FastQCFastQC Report
Thu 26 May 2016
SRR1043402_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043402_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1258576
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT792126.293779636668743No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80070.6361951920265443No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32950.26180381637660344No Hit
GTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATT14350.11401774704110042No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCGG453.8562575E-1045.0000041
CTGTCGG406.8157533E-945.01
CGTGCTA207.0335425E-445.044
CGCATTA207.0335425E-445.014
TAGGTCG207.0335425E-445.041
CGCGATA207.0335425E-445.010
CAGATCG207.0335425E-445.017
TGTTACG207.0335425E-445.014
AGCGATA207.0335425E-445.07
CGGTCTA1450.045.030
CGATCGA207.0335425E-445.09
GCGTCAA207.0335425E-445.022
CCGCATT207.0335425E-445.013
GCCGTGA207.0335425E-445.031
CCCGCAT207.0335425E-445.012
CTTCGTA302.165667E-644.99999619
CAGCGCA253.891019E-544.99999611
CGGTTAG253.891019E-544.99999630
TCGATAT253.891019E-544.99999624
CGTTTTT175400.044.409921