FastQCFastQC Report
Thu 26 May 2016
SRR1043405_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043405_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2289789
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT798613.4877012685448308No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC174800.7633891157656885No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90130.3936170537984067No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC71400.31181912394548145No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA71280.3112950581909512No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC60560.26447851745291817No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC38720.16909855012841793No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35810.15638995558106009No Hit
TTACTGGGAATTCCTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCAT29270.12782837195916305No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC28780.1256884367948313No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTAT253.8920236E-545.022
TGCGATA207.0347567E-445.045
ACGTATC253.8920236E-545.023
CGTTTTT210700.043.622451
ACGTAGG1200.043.1249961
CGACGGT6850.043.02919827
TACGCGG2350.042.127661
CACGACG7150.041.5384625
GACGGTC7150.041.22377828
CGGTCTA7100.041.19718630
TAGTCAG10550.040.73459633
CATATGC25600.040.6054732
ACGGGTA3000.040.5000044
TAGCATA25650.040.43859529
GCATAGG4900.040.4081651
CTTACTG58000.040.34482638
TTACGGG9100.040.3021972
GCGCGAC31100.040.1527338
GGTACCT26500.040.0754747
ACTTACT58800.040.0255137