##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043413_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3271626
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.600122691285616	31.0	31.0	33.0	30.0	34.0
2	30.61140912806048	31.0	31.0	34.0	26.0	34.0
3	31.594451505153707	31.0	31.0	34.0	28.0	34.0
4	35.20466734278307	37.0	35.0	37.0	33.0	37.0
5	35.53170716946252	37.0	35.0	37.0	33.0	37.0
6	35.825359011085006	37.0	35.0	37.0	35.0	37.0
7	33.69749170595905	37.0	35.0	37.0	32.0	37.0
8	34.751498796011525	37.0	35.0	37.0	31.0	37.0
9	36.82580160446212	39.0	37.0	39.0	32.0	39.0
10	36.78766704996231	39.0	35.0	39.0	32.0	39.0
11	36.56485643530159	39.0	35.0	39.0	33.0	39.0
12	36.55684940760344	39.0	35.0	39.0	33.0	39.0
13	36.577402796040865	39.0	35.0	39.0	33.0	39.0
14	37.78134236615065	40.0	36.0	41.0	33.0	41.0
15	37.83840726293287	40.0	36.0	41.0	33.0	41.0
16	37.5985192072688	40.0	36.0	41.0	32.0	41.0
17	37.55223579956878	40.0	36.0	41.0	32.0	41.0
18	37.40779600113216	39.0	36.0	41.0	32.0	41.0
19	37.26275864050475	39.0	35.0	41.0	32.0	41.0
20	37.057154760354635	38.0	35.0	41.0	32.0	41.0
21	36.96117435183606	38.0	35.0	41.0	32.0	41.0
22	36.90312584629172	38.0	35.0	40.0	32.0	41.0
23	36.860713296691	38.0	35.0	40.0	32.0	41.0
24	36.747301494730756	38.0	35.0	40.0	32.0	41.0
25	36.63519210325386	38.0	35.0	40.0	32.0	41.0
26	36.39225999548848	38.0	35.0	40.0	31.0	41.0
27	36.258475449210884	38.0	35.0	40.0	31.0	41.0
28	36.10207921076553	37.0	35.0	40.0	31.0	41.0
29	35.93442862967833	37.0	35.0	40.0	30.0	41.0
30	35.7843176451098	37.0	34.0	40.0	30.0	41.0
31	35.61280017948262	37.0	34.0	40.0	29.0	41.0
32	35.54769188165151	37.0	35.0	40.0	29.0	41.0
33	35.24127329957642	37.0	34.0	40.0	26.0	41.0
34	35.12132743779393	37.0	34.0	40.0	25.0	41.0
35	35.02189797978131	37.0	34.0	40.0	25.0	41.0
36	34.947158385463375	37.0	34.0	40.0	25.0	41.0
37	34.840068516389096	37.0	34.0	40.0	24.0	41.0
38	34.72875444809401	37.0	34.0	40.0	24.0	41.0
39	34.6236100947969	37.0	34.0	40.0	23.0	41.0
40	34.52339295506271	36.0	34.0	40.0	23.0	41.0
41	34.43316595478823	36.0	34.0	40.0	23.0	41.0
42	34.29624840981212	36.0	33.0	40.0	23.0	41.0
43	34.12963553902555	35.0	33.0	40.0	23.0	41.0
44	33.918455226850504	35.0	33.0	40.0	22.0	41.0
45	33.686052439979385	35.0	33.0	40.0	21.0	41.0
46	33.69848784671598	35.0	33.0	40.0	22.0	41.0
47	33.66913546964109	35.0	33.0	40.0	22.0	41.0
48	33.591203578893186	35.0	33.0	40.0	22.0	41.0
49	33.55673111779892	35.0	33.0	39.0	22.0	41.0
50	33.48578443868584	35.0	33.0	39.0	22.0	41.0
51	33.0703747922287	35.0	33.0	39.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	3.0
10	6.0
11	6.0
12	11.0
13	40.0
14	57.0
15	162.0
16	294.0
17	708.0
18	1495.0
19	2890.0
20	4765.0
21	7838.0
22	12457.0
23	18937.0
24	28922.0
25	43147.0
26	57663.0
27	68290.0
28	75367.0
29	81142.0
30	90652.0
31	104752.0
32	127940.0
33	170551.0
34	317914.0
35	318865.0
36	322608.0
37	350014.0
38	451275.0
39	612510.0
40	344.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.280455651104376	17.1630864897149	24.162022187132635	29.394435672048086
2	31.663827100041388	20.044528317112043	27.306788734409125	20.984855848437444
3	25.92209500719214	21.4342654080876	33.17420756529017	19.469432019430094
4	23.415604350864065	24.124579031955363	30.408579709294397	22.05123690788617
5	21.531128558093133	33.365855388115875	28.506070070356454	16.596945983434537
6	81.83670749651702	2.1233784057224145	14.67417119193942	1.3657429058211423
7	77.28881601992403	7.532982070689009	13.665712401111863	1.51248950827509
8	76.42655364641313	3.82519273291018	16.582732867387655	3.1655207532890377
9	43.579828501179534	20.474773094479627	22.439637048978092	13.505761355362747
10	30.101545836840764	18.872786803870614	31.469000429755724	19.5566669295329
11	27.678683321382092	15.834175422251809	37.985821117695	18.501320138671108
12	22.68440830339409	17.817287183804016	38.784109186074446	20.714195326727445
13	18.82259769301259	21.0100726672303	41.142661172151094	19.024668467606016
14	19.981165328799808	19.270478960614692	41.3615431592731	19.386812551312403
15	25.060596779705257	15.847074207137368	39.716000545294605	19.37632846786277
16	23.692072382356663	16.649396966523682	37.27354532577991	22.384985325339755
17	24.099637305731157	19.77298138601417	36.692886045043046	19.434495263211627
18	24.27190638538757	24.177946990273337	32.922986918431384	18.627159705907705
19	25.304053702959934	22.76323760723261	31.959796138067126	19.972912551740325
20	25.70440508786762	23.861193180394093	34.16411289065437	16.270288841083914
21	22.097055103486767	19.25275077285729	36.47449311137642	22.17570101227952
22	23.458182567322794	20.966455212178897	35.39285969728814	20.18250252321017
23	25.18854538996817	19.834051936254326	36.0984110041918	18.878991669585705
24	26.087731299360012	18.07342893105752	33.14602585992409	22.692813909658376
25	20.259131086499497	20.083377500973523	35.26133488363279	24.396156528894196
26	18.709840305707313	20.28168867712874	36.56191141652499	24.44655960063895
27	19.53377311465308	23.039033190224064	36.51053023786949	20.916663457253364
28	23.13125033240352	23.42361260119586	33.497685860180844	19.94745120621978
29	21.963451812646067	20.738006116836093	38.19006206699665	19.108480003521187
30	27.882251822182607	20.72354847406152	33.41164301787551	17.982556685880354
31	27.757757151948297	21.0866400988377	32.1203279347945	19.0352748144195
32	27.665876234019414	19.645216170797028	31.66480520695214	21.024102388231416
33	23.108723307615232	22.104910524613754	34.46741773051076	20.318948437260246
34	21.937837637920715	24.397440294214558	33.0711701154105	20.593551952454224
35	24.303542030782246	27.686294215781388	30.002970999741414	18.00719275369495
36	24.42012014820765	25.606197040859808	29.326762900160347	20.646919910772198
37	23.46667987110996	29.242584574153646	28.111556761072322	19.179178793664068
38	24.03499055209856	24.84926455530064	28.056843905752064	23.05890098684874
39	23.13687444714035	22.071135270351807	32.070964101642424	22.721026180865415
40	20.458634330452195	23.914622270393988	32.609901009467464	23.01684238968635
41	20.421802492094145	26.36945665549791	28.439681063789074	24.76905978861887
42	19.640111675356536	21.270218539649704	31.026804408572374	28.062865376421385
43	20.602782836424456	20.642487863832844	33.37435269190305	25.38037660783965
44	23.989569712430455	22.48548581041965	31.383018719132323	22.14192575801757
45	24.21649051572521	24.02386458598874	31.172939694207102	20.58670520407895
46	21.635633168338924	23.97981920916388	30.84927800427066	23.535269618226533
47	19.74269063762178	25.56551390654066	33.57422272594729	21.117572729890274
48	22.889535662083624	24.337653509294764	32.526120039393255	20.246690789228353
49	21.542407353407754	21.87178485560391	33.49664662158817	23.089161169400167
50	19.738900473342614	22.941069669943936	35.90315641213268	21.416873444580766
51	19.373822068903966	20.851405386801545	34.18214673682138	25.5926258074731
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1664.0
1	15458.0
2	29252.0
3	107575.0
4	185898.0
5	111433.5
6	36969.0
7	32234.0
8	27499.0
9	27457.0
10	27415.0
11	27622.0
12	27829.0
13	26621.5
14	25414.0
15	23816.5
16	22219.0
17	20562.5
18	18906.0
19	17665.5
20	16425.0
21	15533.5
22	14642.0
23	15394.5
24	16147.0
25	18221.5
26	21249.5
27	22203.0
28	25355.0
29	28507.0
30	31095.5
31	33684.0
32	38718.0
33	43752.0
34	51245.5
35	58739.0
36	63000.0
37	67261.0
38	70925.0
39	74589.0
40	84159.0
41	93729.0
42	106817.0
43	119905.0
44	134587.5
45	149270.0
46	163635.0
47	178000.0
48	194588.0
49	211176.0
50	200310.0
51	189444.0
52	174086.5
53	158729.0
54	159243.5
55	159758.0
56	166295.5
57	172833.0
58	178831.5
59	184830.0
60	189109.5
61	193389.0
62	192874.0
63	192359.0
64	177072.0
65	161785.0
66	133550.0
67	105315.0
68	83907.5
69	62500.0
70	53625.5
71	44751.0
72	41540.0
73	38329.0
74	32653.5
75	20382.0
76	13786.0
77	10643.0
78	7500.0
79	5792.5
80	4085.0
81	2996.5
82	1908.0
83	1536.5
84	1165.0
85	766.5
86	368.0
87	312.5
88	257.0
89	181.5
90	106.0
91	75.0
92	44.0
93	27.0
94	10.0
95	8.0
96	6.0
97	3.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3271626.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.102408212242988
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.44990291871409	18.426267679331936
2	9.482965087458798	4.571245912007609
3	3.1740935627728306	2.2950989626141043
4	1.5801152166291366	1.5233832789428885
5	0.9214674533377109	1.1104792357320739
6	0.6952246468134021	1.0053952942025446
7	0.5193612318106942	0.8762499493100295
8	0.4137389893711952	0.7977684812116328
9	0.3460201955505585	0.7505928002555735
>10	4.818324789428293	28.41054700216515
>50	1.3071042200133416	21.58685081026202
>100	0.2806287832855989	9.865720409094266
>500	0.006822780749646592	1.1140850127815651
>1k	0.0030020235298445007	1.093233330131051
>5k	6.822780749646592E-4	1.0267644805463683
>10k+	5.458224599717273E-4	5.546317361411254
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	120179	3.673372200856699	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	24382	0.7452563343120515	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12799	0.39121219846033745	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	11277	0.3446909885176362	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	9299	0.2842317550967012	No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	6131	0.18739917093212977	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	5425	0.16581968721363627	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5312	0.16236574718503888	No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	5052	0.15441862853516874	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	3442	0.10520762458789605	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03912427643012985	0.0
2	0.0	0.0	0.0	0.12788747858098695	0.0
3	0.0	0.0	0.0	0.20631942648701287	0.0
4	0.0	0.0	0.0	0.26286623226493494	0.0
5	0.0	0.0	0.0	0.5727427279279478	0.0
6	0.0	0.0	0.0	0.6986128610055061	0.0
7	0.0	0.0	0.0	1.0244752914911424	0.0
8	0.0	0.0	0.0	1.4040419045453238	0.0
9	0.0	0.0	0.0	1.7556407731201549	0.0
10	0.0	0.0	0.0	1.9853736337833237	0.0
11	0.0	0.0	0.0	2.1980507551902324	0.0
12	0.0	0.0	0.0	2.3740488674438947	0.0
13	0.0	0.0	0.0	2.4655629952812457	0.0
14	0.0	0.0	0.0	2.532593884508804	0.0
15	0.0	0.0	0.0	2.713971584771609	0.0
16	0.0	0.0	0.0	2.9193434701888297	0.0
17	0.0	0.0	0.0	3.1942220779514527	0.0
18	0.0	0.0	0.0	3.345033937253219	0.0
19	0.0	0.0	0.0	3.5313021720697906	0.0
20	0.0	0.0	0.0	3.7251507354446995	0.0
21	0.0	0.0	0.0	3.9294833822692445	0.0
22	0.0	0.0	0.0	4.139654104717349	0.0
23	0.0	0.0	0.0	4.331485322588829	0.0
24	2.445267276883116E-4	0.0	0.0	4.498588775122829	0.0
25	3.0565840961038944E-4	0.0	0.0	4.669146167685426	0.0
26	3.0565840961038944E-4	0.0	0.0	4.844655226483712	0.0
27	3.0565840961038944E-4	0.0	0.0	5.035172113193868	0.0
28	3.973559324935063E-4	0.0	0.0	5.224466366265582	0.0
29	3.973559324935063E-4	0.0	0.0	5.434270298622153	0.0
30	3.973559324935063E-4	0.0	0.0	5.633895805938699	0.0
31	3.973559324935063E-4	0.0	0.0	5.841254471018386	0.0
32	3.973559324935063E-4	0.0	0.0	6.082969141338283	0.0
33	3.973559324935063E-4	0.0	0.0	6.318387248420205	0.0
34	3.973559324935063E-4	0.0	0.0	6.59039266713249	0.0
35	3.973559324935063E-4	0.0	0.0	6.832046205770464	0.0
36	3.973559324935063E-4	0.0	0.0	7.0899302059587495	0.0
37	3.973559324935063E-4	0.0	0.0	7.456292375717762	0.0
38	3.973559324935063E-4	0.0	0.0	7.812842910528282	0.0
39	3.973559324935063E-4	0.0	0.0	8.26261926026997	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTATC	20	7.035198E-4	45.000004	22
TCGCTTA	25	3.8923907E-5	45.0	43
CGTTTTT	32190	0.0	43.671947	1
ACTACGG	155	0.0	43.548386	1
GCGATAG	315	0.0	41.42857	8
CACGACG	750	0.0	41.4	25
CGTTTTA	1755	0.0	40.897438	1
CGACGGT	775	0.0	40.35484	27
AATGCGG	410	0.0	40.060974	1
ACGTAGG	265	0.0	39.905655	1
CACTTAC	9020	0.0	39.86142	36
ACTTACT	8980	0.0	39.76336	37
CGCACTT	9080	0.0	39.59802	34
CTTACTG	9015	0.0	39.409317	38
GCACTTA	9125	0.0	39.353428	35
ATAGCGG	515	0.0	39.32039	1
ACCCGCA	9165	0.0	39.255318	31
CGGTCTA	800	0.0	39.09375	30
TAACGCC	190	0.0	39.078945	11
TTCCGTA	340	0.0	39.044117	45
>>END_MODULE