##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043407_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2592779
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.614225894301057	31.0	31.0	33.0	30.0	34.0
2	30.648015507685	31.0	31.0	34.0	26.0	34.0
3	31.580754086638315	31.0	31.0	34.0	28.0	34.0
4	35.203062042696274	37.0	35.0	37.0	33.0	37.0
5	35.526721328736464	37.0	35.0	37.0	33.0	37.0
6	35.842466326671115	37.0	35.0	37.0	35.0	37.0
7	33.76943657751008	37.0	35.0	37.0	32.0	37.0
8	34.82085515194315	37.0	35.0	37.0	32.0	37.0
9	36.893417063313144	39.0	37.0	39.0	32.0	39.0
10	36.880878779101494	39.0	37.0	39.0	33.0	39.0
11	36.59183408998607	39.0	35.0	39.0	33.0	39.0
12	36.49175614273334	39.0	35.0	39.0	33.0	39.0
13	36.53836289170808	39.0	35.0	39.0	33.0	39.0
14	37.85368016325341	40.0	36.0	41.0	33.0	41.0
15	37.97137550095862	40.0	36.0	41.0	33.0	41.0
16	37.84587155326389	40.0	36.0	41.0	33.0	41.0
17	37.812894967137574	40.0	36.0	41.0	33.0	41.0
18	37.52154657223003	39.0	36.0	41.0	33.0	41.0
19	37.2141038630751	38.0	35.0	41.0	33.0	41.0
20	36.85365779343322	38.0	35.0	40.0	33.0	41.0
21	36.75485724005016	38.0	35.0	40.0	32.0	41.0
22	36.68753295209503	38.0	35.0	40.0	32.0	41.0
23	36.621644575183616	37.0	35.0	40.0	32.0	41.0
24	36.47972619340098	37.0	35.0	40.0	32.0	41.0
25	36.38077136539597	37.0	35.0	40.0	32.0	41.0
26	36.117779803060735	36.0	35.0	40.0	31.0	41.0
27	36.021611946101075	36.0	35.0	40.0	31.0	41.0
28	35.88384123752931	36.0	35.0	40.0	31.0	41.0
29	35.78406296872969	36.0	35.0	40.0	31.0	41.0
30	35.56845338534445	36.0	35.0	40.0	30.0	41.0
31	35.159937271938716	36.0	34.0	40.0	28.0	41.0
32	34.948455306063494	36.0	34.0	40.0	26.0	41.0
33	34.64588728927533	36.0	34.0	40.0	24.0	41.0
34	34.42169733710432	36.0	34.0	40.0	23.0	41.0
35	34.173913395626855	36.0	34.0	40.0	21.0	41.0
36	34.004566528809434	35.0	34.0	40.0	21.0	41.0
37	33.891829191766824	35.0	33.0	40.0	20.0	41.0
38	33.78434914815339	35.0	33.0	40.0	20.0	41.0
39	33.63247118246484	35.0	33.0	40.0	18.0	41.0
40	33.56043650461532	35.0	33.0	40.0	18.0	41.0
41	33.4544641097448	35.0	33.0	40.0	18.0	41.0
42	33.33615591610392	35.0	33.0	40.0	18.0	41.0
43	33.146859026550274	35.0	33.0	40.0	18.0	41.0
44	32.92302544875595	35.0	33.0	40.0	16.0	41.0
45	32.718642815295865	35.0	33.0	40.0	15.0	41.0
46	32.70857755327392	35.0	33.0	39.0	15.0	41.0
47	32.67652545781958	35.0	33.0	39.0	15.0	41.0
48	32.59422727505893	35.0	33.0	39.0	15.0	41.0
49	32.5077497927899	35.0	32.0	39.0	15.0	41.0
50	32.41922315785495	35.0	32.0	39.0	15.0	41.0
51	31.99055762176414	35.0	31.0	38.0	12.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	3.0
11	6.0
12	14.0
13	36.0
14	65.0
15	131.0
16	300.0
17	665.0
18	1444.0
19	2743.0
20	4776.0
21	7744.0
22	12261.0
23	19822.0
24	31603.0
25	48770.0
26	62625.0
27	68201.0
28	67925.0
29	66844.0
30	70792.0
31	81201.0
32	98562.0
33	131083.0
34	240681.0
35	304751.0
36	263298.0
37	246373.0
38	317290.0
39	442505.0
40	263.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.697223326785664	16.718817916991767	22.75616240335177	33.8277963528708
2	34.971819811869814	19.63098281804967	26.86295283940513	18.534244530675387
3	23.341326044371698	20.159759084750377	38.57301374316901	17.92590112770892
4	22.013831491230064	23.098420652126542	35.60901256913914	19.278735287504254
5	19.613279805182007	30.772657445929635	33.99815410414848	15.61590864473987
6	76.18065403954598	2.050309725587873	20.42071460776256	1.3483216271035827
7	72.47787026969904	7.2539541549819715	18.889577553659606	1.3785980216593856
8	72.15998741119085	3.409430576227283	21.829126200111926	2.6014558124699407
9	41.14492596553737	19.269401672876864	27.604435241106163	11.981237120479609
10	30.225329655940598	18.406852261608105	35.349754066968295	16.018064015483002
11	27.781658213060194	16.4624906326378	40.11872974904533	15.637121405256677
12	19.337089663253213	23.90705108302713	39.75317603235756	17.002683221362098
13	15.257952953182667	26.515719234072783	41.51202242844454	16.714305384300012
14	15.034486163302002	19.724974631466853	47.986002663551346	17.254536541679798
15	18.31552168541939	16.36529762081535	46.83268415858043	18.486496535184834
16	18.554338800183125	17.36642421124207	39.466418078825846	24.61281890974896
17	19.49788238797059	19.73704662063369	42.08314707886789	18.681923912527832
18	22.166756210228485	22.479316594279727	37.03408582065806	18.31984137483372
19	23.70337001341032	21.03503615232922	36.87529866602591	18.386295168234547
20	21.008500917355473	23.794392040355156	38.76766974740231	16.42943729488707
21	19.965720179004844	20.0838559707557	38.059394958073945	21.891028892165508
22	18.976357028501077	22.847454410885	37.70433191567812	20.4718566449358
23	19.043659332322576	20.301383187691663	43.787148846855054	16.867808633130707
24	20.118683466658744	19.794243936718093	40.806871700210465	19.28020089641269
25	16.964770233020246	23.673055050199032	38.27757784215315	21.08459687462757
26	15.995269940091308	24.207346634634114	39.315961753778474	20.481421671496104
27	16.67388543335163	23.268469854160344	41.82882536459914	18.228819347888887
28	17.68099016537854	21.975455679022392	40.190660291525035	20.15289386407403
29	18.004156929688182	23.18751424629712	41.06582165313743	17.74250717087727
30	23.975317603235755	22.3101159026666	36.39195627548665	17.322610218610997
31	24.177918750498982	22.084566405389737	36.28558392365875	17.451930920452533
32	23.79238647026993	22.119162489359873	35.05184977200139	19.036601268368806
33	18.779464042249646	23.085114465984184	37.287134769295804	20.84828672247037
34	19.31444986248346	23.695000615170056	36.9095090634412	20.081040458905292
35	24.385263842386877	24.907522006310604	33.339517174429446	17.367696976873077
36	20.833977751285396	26.565781348892443	33.90165532812476	18.698585571697397
37	20.588295415845316	28.152225854961028	33.05152502392221	18.20795370527145
38	20.288539825415125	26.54321868543366	32.89134939769259	20.276892091458624
39	21.578854194669116	22.852931159963884	35.19551801368338	20.37269663168361
40	18.000222926828705	23.101390438598894	35.98093011398195	22.917456520590456
41	18.798632664025742	25.340493732786328	32.469369738030124	23.39150386515781
42	18.46150404643049	22.852082649543213	33.92140247973314	24.765010824293164
43	19.187636123248453	23.26503724382217	35.93379921697915	21.61352741595022
44	19.58659029558632	27.490542001458667	32.739234620459364	20.183633082495657
45	21.243384029259722	25.948682861130855	33.739551269120895	19.068381840488527
46	19.76223195266546	25.325104839247775	33.36366115276312	21.54900205532365
47	18.8202311110974	25.53067577298335	35.79094091706235	19.858152198856903
48	20.686066957500042	23.912450694795044	35.627911210326836	19.77357113737808
49	19.589752925336096	25.28352782863483	34.86294049743538	20.26377874859369
50	18.103239805629403	25.346394737075546	35.598483326191705	20.951882131103343
51	17.99844876867639	22.358519565300398	36.7246880663566	22.918343599666613
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1327.0
1	14802.0
2	28277.0
3	114214.5
4	200152.0
5	119053.0
6	37954.0
7	35589.5
8	33225.0
9	35016.5
10	36808.0
11	38267.0
12	39726.0
13	38152.5
14	36579.0
15	33330.5
16	30082.0
17	27391.0
18	24700.0
19	22496.5
20	20293.0
21	19271.5
22	18250.0
23	17564.5
24	16879.0
25	17019.0
26	18298.5
27	19438.0
28	21267.5
29	23097.0
30	27226.5
31	31356.0
32	34788.0
33	38220.0
34	44808.5
35	51397.0
36	56511.0
37	61625.0
38	64428.0
39	67231.0
40	79062.0
41	90893.0
42	109695.0
43	128497.0
44	136242.5
45	143988.0
46	145393.5
47	146799.0
48	154958.5
49	163118.0
50	157005.5
51	150893.0
52	137270.5
53	123648.0
54	119497.5
55	115347.0
56	115964.5
57	116582.0
58	115251.0
59	113920.0
60	110043.0
61	106166.0
62	101980.0
63	97794.0
64	89164.0
65	80534.0
66	67824.0
67	55114.0
68	46171.0
69	37228.0
70	32553.0
71	27878.0
72	26425.5
73	24973.0
74	21185.5
75	13248.5
76	9099.0
77	6839.5
78	4580.0
79	3527.5
80	2475.0
81	1826.5
82	1178.0
83	845.0
84	512.0
85	371.0
86	230.0
87	156.0
88	82.0
89	60.5
90	39.0
91	31.5
92	24.0
93	15.0
94	6.0
95	7.0
96	8.0
97	4.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2592779.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.2364726370345
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.08225681932247	43.97615603330188
2	11.766255694084052	12.763204099850823
3	3.4113753800600928	5.550629023658472
4	1.2353331505608427	2.680004504720591
5	0.5992161786985188	1.6249685939825298
6	0.3343914856089971	1.0881728795573813
7	0.24387373621986025	0.9258795854965874
8	0.15878827764034992	0.6889692860258142
9	0.13231660476775087	0.6458747322520286
>10	0.8333689362259412	8.699524226873525
>50	0.11555982728831894	4.52365169647319
>100	0.08418192757463426	7.477213756631
>500	0.0016143714965694885	0.60526329914516
>1k	0.0011007078385698646	1.0635090662647535
>5k	1.467610451426486E-4	0.47786667759302803
>10k+	2.2014156771397292E-4	7.209112538173225
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	151906	5.8588101801194785	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15402	0.5940344317814977	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	13830	0.5334045053589218	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6518	0.25139049645187656	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	5489	0.21170334995770948	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	4205	0.1621811963148421	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.052839058014585896	0.0
2	0.0	0.0	0.0	0.17691442271015	0.0
3	0.0	0.0	0.0	0.2794299089895437	0.0
4	0.0	0.0	0.0	0.3549473364293679	0.0
5	0.0	0.0	0.0	0.739168282371926	0.0
6	0.0	0.0	0.0	0.855799896558866	0.0
7	0.0	0.0	0.0	1.1875674710416892	0.0
8	0.0	0.0	0.0	1.57861506900511	0.0
9	0.0	0.0	0.0	1.9037874033999813	0.0
10	0.0	0.0	0.0	2.1301468424420285	0.0
11	0.0	0.0	0.0	2.3720494496445705	3.856865548509919E-5
12	0.0	0.0	0.0	2.563504255472603	3.856865548509919E-5
13	0.0	0.0	0.0	2.652250731743816	3.856865548509919E-5
14	0.0	0.0	0.0	2.7238341563241604	3.856865548509919E-5
15	0.0	0.0	0.0	2.939085822586499	3.856865548509919E-5
16	0.0	0.0	0.0	3.1930218503003918	3.856865548509919E-5
17	0.0	0.0	0.0	3.5206240099908244	3.856865548509919E-5
18	0.0	0.0	0.0	3.6917917030336946	3.856865548509919E-5
19	0.0	0.0	0.0	3.8895717683612836	3.856865548509919E-5
20	0.0	0.0	0.0	4.10420633613586	3.856865548509919E-5
21	3.856865548509919E-5	0.0	0.0	4.328367361815257	3.856865548509919E-5
22	7.713731097019838E-5	0.0	0.0	4.558660803716784	3.856865548509919E-5
23	1.5427462194039676E-4	0.0	0.0	4.750848413999034	3.856865548509919E-5
24	6.942357987317854E-4	0.0	0.0	4.918004966871453	3.856865548509919E-5
25	6.942357987317854E-4	0.0	0.0	5.085431500332269	3.856865548509919E-5
26	6.942357987317854E-4	0.0	0.0	5.26477574833798	3.856865548509919E-5
27	6.942357987317854E-4	0.0	0.0	5.449288196178695	3.856865548509919E-5
28	7.328044542168847E-4	0.0	0.0	5.637117548391128	3.856865548509919E-5
29	7.328044542168847E-4	0.0	0.0	5.85595609961358	3.856865548509919E-5
30	7.328044542168847E-4	0.0	0.0	6.056590245447067	3.856865548509919E-5
31	7.328044542168847E-4	0.0	0.0	6.256453018170851	3.856865548509919E-5
32	7.328044542168847E-4	0.0	0.0	6.466575053253671	3.856865548509919E-5
33	7.328044542168847E-4	3.856865548509919E-5	0.0	6.677622736068134	3.856865548509919E-5
34	7.328044542168847E-4	3.856865548509919E-5	0.0	6.929823174285198	3.856865548509919E-5
35	7.328044542168847E-4	3.856865548509919E-5	0.0	7.14654044945597	3.856865548509919E-5
36	7.328044542168847E-4	3.856865548509919E-5	0.0	7.3802665016956706	3.856865548509919E-5
37	8.099417651870831E-4	3.856865548509919E-5	0.0	7.651211306478492	3.856865548509919E-5
38	8.099417651870831E-4	3.856865548509919E-5	0.0	7.932068255720985	3.856865548509919E-5
39	8.099417651870831E-4	3.856865548509919E-5	0.0	8.241774559266332	3.856865548509919E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	38645	0.0	44.36538	1
ATTACGG	195	0.0	42.692307	1
CGTTTTA	1915	0.0	42.650135	1
CGACGGT	545	0.0	42.11009	27
TACGGGA	530	0.0	42.0283	3
CGCACTT	4280	0.0	41.950935	34
CCGCACT	4330	0.0	41.362587	33
ACCCGCA	4340	0.0	41.319126	31
CCCGCAC	4390	0.0	41.156033	32
TCGATGG	175	0.0	41.142857	1
CACTTAC	4370	0.0	41.03547	36
AGGGTAC	790	0.0	41.012657	5
ACGGGAT	720	0.0	40.9375	4
TCACGTT	485	0.0	40.824738	45
CTTGCGG	750	0.0	40.8	1
GCACTTA	4440	0.0	40.43919	35
CTTACTG	4370	0.0	40.366135	38
ACTTACT	4395	0.0	40.341297	37
TTATGAC	4515	0.0	40.11628	26
GCGCGAC	1970	0.0	40.088833	8
>>END_MODULE