	34.0	28.0	34.0
4	35.0865551079102	37.0	35.0	37.0	32.0	37.0
5	35.40101235688626	37.0	35.0	37.0	33.0	37.0
6	35.8461615191914	37.0	35.0	37.0	35.0	37.0
7	33.742534520686974	37.0	35.0	37.0	32.0	37.0
8	34.76263839404663	37.0	35.0	37.0	31.0	37.0
9	36.76416543151361	39.0	37.0	39.0	32.0	39.0
10	36.58336446320717	39.0	35.0	39.0	32.0	39.0
11	36.507809916304154	39.0	35.0	39.0	32.0	39.0
12	36.5212044650281	39.0	35.0	39.0	32.0	39.0
13	36.58441953161925	39.0	35.0	39.0	32.0	39.0
14	37.917874706609304	40.0	36.0	41.0	33.0	41.0
15	38.02731864003288	40.0	36.0	41.0	33.0	41.0
16	37.988174664701134	40.0	36.0	41.0	33.0	41.0
17	37.93427646809155	40.0	36.0	41.0	33.0	41.0
18	37.68763481243749	39.0	36.0	41.0	33.0	41.0
19	37.45800211817034	39.0	36.0	41.0	33.0	41.0
20	37.17484277740065	39.0	35.0	41.0	33.0	41.0
21	37.07419018813155	38.0	35.0	41.0	32.0	41.0
22	36.97474128731736	38.0	35.0	41.0	32.0	41.0
23	36.901385245912614	38.0	35.0	40.0	32.0	41.0
24	36.75300420018986	38.0	35.0	40.0	32.0	41.0
25	36.67237916588005	38.0	35.0	40.0	32.0	41.0
26	36.39356127095576	38.0	35.0	40.0	31.0	41.0
27	36.2951681080133	38.0	35.0	40.0	31.0	41.0
28	36.213104404910624	38.0	35.0	40.0	31.0	41.0
29	36.14886238721279	38.0	35.0	40.0	31.0	41.0
30	35.976219454229714	38.0	35.0	40.0	30.0	41.0
31	35.557405495728176	38.0	35.0	40.0	29.0	41.0
32	35.38178480621151	38.0	35.0	40.0	27.0	41.0
33	35.200272871754294	38.0	35.0	40.0	25.0	41.0
34	34.96960787070324	38.0	34.0	40.0	24.0	41.0
35	34.744438463938216	38.0	34.0	40.0	22.0	41.0
36	34.58193315847047	38.0	34.0	40.0	21.0	41.0
37	34.471446876368205	38.0	34.0	40.0	21.0	41.0
38	34.35143619179215	38.0	34.0	40.0	20.0	41.0
39	34.22396973506804	38.0	34.0	40.0	20.0	41.0
40	34.15272717778572	37.0	34.0	40.0	19.0	41.0
41	34.07043117218368	37.0	33.0	40.0	18.0	41.0
42	33.98898738871907	37.0	33.0	40.0	18.0	41.0
43	33.84270242116778	37.0	33.0	40.0	18.0	41.0
44	33.65872285843204	37.0	33.0	40.0	18.0	41.0
45	33.47828272887821	37.0	33.0	40.0	17.0	41.0
46	33.49254021109402	36.0	33.0	40.0	18.0	41.0
47	33.470606704504526	36.0	33.0	40.0	18.0	41.0
48	33.399601135939136	36.0	33.0	40.0	17.0	41.0
49	33.31734195061798	36.0	33.0	40.0	16.0	41.0
50	33.204705364112414	36.0	33.0	40.0	15.0	41.0
51	32.76618318290575	35.0	32.0	40.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	7.0
13	14.0
14	32.0
15	52.0
16	170.0
17	380.0
18	792.0
19	1632.0
20	2730.0
21	4418.0
22	6883.0
23	11192.0
24	17730.0
25	26731.0
26	34334.0
27	37094.0
28	36608.0
29	36481.0
30	38661.0
31	44881.0
32	54523.0
33	70172.0
34	111641.0
35	144411.0
36	141151.0
37	145309.0
38	201864.0
39	323737.0
40	110.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.262031863601614	18.110825028686346	25.704305027240316	29.922838080471724
2	30.901119470430636	20.738052488314583	28.813811220411555	19.547016820843226
3	21.484566946634693	22.55469820089413	36.890306358126104	19.070428494345073
4	22.650564823108677	24.843513806266408	33.552916099299615	18.953005271325303
5	20.666085575688438	31.51735708040612	31.53248686854892	16.284070475356522
6	80.26058047507534	1.878436838490181	16.645377849722376	1.2156048367120962
7	76.53329691472824	7.076523255019944	15.197068837858211	1.1931109923935994
8	75.98500945946489	4.193026640477405	17.719860591721996	2.10210330833571
9	37.81556654361998	30.787579113394415	22.615485137326257	8.781369205659344
10	24.358557234114055	23.570871007175267	40.85190079679088	11.218670961919797
11	23.875274311092546	21.077937153805678	40.515162591665764	14.531625943436016
12	19.73747809193288	25.689443022958447	39.237900520973504	15.33517836413517
13	15.567614755426304	28.973678185389446	39.17637717892381	16.282329880260445
14	15.49156413892091	25.013020990237937	43.775899720299755	15.71951515054139
15	17.906171212967166	24.39725200201909	42.28441056085991	15.412166224153836
16	18.94242780881839	23.276777381903972	37.742059873793465	20.03873493548417
17	18.887599063292054	23.563172221173403	40.80570808077968	16.743520634754862
18	20.080375325859485	25.43417805752265	38.689412227814444	15.79603438880342
19	21.03395365464719	25.190561690037505	37.63715554627238	16.138329109042925
20	19.00843653053874	26.765599414088914	38.62300183030269	15.602962225069659
21	18.435981581826045	24.06854731272201	38.264840916302816	19.23063018914913
22	15.997809528017557	25.957963289510506	39.21433554121126	18.829891641260673
23	16.007181963150263	24.34048182350098	43.33646640450627	16.31586980884249
24	16.753428637609442	25.848774420214465	40.999918325922415	16.397878616253678
25	17.15791615955098	28.484303179531672	37.56980790524736	16.78797275566999
26	16.582448642402774	27.91633361048963	38.29021343712636	17.21100430998124
27	15.890829875574228	26.979090097218933	40.5705938508792	16.55948617632764
28	15.72982482918737	25.182059552452834	40.36814925201273	18.719966366347066
29	16.789713350766064	27.50495065412903	39.093899749755984	16.61143624534893
30	19.45483222671653	26.865482794217478	37.16197308504414	16.517711894021858
31	19.56007128406378	27.222639518738845	36.79892511558221	16.418364081615163
32	19.214964833284462	27.826960746902746	35.881497608020666	17.07657681179213
33	17.239322453275065	27.29132607906854	36.697368086322804	18.77198338133359
34	17.92605416464155	27.571628835501716	36.48468075477559	18.017636245081146
35	20.068927565804536	27.090822913193847	35.338766667871695	17.501482853129925
36	18.225771250992473	29.165946997540402	35.32859088115619	17.279690870310937
37	18.57563086530338	28.960155100412255	34.5893065870813	17.874907447203064
38	18.557622400655536	29.814184778897562	33.989872414379455	17.638320406067447
39	20.09229170767107	28.02632583136847	33.786021950243075	18.095360510717377
40	17.506905476313847	28.814882355855293	34.07087703231214	19.607335135518717
41	18.328399415695614	28.541140304015016	33.37303229071687	19.757427989572495
42	18.13418917055288	27.423209630578775	34.172300169641076	20.27030102922727
43	18.08967010367252	28.688622265424684	34.384853609257824	18.83685402164497
44	17.033195826320743	31.594880508146655	33.07458717770539	18.297336487827216
45	19.0298592394135	29.022347902114287	33.6500546948536	18.297738163618618
46	18.140749875145776	28.642764279239653	33.47773578034226	19.738750065272317
47	17.99909221271143	27.88165560049861	35.05451409948974	19.064738087300217
48	18.735698668176965	27.004596509972938	34.845374904099906	19.414329917750187
49	18.433035959355763	28.587868587748083	33.92306034107631	19.056035111819845
50	17.716446347495083	27.952082755924383	34.283229634033184	20.048241262547347
51	17.925116921128282	26.80790926411656	34.84805274270925	20.418921072045908
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	532.0
1	6440.5
2	12349.0
3	46333.0
4	80317.0
5	50198.5
6	20080.0
7	19456.0
8	18832.0
9	20392.5
10	21953.0
11	22835.0
12	23717.0
13	23037.5
14	22358.0
15	21114.5
16	19871.0
17	18201.5
18	16532.0
19	15515.5
20	14499.0
21	14151.0
22	13803.0
23	14189.0
24	14575.0
25	15386.0
26	18391.0
27	20585.0
28	22682.5
29	24780.0
30	28679.5
31	32579.0
32	34962.0
33	37345.0
34	43606.5
35	49868.0
36	53179.5
37	56491.0
38	62657.0
39	68823.0
40	75328.0
41	81833.0
42	89435.0
43	97037.0
44	100229.5
45	103422.0
46	106090.0
47	108758.0
48	109757.5
49	110757.0
50	102850.5
51	94944.0
52	86224.5
53	77505.0
54	69424.5
55	61344.0
56	55759.5
57	50175.0
58	43964.0
59	37753.0
60	32362.0
61	26971.0
62	22629.5
63	18288.0
64	15210.0
65	12132.0
66	9936.0
67	7740.0
68	6474.5
69	5209.0
70	4584.0
71	3959.0
72	3920.0
73	3881.0
74	3266.5
75	2134.5
76	1617.0
77	1258.5
78	900.0
79	702.0
80	504.0
81	325.0
82	146.0
83	108.5
84	71.0
85	55.0
86	39.0
87	21.5
88	4.0
89	5.0
90	6.0
91	4.0
92	2.0
93	2.5
94	3.0
95	3.0
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1493742.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.614207170287536
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.3006042488898	25.53712128531988
2	9.34480378132651	6.095467329797382
3	3.366995758432656	3.2943569162100608
4	1.6556669931021137	2.1599306527215743
5	1.0727572908256402	1.749356426321191
6	0.7501617117166492	1.4679557686286586
7	0.5297260184041579	1.2093615875407326
8	0.4035321278812713	1.0528704334869396
9	0.3255959526967585	0.9557148469553298
>10	3.2866417921575346	24.680827378498783
>50	0.7757405939933747	17.527693834889654
>100	0.1832464651073311	8.657116713388024
>500	0.0032337610472951473	0.7513437216890549
>1k	8.623362792787059E-4	0.4134991977590615
>5k	2.1558406981967646E-4	0.4355768627983993
>10k+	2.1558406981967646E-4	4.01180704399533
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	57058	3.819802884299966	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6195	0.41473025462228413	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2409	0.1612728302477938	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06547315399848166	0.0
2	0.0	0.0	0.0	0.21201787189487878	0.0
3	0.0	0.0	0.0	0.3407549630391326	0.0
4	0.0	0.0	0.0	0.4271152581905041	0.0
5	0.0	0.0	0.0	0.8903813376071638	0.0
6	0.0	0.0	0.0	1.0203234561256227	0.0
7	0.0	0.0	0.0	1.426016005441368	0.0
8	0.0	0.0	0.0	1.8930980048763442	0.0
9	0.0	0.0	0.0	2.28031346778761	0.0
10	0.0	0.0	0.0	2.5400638128940605	0.0
11	0.0	0.0	0.0	2.852768416500306	0.0
12	0.0	0.0	0.0	3.1223598184960992	0.0
13	0.0	0.0	0.0	3.244469259082224	0.0
14	0.0	0.0	0.0	3.3430806658713488	0.0
15	0.0	0.0	0.0	3.6679024891848795	0.0
16	0.0	0.0	0.0	4.074197552187727	0.0
17	0.0	0.0	0.0	4.562434476636527	0.0
18	0.0	0.0	0.0	4.850235181175866	0.0
19	0.0	0.0	0.0	5.158052729320056	0.0
20	0.0	0.0	0.0	5.517351724728902	0.0
21	0.0	0.0	0.0	5.882876694904475	0.0
22	0.0	0.0	0.0	6.2701591037809745	0.0
23	6.694596523362133E-5	0.0	0.0	6.591499736902357	0.0
24	4.01675791401728E-4	0.0	0.0	6.866045140325438	0.0
25	4.01675791401728E-4	0.0	0.0	7.147887653958984	0.0
26	4.01675791401728E-4	0.0	0.0	7.438232305177199	0.0
27	4.01675791401728E-4	0.0	0.0	7.728242226569247	0.0
28	4.01675791401728E-4	0.0	0.0	8.053331833743712	0.0
29	4.01675791401728E-4	0.0	0.0	8.414505316179099	0.0
30	4.01675791401728E-4	0.0	0.0	8.762289605567762	0.0
31	4.01675791401728E-4	0.0	0.0	9.087513104672695	0.0
32	4.01675791401728E-4	0.0	0.0	9.425657175067716	0.0
33	4.01675791401728E-4	0.0	0.0	9.779667439223106	0.0
34	4.01675791401728E-4	0.0	0.0	10.156573223488394	0.0
35	4.01675791401728E-4	0.0	0.0	10.518215327680416	0.0
36	4.01675791401728E-4	0.0	0.0	10.888091785596174	0.0
37	4.01675791401728E-4	0.0	0.0	11.25903937895567	0.0
38	4.01675791401728E-4	0.0	0.0	11.644715084666561	0.0
39	4.01675791401728E-4	0.0	0.0	12.06339515123763	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCGTTA	20	7.033966E-4	45.0	30
GTCGAAC	25	3.8913695E-5	45.0	33
ACGTTAC	20	7.033966E-4	45.0	34
ATCGTAG	20	7.033966E-4	45.0	10
ATATCCG	20	7.033966E-4	45.0	9
TCGGACC	40	6.8175723E-9	45.0	12
CGTAGCG	35	1.2122473E-7	45.0	27
AGCGCGT	35	1.2122473E-7	45.0	44
TCGTCGA	20	7.033966E-4	45.0	32
CGTCGAC	20	7.033966E-4	45.0	33
CGCCGCA	20	7.033966E-4	45.0	17
CCAGTCG	20	7.033966E-4	45.0	27
CGTCCAG	20	7.033966E-4	45.0	15
TACGTCG	20	7.033966E-4	45.0	1
CGTTTTT	17345	0.0	44.066013	1
CCAGCGG	150	0.0	43.5	1
TTACCGG	120	0.0	43.125004	1
TATCCGG	70	0.0	41.785713	1
GAACGCA	200	0.0	41.625	8
ACGTAGG	120	0.0	41.250004	1
>>END_MODULE