##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043409_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1089531
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.56498805449317	31.0	31.0	33.0	30.0	34.0
2	30.604792337253368	31.0	31.0	34.0	26.0	34.0
3	31.517084874133918	31.0	31.0	34.0	28.0	34.0
4	35.12599182584066	37.0	35.0	37.0	32.0	37.0
5	35.43848224603063	37.0	35.0	37.0	33.0	37.0
6	35.86535215611121	37.0	35.0	37.0	35.0	37.0
7	33.85798751940055	37.0	35.0	37.0	32.0	37.0
8	34.849397584832374	37.0	35.0	37.0	32.0	37.0
9	36.82117902106503	39.0	37.0	39.0	32.0	39.0
10	36.70029948665986	39.0	35.0	39.0	32.0	39.0
11	36.544981280936476	39.0	35.0	39.0	32.0	39.0
12	36.475387116107754	39.0	35.0	39.0	32.0	39.0
13	36.528811938347786	39.0	35.0	39.0	32.0	39.0
14	37.87142082235384	40.0	36.0	41.0	33.0	41.0
15	38.01170687203944	40.0	36.0	41.0	33.0	41.0
16	37.97032851749973	40.0	36.0	41.0	33.0	41.0
17	37.94397956551948	40.0	36.0	41.0	33.0	41.0
18	37.68682763500993	39.0	36.0	41.0	33.0	41.0
19	37.430092397554546	38.0	35.0	41.0	33.0	41.0
20	37.13268277818621	38.0	35.0	41.0	33.0	41.0
21	37.03589159005113	38.0	35.0	41.0	33.0	41.0
22	36.95101378483035	38.0	35.0	40.0	33.0	41.0
23	36.87343269718806	38.0	35.0	40.0	33.0	41.0
24	36.72679712647001	38.0	35.0	40.0	32.0	41.0
25	36.63542386586522	38.0	35.0	40.0	32.0	41.0
26	36.38257103285726	38.0	35.0	40.0	32.0	41.0
27	36.308359284866604	37.0	35.0	40.0	31.0	41.0
28	36.24798560114398	37.0	35.0	40.0	31.0	41.0
29	36.18959258616781	37.0	35.0	40.0	31.0	41.0
30	35.996053347724846	37.0	35.0	40.0	31.0	41.0
31	35.57874259658514	37.0	35.0	40.0	30.0	41.0
32	35.4178954063721	37.0	35.0	40.0	28.0	41.0
33	35.27333320483768	37.0	35.0	40.0	27.0	41.0
34	35.065625484726915	37.0	35.0	40.0	25.0	41.0
35	34.844704739929384	37.0	35.0	40.0	24.0	41.0
36	34.65609239204759	37.0	34.0	40.0	23.0	41.0
37	34.55834207562704	37.0	34.0	40.0	23.0	41.0
38	34.45554004429429	37.0	34.0	40.0	22.0	41.0
39	34.3379977256269	37.0	34.0	40.0	22.0	41.0
40	34.260788357559356	37.0	34.0	40.0	21.0	41.0
41	34.167271055160434	36.0	34.0	40.0	21.0	41.0
42	34.08722652223755	36.0	34.0	40.0	21.0	41.0
43	33.940839682395456	36.0	33.0	40.0	20.0	41.0
44	33.746972780031044	36.0	33.0	40.0	18.0	41.0
45	33.555452759031176	35.0	33.0	40.0	18.0	41.0
46	33.55625034992121	35.0	33.0	40.0	18.0	41.0
47	33.53298162236779	35.0	33.0	40.0	18.0	41.0
48	33.487514352505805	36.0	33.0	40.0	18.0	41.0
49	33.40064394679913	35.0	33.0	40.0	18.0	41.0
50	33.292231244452886	35.0	33.0	40.0	18.0	41.0
51	32.871574099314294	35.0	32.0	40.0	17.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	0.0
12	4.0
13	4.0
14	24.0
15	28.0
16	92.0
17	237.0
18	526.0
19	991.0
20	1665.0
21	2810.0
22	4389.0
23	7132.0
24	11846.0
25	18233.0
26	23017.0
27	24929.0
28	24701.0
29	24822.0
30	27564.0
31	32374.0
32	39963.0
33	52004.0
34	88398.0
35	124886.0
36	111373.0
37	103721.0
38	139591.0
39	224103.0
40	102.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.1995298894662	18.364690862398593	25.947953752577945	29.487825495557264
2	31.736683031506217	20.85245853491089	29.658082239055155	17.752776194527737
3	21.835358516646153	21.55165846589037	37.771389708048694	18.841593309414783
4	22.90976576159834	24.908056769380586	34.27419687920766	17.907980589813416
5	19.982542947378278	31.47556150306875	32.42688826660279	16.115007282950184
6	79.48337403892134	1.841159177664518	17.26054605146618	1.4149207319479666
7	76.2915419570439	6.834500349232835	15.523560137343498	1.3503975563797632
8	75.8104175099194	3.9242573180570353	18.198013640731656	2.0673115312919044
9	37.28044452154184	30.362697344086587	23.39410260010959	8.96275553426199
10	26.92268508192975	23.09369811414269	38.43782324688329	11.545793557044268
11	25.69022818074933	21.690709121631237	38.47710620441273	14.14195649320671
12	17.757548890302342	29.86762194008248	38.06096384591168	14.313865323703501
13	14.266597279012712	31.976602776791115	37.88758649363809	15.869213450558084
14	13.308937515316224	25.667466093208912	45.41642229546475	15.607174096010118
15	15.799917579215277	23.830253567819547	44.5715633607488	15.798265492216377
16	16.221291546546173	23.327927337542484	37.88235488480824	22.568426231103107
17	16.99281617503311	23.48193855888451	41.52915336966089	17.996091896421486
18	19.754738506751988	25.247285299821666	37.60324396460496	17.39473222882139
19	21.02014536529938	25.37330282479342	37.228587346298546	16.377964463608656
20	17.51248931879864	28.053722197899834	38.111719629822375	16.32206885347916
21	18.610117564346492	24.838577332815678	36.382168107194744	20.16913699564308
22	14.794806205605898	27.86033623641732	36.869992684925904	20.474864873050883
23	14.339105541742272	24.159386011045118	45.57162669075042	15.929881756462184
24	14.997829341248666	25.971633666228865	42.11757168910293	16.912965303419544
25	14.93183764390366	30.545436522687286	37.054200385303396	17.468525448105655
26	14.426666152684046	30.29927555985098	37.745874142176774	17.528184145288204
27	14.342134367906926	27.94615297774914	41.03361905260153	16.6780936017424
28	13.712230308270257	25.088409600093986	41.21745962253483	19.981900469100925
29	15.208011520553338	29.491405017388217	38.078953237677496	17.22163022438095
30	19.204777101339936	29.04368944068595	35.5409804769208	16.21055298105332
31	19.712426723057902	27.865476062636123	36.284786756870616	16.137310457435355
32	19.474159064771907	29.283976316415046	34.027118090260856	17.214746528552194
33	15.858383102454177	28.932265350871155	35.4113834301181	19.797968116556575
34	16.281592722006074	27.635927752399887	37.09385047327704	18.988629052317005
35	21.653261816322804	28.218104854290516	32.561533357013246	17.567099972373434
36	17.416209359807112	30.557368262123795	34.43417397026794	17.592248407801154
37	16.961334739442936	30.496057477942344	34.22546031274007	18.317147469874655
38	17.16243044025365	30.560121740455298	33.30699172396196	18.97045609532909
39	19.804576464552177	28.07813637243915	32.67387527293854	19.44341189007013
40	15.882521929160346	27.60444631680971	34.526782624817464	21.986249129212478
41	16.682407384461754	29.003488657046013	32.20963882624726	22.104465132244975
42	17.21346157199749	28.509239296541356	33.242835678837956	21.034463452623193
43	17.687794105904285	29.50966975698718	33.649432645789794	19.153103491318742
44	16.20440354611296	33.430898248879565	31.282359106808343	19.08233909819913
45	18.59212817258068	30.646397394842367	32.26590156682095	18.495572865756
46	17.91523141608637	29.81585654745023	32.28921435002767	19.979697686435724
47	16.636240731103566	28.751912520157756	34.79304397947374	19.81880276926494
48	17.907613459369216	26.774180817250727	34.75807480466366	20.560130918716403
49	18.56743865020821	29.707094153355897	32.56190048745744	19.16356670897845
50	16.609348426065896	29.157316313165943	33.15206267650944	21.08127258425873
51	16.76556243007312	27.868596671411826	34.0871439178876	21.278696980627444
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	990.0
1	5678.5
2	10367.0
3	39209.0
4	68051.0
5	40569.5
6	13088.0
7	12946.5
8	12805.0
9	14543.5
10	16282.0
11	17371.0
12	18460.0
13	18381.5
14	18303.0
15	16526.5
16	14750.0
17	13394.0
18	12038.0
19	11030.0
20	10022.0
21	9847.5
22	9673.0
23	9097.5
24	8522.0
25	9207.0
26	11319.0
27	12746.0
28	13850.5
29	14955.0
30	17475.5
31	19996.0
32	23534.0
33	27072.0
34	31292.0
35	35512.0
36	37851.0
37	40190.0
38	43841.5
39	47493.0
40	53376.5
41	59260.0
42	68134.5
43	77009.0
44	81135.0
45	85261.0
46	84984.5
47	84708.0
48	82200.0
49	79692.0
50	75208.0
51	70724.0
52	63062.5
53	55401.0
54	48052.0
55	40703.0
56	35876.5
57	31050.0
58	27885.0
59	24720.0
60	20770.0
61	16820.0
62	14633.5
63	12447.0
64	10106.5
65	7766.0
66	6797.0
67	5828.0
68	5120.5
69	4413.0
70	4091.5
71	3770.0
72	3739.5
73	3709.0
74	3073.0
75	1843.5
76	1250.0
77	1008.0
78	766.0
79	557.5
80	349.0
81	235.0
82	121.0
83	101.0
84	81.0
85	52.5
86	24.0
87	15.5
88	7.0
89	5.5
90	4.0
91	2.5
92	1.0
93	1.0
94	1.0
95	1.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1089531.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.401584600550805
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.50372258109412	26.221487312505865
2	9.506768473043433	6.35082262860419
3	3.4321183545177165	3.4391457473258016
4	1.7290358781516766	2.310101526458835
5	1.0559858525212724	1.7635800394987022
6	0.7395264309344629	1.4820812788320514
7	0.5415396293152928	1.2661797220187974
8	0.3734827699283985	0.9979933069289164
9	0.2987030186077443	0.8979438731819827
>10	2.8671169926896365	22.051920175939017
>50	0.7604600901445525	17.69223605598614
>100	0.18612436558151452	8.71263266052029
>500	0.004275444844860692	0.9431902067105372
>1k	5.700593126480921E-4	0.3004651551810291
>5k	2.8502965632404607E-4	0.5314310824526314
>10k+	2.8502965632404607E-4	5.038789227855243
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	52926	4.857686472436305	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5582	0.5123305348815224	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2154	0.19769974420186298	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.08196187166771758	0.0
2	0.0	0.0	0.0	0.2841589638110343	0.0
3	0.0	0.0	0.0	0.4662556641343844	0.0
4	0.0	0.0	0.0	0.5854812758884327	0.0
5	0.0	0.0	0.0	1.2654986411584435	0.0
6	0.0	0.0	0.0	1.41905094944522	0.0
7	0.0	0.0	0.0	1.922386788443835	0.0
8	0.0	0.0	0.0	2.543663282641797	0.0
9	0.0	0.0	0.0	2.978896424241256	0.0
10	0.0	0.0	0.0	3.32317299829009	0.0
11	0.0	0.0	0.0	3.746382617841989	0.0
12	0.0	0.0	0.0	4.089007104891921	0.0
13	0.0	0.0	0.0	4.241274456623997	0.0
14	0.0	0.0	0.0	4.369311199038853	0.0
15	0.0	0.0	0.0	4.8013319492515585	0.0
16	0.0	0.0	0.0	5.361664789712271	0.0
17	0.0	0.0	0.0	6.0154323282219595	0.0
18	0.0	0.0	0.0	6.384673772476414	0.0
19	0.0	0.0	0.0	6.7720881737187835	0.0
20	0.0	0.0	0.0	7.191626488828679	0.0
21	0.0	0.0	0.0	7.642829804750852	0.0
22	0.0	0.0	0.0	8.115969164713992	0.0
23	0.0	0.0	0.0	8.504301392066862	0.0
24	6.424782773505297E-4	0.0	0.0	8.828661139517829	0.0
25	6.424782773505297E-4	0.0	0.0	9.139253495311285	0.0
26	6.424782773505297E-4	0.0	0.0	9.45994193832025	0.0
27	6.424782773505297E-4	0.0	0.0	9.78760585976902	0.0
28	9.178261105007567E-4	0.0	0.0	10.126375477154848	0.0
29	9.178261105007567E-4	0.0	0.0	10.55160431414985	0.0
30	9.178261105007567E-4	0.0	0.0	10.95122580266188	0.0
31	9.178261105007567E-4	0.0	0.0	11.30422172476047	0.0
32	9.178261105007567E-4	0.0	0.0	11.64216529864685	0.0
33	9.178261105007567E-4	0.0	0.0	12.014619134288056	0.0
34	9.178261105007567E-4	0.0	0.0	12.438930145172556	0.0
35	9.178261105007567E-4	0.0	0.0	12.835063894464682	0.0
36	9.178261105007567E-4	0.0	0.0	13.207242382272739	0.0
37	9.178261105007567E-4	0.0	0.0	13.58263326146755	0.0
38	9.178261105007567E-4	0.0	0.0	13.970231227932018	0.0
39	0.0010096087215508325	0.0	0.0	14.377103542717004	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTACCGG	65	0.0	45.000004	1
AGCGTAA	20	7.0331263E-4	45.0	21
AACCGCG	20	7.0331263E-4	45.0	44
ACGCCCC	25	3.890671E-5	45.0	16
GATCGTC	20	7.0331263E-4	45.0	9
TCCGATT	20	7.0331263E-4	45.0	23
GGCACTA	20	7.0331263E-4	45.0	7
CGTGACA	20	7.0331263E-4	45.0	32
CGACCGA	20	7.0331263E-4	45.0	33
CGACATA	20	7.0331263E-4	45.0	11
ATCACGT	45	3.8562575E-10	45.0	15
ATCGTGT	20	7.0331263E-4	45.0	34
TAACCCG	25	3.890671E-5	45.0	36
TCTACGG	100	0.0	45.0	1
CACTACG	20	7.0331263E-4	45.0	18
CTTGCGG	275	0.0	45.0	1
TTGACGT	20	7.0331263E-4	45.0	19
GATATCG	40	6.8139343E-9	45.0	8
CAAATCG	20	7.0331263E-4	45.0	10
TTAAACG	20	7.0331263E-4	45.0	29
>>END_MODULE