##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043404_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	575049
Sequences flagged as poor quality	0
Sequence length	51
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.589741048154156	31.0	31.0	34.0	30.0	34.0
2	30.59464671706237	31.0	31.0	34.0	26.0	34.0
3	31.487323688937813	31.0	31.0	34.0	28.0	34.0
4	35.110021928566084	37.0	35.0	37.0	32.0	37.0
5	35.41965119494165	37.0	35.0	37.0	33.0	37.0
6	35.84171609723693	37.0	35.0	37.0	35.0	37.0
7	33.72148982086744	37.0	35.0	37.0	32.0	37.0
8	34.796476474178725	37.0	35.0	37.0	31.0	37.0
9	36.84461845860092	39.0	37.0	39.0	32.0	39.0
10	36.78493137106577	39.0	35.0	39.0	32.0	39.0
11	36.314432335331425	38.0	35.0	39.0	32.0	39.0
12	36.06792464642144	38.0	35.0	39.0	32.0	39.0
13	36.14105232771468	39.0	35.0	39.0	32.0	39.0
14	37.519606155301545	40.0	35.0	41.0	33.0	41.0
15	37.719025683028754	40.0	35.0	41.0	33.0	41.0
16	37.685642440905035	40.0	35.0	41.0	33.0	41.0
17	37.68802832454278	39.0	36.0	41.0	33.0	41.0
18	37.38798085032754	39.0	36.0	40.0	33.0	41.0
19	37.06689516893343	37.0	35.0	40.0	33.0	41.0
20	36.72261668136107	37.0	35.0	40.0	33.0	41.0
21	36.63570234884332	37.0	35.0	40.0	32.0	41.0
22	36.550638293432385	37.0	35.0	40.0	32.0	41.0
23	36.46153632125262	36.0	35.0	40.0	32.0	41.0
24	36.31174212980111	36.0	35.0	40.0	32.0	41.0
25	36.21027251590734	35.0	35.0	40.0	32.0	41.0
26	35.94187277953705	35.0	35.0	40.0	31.0	41.0
27	35.88489676531913	36.0	35.0	40.0	31.0	41.0
28	35.81223339228483	36.0	35.0	40.0	31.0	41.0
29	35.75555474403051	36.0	35.0	40.0	31.0	41.0
30	35.58474843013378	35.0	35.0	40.0	31.0	41.0
31	35.12087143878174	35.0	34.0	40.0	30.0	41.0
32	34.98066599541952	35.0	34.0	40.0	29.0	41.0
33	34.84726866753964	35.0	34.0	40.0	27.0	41.0
34	34.65647796970345	35.0	34.0	40.0	26.0	41.0
35	34.41731400280672	35.0	34.0	40.0	24.0	41.0
36	34.234783470625985	35.0	34.0	40.0	23.0	41.0
37	34.154273809710126	35.0	34.0	40.0	23.0	41.0
38	34.07755860804905	35.0	34.0	40.0	23.0	41.0
39	33.86104836283517	35.0	33.0	40.0	22.0	41.0
40	33.852763851428314	35.0	34.0	40.0	22.0	41.0
41	33.8044966602846	35.0	33.0	40.0	22.0	41.0
42	33.70738493589242	35.0	33.0	40.0	21.0	41.0
43	33.531799898791235	35.0	33.0	40.0	21.0	41.0
44	33.332667303134166	35.0	33.0	40.0	20.0	41.0
45	33.168665626755285	35.0	33.0	40.0	20.0	41.0
46	33.182608786381685	35.0	33.0	39.0	20.0	41.0
47	33.18643976426357	35.0	33.0	39.0	20.0	41.0
48	33.128804675775456	35.0	33.0	39.0	20.0	41.0
49	33.00227458877417	35.0	33.0	39.0	18.0	41.0
50	32.87876511392942	35.0	33.0	39.0	18.0	41.0
51	32.4280765639102	35.0	32.0	38.0	17.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	9.0
14	7.0
15	32.0
16	69.0
17	111.0
18	276.0
19	597.0
20	988.0
21	1559.0
22	2534.0
23	3826.0
24	6366.0
25	9562.0
26	11662.0
27	12838.0
28	13088.0
29	13298.0
30	15111.0
31	18114.0
32	22843.0
33	30655.0
34	57884.0
35	86785.0
36	65269.0
37	47807.0
38	58425.0
39	95279.0
40	54.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.15403035219607	17.866999160071575	24.406441885821902	32.57252860191044
2	32.31881109261993	20.475472524950046	28.78137341339608	18.424342969033944
3	21.640242831480446	21.305836546103027	38.886947025383925	18.1669735970326
4	23.07959843422039	24.856490490375602	35.34046663849516	16.723444436908856
5	18.727273675808494	30.781202993136237	33.79503311891682	16.696490212138443
6	77.06369370262361	2.2660677611820907	19.269662237478894	1.4005762987154138
7	73.1565483984843	7.6737808430238115	17.33869635457152	1.8309744039203615
8	73.13741959380853	3.3932760512582405	20.91560893071721	2.5536954242160235
9	42.21448954784723	23.115769264880036	25.892228314456684	8.777512872816056
10	33.724778236289424	20.58398501692899	35.51210418590416	10.179132560877422
11	31.62269650064603	20.00351274413137	35.71486951546738	12.658921239755221
12	16.587629923710846	36.24925875881881	34.65287305951319	12.510238257957148
13	11.70022032905022	38.61583969366089	35.93259009232257	13.751349884966324
14	9.718476164639883	24.61703263547976	51.4931771031686	14.17131409671176
15	10.69769706581526	21.681630608869852	50.717243226229414	16.903429099085468
16	12.802387274823538	21.64563367643453	37.38394467254095	28.168034376200985
17	15.042544200581167	23.728064912729177	43.15075758761427	18.078633299075385
18	19.40425946310662	25.389140751483787	37.1596159631614	18.04698382224819
19	21.207410151134948	25.15246526817715	36.419505120433215	17.220619460254692
20	14.67370606678735	30.277245939041713	38.12283822769886	16.926209766472073
21	16.910906722731454	26.07256077308195	34.523144984166564	22.493387520020033
22	12.467807091221792	30.085262299386663	35.082053877147864	22.364876732243687
23	11.369987601056605	25.061168700406398	48.50769238795303	15.061151310583968
24	12.430766769440517	26.10316686056319	44.885740171707106	16.58032619828919
25	11.894290747397179	32.63721874135943	37.59923067425559	17.869259836987805
26	11.298515430858934	35.45697844879306	36.60974977784502	16.63475634250299
27	11.894116849172853	30.37167267485032	43.05389627666512	14.680314199311711
28	11.242346304401886	25.50704374757629	41.86269344003728	21.387916507984535
29	13.073842402995222	31.28915970639024	39.28447836619141	16.352519524423137
30	20.480863369904128	29.624258106700474	34.6626113600754	15.232267163319996
31	20.452691857563444	27.590518373216895	36.66052805934798	15.29626170987168
32	20.352700378576436	29.661820123154726	33.73955958535708	16.24591991291177
33	14.87142834784514	29.99448742628889	35.51958180955015	19.614502416315826
34	14.419814659272514	28.467834914937683	37.49541343433342	19.61693699145638
35	22.884832422976128	28.72555208338768	32.45532119871524	15.934294294920957
36	16.35860596227452	33.59626744851308	34.93771835095792	15.10740823825448
37	16.07602134774602	33.642872172632245	33.07126870927521	17.209837770346528
38	16.098628116908298	33.47540818260705	33.77607821246537	16.64988548801928
39	19.661976631556616	28.16786047797666	32.69043159800295	19.47973129246377
40	14.413902119645455	27.04534743995729	35.43158930804158	23.109161132355677
41	16.420165933685652	27.889797217280616	33.082745992080675	22.60729085695306
42	16.823783712344515	27.096125721460258	35.15300435267255	20.927086213522674
43	16.090802696813665	29.512789344907997	35.556274334882765	18.840133623395573
44	12.643792094238925	37.88303257635437	31.065004895235017	18.408170434171698
45	17.233661827079082	33.215082540792174	32.69703973052731	16.854215901601428
46	16.890386732261074	30.948145288488462	32.22351486568971	19.93795311356076
47	16.223487041973815	28.807979841717835	36.22856487012412	18.739968246184237
48	17.949948613074714	26.602776459049576	36.43880782333331	19.008467104542394
49	17.264963507457626	31.85745910348509	33.07961582404282	17.797961565014457
50	14.86777648513431	31.433669130804503	32.96936434982062	20.72919003424056
51	14.77874059427979	28.031350371881352	36.67061415635885	20.519294877480007
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	781.0
1	3925.0
2	7069.0
3	27484.5
4	47900.0
5	27477.5
6	7055.0
7	7115.5
8	7176.0
9	8688.0
10	10200.0
11	11338.0
12	12476.0
13	12405.5
14	12335.0
15	11030.0
16	9725.0
17	8319.0
18	6913.0
19	6125.5
20	5338.0
21	5126.0
22	4914.0
23	4495.5
24	4077.0
25	4222.5
26	4609.0
27	4850.0
28	5091.0
29	5332.0
30	6826.5
31	8321.0
32	9876.5
33	11432.0
34	12050.5
35	12669.0
36	13946.0
37	15223.0
38	17700.5
39	20178.0
40	25043.0
41	29908.0
42	36359.0
43	42810.0
44	48753.0
45	54696.0
46	55450.0
47	56204.0
48	49671.5
49	43139.0
50	36560.5
51	29982.0
52	26802.0
53	23622.0
54	21966.5
55	20311.0
56	17042.0
57	13773.0
58	12574.5
59	11376.0
60	9870.0
61	8364.0
62	7035.0
63	5706.0
64	4850.0
65	3994.0
66	3503.5
67	3013.0
68	2709.0
69	2405.0
70	2141.0
71	1877.0
72	1965.0
73	2053.0
74	1771.5
75	1251.0
76	1012.0
77	780.5
78	549.0
79	407.0
80	265.0
81	179.5
82	94.0
83	73.5
84	53.0
85	31.5
86	10.0
87	8.0
88	6.0
89	3.5
90	1.0
91	1.0
92	1.0
93	1.5
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	575049.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.75049251334313
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.49349928915142	26.49194260104609
2	9.35447248126299	6.3143610689028185
3	3.519088066985892	3.563128663758075
4	1.8777957292113205	2.5350612280133755
5	1.2354098664350035	2.0847845724012415
6	0.7826041069683203	1.584796443188753
7	0.5847304321408304	1.3814458050605094
8	0.4352000567281084	1.1750573005126823
9	0.300980712205923	0.9142422546570017
>10	2.511858964004941	18.314263516421363
>50	0.671870213314955	15.925182572617464
>100	0.22831048461790976	10.943526612460266
>500	0.0026122481077564042	0.5841790385105596
>1k	0.001044899243102562	1.0380507092398625
>5k	0.0	0.0
>10k+	5.22449621551281E-4	7.149977613209986
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	40549	7.051399098163809	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4183	0.727416272352443	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1704	0.29632257425019437	No Hit
CTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	775	0.13477112385205436	No Hit
GTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATT	726	0.126250110860118	No Hit
CCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC	658	0.11442503160600226	No Hit
CTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGCT	601	0.10451283281946407	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1112948635681481	0.0
2	0.0	0.0	0.0	0.36344728883973365	0.0
3	0.0	0.0	0.0	0.5872543035463065	0.0
4	0.0	0.0	0.0	0.7578484616093585	0.0
5	0.0	0.0	0.0	1.666118887260042	0.0
6	0.0	0.0	0.0	1.9525292627236983	0.0
7	0.0	0.0	0.0	2.7858495536902073	0.0
8	0.0	0.0	0.0	3.682990492984076	0.0
9	0.0	0.0	0.0	4.397886093185103	0.0
10	0.0	0.0	0.0	4.844630631476622	0.0
11	0.0	0.0	0.0	5.3663253044523165	0.0
12	0.0	0.0	0.0	5.8048966262005495	0.0
13	0.0	0.0	0.0	5.994097894266401	0.0
14	0.0	0.0	0.0	6.14695443344828	0.0
15	0.0	0.0	0.0	6.6733443584807555	0.0
16	0.0	0.0	0.0	7.257816290437859	0.0
17	0.0	0.0	0.0	8.114612841688274	0.0
18	0.0	0.0	0.0	8.462757086787386	0.0
19	0.0	0.0	0.0	8.906893151714028	0.0
20	0.0	0.0	0.0	9.31485838598102	0.0
21	3.4779644865046285E-4	0.0	0.0	9.730127345669674	0.0
22	5.216946729756942E-4	0.0	0.0	10.145222407134002	0.0
23	5.216946729756942E-4	0.0	0.0	10.451978874843709	0.0
24	0.004869150281106479	0.0	0.0	10.723781799464046	0.0
25	0.004869150281106479	0.0	0.0	10.985498627073518	0.0
26	0.004869150281106479	0.0	0.0	11.285299165810217	0.0
27	0.004869150281106479	0.0	0.0	11.539364471549382	0.0
28	0.005043048505431711	0.0	0.0	11.825948745237362	0.0
29	0.005043048505431711	0.0	0.0	12.184700782020315	0.0
30	0.0052169467297569425	0.0	0.0	12.504499616554416	0.0
31	0.0052169467297569425	0.0	0.0	12.769520510426068	0.0
32	0.0052169467297569425	0.0	0.0	13.054713598319447	0.0
33	0.0052169467297569425	0.0	0.0	13.339384991539852	0.0
34	0.0052169467297569425	0.0	0.0	13.692050590471421	0.0
35	0.0052169467297569425	0.0	0.0	14.010110442762269	0.0
36	0.0052169467297569425	0.0	0.0	14.304520136544886	0.0
37	0.0052169467297569425	0.0	0.0	14.609363723787016	0.0
38	0.0052169467297569425	0.0	0.0	14.934031708602223	0.0
39	0.0052169467297569425	0.0	0.0	15.255569525379576	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAG	20	7.030344E-4	45.0	2
AGCGTTA	20	7.030344E-4	45.0	44
CTTCGAC	20	7.030344E-4	45.0	31
CGGAACG	20	7.030344E-4	45.0	29
CTGTCGG	20	7.030344E-4	45.0	1
AGATCTA	20	7.030344E-4	45.0	37
AAATCGT	25	3.888366E-5	45.0	11
ACCGGGT	20	7.030344E-4	45.0	3
CGAACCT	20	7.030344E-4	45.0	21
GTCGATA	20	7.030344E-4	45.0	15
GTATTCC	45	3.8380676E-10	45.0	10
AATCAAT	20	7.030344E-4	45.0	45
TGCTTAG	20	7.030344E-4	45.0	12
CGCGGTA	25	3.888366E-5	45.0	37
TGCCAGG	20	7.030344E-4	45.0	1
CGCGGGT	50	2.1827873E-11	45.0	3
ACGTTGG	50	2.1827873E-11	45.0	1
CGCGGAA	20	7.030344E-4	45.0	27
CTATCGG	25	3.888366E-5	45.0	1
CGTGCCT	20	7.030344E-4	45.0	35
>>END_MODULE