##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043405_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2289789
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.495212440971635	31.0	31.0	33.0	30.0	34.0
2	30.51248128102633	31.0	31.0	34.0	26.0	34.0
3	31.50037099488206	31.0	31.0	34.0	28.0	34.0
4	35.11597007409853	37.0	35.0	37.0	32.0	37.0
5	35.429473632723365	37.0	35.0	37.0	33.0	37.0
6	35.74246229674437	37.0	35.0	37.0	35.0	37.0
7	33.65150675455249	37.0	35.0	37.0	31.0	37.0
8	34.67899968075661	37.0	35.0	37.0	31.0	37.0
9	36.68052165505206	39.0	37.0	39.0	32.0	39.0
10	36.676764977035	39.0	35.0	39.0	32.0	39.0
11	36.53954359986881	39.0	35.0	39.0	32.0	39.0
12	36.520251429280165	39.0	35.0	39.0	32.0	39.0
13	36.50046183294618	39.0	35.0	39.0	32.0	39.0
14	37.811707978333374	40.0	36.0	41.0	33.0	41.0
15	37.9153825963877	40.0	36.0	41.0	33.0	41.0
16	37.777024869977105	40.0	36.0	41.0	33.0	41.0
17	37.754786576405074	40.0	36.0	41.0	33.0	41.0
18	37.53664944673942	39.0	36.0	41.0	33.0	41.0
19	37.31750742098944	39.0	35.0	41.0	32.0	41.0
20	37.073201941314245	39.0	35.0	41.0	32.0	41.0
21	36.99333169999507	38.0	35.0	41.0	32.0	41.0
22	36.904107321678985	38.0	35.0	41.0	32.0	41.0
23	36.84170681228707	38.0	35.0	40.0	32.0	41.0
24	36.70308093889874	38.0	35.0	40.0	32.0	41.0
25	36.58881800899559	38.0	35.0	40.0	31.0	41.0
26	36.304924602223174	38.0	35.0	40.0	31.0	41.0
27	36.166210074378036	38.0	35.0	40.0	31.0	41.0
28	35.98439332183009	38.0	35.0	40.0	30.0	41.0
29	35.92808638699898	37.0	35.0	40.0	30.0	41.0
30	35.754034978768786	37.0	35.0	40.0	30.0	41.0
31	35.53541352500165	38.0	34.0	40.0	29.0	41.0
32	35.447091849947746	38.0	34.0	40.0	27.0	41.0
33	35.23394644659399	37.0	34.0	40.0	26.0	41.0
34	35.11029793574866	37.0	34.0	40.0	26.0	41.0
35	34.92830256412272	37.0	34.0	40.0	25.0	41.0
36	34.77525745821995	37.0	34.0	40.0	24.0	41.0
37	34.64612023203885	37.0	34.0	40.0	23.0	41.0
38	34.51687469893514	37.0	34.0	40.0	23.0	41.0
39	34.37532235502922	36.0	34.0	40.0	23.0	41.0
40	34.25436535855487	36.0	33.0	40.0	23.0	41.0
41	34.142720573817066	36.0	33.0	40.0	23.0	41.0
42	34.03337032364117	36.0	33.0	40.0	22.0	41.0
43	33.85332491334354	35.0	33.0	40.0	22.0	41.0
44	33.635322293888215	35.0	33.0	40.0	21.0	41.0
45	33.438202384586525	35.0	33.0	40.0	20.0	41.0
46	33.43980165858077	35.0	33.0	40.0	20.0	41.0
47	33.38849955170542	35.0	33.0	39.0	21.0	41.0
48	33.2746362219401	35.0	33.0	39.0	20.0	41.0
49	33.225205466529886	35.0	33.0	39.0	20.0	41.0
50	33.14229346022712	35.0	33.0	39.0	20.0	41.0
51	32.73254740939012	35.0	32.0	38.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	6.0
11	7.0
12	17.0
13	23.0
14	58.0
15	127.0
16	289.0
17	716.0
18	1407.0
19	2458.0
20	4185.0
21	6607.0
22	9920.0
23	14884.0
24	22360.0
25	32012.0
26	43232.0
27	51343.0
28	55238.0
29	58047.0
30	63774.0
31	73735.0
32	89227.0
33	117065.0
34	199686.0
35	228123.0
36	234965.0
37	258072.0
38	321708.0
39	400270.0
40	226.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.205621566004552	16.70970556675746	23.597676467132995	29.486996400104985
2	31.504518538607705	20.782395233796652	27.297449677677726	20.415636549917917
3	27.5223175585174	21.274449305154317	32.13999193812181	19.063241198206473
4	23.88237518828154	24.371590570135503	29.680551352111483	22.065482889471475
5	21.968312364152332	33.70533267475737	27.902876640598762	16.42347832049154
6	81.75085127931001	2.393364628793308	14.375953417541965	1.4798306743547114
7	76.76362319846938	7.8198034840764805	13.705236596035705	1.7113367214184363
8	76.0594098408194	4.135970606898715	16.56253043402689	3.242089118255001
9	43.55370735032791	17.884136922659685	23.20750951288525	15.354646214127154
10	28.08437808025106	18.675825589170007	30.887300096209735	22.352496234369195
11	24.35822689339498	15.95802058617628	39.7960248739076	19.887727646521142
12	20.650898401555775	18.71495583217493	40.36144815089949	20.2726976153698
13	17.562840942986448	22.338346458996877	41.983824710486424	18.114987887530248
14	17.02549885600813	18.345445803084914	42.65899609090619	21.970059250000766
15	21.495823414297124	15.459415692886985	41.253495409402355	21.79126548341354
16	22.007224246426198	16.532352980995192	36.2440818782866	25.216340894292006
17	22.092734308707048	19.214128463364965	36.454931000192595	22.238206227735393
18	24.825693546435936	20.9781774652599	33.260357177014996	20.93577181128916
19	24.420940095353764	20.571327751159604	32.23205282233428	22.775679331152347
20	23.95198858934164	21.860267474426685	36.12738990361121	18.060354032620474
21	22.748253223331933	17.828367591948428	35.08410600278017	24.339273181939472
22	22.10125037721816	20.680027723078414	34.36395231176322	22.8547695879402
23	23.110513676150944	20.505775859697117	36.05061427057253	20.333096193579408
24	26.13480980125243	18.24006491427813	33.84652472345705	21.77860056101239
25	21.08076333670919	20.947126569303983	32.97260140563169	24.999508688355128
26	20.443018985592122	20.761345259323022	35.20927037382047	23.586365381264386
27	21.447522020587922	20.770298049296247	35.82945852216077	21.952721407955057
28	21.426078996798395	22.598807138998396	34.01256622335071	21.9625476408525
29	21.822316379369454	21.005952950250002	36.151802633343074	21.019928037037474
30	25.854652983309816	21.163696742363598	32.55723562302029	20.4244146513063
31	26.317097339536527	21.608977945129443	31.387346170323994	20.686578545010043
32	26.362647388034443	19.643775037787325	30.834806176464298	23.158771397713938
33	22.434468852806962	22.369047977783104	33.041123003036525	22.15536016637341
34	23.239608540350222	22.29528572283298	32.28459041422594	22.180515322590857
35	27.162546418032402	24.24533439543993	28.9743290757358	19.61779011079187
36	23.167200121932634	24.255073284044947	30.19780425183281	22.379922342189605
37	24.672622673966902	27.0248918131758	27.584637711160287	20.717847801697012
38	23.29275754228883	23.42538985033119	27.943841113744543	25.338011493635438
39	23.647855763129265	21.964425543139566	32.60256731078715	21.78515138294402
40	21.56941098066241	21.57290475235928	32.49238248589717	24.36530178108114
41	21.797204895298215	25.62511218282558	29.010445940652176	23.567236981224035
42	21.794278861502086	21.619895981682156	30.812577054042972	25.77324810277279
43	22.43128078613357	21.293708721633305	33.12833627901959	23.14667421321353
44	22.97635284299121	23.580032920063815	31.78074486339134	21.662869373553633
45	24.883340779434263	22.374157618889775	31.074085865553553	21.668415736122412
46	22.548977220171814	24.39770651356959	30.332882200062976	22.72043406619562
47	21.939226714775902	26.97370805781668	30.131684622469578	20.955380604937833
48	23.399099218312255	24.76328604950063	30.871534451427618	20.966080280759495
49	21.762441866914376	23.10453059212006	32.30874111108054	22.824286429885024
50	21.35415097198912	24.867837167529412	32.65624037848029	21.12177148200118
51	20.745579614540905	22.20623821670905	33.152312287289355	23.895869881460694
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	873.0
1	10185.0
2	19497.0
3	67114.0
4	114731.0
5	69826.0
6	24921.0
7	22943.5
8	20966.0
9	21110.0
10	21254.0
11	21358.5
12	21463.0
13	20266.0
14	19069.0
15	17892.0
16	16715.0
17	15379.5
18	14044.0
19	13358.5
20	12673.0
21	12574.0
22	12475.0
23	12218.5
24	11962.0
25	12098.5
26	13349.5
27	14464.0
28	16813.0
29	19162.0
30	21250.5
31	23339.0
32	25368.5
33	27398.0
34	31185.0
35	34972.0
36	38995.0
37	43018.0
38	47858.0
39	52698.0
40	56782.0
41	60866.0
42	71192.0
43	81518.0
44	88911.5
45	96305.0
46	106991.0
47	117677.0
48	133191.0
49	148705.0
50	145036.0
51	141367.0
52	126859.5
53	112352.0
54	115017.0
55	117682.0
56	124255.0
57	130828.0
58	133103.5
59	135379.0
60	133434.0
61	131489.0
62	124052.0
63	116615.0
64	108686.0
65	100757.0
66	86725.0
67	72693.0
68	62386.5
69	52080.0
70	45990.5
71	39901.0
72	39093.5
73	38286.0
74	32254.0
75	20236.0
76	14250.0
77	11150.5
78	8051.0
79	6156.0
80	4261.0
81	3416.0
82	2571.0
83	1877.5
84	1184.0
85	841.0
86	498.0
87	344.0
88	190.0
89	148.0
90	106.0
91	60.5
92	15.0
93	11.5
94	8.0
95	6.0
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2289789.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.384146365578975
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.04325616003796	20.591046933728144
2	9.122346935358966	4.813706734802036
3	3.0872163888567115	2.4436070719742897
4	1.527806465123426	1.6123947757637722
5	0.9540606355356741	1.2586037724805261
6	0.6650303648816389	1.0527755090754933
7	0.465865836072512	0.8604030683961985
8	0.3533113320459083	0.7457454317853535
9	0.2969399689756907	0.7051056842920594
>10	4.006652534717743	26.44132156783547
>50	1.2200629099460876	21.93817342993745
>100	0.2492636490334516	9.630698647128547
>500	0.004528878824912869	0.845304091805913
>1k	0.002612814706680501	1.2392554143177537
>5k	6.967505884481337E-4	1.3482676094878525
>10k+	3.483752942240669E-4	4.473590257189115
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	79861	3.4877012685448308	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	17480	0.7633891157656885	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9013	0.3936170537984067	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	7140	0.31181912394548145	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	7128	0.3112950581909512	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	6056	0.26447851745291817	No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	3872	0.16909855012841793	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3581	0.15638995558106009	No Hit
TTACTGGGAATTCCTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCAT	2927	0.12782837195916305	No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	2878	0.1256884367948313	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04882545946373225	0.0
2	0.0	0.0	0.0	0.1513239866206013	0.0
3	0.0	0.0	0.0	0.23128768633267083	0.0
4	0.0	0.0	0.0	0.29164259239606793	0.0
5	0.0	0.0	0.0	0.6174368031290219	0.0
6	0.0	0.0	0.0	0.7101527695346602	0.0
7	0.0	0.0	0.0	0.9998737874974506	0.0
8	0.0	0.0	0.0	1.3012115963523276	0.0
9	0.0	0.0	0.0	1.5680920818468427	0.0
10	0.0	0.0	0.0	1.7563627041618246	0.0
11	0.0	0.0	0.0	1.9755532059940895	0.0
12	0.0	0.0	0.0	2.165002976256764	0.0
13	0.0	0.0	0.0	2.256627139007131	0.0
14	0.0	0.0	0.0	2.3257601464589097	0.0
15	0.0	0.0	0.0	2.541282188009463	0.0
16	0.0	0.0	0.0	2.7914799136514326	0.0
17	0.0	0.0	0.0	3.1179292065775495	0.0
18	0.0	0.0	0.0	3.296198907410246	0.0
19	0.0	0.0	0.0	3.501807371770936	0.0
20	0.0	0.0	0.0	3.7311734836703296	0.0
21	4.367214621085174E-5	0.0	0.0	3.959273103329608	0.0
22	8.734429242170348E-5	0.0	0.0	4.194665971406099	0.0
23	8.734429242170348E-5	0.0	0.0	4.399575681427415	0.0
24	5.24065754530221E-4	0.0	0.0	4.569285641602785	0.0
25	6.114100469519244E-4	0.0	0.0	4.753363737881525	0.0
26	6.114100469519244E-4	0.0	0.0	4.95613351273851	0.0
27	6.114100469519244E-4	0.0	0.0	5.164362305871851	0.0
28	8.734429242170348E-4	0.0	0.0	5.393073335578082	0.0
29	8.734429242170348E-4	0.0	0.0	5.6139233789663585	0.0
30	8.734429242170348E-4	0.0	0.0	5.859928578572087	0.0
31	8.734429242170348E-4	0.0	0.0	6.0777652438718155	0.0
32	8.734429242170348E-4	0.0	0.0	6.34254946634821	0.0
33	8.734429242170348E-4	0.0	0.0	6.593096569159866	0.0
34	8.734429242170348E-4	0.0	0.0	6.886660735989211	0.0
35	8.734429242170348E-4	0.0	0.0	7.142492168492381	0.0
36	8.734429242170348E-4	0.0	0.0	7.4186311489835965	0.0
37	8.734429242170348E-4	0.0	0.0	7.722152565149016	0.0
38	8.734429242170348E-4	0.0	0.0	8.13048713222048	0.0
39	8.734429242170348E-4	0.0	0.0	8.514671002437343	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTAT	25	3.8920236E-5	45.0	22
TGCGATA	20	7.0347567E-4	45.0	45
ACGTATC	25	3.8920236E-5	45.0	23
CGTTTTT	21070	0.0	43.62245	1
ACGTAGG	120	0.0	43.124996	1
CGACGGT	685	0.0	43.029198	27
TACGCGG	235	0.0	42.12766	1
CACGACG	715	0.0	41.53846	25
GACGGTC	715	0.0	41.223778	28
CGGTCTA	710	0.0	41.197186	30
TAGTCAG	1055	0.0	40.734596	33
CATATGC	2560	0.0	40.60547	32
ACGGGTA	300	0.0	40.500004	4
TAGCATA	2565	0.0	40.438595	29
GCATAGG	490	0.0	40.408165	1
CTTACTG	5800	0.0	40.344826	38
TTACGGG	910	0.0	40.302197	2
GCGCGAC	3110	0.0	40.152733	8
GGTACCT	2650	0.0	40.075474	7
ACTTACT	5880	0.0	40.02551	37
>>END_MODULE