FastQCFastQC Report
Thu 26 May 2016
SRR1043412_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043412_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences5390474
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2616884.854638015135589No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC331240.6144914157827308No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT266610.4945947239519196No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC132600.24598949925368346No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114170.2117995560316217No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC103290.19161580224670408No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC61500.11409015236878983No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA61430.11396029365877657No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC56770.1053154138207512No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT694050.044.137671
CGTTTTA34450.041.8650251
CGGTCTA10850.041.47465530
CGCACTT110600.041.05334534
CCGCACT110350.041.04440333
CACTTAC111250.041.03595436
CTTACTG110200.040.95734838
ACCCGCA111000.040.8851331
CCCGCAC111900.040.81769632
ACTTACT111600.040.54435337
TCACGAC11400.040.46052624
GCACTTA112850.040.31457535
CAACGCA120200.039.30948616
CTTATGA118450.039.3013925
TATGACC118000.039.2796627
ATAGCGG8150.039.2024541
ATCAACG121300.039.10140214
AACGCAA121000.039.04958717
GCGCGAC43250.039.0173388
TTATGAC119250.039.026