##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043411_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	802702
Sequences flagged as poor quality	0
Sequence length	51
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.531306761413326	31.0	31.0	33.0	30.0	34.0
2	30.567970429873103	31.0	31.0	34.0	26.0	34.0
3	31.49192103669855	31.0	31.0	34.0	28.0	34.0
4	35.094979207725906	37.0	35.0	37.0	32.0	37.0
5	35.413130900383955	37.0	35.0	37.0	33.0	37.0
6	35.80289073653734	37.0	35.0	37.0	35.0	37.0
7	33.70568280632165	37.0	35.0	37.0	32.0	37.0
8	34.75143079249834	37.0	35.0	37.0	31.0	37.0
9	36.72925693470304	39.0	37.0	39.0	32.0	39.0
10	36.68870514836141	39.0	35.0	39.0	32.0	39.0
11	36.348116735725085	38.0	35.0	39.0	32.0	39.0
12	36.17439348600103	39.0	35.0	39.0	32.0	39.0
13	36.26594551900955	39.0	35.0	39.0	32.0	39.0
14	37.64055029139083	40.0	35.0	41.0	33.0	41.0
15	37.808851852866944	40.0	35.0	41.0	33.0	41.0
16	37.778014755164435	40.0	35.0	41.0	33.0	41.0
17	37.75685746391562	40.0	36.0	41.0	33.0	41.0
18	37.487368911501406	39.0	36.0	41.0	33.0	41.0
19	37.20993469556573	38.0	35.0	41.0	33.0	41.0
20	36.91654312559331	38.0	35.0	41.0	33.0	41.0
21	36.819299067399854	38.0	35.0	41.0	32.0	41.0
22	36.725986729820036	38.0	35.0	40.0	32.0	41.0
23	36.6239463711315	37.0	35.0	40.0	32.0	41.0
24	36.45448871436722	37.0	35.0	40.0	32.0	41.0
25	36.358945162712935	37.0	35.0	40.0	32.0	41.0
26	36.093886647846894	36.0	35.0	40.0	31.0	41.0
27	36.03194211550488	36.0	35.0	40.0	31.0	41.0
28	35.924987106049315	36.0	35.0	40.0	31.0	41.0
29	35.86502338352216	36.0	35.0	40.0	31.0	41.0
30	35.70810338083124	36.0	35.0	40.0	31.0	41.0
31	35.288358568933425	36.0	35.0	40.0	29.0	41.0
32	35.13815961589731	36.0	34.0	40.0	27.0	41.0
33	35.040843301748346	36.0	34.0	40.0	27.0	41.0
34	34.880260669588466	36.0	34.0	40.0	26.0	41.0
35	34.66240896372502	36.0	34.0	40.0	24.0	41.0
36	34.48254022040558	35.0	34.0	40.0	23.0	41.0
37	34.42322555568567	35.0	34.0	40.0	23.0	41.0
38	34.34497360166039	35.0	34.0	40.0	23.0	41.0
39	34.180411908778105	35.0	34.0	40.0	23.0	41.0
40	34.123708424795254	35.0	34.0	40.0	22.0	41.0
41	34.05043714853084	35.0	34.0	40.0	22.0	41.0
42	33.96692421346901	35.0	33.0	40.0	22.0	41.0
43	33.79506965224953	35.0	33.0	40.0	21.0	41.0
44	33.61618010170648	35.0	33.0	40.0	20.0	41.0
45	33.43526863020149	35.0	33.0	40.0	20.0	41.0
46	33.442130454390295	35.0	33.0	40.0	20.0	41.0
47	33.45175046281185	35.0	33.0	40.0	20.0	41.0
48	33.38858131660317	35.0	33.0	40.0	20.0	41.0
49	33.29725477200754	35.0	33.0	40.0	20.0	41.0
50	33.17508116337071	35.0	33.0	39.0	20.0	41.0
51	32.76559918874003	35.0	33.0	39.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	0.0
12	6.0
13	11.0
14	18.0
15	47.0
16	105.0
17	229.0
18	458.0
19	826.0
20	1486.0
21	2233.0
22	3664.0
23	5589.0
24	8703.0
25	12902.0
26	16153.0
27	17536.0
28	17836.0
29	18432.0
30	21092.0
31	24824.0
32	30606.0
33	40929.0
34	72461.0
35	106789.0
36	89608.0
37	72015.0
38	92268.0
39	145792.0
40	82.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.66282381257303	18.59967958221108	25.257816724014642	30.479679881201243
2	30.282844692052592	21.229048887382866	29.326823653111617	19.161282767452928
3	22.225059860321764	21.661463407341703	37.74377041542191	18.369706316914623
4	22.297564974299306	25.456769760135145	34.473440953180635	17.77222431238492
5	18.783309372594065	32.13645910935814	32.21992719589586	16.860304322151933
6	79.20760132651968	2.229968282127116	17.14372207867926	1.4187083126739437
7	75.67901911294602	7.398511527316488	15.418798009722162	1.5036713500153234
8	75.44742631761227	3.8741650076865386	18.140978843954546	2.537429830746653
9	40.30262787435437	24.37554659138759	24.897658159566067	10.424167374691978
10	30.83049500312694	23.245862100754703	34.45674733587309	11.466895560245272
11	29.821403210656012	21.506362261461913	35.39981213451567	13.272422393366403
12	17.908389414751678	34.2415989993796	33.38237602497565	14.467635560893083
13	12.677805711210388	36.79659450206926	33.793736654449596	16.731863132270757
14	11.729259426287713	25.73258818341053	46.072390501082594	16.465761889219163
15	13.033105685547064	22.08254121703945	45.715097259007706	19.169255838405785
16	14.211625235766201	23.821667318631324	35.562388034413765	26.40431941118871
17	16.766122421521313	24.248724931543713	40.41225759995615	18.57289504697883
18	20.796758946657665	26.621959332354972	34.01150115484949	18.56978056613787
19	22.495147638849787	26.5948010594218	34.50757566319755	16.402475638530863
20	16.26057989141674	30.887552292133318	34.32706035365553	18.524807462794413
21	17.94065543626402	26.918333329180694	32.788382238987815	22.35262899556747
22	13.111839761206525	31.026956454574673	33.816036337270866	22.045167446947932
23	13.115078821281124	26.331191401042975	45.80840710500285	14.745322672673048
24	14.244389574213095	27.95196723067838	41.186268378551446	16.617374816557078
25	13.966951620900408	33.00228478314493	34.3707876646626	18.65997593129206
26	12.628846072390502	34.17569658478489	35.283704288764696	17.91175305405991
27	13.148964372830765	31.004158454818846	39.68309534547067	16.163781826879713
28	12.393640479281228	27.028835109417944	39.23946869448438	21.338055716816452
29	13.990995412992618	32.54856721423393	36.56923740067921	16.89119997209425
30	20.00717576385757	32.24845584039905	32.31734815660108	15.427020239142298
31	19.905643688442286	29.653470403711463	33.1050626508966	17.335823256949652
32	18.341302251645068	31.95270473974152	32.328684866862176	17.377308141751236
33	14.536527877095113	30.95470049906441	33.4783020348772	21.03046958896328
34	14.71492533966528	30.679504971957215	34.910215746316815	19.695353942060688
35	22.236521149816497	29.942120488051604	31.025087766070097	16.796270596061802
36	15.366973048528596	33.75287466581621	34.207339710129034	16.672812575526162
37	15.925461752929483	33.2572984744027	32.967651756193455	17.849588016474357
38	15.795899349945557	34.90772416164405	32.43333142311842	16.863045065291978
39	19.392377245852135	29.752386315220342	31.704044589399306	19.151191849528217
40	14.279271759631843	28.55667483075911	34.64374076556431	22.520312644044736
41	15.92633380756495	30.42299632989578	30.642629518800252	23.008040343739022
42	16.029485413017532	29.876467231924174	32.785890654315054	21.30815670074324
43	15.40285186781645	30.736437681729956	33.90909702479874	19.951613425654852
44	13.070977772573134	38.30001669361731	29.528268273904885	19.100737259904673
45	16.695485996048344	34.241474420145956	31.288822003682565	17.774217580123132
46	15.306053803279424	33.579709531058846	31.044522126517688	20.06971453914404
47	14.786682978240991	31.176576114174377	34.69992599993522	19.336814907649412
48	16.104232953200565	29.220931304518988	35.467084920680406	19.207750821600044
49	16.894065294467936	32.74104213020523	31.915704707350923	18.449187867975912
50	14.53578040169328	32.521035203599844	31.422993838311104	21.520190556395775
51	14.459662489940229	29.41203584892027	35.22166383041278	20.90663783072672
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	766.0
1	4863.0
2	8960.0
3	33008.5
4	57057.0
5	33266.5
6	9476.0
7	9482.5
8	9489.0
9	11555.0
10	13621.0
11	15115.0
12	16609.0
13	16606.5
14	16604.0
15	15068.0
16	13532.0
17	11543.0
18	9554.0
19	8402.0
20	7250.0
21	6683.5
22	6117.0
23	5642.0
24	5167.0
25	5281.5
26	6535.5
27	7675.0
28	8272.5
29	8870.0
30	11018.0
31	13166.0
32	15272.5
33	17379.0
34	21310.0
35	25241.0
36	25733.0
37	26225.0
38	27847.0
39	29469.0
40	35974.0
41	42479.0
42	52901.5
43	63324.0
44	64993.5
45	66663.0
46	66035.5
47	65408.0
48	59669.5
49	53931.0
50	48077.0
51	42223.0
52	39536.5
53	36850.0
54	32704.5
55	28559.0
56	26768.5
57	24978.0
58	23209.5
59	21441.0
60	17924.5
61	14408.0
62	11942.5
63	9477.0
64	7906.5
65	6336.0
66	5506.5
67	4677.0
68	4027.0
69	3377.0
70	3147.5
71	2918.0
72	3174.5
73	3431.0
74	2897.5
75	1737.5
76	1111.0
77	856.0
78	601.0
79	463.5
80	326.0
81	205.5
82	85.0
83	69.0
84	53.0
85	33.0
86	13.0
87	15.5
88	18.0
89	14.5
90	11.0
91	8.5
92	6.0
93	5.5
94	5.0
95	5.0
96	5.0
97	3.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	802702.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.99019285561232
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.54304584480836	25.12607184042619
2	9.119186709566069	5.834490830507106
3	3.392795781801373	3.2560857413860167
4	1.7586991049555623	2.2504449417008487
5	1.134320066723611	1.8143558847239678
6	0.7940790837727726	1.5241645819499356
7	0.5778567479241011	1.2940024166306265
8	0.4490631625870301	1.1492493740408227
9	0.3459227012472064	0.9959520533429248
>10	2.8744889600825876	20.233692257673248
>50	0.7717011394835513	17.587976342538642
>100	0.23279919543611644	10.892758509376664
>500	0.004430434515220209	0.9546190345036337
>1k	0.0012083003223327842	1.0537015068390765
>5k	0.0	0.0
>10k+	4.027667741109281E-4	6.032434684360323
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	46819	5.832675139715611	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4970	0.6191587911827802	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1961	0.2442998771648756	No Hit
GCGAAGGGTCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCACAAGCCA	1247	0.15535030434706776	No Hit
GTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATT	989	0.12320886206836411	No Hit
CTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	926	0.11536037034914576	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	816	0.10165665464892326	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	813	0.1012829169480081	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.08159939803314306	0.0
2	0.0	0.0	0.0	0.2708352539298519	0.0
3	0.0	0.0	0.0	0.44487244332267767	0.0
4	0.0	0.0	0.0	0.5659634584191892	0.0
5	0.0	0.0	0.0	1.2188832219179722	0.0
6	0.0	0.0	0.0	1.420078684243966	0.0
7	0.0	0.0	0.0	1.9717155307947407	0.0
8	0.0	0.0	0.0	2.6055746715468504	0.0
9	0.0	0.0	0.0	3.0678882075789025	0.0
10	0.0	0.0	0.0	3.38382114408585	0.0
11	1.2457923363838635E-4	0.0	0.0	3.755316418795518	0.0
12	1.2457923363838635E-4	0.0	0.0	4.046707246275703	0.0
13	1.2457923363838635E-4	0.0	0.0	4.183246086343376	0.0
14	1.2457923363838635E-4	0.0	0.0	4.291505440375133	0.0
15	1.2457923363838635E-4	0.0	0.0	4.660384551178395	0.0
16	1.2457923363838635E-4	0.0	0.0	5.107623999940202	0.0
17	1.2457923363838635E-4	0.0	0.0	5.633846682828746	0.0
18	1.2457923363838635E-4	0.0	0.0	5.892348592628397	0.0
19	1.2457923363838635E-4	0.0	0.0	6.168291595137424	0.0
20	1.2457923363838635E-4	0.0	0.0	6.476002302224238	0.0
21	8.720546354687045E-4	0.0	0.0	6.81398576308518	0.0
22	8.720546354687045E-4	0.0	0.0	7.166171256580898	0.0
23	9.966338691070908E-4	0.0	0.0	7.460053668733852	0.0
24	0.003239060074598045	0.0	0.0	7.692643097936719	0.0
25	0.0033636393082364315	0.0	0.0	7.92797327027963	0.0
26	0.0033636393082364315	0.0	0.0	8.171774830509953	0.0
27	0.0033636393082364315	0.0	0.0	8.411839013731123	0.0
28	0.0033636393082364315	0.0	0.0	8.668845972727114	0.0
29	0.0033636393082364315	0.0	0.0	8.98577554310317	0.0
30	0.0033636393082364315	0.0	0.0	9.27367815204148	0.0
31	0.0033636393082364315	0.0	0.0	9.512371963692628	0.0
32	0.0033636393082364315	0.0	0.0	9.774112933566878	0.0
33	0.0033636393082364315	0.0	0.0	10.046193979833113	0.0
34	0.0033636393082364315	0.0	0.0	10.387042763067738	0.0
35	0.0033636393082364315	0.0	0.0	10.6556355907921	0.0
36	0.0033636393082364315	0.0	0.0	10.942167828160388	0.0
37	0.0033636393082364315	0.0	0.0	11.237046874182449	0.0
38	0.0033636393082364315	0.0	0.0	11.537531985718237	0.0
39	0.0033636393082364315	0.0	0.0	11.837269621852194	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCTCGG	30	2.164681E-6	45.000004	1
CTATCGG	30	2.164681E-6	45.000004	1
CACATCG	30	2.164681E-6	45.000004	16
GAATACG	30	2.164681E-6	45.000004	38
TTATAGT	115	0.0	45.000004	36
CCGTATT	30	2.164681E-6	45.000004	17
GTTGTTA	30	2.164681E-6	45.000004	29
TTAACGT	30	2.164681E-6	45.000004	30
ACGTCTT	30	2.164681E-6	45.000004	33
ACATCGC	30	2.164681E-6	45.000004	17
CGCGTCA	30	2.164681E-6	45.000004	21
GTTATCG	30	2.164681E-6	45.000004	31
TCTGCGT	20	7.032014E-4	45.0	44
CTTGTAC	20	7.032014E-4	45.0	43
CAGCGTT	25	3.8897513E-5	45.0	13
AACGTAG	25	3.8897513E-5	45.0	32
GTCGATT	20	7.032014E-4	45.0	19
GCGGTCA	20	7.032014E-4	45.0	26
CGTTGCG	20	7.032014E-4	45.0	16
TAACGTA	25	3.8897513E-5	45.0	31
>>END_MODULE