FastQCFastQC Report
Thu 26 May 2016
SRR1043410_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043410_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences614093
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT421156.858081756346351No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43910.7150382759614586No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17750.2890441675772236No Hit
GTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATT10370.16886693057891883No Hit
GCGAAGGGTCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCACAAGCCA9800.15958494885953756No Hit
CTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8150.13271605440869705No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC7280.11854881915279933No Hit
CCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGC6830.11122093884802466No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAC2150.045.024
CAGGTTT253.8886763E-545.045
CAGGTTC253.8886763E-545.031
CAGGTAT453.8380676E-1045.040
TGTCACC453.8380676E-1045.030
CGGGTAC502.1827873E-1145.05
ATCTCGG207.0307165E-445.01
ACCGGGT406.8066583E-945.03
ACCGGCA207.0307165E-445.03
AGTTTGG800.045.01
TCCGCAA551.8189894E-1245.031
GGTAACC207.0307165E-445.018
GCAACTA207.0307165E-445.08
ACACCCG351.210683E-745.028
TATGGGT700.045.03
CACATCG502.1827873E-1145.016
ACCGACT351.210683E-745.033
TCGGGCA502.1827873E-1145.04
CCTACGG253.8886763E-545.01
CACGACC207.0307165E-445.026