##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043408_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	358576
Sequences flagged as poor quality	0
Sequence length	51
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.514780688054973	31.0	31.0	34.0	30.0	34.0
2	30.529463767792603	31.0	31.0	34.0	26.0	34.0
3	31.412484382669223	31.0	31.0	34.0	28.0	34.0
4	35.02224632992727	37.0	35.0	37.0	32.0	37.0
5	35.342753000758556	37.0	35.0	37.0	33.0	37.0
6	35.79385123376913	37.0	35.0	37.0	35.0	37.0
7	33.6958775824372	37.0	35.0	37.0	32.0	37.0
8	34.7470271295346	37.0	35.0	37.0	31.0	37.0
9	36.76167674356343	39.0	37.0	39.0	32.0	39.0
10	36.68906452188657	39.0	35.0	39.0	32.0	39.0
11	36.05909765293829	37.0	35.0	39.0	32.0	39.0
12	35.69487918879122	38.0	35.0	39.0	31.0	39.0
13	35.81309401633126	38.0	35.0	39.0	31.0	39.0
14	37.21130806300477	39.0	35.0	41.0	32.0	41.0
15	37.42981961982955	39.0	35.0	41.0	33.0	41.0
16	37.41536243362634	39.0	35.0	41.0	33.0	41.0
17	37.416726161260094	39.0	35.0	41.0	33.0	41.0
18	37.1321700281112	39.0	35.0	40.0	33.0	41.0
19	36.78825688278078	37.0	35.0	40.0	32.0	41.0
20	36.402355428137966	36.0	35.0	40.0	32.0	41.0
21	36.30481404221141	36.0	35.0	40.0	32.0	41.0
22	36.22245214403641	36.0	35.0	40.0	32.0	41.0
23	36.11421009771987	35.0	35.0	40.0	32.0	41.0
24	35.937848601133375	35.0	35.0	40.0	31.0	41.0
25	35.835708469055376	35.0	35.0	40.0	31.0	41.0
26	35.53564097987596	35.0	35.0	40.0	31.0	41.0
27	35.50490551514881	35.0	35.0	40.0	31.0	41.0
28	35.432873365757885	35.0	35.0	40.0	31.0	41.0
29	35.400712819597516	35.0	35.0	40.0	31.0	41.0
30	35.19532260943287	35.0	35.0	40.0	30.0	41.0
31	34.699146066663694	35.0	34.0	40.0	29.0	41.0
32	34.569890344027485	35.0	34.0	40.0	27.0	41.0
33	34.49781636250056	35.0	34.0	40.0	26.0	41.0
34	34.333321248494045	35.0	34.0	40.0	25.0	41.0
35	34.11966779706394	35.0	34.0	40.0	23.0	41.0
36	33.918240484583464	35.0	33.0	40.0	23.0	41.0
37	33.84660992369818	35.0	33.0	40.0	23.0	41.0
38	33.80367620811209	35.0	33.0	40.0	23.0	41.0
39	33.65537849716657	35.0	33.0	39.0	22.0	41.0
40	33.60618669403418	35.0	33.0	39.0	22.0	41.0
41	33.52757853286332	35.0	33.0	39.0	22.0	41.0
42	33.4288100709473	35.0	33.0	39.0	22.0	41.0
43	33.25481627325867	35.0	33.0	39.0	21.0	41.0
44	33.02623990451118	35.0	33.0	39.0	20.0	41.0
45	32.87297532461738	35.0	33.0	38.0	20.0	41.0
46	32.88673251974477	35.0	33.0	38.0	20.0	41.0
47	32.89823914595511	35.0	33.0	38.0	20.0	41.0
48	32.86313361742002	35.0	33.0	38.0	20.0	41.0
49	32.735740819240554	35.0	33.0	38.0	20.0	41.0
50	32.61492124403195	35.0	33.0	38.0	18.0	40.0
51	32.22995682923564	35.0	32.0	38.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	5.0
14	5.0
15	22.0
16	47.0
17	99.0
18	228.0
19	473.0
20	737.0
21	1148.0
22	1701.0
23	2656.0
24	4052.0
25	5936.0
26	7504.0
27	8143.0
28	8550.0
29	9004.0
30	10306.0
31	12046.0
32	15285.0
33	20482.0
34	38510.0
35	59008.0
36	41727.0
37	28644.0
38	34625.0
39	47602.0
40	29.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.691624648610055	18.519923251974475	23.519142385435725	32.269309713979744
2	32.12512828521708	20.69937753781625	28.645252331444382	18.530241845522287
3	22.187486055954665	20.814834233189057	38.82356878318683	18.17411092766945
4	21.802909285618668	24.481839275355853	36.33204676275044	17.383204676275042
5	18.47530230690286	31.422906162152515	33.05045513363973	17.051336397304894
6	76.55197224577218	2.6401655437062157	19.21991432778546	1.5879478827361564
7	73.54117397706483	7.544565168890277	17.252409531033866	1.6618513230110215
8	73.88503413502298	3.227488733211369	20.501093213154252	2.386383918611396
9	44.867754674043994	20.974354111820087	25.02035830618892	9.13753290794699
10	37.97828075498639	20.42049663111865	32.72695328187051	8.874269332024452
11	33.56359600196332	20.864475034581233	33.315670875909156	12.256258087546295
12	15.852148498505198	39.04946231761189	32.80002007942529	12.298369104457633
13	10.83452322520191	41.523972602739725	36.55264155994824	11.088862612110125
14	9.876009548882244	24.535105528535095	54.293929320423004	11.294955602159654
15	10.74834902503235	21.212239525233144	52.59108250412744	15.448328945607068
16	11.672002588014815	21.234549997768955	36.60785997947437	30.485587434741866
17	15.219088840301637	22.816920262371156	44.2391571103476	17.72483378697961
18	21.956014903395655	24.98494043103833	35.667473562090045	17.39157110347597
19	23.00042389897818	24.477934942662085	34.74103119004061	17.780609968319126
20	14.904790058453438	31.85907590022757	36.71746017580652	16.518673865512472
21	17.849493552273437	25.82381419838472	33.42861764312168	22.89807460622016
22	12.670675114898932	32.56185578510553	34.58374235866316	20.183726741332382
23	11.865267056356252	24.571081165499084	48.54033733434474	15.02331444379992
24	12.513665164428184	25.15366337959038	46.55860961135157	15.77406184462987
25	11.692918656017135	34.97584891347999	36.02862433626344	17.302608094239435
26	11.28380036589175	35.660501539422604	37.322631743340324	15.73306635134532
27	11.230534112712508	29.856153228325375	44.95560215965374	13.957710499308376
28	11.241410468073713	26.514602204274684	43.22012627727455	19.023861050377047
29	13.342778100040158	32.354926152335906	37.129088394092186	17.173207353531748
30	23.055921199411003	28.98604479942885	31.894493775378162	16.063540225781985
31	22.366806478961223	28.200158404355	34.356454419704605	15.07658069697916
32	20.27352639328901	30.264992637544065	32.76878541787515	16.692695551291774
33	13.510385524965418	29.23508544910981	35.36795546829682	21.88657355762795
34	15.035585203694616	27.004038195528977	37.42637544063183	20.534001160144573
35	24.480165989915665	26.580139217348624	32.85244968988443	16.087245102851277
36	15.958402123956986	32.489625630270844	34.80684708402124	16.745125161750927
37	15.598924635223774	33.70805631163268	32.72555887733702	17.967460175806522
38	15.740317254919459	33.40798045602606	33.09423943599125	17.757462853063227
39	19.996039891124894	26.773124804783365	31.399480165989917	21.831355138101824
40	13.015093034670475	25.55441524251484	34.52238855918968	26.908103163625007
41	16.349393155147027	28.75234259961626	30.80072285931016	24.097541385926554
42	16.335727990718844	26.96136941680425	33.14388023738343	23.559022355093482
43	16.70636071571996	29.48552050332426	35.37799518093793	18.430123600017847
44	13.247958591762973	37.71920039266431	30.079815715496856	18.953025300075858
45	17.438701976707865	33.26993440721074	31.97620588104056	17.315157735040827
46	16.395687385658828	30.87518406139842	31.22880505109098	21.50032350185177
47	16.379791173977065	28.565492392128867	36.18145107313373	18.873265360760342
48	17.168466378117888	25.619952255588775	36.82204051581813	20.389540850475214
49	17.38459908080853	32.2400272187765	31.623979295881487	18.75139440453349
50	14.627303556289323	32.42129980812994	31.96728169202624	20.984114943554506
51	15.25729552451921	27.3395319262862	36.082169470349385	21.32100307884521
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	622.0
1	2655.0
2	4688.0
3	17904.0
4	31120.0
5	17580.0
6	4040.0
7	4156.5
8	4273.0
9	5515.0
10	6757.0
11	7476.5
12	8196.0
13	8396.0
14	8596.0
15	7543.5
16	6491.0
17	5584.0
18	4677.0
19	3938.0
20	3199.0
21	2949.5
22	2700.0
23	2462.0
24	2224.0
25	2175.0
26	2331.0
27	2536.0
28	2454.0
29	2372.0
30	2587.0
31	2802.0
32	3409.0
33	4016.0
34	4736.5
35	5457.0
36	6213.5
37	6970.0
38	8533.5
39	10097.0
40	13144.5
41	16192.0
42	22488.5
43	28785.0
44	31207.0
45	33629.0
46	33068.5
47	32508.0
48	30497.5
49	28487.0
50	26305.5
51	24124.0
52	21207.5
53	18291.0
54	15823.5
55	13356.0
56	12040.0
57	10724.0
58	9475.0
59	8226.0
60	6898.0
61	5570.0
62	4699.5
63	3829.0
64	3316.5
65	2804.0
66	2491.0
67	2178.0
68	1841.5
69	1505.0
70	1328.5
71	1152.0
72	1283.0
73	1414.0
74	1167.5
75	606.0
76	291.0
77	309.0
78	327.0
79	245.5
80	164.0
81	112.5
82	61.0
83	63.5
84	66.0
85	36.0
86	6.0
87	5.0
88	4.0
89	3.5
90	3.0
91	1.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	358576.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.05556642167848
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.25961812953946	27.784908078969423
2	8.996330003118462	6.3074288795131634
3	3.3977624755493943	3.573314644401254
4	1.8778023803227313	2.633097042807578
5	1.1351832281657734	1.9897245527870335
6	0.7887291773143126	1.6589608838434622
7	0.5259955582869486	1.2907350561725186
8	0.4118395142296234	1.1549813956918689
9	0.28652876456842963	0.9039985324245055
>10	2.4997356670804405	20.230920731542927
>50	0.6712252535353671	16.104324787289816
>100	0.14605733816524635	7.61303492871933
>500	7.981275309576303E-4	0.14213236838623372
>1k	0.0015962550619152607	1.1163546257107728
>5k	0.0	0.0
>10k+	7.981275309576303E-4	7.496083491740106
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	26792	7.471777252242203	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2817	0.78560751416715	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1173	0.32712730355628933	No Hit
CTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	508	0.14167150060238276	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	487	0.13581500156173307	No Hit
CCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC	413	0.11517781446611039	Illumina PCR Primer Index 2 (95% over 24bp)
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	409	0.11406229083931999	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	390	0.10876355361206551	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.10485922091829905	0.0
2	0.0	0.0	0.0	0.3628240596135826	0.0
3	0.0	0.0	0.0	0.5915064031056178	0.0
4	0.0	0.0	0.0	0.7471219490428808	0.0
5	0.0	0.0	0.0	1.6493016822096291	0.0
6	0.0	0.0	0.0	1.8896970237829638	0.0
7	0.0	0.0	0.0	2.636261211012449	0.0
8	0.0	0.0	0.0	3.501349783588416	0.0
9	0.0	0.0	0.0	4.1461224398732766	0.0
10	0.0	0.0	0.0	4.5750412743741915	0.0
11	0.0	0.0	0.0	5.097942974432199	0.0
12	0.0	0.0	0.0	5.533833831600553	0.0
13	0.0	0.0	0.0	5.707855517379858	0.0
14	0.0	0.0	0.0	5.859845611530052	0.0
15	0.0	0.0	0.0	6.384699477934943	0.0
16	0.0	0.0	0.0	6.970070501093213	0.0
17	0.0	0.0	0.0	7.885078755968052	0.0
18	0.0	0.0	0.0	8.211369416804247	0.0
19	0.0	0.0	0.0	8.64056713221186	0.0
20	0.0	0.0	0.0	9.005622239079024	0.0
21	0.0	0.0	0.0	9.367051894159118	0.0
22	0.0	0.0	0.0	9.720394002944982	0.0
23	0.0	0.0	0.0	10.006246932310026	0.0
24	0.0011155236267904154	0.0	0.0	10.236881442148945	0.0
25	0.0013944045334880193	0.0	0.0	10.491499709963858	0.0
26	0.0013944045334880193	0.0	0.0	10.727990718843426	0.0
27	0.003346570880371246	0.0	0.0	10.958625228682344	0.0
28	0.003346570880371246	0.0	0.0	11.203203783856143	0.0
29	0.003346570880371246	0.0	0.0	11.509136138503413	0.0
30	0.003346570880371246	0.0	0.0	11.816462897684174	0.0
31	0.003346570880371246	0.0	0.0	12.049607335683369	0.0
32	0.003346570880371246	0.0	0.0	12.286098344562937	0.0
33	0.003346570880371246	0.0	0.0	12.536812279684083	0.0
34	0.003346570880371246	0.0	0.0	12.864497345053769	0.0
35	0.003346570880371246	0.0	0.0	13.131386372763375	0.0
36	0.003346570880371246	0.0	0.0	13.37875373700415	0.0
37	0.003346570880371246	0.0	0.0	13.650383740127616	0.0
38	0.003904332693766454	0.0	0.0	13.928706885011824	0.0
39	0.003904332693766454	0.0	0.0	14.220137432510821	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTTAAC	25	3.885418E-5	45.000004	34
GTACCGG	30	2.1613087E-6	45.000004	1
TCGCAGG	30	2.1613087E-6	45.000004	1
CGTATTT	25	3.885418E-5	45.000004	1
TCCGCGG	25	3.885418E-5	45.000004	1
TACCAGG	25	3.885418E-5	45.000004	1
CCGATTC	30	2.1613087E-6	45.000004	11
CGCGGGT	25	3.885418E-5	45.000004	3
CAGTTTC	25	3.885418E-5	45.000004	9
ATTACGG	25	3.885418E-5	45.000004	1
GCGCCTT	25	3.885418E-5	45.000004	31
ATTCGGC	25	3.885418E-5	45.000004	16
TGTATCA	25	3.885418E-5	45.000004	24
GGATAAT	30	2.1613087E-6	45.000004	7
ACTTAGG	30	2.1613087E-6	45.000004	1
GCGTTCG	25	3.885418E-5	45.000004	44
CGCCTTA	25	3.885418E-5	45.000004	32
GGCTTAT	25	3.885418E-5	45.000004	7
GAAAGGT	25	3.885418E-5	45.000004	8
ACGATGG	30	2.1613087E-6	45.000004	1
>>END_MODULE