##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043406_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	731210
Sequences flagged as poor quality	0
Sequence length	51
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.48216517826616	31.0	31.0	33.0	30.0	34.0
2	30.506925507036282	31.0	31.0	34.0	26.0	34.0
3	31.402495863021567	31.0	31.0	34.0	28.0	34.0
4	35.02313699210897	37.0	35.0	37.0	32.0	37.0
5	35.34490912323409	37.0	35.0	37.0	33.0	37.0
6	35.80172453877819	37.0	35.0	37.0	35.0	37.0
7	33.73081467704216	37.0	35.0	37.0	32.0	37.0
8	34.75712449227992	37.0	35.0	37.0	31.0	37.0
9	36.74362768561699	39.0	37.0	39.0	32.0	39.0
10	36.66535331847212	39.0	35.0	39.0	32.0	39.0
11	36.31484662408884	38.0	35.0	39.0	32.0	39.0
12	36.18242638913581	39.0	35.0	39.0	32.0	39.0
13	36.25518660849824	39.0	35.0	39.0	32.0	39.0
14	37.63186635850166	40.0	35.0	41.0	32.0	41.0
15	37.81793875904323	40.0	35.0	41.0	33.0	41.0
16	37.79939825768247	40.0	35.0	41.0	33.0	41.0
17	37.74936064878763	40.0	36.0	41.0	33.0	41.0
18	37.44533307804871	39.0	36.0	41.0	33.0	41.0
19	37.12482597338658	38.0	35.0	41.0	33.0	41.0
20	36.76981715239124	38.0	35.0	40.0	32.0	41.0
21	36.66319525170608	38.0	35.0	40.0	32.0	41.0
22	36.55684823785233	37.0	35.0	40.0	32.0	41.0
23	36.44816947251815	37.0	35.0	40.0	32.0	41.0
24	36.2418457077994	37.0	35.0	40.0	32.0	41.0
25	36.09886626276993	36.0	35.0	40.0	31.0	41.0
26	35.79535564338562	35.0	35.0	40.0	31.0	41.0
27	35.666856306669764	36.0	35.0	40.0	31.0	41.0
28	35.56656911147277	35.0	35.0	40.0	30.0	41.0
29	35.496587847540376	35.0	35.0	40.0	30.0	41.0
30	35.293726836339765	35.0	35.0	40.0	30.0	41.0
31	34.77397190957454	35.0	34.0	40.0	27.0	41.0
32	34.60981934054512	35.0	34.0	40.0	25.0	41.0
33	34.44667879268609	35.0	34.0	40.0	24.0	41.0
34	34.27751261607472	35.0	34.0	40.0	23.0	41.0
35	34.03876861640295	35.0	33.0	40.0	22.0	41.0
36	33.86540391953064	35.0	33.0	40.0	21.0	41.0
37	33.78697638161404	35.0	33.0	40.0	21.0	41.0
38	33.69981537451621	35.0	33.0	40.0	21.0	41.0
39	33.5736696708196	35.0	33.0	40.0	20.0	41.0
40	33.516628601906426	35.0	33.0	40.0	20.0	41.0
41	33.455910066875454	35.0	33.0	40.0	20.0	41.0
42	33.34308474993504	35.0	33.0	40.0	18.0	41.0
43	33.18045158025738	35.0	33.0	40.0	18.0	41.0
44	32.9743821884274	35.0	33.0	40.0	18.0	41.0
45	32.7978337276569	35.0	33.0	39.0	18.0	41.0
46	32.80563586384213	35.0	33.0	39.0	18.0	41.0
47	32.79438875288905	35.0	33.0	39.0	18.0	41.0
48	32.737037239643875	35.0	33.0	39.0	18.0	41.0
49	32.62984231616088	35.0	33.0	39.0	17.0	41.0
50	32.521263385347574	35.0	33.0	39.0	15.0	41.0
51	32.12469605174984	35.0	31.0	38.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	4.0
13	9.0
14	13.0
15	39.0
16	109.0
17	206.0
18	512.0
19	911.0
20	1508.0
21	2428.0
22	3720.0
23	5935.0
24	9161.0
25	13550.0
26	16766.0
27	18363.0
28	18643.0
29	18930.0
30	21005.0
31	23959.0
32	29455.0
33	38682.0
34	64586.0
35	95319.0
36	90896.0
37	64608.0
38	77481.0
39	114370.0
40	40.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.56284788227732	17.812940195019213	25.23720955676208	32.387002365941385
2	33.36401307421944	19.59710616649116	28.951600771324244	18.087279987965154
3	20.361182150134706	20.218678628574555	41.17804734617962	18.242091875111115
4	20.941863486549693	24.376718042696353	37.77628861749703	16.90512985325693
5	19.313056440694194	29.955279605038225	34.83787147331136	15.893792480956224
6	76.21558786121633	2.0682156972689105	20.169992204701796	1.546204236812954
7	72.70141272685001	7.296262359650442	18.449966493893683	1.5523584196058589
8	71.8566485688106	3.9691743821884278	21.643850603793712	2.530326445207259
9	41.468388014387116	20.92504205358242	26.851246563914604	10.755323368115862
10	30.96771105427989	24.013894777150202	35.17648828653875	9.841905882031154
11	28.27546122181042	21.59311278565665	38.2005169513546	11.930909041178321
12	19.11420795667455	31.066588257819234	35.76838391159858	14.050819873907633
13	13.062047838514243	33.51458541321918	37.03573528808414	16.387631460182437
14	11.954978733879459	26.20259569754243	46.090726330329176	15.751699238248928
15	13.670354617688488	24.29548282983001	45.61767481298122	16.41648773950028
16	15.9657280398244	24.819545684550263	36.03137265628205	23.18335361934328
17	16.695477359445306	25.49527495521123	41.7335649129525	16.075682772390966
18	20.834780705953147	27.540925315572817	35.88791181739856	15.736382161075477
19	21.821774866317472	27.260157820598735	35.81228374885464	15.105783564229155
20	16.585796146113978	30.896049014646955	36.94697829624868	15.571176542990386
21	17.391857332366897	27.71132779912747	35.45657198342474	19.440242885080895
22	13.620984395727628	31.447874071744096	35.09128704476142	19.839854487766853
23	13.241476456831828	27.728969789800466	44.390804283311226	14.638749470056482
24	13.973003651481791	27.809521204578715	42.763364833631925	15.454110310307573
25	13.790019283106083	32.83147112320674	36.68139111883043	16.697118474856744
26	13.472600210609812	34.49993845817207	36.40623076817877	15.621230563039346
27	13.74967519590815	31.286497722952365	40.81905334992683	14.144773731212648
28	12.872635768110394	27.953665841550308	40.36432762133997	18.80937076899933
29	14.389846965987882	32.90764623022114	37.598774633826125	15.103732169964854
30	19.779680256014004	31.86704229974973	33.6833467813624	14.669930662873867
31	19.553343088852724	29.61351732060557	35.5209857633238	15.3121538272179
32	18.387877627494152	32.66681254359213	33.24585276459567	15.699457064318048
33	15.06065289041452	32.25995268117231	33.61018038593564	19.069214042477537
34	15.994447559524625	30.12116902121142	35.528507542292914	18.355875876971048
35	21.346124916234736	30.644548077843574	33.104033041123614	14.905293964798075
36	15.59141696639816	34.23777027119432	34.104429644014715	16.066383118392803
37	15.679490160145512	33.52785109612834	33.593358952968366	17.199299790757784
38	15.164726959423422	34.64914320099561	33.47752355684413	16.708606282736834
39	18.562793178430272	30.707867780801685	32.462083396014826	18.267255644753217
40	14.029758892794137	30.05005402004896	34.892575320359406	21.0276117667975
41	16.448899768876245	30.248765744450978	32.793315189890734	20.509019296782046
42	15.871774182519385	30.917657034230935	33.79261771584087	19.417951067408815
43	16.146934533171045	31.624430738091657	33.60484676084845	18.62378796788884
44	13.656678655926477	37.86190013812721	31.29538709809767	17.186034107848634
45	17.29434772500376	33.922539352579975	32.25885860423134	16.524254318184926
46	15.694943996936583	33.422819709796094	31.410401936516188	19.47183435675114
47	14.909259993709057	31.56466678519167	35.35126707785725	18.174806143242023
48	16.551195962856088	29.637997292159575	35.5617401293746	18.249066615609742
49	16.92906278634045	33.36004704530846	32.55767836873128	17.153211799619807
50	14.817357530668342	32.33804242283338	33.137812666675785	19.706787379822487
51	14.430191053185816	30.61186252923237	35.54163646558444	19.416309951997373
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	702.0
1	4806.0
2	8910.0
3	36264.5
4	63619.0
5	37216.5
6	10814.0
7	11129.5
8	11445.0
9	13768.5
10	16092.0
11	18260.5
12	20429.0
13	20008.5
14	19588.0
15	17373.0
16	15158.0
17	13022.0
18	10886.0
19	9485.5
20	8085.0
21	7347.0
22	6609.0
23	6027.0
24	5445.0
25	5461.5
26	5901.0
27	6324.0
28	6558.5
29	6793.0
30	7781.5
31	8770.0
32	9672.0
33	10574.0
34	12356.5
35	14139.0
36	16408.0
37	18677.0
38	21174.0
39	23671.0
40	28032.0
41	32393.0
42	39507.0
43	46621.0
44	49152.5
45	51684.0
46	50344.5
47	49005.0
48	49114.5
49	49224.0
50	49737.0
51	50250.0
52	47460.5
53	44671.0
54	39784.0
55	34897.0
56	29797.0
57	24697.0
58	21212.0
59	17727.0
60	14862.0
61	11997.0
62	10202.5
63	8408.0
64	7058.5
65	5709.0
66	4681.0
67	3653.0
68	2948.0
69	2243.0
70	2026.5
71	1810.0
72	1679.0
73	1548.0
74	1206.0
75	716.0
76	568.0
77	514.5
78	461.0
79	382.0
80	303.0
81	225.0
82	147.0
83	99.5
84	52.0
85	44.0
86	36.0
87	23.5
88	11.0
89	8.0
90	5.0
91	7.0
92	9.0
93	8.0
94	7.0
95	4.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	731210.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.82257875822716
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.91574499364414	28.26965490298988
2	8.964652066353885	6.422739093741319
3	3.3686954395916784	3.6202607309176065
4	1.8384245060931121	2.6342842664230153
5	1.178178941005693	2.1102703952730555
6	0.805789493697803	1.731927456032492
7	0.59862989128206	1.5011126499236391
8	0.44229401527945783	1.2675289757312724
9	0.33273937807608883	1.0727624319384794
>10	2.889341949135251	22.38196641867462
>50	0.5351721879141577	13.08908103984148
>100	0.12678248871047862	6.869673560945897
>500	0.002369766144121096	0.5919741767722049
>1k	3.949610240201826E-4	0.3023539234613293
>5k	3.949610240201826E-4	0.7348742529050746
>10k+	3.949610240201826E-4	7.3995357244286675
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	52299	7.152391241914087	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5194	0.7103294539188468	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2137	0.2922553028541732	No Hit
CTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	976	0.1334773867972265	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.08287632827778614	0.0
2	0.0	0.0	0.0	0.2892465912665308	0.0
3	0.0	0.0	0.0	0.47838514243514174	0.0
4	0.0	0.0	0.0	0.6029731540870612	0.0
5	0.0	0.0	0.0	1.3111144541239863	0.0
6	0.0	0.0	0.0	1.5608375158983054	0.0
7	0.0	0.0	0.0	2.2098986611233435	0.0
8	0.0	0.0	0.0	2.987377087293664	0.0
9	0.0	0.0	0.0	3.6146934533171047	0.0
10	0.0	0.0	0.0	3.9984409403591306	0.0
11	0.0	0.0	0.0	4.434020322479179	0.0
12	0.0	0.0	0.0	4.783304385880937	0.0
13	0.0	0.0	0.0	4.9341502441159175	0.0
14	0.0	0.0	0.0	5.066396794354563	0.0
15	0.0	0.0	0.0	5.459990973865237	0.0
16	0.0	0.0	0.0	5.929213221919832	0.0
17	0.0	0.0	0.0	6.524801356655407	0.0
18	0.0	0.0	0.0	6.825398996184407	0.0
19	1.3675961762010913E-4	0.0	0.0	7.189589857906757	0.0
20	1.3675961762010913E-4	0.0	0.0	7.577850412330247	0.0
21	1.3675961762010913E-4	0.0	0.0	7.966110966753737	0.0
22	1.3675961762010913E-4	0.0	0.0	8.353277444236266	0.0
23	1.3675961762010913E-4	0.0	0.0	8.675209584114002	0.0
24	0.001094076940960873	0.0	0.0	8.96090042532241	0.0
25	0.0020513942643016372	0.0	0.0	9.242214958766976	0.0
26	0.0020513942643016372	0.0	0.0	9.57440406996622	0.0
27	0.0020513942643016372	0.0	0.0	9.851889334117422	0.0
28	0.0020513942643016372	0.0	0.0	10.151392896705461	0.0
29	0.0020513942643016372	0.0	0.0	10.516267556515912	0.0
30	0.0020513942643016372	0.0	0.0	10.831361715512644	0.0
31	0.0020513942643016372	0.0	0.0	11.145361797568414	0.0
32	0.0020513942643016372	0.0	0.0	11.447463792891234	0.0
33	0.0020513942643016372	0.0	0.0	11.768712134680872	0.0
34	0.0020513942643016372	0.0	0.0	12.163126871897266	0.0
35	0.0020513942643016372	0.0	0.0	12.473707963512535	0.0
36	0.0020513942643016372	0.0	0.0	12.803435401594617	0.0
37	0.0020513942643016372	0.0	0.0	13.139590541704845	0.0
38	0.0020513942643016372	0.0	0.0	13.490652480135665	0.0
39	0.0020513942643016372	0.0	0.0	13.837064591567401	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCGAG	20	7.0316E-4	45.000004	2
CTAGATT	20	7.0316E-4	45.000004	32
CGGGTGT	110	0.0	45.000004	5
GTGCAAC	20	7.0316E-4	45.000004	41
GTCGACT	35	1.2111195E-7	45.000004	33
CGGCCTA	20	7.0316E-4	45.000004	19
CGGTTCA	20	7.0316E-4	45.000004	40
GATCCGG	40	6.8084773E-9	45.000004	1
GAATCGG	20	7.0316E-4	45.000004	1
CGCGCGT	30	2.1644137E-6	45.000004	45
TTCGTCG	35	1.2111195E-7	45.000004	30
TTCCGTA	20	7.0316E-4	45.000004	45
TTACTCG	20	7.0316E-4	45.000004	14
CGACATC	55	1.8189894E-12	45.000004	10
TTATCGG	30	2.1644137E-6	45.000004	1
TCGGCTT	20	7.0316E-4	45.000004	14
GCACCGG	30	2.1644137E-6	45.000004	1
GTCAGGT	20	7.0316E-4	45.000004	38
CTCGGTA	20	7.0316E-4	45.000004	20
CTACGTC	30	2.1644137E-6	45.000004	13
>>END_MODULE