##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043410_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	614093
Sequences flagged as poor quality	0
Sequence length	51
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.52129074912106	31.0	31.0	33.0	30.0	34.0
2	30.565984305308806	31.0	31.0	34.0	26.0	34.0
3	31.451099426308392	31.0	31.0	34.0	28.0	34.0
4	35.06343176033597	37.0	35.0	37.0	32.0	37.0
5	35.375421963774215	37.0	35.0	37.0	33.0	37.0
6	35.7983188214163	37.0	35.0	37.0	35.0	37.0
7	33.777701423074355	37.0	35.0	37.0	32.0	37.0
8	34.79456206144672	37.0	35.0	37.0	31.0	37.0
9	36.78498696451515	39.0	37.0	39.0	32.0	39.0
10	36.714427619269394	39.0	35.0	39.0	32.0	39.0
11	36.3137993756646	38.0	35.0	39.0	32.0	39.0
12	36.11398762076754	38.0	35.0	39.0	32.0	39.0
13	36.21258017922367	39.0	35.0	39.0	32.0	39.0
14	37.5718563800597	40.0	35.0	41.0	33.0	41.0
15	37.74612477263216	40.0	35.0	41.0	33.0	41.0
16	37.7047515604314	40.0	35.0	41.0	33.0	41.0
17	37.687084855225514	40.0	36.0	41.0	33.0	41.0
18	37.40123238662548	39.0	36.0	41.0	33.0	41.0
19	37.120996331174595	38.0	35.0	41.0	33.0	41.0
20	36.781570543875276	38.0	35.0	40.0	32.0	41.0
21	36.698206949110315	38.0	35.0	40.0	32.0	41.0
22	36.61039125995574	37.0	35.0	40.0	32.0	41.0
23	36.514488847780385	37.0	35.0	40.0	32.0	41.0
24	36.34951546427007	37.0	35.0	40.0	32.0	41.0
25	36.23860718164838	36.0	35.0	40.0	32.0	41.0
26	35.959763423455406	36.0	35.0	40.0	31.0	41.0
27	35.89868635532403	36.0	35.0	40.0	31.0	41.0
28	35.787628258260554	36.0	35.0	40.0	31.0	41.0
29	35.75517063376394	36.0	35.0	40.0	31.0	41.0
30	35.56107462550461	35.0	35.0	40.0	31.0	41.0
31	35.12196198295698	35.0	34.0	40.0	29.0	41.0
32	34.981338331490505	35.0	34.0	40.0	27.0	41.0
33	34.88685589967643	35.0	34.0	40.0	27.0	41.0
34	34.739155144253395	35.0	34.0	40.0	26.0	41.0
35	34.516120522461584	35.0	34.0	40.0	24.0	41.0
36	34.347919614781475	35.0	34.0	40.0	23.0	41.0
37	34.29738003852837	35.0	34.0	40.0	23.0	41.0
38	34.19469526602648	35.0	34.0	40.0	23.0	41.0
39	34.01630209105136	35.0	33.0	40.0	23.0	41.0
40	33.95120934451297	35.0	33.0	40.0	22.0	41.0
41	33.87232552724099	35.0	33.0	40.0	22.0	41.0
42	33.75197567795106	35.0	33.0	40.0	22.0	41.0
43	33.57287739804883	35.0	33.0	40.0	21.0	41.0
44	33.34710866269441	35.0	33.0	40.0	20.0	41.0
45	33.18003299174555	35.0	33.0	39.0	20.0	41.0
46	33.18073646825481	35.0	33.0	39.0	20.0	41.0
47	33.15563440716634	35.0	33.0	39.0	20.0	41.0
48	33.0980942625954	35.0	33.0	39.0	20.0	41.0
49	32.96239494669374	35.0	33.0	39.0	20.0	41.0
50	32.816244445059624	35.0	33.0	39.0	20.0	41.0
51	32.38682740236414	35.0	32.0	38.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	0.0
10	0.0
11	1.0
12	3.0
13	9.0
14	16.0
15	24.0
16	85.0
17	157.0
18	336.0
19	620.0
20	1168.0
21	1753.0
22	2773.0
23	4112.0
24	6715.0
25	9844.0
26	12353.0
27	13566.0
28	13933.0
29	14745.0
30	16734.0
31	19870.0
32	24298.0
33	32363.0
34	58735.0
35	87246.0
36	72085.0
37	53508.0
38	66645.0
39	100336.0
40	59.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.0799260046931	18.713452196979937	24.292086052112627	30.914535746214334
2	32.041400895304136	21.1523336041935	29.145911124210826	17.66035437629154
3	22.62540038723776	21.189950056424678	38.25479202661486	17.9298575297227
4	21.437958094295166	25.25692362557463	35.30100489665246	18.004113383477748
5	18.30439363418896	32.45143650880241	33.47880532753182	15.765364529476805
6	77.75564938861052	2.3480156914343593	18.411217844854118	1.4851170751010025
7	74.80397920184728	7.099576122834815	16.48203122328377	1.6144134520341382
8	74.68152217986527	3.494421854670221	19.404389888827914	2.4196660766366005
9	40.744154387039096	24.08250867539607	24.95713190021707	10.216205037347763
10	31.426184633272157	22.423802257964184	34.69669903418537	11.45331407457828
11	29.771874944023136	21.42232528297831	35.37770337717577	13.428096395822783
12	16.351432112074228	35.47882812538166	34.1984031734607	13.971336589083414
13	12.190173149669512	37.46598642225201	35.4164597218988	14.927380706179683
14	10.522347592302793	26.298459679559937	48.36270727723651	14.81648545090076
15	11.88012239188527	23.129558552206262	47.72974126068853	17.26057779521994
16	13.417023154473345	23.964122698027822	35.75875315302405	26.860100994474777
17	15.987969248957407	24.873594064742637	41.335432906742135	17.80300377955782
18	19.736424287526482	27.069352687622235	34.96050272515727	18.23372029969402
19	21.440726404632525	26.57252240295851	34.76297564049745	17.22377555191152
20	14.946433194972098	31.32799103718818	36.283755066415026	17.441820701424703
21	17.616061410893792	26.963342685879827	33.28534928748577	22.135246615740613
22	12.639616475029026	31.106851893768532	34.88282719392665	21.370704437275787
23	12.240328419311082	25.756847904144813	46.68169153532119	15.32113214122291
24	12.751163097446153	27.081077296109875	42.56684248151338	17.600917124930586
25	12.773635263714128	33.251803879868355	35.61463817369682	18.359922682720693
26	11.924415357283017	35.438280521028574	35.087030791753044	17.55027332993537
27	12.634242696138859	30.870894147954786	40.69725595308854	15.797607202817815
28	11.389968620388117	27.100943993825037	39.99801333022848	21.51107405555836
29	14.37420716406147	32.64652096669397	36.60878726837792	16.370484600866643
30	20.891949590697177	30.985371922493826	33.07251507507821	15.050163411730797
31	19.983129591120562	29.73523554249926	33.98068370751661	16.300951158863562
32	19.42800194758774	31.76473270335275	32.11223707158362	16.69502827747589
33	15.754128446342818	30.510850962313523	33.1591469044591	20.57587368688456
34	16.021514656574816	29.39603610528047	35.04192361743254	19.540525620712174
35	22.68695458179787	29.47664278863299	31.354534248069914	16.48186838149922
36	15.773018093350682	34.03214171143459	33.799929326665506	16.394910868549225
37	17.021200371930636	33.3234542650706	32.329630853958605	17.32571450904016
38	16.42275681370737	34.266796722971925	31.85999514731482	17.450451316005882
39	19.91701582659304	29.419648164040304	31.086333828915162	19.577002180451494
40	14.393911019992087	28.525646766857786	34.615278141910096	22.465164071240025
41	16.979675716870247	30.07866886611637	30.605787722706495	22.335867694306888
42	16.575013882262134	29.80281488308774	32.73820089139593	20.883970343254198
43	16.41380051555709	31.21872419975476	33.56771694189643	18.799758342791726
44	14.19915224566963	38.10742021159662	29.178479481120938	18.514948061612817
45	18.227206628311997	33.758893196958766	30.96908774403877	17.044812430690463
46	16.992214534280638	32.51869016582179	30.599925418462675	19.889169881434896
47	16.390839823935462	31.525843805417097	34.242044771720245	17.841271598927197
48	17.819450799797426	28.9599458062541	34.31402084049159	18.90658255345689
49	17.641464729283676	32.71963692795717	31.75023978452775	17.888658558231405
50	15.519799118374578	31.956560325553298	31.76180155123084	20.76183900484129
51	15.480717090082447	28.804269060223774	35.04794876346091	20.667065086232867
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	832.0
1	4224.5
2	7617.0
3	28648.0
4	49679.0
5	28387.0
6	7095.0
7	7263.0
8	7431.0
9	9141.0
10	10851.0
11	12362.0
12	13873.0
13	13884.5
14	13896.0
15	12322.0
16	10748.0
17	9304.5
18	7861.0
19	6756.5
20	5652.0
21	5269.5
22	4887.0
23	4531.5
24	4176.0
25	4336.0
26	5195.0
27	5894.0
28	6037.0
29	6180.0
30	7323.5
31	8467.0
32	9606.5
33	10746.0
34	13036.5
35	15327.0
36	16990.5
37	18654.0
38	19255.0
39	19856.0
40	25216.0
41	30576.0
42	36416.5
43	42257.0
44	46133.0
45	50009.0
46	49543.0
47	49077.0
48	47273.0
49	45469.0
50	42197.0
51	38925.0
52	33660.5
53	28396.0
54	24899.5
55	21403.0
56	19875.5
57	18348.0
58	16752.0
59	15156.0
60	12478.0
61	9800.0
62	8710.5
63	7621.0
64	6732.0
65	5843.0
66	4826.5
67	3810.0
68	3607.0
69	3404.0
70	3159.5
71	2915.0
72	2763.5
73	2612.0
74	2278.0
75	1488.5
76	1033.0
77	805.5
78	578.0
79	490.5
80	403.0
81	288.5
82	174.0
83	129.0
84	84.0
85	51.0
86	18.0
87	12.0
88	6.0
89	6.5
90	7.0
91	5.0
92	3.0
93	3.0
94	3.0
95	2.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	614093.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.75437931222399
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.74078053631852	26.5784617356348
2	9.154009927510092	6.179758466420795
3	3.3981960170012373	3.4411199200544558
4	1.8435144536716352	2.4890674454719908
5	1.1879084517107075	2.0048556233619967
6	0.8135712349611463	1.6476955237435826
7	0.5968536789939116	1.4102497832259806
8	0.4260551828648376	1.1504982600286948
9	0.3513058395023578	1.0672299505045681
>10	2.6010291812372337	19.253029866883402
>50	0.6863466354491103	16.36109576142423
>100	0.1940737993399123	9.273317203676442
>500	0.004399657919544612	1.0057272968630742
>1k	0.0014665526398482038	1.1885568038235805
>5k	0.0	0.0
>10k+	4.888508799494013E-4	6.949336358882423
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	42115	6.858081756346351	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4391	0.7150382759614586	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1775	0.2890441675772236	No Hit
GTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATT	1037	0.16886693057891883	No Hit
GCGAAGGGTCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCACAAGCCA	980	0.15958494885953756	No Hit
CTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	815	0.13271605440869705	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	728	0.11854881915279933	No Hit
CCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGC	683	0.11122093884802466	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.10845262851066532	0.0
2	0.0	0.0	0.0	0.37258200305165506	0.0
3	0.0	0.0	0.0	0.5907899943493901	0.0
4	0.0	0.0	0.0	0.7419071704122991	0.0
5	0.0	0.0	0.0	1.63574572581026	0.0
6	0.0	0.0	0.0	1.8725176805467576	0.0
7	0.0	0.0	0.0	2.539354788281254	0.0
8	0.0	0.0	0.0	3.3691965223508493	0.0
9	0.0	0.0	0.0	3.9129252409651305	0.0
10	0.0	0.0	0.0	4.317587075573244	0.0
11	0.0	0.0	0.0	4.795853396798205	0.0
12	0.0	0.0	0.0	5.1894419900568804	0.0
13	0.0	0.0	0.0	5.351632407469227	0.0
14	0.0	0.0	0.0	5.478974682987756	0.0
15	0.0	0.0	0.0	5.92564969800991	0.0
16	0.0	0.0	0.0	6.470029783762394	0.0
17	0.0	0.0	0.0	7.158850532411215	0.0
18	0.0	0.0	0.0	7.481440107605851	0.0
19	0.0	0.0	0.0	7.840831926108912	0.0
20	0.0	0.0	0.0	8.22937242404652	0.0
21	0.0	0.0	0.0	8.588764242549582	0.0
22	1.6284178455054855E-4	0.0	0.0	8.976490531564437	0.0
23	3.256835691010971E-4	0.0	0.0	9.26537185735711	0.0
24	8.142089227527427E-4	0.0	0.0	9.51728809805681	0.0
25	8.142089227527427E-4	0.0	0.0	9.765784661280946	0.0
26	8.142089227527427E-4	0.0	0.0	10.036264865419406	0.0
27	8.142089227527427E-4	0.0	0.0	10.297137404269385	0.0
28	8.142089227527427E-4	0.0	0.0	10.568106133761498	0.0
29	8.142089227527427E-4	0.0	0.0	10.920170071959785	0.0
30	8.142089227527427E-4	0.0	0.0	11.231686405804984	0.0
31	8.142089227527427E-4	0.0	0.0	11.492396102870412	0.0
32	8.142089227527427E-4	0.0	0.0	11.753757167074042	0.0
33	8.142089227527427E-4	0.0	0.0	12.010884344879358	0.0
34	8.142089227527427E-4	0.0	0.0	12.371090372305172	0.0
35	8.142089227527427E-4	0.0	0.0	12.657203387760486	0.0
36	8.142089227527427E-4	0.0	0.0	12.935825681126474	0.0
37	8.142089227527427E-4	0.0	0.0	13.238711400390494	0.0
38	8.142089227527427E-4	0.0	0.0	13.538340283963503	0.0
39	8.142089227527427E-4	0.0	0.0	13.851485035654209	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	215	0.0	45.0	24
CAGGTTT	25	3.8886763E-5	45.0	45
CAGGTTC	25	3.8886763E-5	45.0	31
CAGGTAT	45	3.8380676E-10	45.0	40
TGTCACC	45	3.8380676E-10	45.0	30
CGGGTAC	50	2.1827873E-11	45.0	5
ATCTCGG	20	7.0307165E-4	45.0	1
ACCGGGT	40	6.8066583E-9	45.0	3
ACCGGCA	20	7.0307165E-4	45.0	3
AGTTTGG	80	0.0	45.0	1
TCCGCAA	55	1.8189894E-12	45.0	31
GGTAACC	20	7.0307165E-4	45.0	18
GCAACTA	20	7.0307165E-4	45.0	8
ACACCCG	35	1.210683E-7	45.0	28
TATGGGT	70	0.0	45.0	3
CACATCG	50	2.1827873E-11	45.0	16
ACCGACT	35	1.210683E-7	45.0	33
TCGGGCA	50	2.1827873E-11	45.0	4
CCTACGG	25	3.8886763E-5	45.0	1
CACGACC	20	7.0307165E-4	45.0	26
>>END_MODULE