##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043403_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1480366
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.518368430509753	31.0	31.0	33.0	30.0	34.0
2	30.55271871955989	31.0	31.0	34.0	26.0	34.0
3	31.48140122104939	31.0	31.0	34.0	28.0	34.0
4	35.10075346231945	37.0	35.0	37.0	32.0	37.0
5	35.414821740029154	37.0	35.0	37.0	33.0	37.0
6	35.84064143596921	37.0	35.0	37.0	35.0	37.0
7	33.788242231988576	37.0	35.0	37.0	32.0	37.0
8	34.79485546141968	37.0	35.0	37.0	32.0	37.0
9	36.737272404256785	39.0	37.0	39.0	32.0	39.0
10	36.643974530622835	39.0	35.0	39.0	32.0	39.0
11	36.51164982173327	39.0	35.0	39.0	32.0	39.0
12	36.49110827997941	39.0	35.0	39.0	32.0	39.0
13	36.53917342062706	39.0	35.0	39.0	32.0	39.0
14	37.89391001954922	40.0	36.0	41.0	33.0	41.0
15	38.02727703824595	40.0	36.0	41.0	33.0	41.0
16	37.99308144067075	40.0	36.0	41.0	33.0	41.0
17	37.92350202585036	40.0	36.0	41.0	33.0	41.0
18	37.65247107809825	39.0	36.0	41.0	33.0	41.0
19	37.35994139287176	38.0	36.0	41.0	33.0	41.0
20	37.06168001696878	38.0	35.0	41.0	33.0	41.0
21	36.96508431023139	38.0	35.0	41.0	32.0	41.0
22	36.87191883628778	38.0	35.0	40.0	32.0	41.0
23	36.79332948743757	38.0	35.0	40.0	32.0	41.0
24	36.642689037710944	38.0	35.0	40.0	32.0	41.0
25	36.54192274072763	38.0	35.0	40.0	32.0	41.0
26	36.29098547251153	38.0	35.0	40.0	31.0	41.0
27	36.20297750691383	37.0	35.0	40.0	31.0	41.0
28	36.09729012960308	37.0	35.0	40.0	31.0	41.0
29	36.01873658270995	37.0	35.0	40.0	31.0	41.0
30	35.82514256609514	37.0	35.0	40.0	30.0	41.0
31	35.41100038774195	37.0	35.0	40.0	29.0	41.0
32	35.21343302939949	37.0	34.0	40.0	26.0	41.0
33	35.03014997642475	37.0	34.0	40.0	25.0	41.0
34	34.79922600221837	37.0	34.0	40.0	23.0	41.0
35	34.55588415297298	37.0	34.0	40.0	22.0	41.0
36	34.386146398931075	37.0	34.0	40.0	21.0	41.0
37	34.2746509984693	37.0	34.0	40.0	21.0	41.0
38	34.157486054124455	37.0	34.0	40.0	20.0	41.0
39	34.03864247084842	37.0	34.0	40.0	20.0	41.0
40	33.96096033008053	37.0	33.0	40.0	18.0	41.0
41	33.8838766899537	36.0	33.0	40.0	18.0	41.0
42	33.797186641681854	36.0	33.0	40.0	18.0	41.0
43	33.64180344590459	36.0	33.0	40.0	18.0	41.0
44	33.45003330257517	36.0	33.0	40.0	18.0	41.0
45	33.26486828257336	35.0	33.0	40.0	17.0	41.0
46	33.270747909638565	35.0	33.0	40.0	17.0	41.0
47	33.24589729837081	35.0	33.0	40.0	17.0	41.0
48	33.16429045249621	35.0	33.0	40.0	17.0	41.0
49	33.08475944462383	35.0	33.0	40.0	15.0	41.0
50	32.98059871680382	35.0	33.0	40.0	15.0	41.0
51	32.53137805110358	35.0	32.0	39.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	3.0
12	6.0
13	18.0
14	32.0
15	76.0
16	179.0
17	392.0
18	890.0
19	1617.0
20	2809.0
21	4570.0
22	7006.0
23	11062.0
24	17912.0
25	27062.0
26	34415.0
27	36955.0
28	36198.0
29	36265.0
30	39184.0
31	45382.0
32	55150.0
33	71093.0
34	114340.0
35	155921.0
36	151704.0
37	143288.0
38	189184.0
39	297521.0
40	131.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.127322567527216	18.40119267802692	25.384871038648548	30.086613715797313
2	32.27796369276247	20.452509717191557	28.649469117772224	18.62005747227375
3	21.333102759722934	21.819739172610017	38.368011694405304	18.47914637326175
4	22.085078960203084	24.702877531637448	35.2152102925898	17.996833215569662
5	19.47707526381989	31.112576214260528	33.18726585182313	16.22308267009645
6	78.35359634036448	2.0366585020190953	18.22724920729063	1.3824959503257979
7	74.9033009404431	7.173023427990105	16.4995007991267	1.4241748324400858
8	74.11856257168836	3.9813127294196162	19.298470783576494	2.601653915315537
9	37.6504864337603	25.352784378998166	26.166366966007054	10.830362221234479
10	26.35814386442272	24.7076736428694	37.370893414196225	11.563289078511666
11	25.66041100646732	21.68051684515856	38.88788313160394	13.771189016770178
12	20.17555118126193	26.56768663965533	36.961737840507006	16.295024338575733
13	15.331613938715154	29.177379107599066	37.43884958179261	18.05215737189317
14	14.65786163691952	24.434025099198443	43.09589655531132	17.81221670857072
15	16.855493844089906	22.969589952754927	42.507055687579964	17.6678605155752
16	18.520757704513613	23.56268652481886	36.868720302952106	21.047835467715416
17	18.786908102455744	23.305115086404307	40.558618611883816	17.34935819925613
18	21.72280368503465	25.017326796211208	36.14943871988414	17.11043079887001
19	21.658360162284193	25.329074026288094	36.59912481102646	16.413441000401253
20	18.578175937572194	27.303180429704543	37.19958442709438	16.919059205628876
21	18.74603983069052	24.5973630845345	36.647964084557465	20.008633000217515
22	15.455772423846536	27.085464000118893	37.97506832769734	19.483695248337234
23	15.501031501669182	24.929375573337946	43.236469900011215	16.33312302498166
24	16.25652034699527	25.615422132094363	40.69385543845238	17.434202082457986
25	16.188023772499506	29.13286308926306	36.99389205101981	17.68522108721762
26	15.279194469475794	28.981414055713252	37.666158233842175	18.073233240968786
27	16.210585760548405	26.95623920030587	39.76678740257477	17.06638763657096
28	15.092416334879347	25.239839337028812	39.95282247768458	19.714921850407265
29	16.579683672821453	28.73924421393088	38.53161988319105	16.14945223005662
30	19.190389403701516	28.028474039528064	36.51759092008328	16.263545636687144
31	19.605219249834164	27.269675201943304	36.22867588150498	16.896429666717555
32	18.563584951289073	28.72586914317135	35.436101612709294	17.27444429283029
33	16.877920730413965	28.25713370882606	36.3158840448916	18.549061515868374
34	17.640705068881616	27.721387818958288	36.196589221854595	18.441317890305506
35	20.294238046537142	27.85412526361724	34.74296221339858	17.108674476447042
36	17.99744117333146	30.029668338775682	34.73485610990796	17.238034377984903
37	17.805934478365483	29.057881631974798	35.472443976692254	17.66373991296747
38	17.957383511915296	31.027597229333825	33.59088225479375	17.424137003957128
39	20.351656279595723	28.228897448333722	32.90517345034944	18.514272821721114
40	16.764232628957974	27.91755552343137	35.38584377106743	19.932368076543234
41	18.139838391316744	28.523351657630613	32.5326979949553	20.804111956097344
42	17.481285033566024	28.00469613595557	34.09656801088379	20.417450819594613
43	17.66637439660192	28.892787324215767	34.036515294190764	19.40432298499155
44	16.024753338025867	32.781893126429544	32.51256783795359	18.680785697591002
45	18.32925100954764	30.575817061456423	33.17395833192602	17.920973597069914
46	17.376851400261828	29.827623709271894	33.326015323237634	19.469509567228645
47	16.94378282127528	28.890558145755847	35.4654862378628	18.700172795106077
48	18.21056414427243	27.86135320589638	34.37082451231655	19.55725813751464
49	18.33364181560506	29.67279713260099	33.51326631387103	18.480294737922918
50	17.04402830110932	28.720465074177604	33.96410076967452	20.27140585503855
51	16.880014807149042	28.02185405501072	34.96169190592056	20.136439231919674
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1034.0
1	7492.5
2	13951.0
3	54477.5
4	95004.0
5	57965.5
6	20927.0
7	20378.0
8	19829.0
9	21875.0
10	23921.0
11	25319.5
12	26718.0
13	26180.0
14	25642.0
15	23665.0
16	21688.0
17	19332.5
18	16977.0
19	15050.5
20	13124.0
21	12923.5
22	12723.0
23	12798.5
24	12874.0
25	12699.5
26	13314.0
27	14103.0
28	16884.5
29	19666.0
30	22161.5
31	24657.0
32	27710.0
33	30763.0
34	37061.0
35	43359.0
36	47674.0
37	51989.0
38	56278.0
39	60567.0
40	66858.5
41	73150.0
42	82751.0
43	92352.0
44	97836.0
45	103320.0
46	105750.5
47	108181.0
48	108256.5
49	108332.0
50	104703.0
51	101074.0
52	89261.5
53	77449.0
54	70511.0
55	63573.0
56	57472.0
57	51371.0
58	47324.5
59	43278.0
60	34782.5
61	26287.0
62	22102.0
63	17917.0
64	15831.5
65	13746.0
66	11888.5
67	10031.0
68	8127.5
69	6224.0
70	6062.0
71	5900.0
72	6059.5
73	6219.0
74	5332.0
75	3645.0
76	2845.0
77	2163.0
78	1481.0
79	1088.0
80	695.0
81	466.0
82	237.0
83	182.0
84	127.0
85	93.5
86	60.0
87	34.5
88	9.0
89	7.0
90	5.0
91	4.5
92	4.0
93	5.5
94	7.0
95	6.5
96	6.0
97	3.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1480366.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.57584362845717
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.24919650430623	26.27282785881004
2	9.390602733152033	6.3059481789055045
3	3.402740633658537	3.4274966227174892
4	1.7870347114724496	2.400047921240962
5	1.119926668608897	1.8801241350275653
6	0.7558139889633785	1.522625538338091
7	0.5764029359274685	1.354725039057901
8	0.4197320216571958	1.1274285380014566
9	0.3425835804180676	1.0352279453214617
>10	3.1169359822470053	23.156017892001472
>50	0.6419001263779057	15.003459082793718
>100	0.1912456325083055	9.38396220901193
>500	0.004413360526852942	0.9556347278674611
>1k	0.0010508001254411767	0.5827092655046514
>5k	2.1016002508823534E-4	0.5263294273915228
>10k+	2.1016002508823534E-4	5.065435618008828
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	71786	4.84920620981568	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7459	0.5038618828046577	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2913	0.19677566223487977	No Hit
GCGAAGGGTCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCACAAGCCA	1673	0.11301259283177267	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.07106350726779728	0.0
2	0.0	0.0	0.0	0.23082129689549746	0.0
3	0.0	0.0	0.0	0.3745695321292167	0.0
4	0.0	0.0	0.0	0.474679910238414	0.0
5	0.0	0.0	0.0	1.0386620605985277	0.0
6	0.0	0.0	0.0	1.2044318769817735	0.0
7	0.0	0.0	0.0	1.671613641491361	0.0
8	0.0	0.0	0.0	2.212155642591089	0.0
9	0.0	0.0	0.0	2.639955254308732	0.0
10	0.0	0.0	0.0	2.9597410370138197	0.0
11	0.0	0.0	0.0	3.3056014526137454	0.0
12	0.0	0.0	0.0	3.59985300932337	0.0
13	0.0	0.0	0.0	3.7303612755224047	0.0
14	0.0	0.0	0.0	3.8333087898533202	0.0
15	0.0	0.0	0.0	4.177818188204808	0.0
16	0.0	0.0	0.0	4.599538222304484	0.0
17	0.0	0.0	0.0	5.101913986135861	0.0
18	0.0	0.0	0.0	5.388397193666972	0.0
19	0.0	0.0	0.0	5.693524439226516	0.0
20	0.0	0.0	0.0	6.058704401479094	0.0
21	1.351017248437211E-4	0.0	0.0	6.4490808354150255	0.0
22	2.0265258726558162E-4	0.0	0.0	6.843105015921738	0.0
23	2.0265258726558162E-4	0.0	0.0	7.1728207754028395	0.0
24	6.755086242186054E-4	0.0	0.0	7.466329272625823	0.0
25	7.430594866404659E-4	0.0	0.0	7.750515750834591	0.0
26	7.430594866404659E-4	0.0	0.0	8.07070683871421	0.0
27	9.457120739060475E-4	0.0	0.0	8.385021001563127	0.0
28	0.001013262936327908	0.0	0.0	8.706360454103917	0.0
29	0.001013262936327908	0.0	0.0	9.092548734569695	0.0
30	0.001013262936327908	0.0	0.0	9.45259483127821	0.0
31	0.001013262936327908	6.755086242186054E-5	0.0	9.773934283819	0.0
32	0.001013262936327908	6.755086242186054E-5	0.0	10.127968353771973	0.0
33	0.001013262936327908	6.755086242186054E-5	0.0	10.48004344871471	0.0
34	0.001013262936327908	6.755086242186054E-5	0.0	10.890752692239621	0.0
35	0.001013262936327908	6.755086242186054E-5	0.0	11.260796316586573	0.0
36	0.001013262936327908	6.755086242186054E-5	0.0	11.644687867730008	0.0
37	0.001013262936327908	6.755086242186054E-5	0.0	12.041211430146328	0.0
38	0.001013262936327908	6.755086242186054E-5	0.0	12.45009680038585	0.0
39	0.001013262936327908	6.755086242186054E-5	0.0	12.855672178366701	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGCGAT	30	2.165927E-6	45.000004	22
ACCGGCG	65	0.0	45.000004	19
CACGGCA	30	2.165927E-6	45.000004	3
ACGGCAG	20	7.0339453E-4	45.000004	4
GTATCGC	20	7.0339453E-4	45.000004	16
GCGTCAT	65	0.0	45.000004	36
ATCGCCT	20	7.0339453E-4	45.000004	16
TCGCGAT	20	7.0339453E-4	45.000004	45
GATCGTC	25	3.8913517E-5	45.0	8
CTCCGAT	25	3.8913517E-5	45.0	26
TTTCGAT	25	3.8913517E-5	45.0	15
GTCACGG	205	0.0	45.0	1
TGTTCGA	25	3.8913517E-5	45.0	11
ACGTCCG	90	0.0	45.0	39
CGATGTA	45	3.8562575E-10	45.0	9
GCGCTTA	25	3.8913517E-5	45.0	45
CGGACTA	25	3.8913517E-5	45.0	5
CTAGCGA	25	3.8913517E-5	45.0	1
CGTTTTT	19195	0.0	44.29669	1
ATTGCGG	365	0.0	43.767124	1
>>END_MODULE