FastQCFastQC Report
Thu 26 May 2016
SRR1043403_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043403_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1480366
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT717864.84920620981568No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74590.5038618828046577No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29130.19677566223487977No Hit
GCGAAGGGTCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCACAAGCCA16730.11301259283177267No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCGAT302.165927E-645.00000422
ACCGGCG650.045.00000419
CACGGCA302.165927E-645.0000043
ACGGCAG207.0339453E-445.0000044
GTATCGC207.0339453E-445.00000416
GCGTCAT650.045.00000436
ATCGCCT207.0339453E-445.00000416
TCGCGAT207.0339453E-445.00000445
GATCGTC253.8913517E-545.08
CTCCGAT253.8913517E-545.026
TTTCGAT253.8913517E-545.015
GTCACGG2050.045.01
TGTTCGA253.8913517E-545.011
ACGTCCG900.045.039
CGATGTA453.8562575E-1045.09
GCGCTTA253.8913517E-545.045
CGGACTA253.8913517E-545.05
CTAGCGA253.8913517E-545.01
CGTTTTT191950.044.296691
ATTGCGG3650.043.7671241