FastQCFastQC Report
Thu 26 May 2016
SRR1043411_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043411_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences802702
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT468195.832675139715611No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49700.6191587911827802No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19610.2442998771648756No Hit
GCGAAGGGTCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCACAAGCCA12470.15535030434706776No Hit
GTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATT9890.12320886206836411No Hit
CTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9260.11536037034914576No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT8160.10165665464892326No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC8130.1012829169480081No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGG302.164681E-645.0000041
CTATCGG302.164681E-645.0000041
CACATCG302.164681E-645.00000416
GAATACG302.164681E-645.00000438
TTATAGT1150.045.00000436
CCGTATT302.164681E-645.00000417
GTTGTTA302.164681E-645.00000429
TTAACGT302.164681E-645.00000430
ACGTCTT302.164681E-645.00000433
ACATCGC302.164681E-645.00000417
CGCGTCA302.164681E-645.00000421
GTTATCG302.164681E-645.00000431
TCTGCGT207.032014E-445.044
CTTGTAC207.032014E-445.043
CAGCGTT253.8897513E-545.013
AACGTAG253.8897513E-545.032
GTCGATT207.032014E-445.019
GCGGTCA207.032014E-445.026
CGTTGCG207.032014E-445.016
TAACGTA253.8897513E-545.031