##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043402_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1258576
Sequences flagged as poor quality	0
Sequence length	51
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.521223191924843	31.0	31.0	33.0	30.0	34.0
2	30.54091052109686	31.0	31.0	34.0	26.0	34.0
3	31.463890142510266	31.0	31.0	34.0	28.0	34.0
4	35.08905223045728	37.0	35.0	37.0	32.0	37.0
5	35.41856749214986	37.0	35.0	37.0	33.0	37.0
6	35.832214343829854	37.0	35.0	37.0	35.0	37.0
7	33.73136942067861	37.0	35.0	37.0	32.0	37.0
8	34.76565737786196	37.0	35.0	37.0	31.0	37.0
9	36.75514231957387	39.0	37.0	39.0	32.0	39.0
10	36.67805917163525	39.0	35.0	39.0	32.0	39.0
11	36.59693733235021	39.0	35.0	39.0	32.0	39.0
12	36.59578285300212	39.0	35.0	39.0	32.0	39.0
13	36.650559044507446	39.0	35.0	39.0	32.0	39.0
14	38.011776007170006	40.0	36.0	41.0	33.0	41.0
15	38.10906691371836	40.0	37.0	41.0	33.0	41.0
16	38.054011835598324	40.0	36.0	41.0	33.0	41.0
17	38.010894852595314	40.0	36.0	41.0	33.0	41.0
18	37.722286933804554	39.0	36.0	41.0	33.0	41.0
19	37.43407231664993	38.0	36.0	41.0	33.0	41.0
20	37.11579753626321	38.0	35.0	41.0	33.0	41.0
21	37.00240986638868	38.0	35.0	41.0	33.0	41.0
22	36.90170478381917	38.0	35.0	40.0	32.0	41.0
23	36.812577865778465	38.0	35.0	40.0	32.0	41.0
24	36.63641528203303	38.0	35.0	40.0	32.0	41.0
25	36.52340899556324	38.0	35.0	40.0	32.0	41.0
26	36.23954930016145	37.0	35.0	40.0	31.0	41.0
27	36.13428191861278	37.0	35.0	40.0	31.0	41.0
28	36.02244044062496	37.0	35.0	40.0	31.0	41.0
29	35.95007691231996	37.0	35.0	40.0	31.0	41.0
30	35.7749837912053	37.0	35.0	40.0	30.0	41.0
31	35.304866770063946	37.0	35.0	40.0	29.0	41.0
32	35.117933283329734	37.0	34.0	40.0	26.0	41.0
33	34.95099699978388	37.0	34.0	40.0	25.0	41.0
34	34.7199557277431	37.0	34.0	40.0	23.0	41.0
35	34.51370437701021	37.0	34.0	40.0	22.0	41.0
36	34.34189909866389	37.0	34.0	40.0	22.0	41.0
37	34.25329896645097	36.0	34.0	40.0	21.0	41.0
38	34.142689039040945	36.0	34.0	40.0	21.0	41.0
39	34.02972645275295	36.0	34.0	40.0	20.0	41.0
40	33.946624597958326	36.0	33.0	40.0	20.0	41.0
41	33.85955476665692	36.0	33.0	40.0	20.0	41.0
42	33.765166346728364	35.0	33.0	40.0	19.0	41.0
43	33.62061409084553	35.0	33.0	40.0	18.0	41.0
44	33.437453916171926	35.0	33.0	40.0	18.0	41.0
45	33.2492674260434	35.0	33.0	40.0	18.0	41.0
46	33.25157717928834	35.0	33.0	40.0	18.0	41.0
47	33.24365632270121	35.0	33.0	40.0	18.0	41.0
48	33.16781982176682	35.0	33.0	40.0	18.0	41.0
49	33.09754992944407	35.0	33.0	40.0	17.0	41.0
50	32.98584988113551	35.0	33.0	40.0	17.0	41.0
51	32.607467487064746	35.0	32.0	39.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	6.0
12	7.0
13	18.0
14	27.0
15	65.0
16	168.0
17	378.0
18	762.0
19	1386.0
20	2308.0
21	3818.0
22	5902.0
23	9180.0
24	14687.0
25	22293.0
26	27992.0
27	30688.0
28	30482.0
29	30764.0
30	33121.0
31	38617.0
32	46447.0
33	59770.0
34	97721.0
35	135874.0
36	146668.0
37	119569.0
38	154903.0
39	244845.0
40	107.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.587489353046617	18.547946250365495	24.4624877639491	31.402076632638792
2	33.139357496090824	20.197429475852076	27.8680032036206	18.79520982443651
3	21.434382985215038	20.919674298572353	39.398494806829305	18.2474479093833
4	21.334349296347618	24.683769593572418	36.370628392723205	17.61125271735676
5	19.683435883093274	30.86829877575927	33.85985431153939	15.588411029608066
6	77.45428166435718	1.9185968904539732	19.39199539797358	1.2351260472152654
7	73.74930079709131	7.1534813948462395	17.813306466991268	1.2839113410711789
8	72.79838484128094	4.24662475686808	20.552036587381295	2.4029538144696865
9	35.686124636096665	28.058138721857084	26.468246653360623	9.787489988685625
10	24.539082264400403	23.78537331078934	39.85472470474568	11.82081972006458
11	23.756133916426183	22.277240309683325	40.26026239178246	13.706363382108034
12	18.577662373984566	27.382692821093045	38.98111834327049	15.0585264616519
13	14.245623625430644	29.895453274176532	39.09736082683922	16.761562273553604
14	13.990017289381015	25.52551454977689	43.986060436556876	16.498407724285226
15	16.722629384320058	24.719524287766493	42.499618616595264	16.05822771131819
16	17.230266586999914	25.06340499103749	38.11434510113016	19.591983320832433
17	17.35127636312785	25.43334689363217	40.921247505116895	16.294129238123084
18	19.32938495569596	27.11540661827335	37.58199743201841	15.97321099401228
19	20.091675035913603	26.845895678926023	37.87002135747067	15.192407927689707
20	17.200232643876888	29.495795247962775	37.61965904323616	15.684313064924169
21	17.50287626651072	26.639392456236255	37.488320133229934	18.369411144023086
22	14.726802354406884	28.544482017772467	38.41508180674032	18.313633821080334
23	14.662364449981565	26.414296792565565	43.87657161744702	15.046767140005848
24	15.377776153366979	27.91591449384066	40.55527834632156	16.151031006470806
25	15.4035989880627	30.382988393231713	37.68910260484866	16.52431001385693
26	14.131367513761584	30.467766745909664	38.04378917125386	17.35707656907489
27	14.75953776331346	28.841404889335248	40.06305538958315	16.336001957768147
28	13.789234817762297	26.911287041863186	40.046846594881835	19.252631545492687
29	15.031988533072296	30.32792616417284	38.91874626562083	15.72133903713403
30	18.30950216752902	29.704364297428203	35.861322637647625	16.12481089739515
31	18.062953687341885	29.07651186738028	36.30054919210282	16.559985253175018
32	17.084625799316054	30.619763923672465	35.36115419331053	16.934456083700944
33	15.808659945843557	29.412447083052594	35.99464791955353	18.784245051550325
34	15.47653856421861	30.01177521262125	36.392716848247545	18.1189693749126
35	18.722826432412504	31.1522705025362	33.64667687926673	16.47822618578457
36	16.05449333214681	32.692423818664906	34.91342596712475	16.33965688206354
37	16.12187106698364	32.17970150392189	35.13399270286419	16.564434726230278
38	15.88525444629486	34.04283889093706	33.54640482577135	16.525501836996735
39	18.108640241034312	31.030148358144444	33.62252259696673	17.238688803854515
40	15.38452981782586	30.506699635143207	35.36846404190132	18.740306505129606
41	15.970350618476756	31.056924651352002	32.87858659310204	20.094138137069194
42	15.805005021548164	30.23877779331562	34.250692846518604	19.70552433861761
43	16.190837899340206	31.080522749520096	34.061908061173895	18.666731289965803
44	14.934497400236458	34.08431433620218	32.9229224139027	18.05826584965866
45	17.03655559934402	32.482186216803754	33.07348940389774	17.407768779954488
46	15.594052325803128	32.531607149667565	33.201809028616466	18.672531495912843
47	15.434824754325524	31.015210841458916	35.186432921015495	18.36353148320006
48	16.60328816058784	29.961957005377503	34.828250340066866	18.606504493967783
49	16.761800638181565	31.509261260345024	33.78031998067657	17.948618120796837
50	15.102941737328537	31.154018509807912	34.12253213155185	19.620507621311706
51	15.404870266078488	30.218119525559047	34.808307166194176	19.56870304216829
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1009.0
1	7669.5
2	14330.0
3	57433.5
4	100537.0
5	59502.5
6	18468.0
7	18424.5
8	18381.0
9	21287.0
10	24193.0
11	26997.5
12	29802.0
13	28993.0
14	28184.0
15	25543.5
16	22903.0
17	19941.0
18	16979.0
19	15004.5
20	13030.0
21	12441.0
22	11852.0
23	11197.0
24	10542.0
25	11078.5
26	13375.5
27	15136.0
28	16568.0
29	18000.0
30	21181.5
31	24363.0
32	26916.0
33	29469.0
34	35375.5
35	41282.0
36	43111.0
37	44940.0
38	47257.0
39	49574.0
40	54468.5
41	59363.0
42	65820.5
43	72278.0
44	77019.5
45	81761.0
46	81944.5
47	82128.0
48	82970.5
49	83813.0
50	78815.5
51	73818.0
52	67873.0
53	61928.0
54	56216.5
55	50505.0
56	45728.5
57	40952.0
58	35834.0
59	30716.0
60	27355.0
61	23994.0
62	20114.0
63	16234.0
64	13734.0
65	11234.0
66	9277.0
67	7320.0
68	6075.5
69	4831.0
70	4400.0
71	3969.0
72	3862.5
73	3756.0
74	3172.0
75	2059.5
76	1531.0
77	1126.5
78	722.0
79	516.5
80	311.0
81	217.0
82	123.0
83	91.0
84	59.0
85	39.0
86	19.0
87	21.0
88	23.0
89	12.5
90	2.0
91	4.0
92	6.0
93	4.5
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1258576.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.251436931658056
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.10969456804366	26.75369277248421
2	8.900527688753067	6.097117255796038
3	3.4138639038986565	3.507892325929464
4	1.908510497755966	2.6147690778918316
5	1.2465382631775062	2.134786335206146
6	0.8422311941467846	1.7308577176916184
7	0.6021064134019242	1.4436106891347986
8	0.4528604316126365	1.2408896409778931
9	0.3784022925984448	1.1664740033757404
>10	3.4624575422417743	26.328815096037435
>50	0.5776320850357678	13.270695898023071
>100	0.10154839108993662	5.366911946209362
>500	0.0021760369463673986	0.5310847015194797
>1k	9.67127531718844E-4	0.5894684087658906
>5k	2.41781882929711E-4	0.663089849534769
>10k+	2.41781882929711E-4	6.559844281422271
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	79212	6.293779636668743	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8007	0.6361951920265443	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3295	0.26180381637660344	No Hit
GTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATT	1435	0.11401774704110042	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06674209582893684	0.0
2	0.0	0.0	0.0	0.21842145408779445	0.0
3	0.0	0.0	0.0	0.36056622722823256	0.0
4	0.0	0.0	0.0	0.44868168469762654	0.0
5	0.0	0.0	0.0	0.967760389519584	0.0
6	0.0	0.0	0.0	1.1493147811494895	0.0
7	0.0	0.0	0.0	1.603558307166194	0.0
8	0.0	0.0	0.0	2.106110397782891	0.0
9	0.0	0.0	0.0	2.4967105681341453	0.0
10	0.0	0.0	0.0	2.769240792768971	0.0
11	0.0	0.0	0.0	3.068865130115305	0.0
12	0.0	0.0	0.0	3.3184328955899365	0.0
13	0.0	0.0	0.0	3.4377741193221545	0.0
14	0.0	0.0	0.0	3.5347090680260864	0.0
15	0.0	0.0	0.0	3.8304401164490662	0.0
16	0.0	0.0	0.0	4.2049904018509805	0.0
17	0.0	0.0	0.0	4.628882165240716	0.0
18	0.0	0.0	0.0	4.877734836831467	0.0
19	0.0	0.0	0.0	5.135963183788663	0.0
20	0.0	0.0	0.0	5.431058593203748	0.0
21	0.0	0.0	0.0	5.750626104422776	0.0
22	0.0	0.0	0.0	6.086958594475026	0.0
23	0.0	0.0	0.0	6.355277710682549	0.0
24	2.3836462796048867E-4	0.0	0.0	6.590225778975604	0.0
25	2.3836462796048867E-4	0.0	0.0	6.83669480428675	0.0
26	2.3836462796048867E-4	0.0	0.0	7.097465707275524	0.0
27	2.3836462796048867E-4	0.0	0.0	7.347033472750156	0.0
28	2.3836462796048867E-4	0.0	0.0	7.612412758546166	0.0
29	2.3836462796048867E-4	0.0	0.0	7.919585309111249	0.0
30	2.3836462796048867E-4	0.0	0.0	8.21301216613061	0.0
31	2.3836462796048867E-4	0.0	0.0	8.477358538538793	0.0
32	2.3836462796048867E-4	0.0	0.0	8.755530059368683	0.0
33	2.3836462796048867E-4	0.0	0.0	9.04331562019298	0.0
34	2.3836462796048867E-4	0.0	0.0	9.377026099337664	0.0
35	2.3836462796048867E-4	0.0	0.0	9.66179237487446	0.0
36	2.3836462796048867E-4	0.0	0.0	9.973970582626714	0.0
37	2.3836462796048867E-4	0.0	0.0	10.290518808558243	0.0
38	2.3836462796048867E-4	0.0	0.0	10.606034121101944	0.0
39	2.3836462796048867E-4	0.0	0.0	10.947610632969324	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGTCGG	45	3.8562575E-10	45.000004	1
CTGTCGG	40	6.8157533E-9	45.0	1
CGTGCTA	20	7.0335425E-4	45.0	44
CGCATTA	20	7.0335425E-4	45.0	14
TAGGTCG	20	7.0335425E-4	45.0	41
CGCGATA	20	7.0335425E-4	45.0	10
CAGATCG	20	7.0335425E-4	45.0	17
TGTTACG	20	7.0335425E-4	45.0	14
AGCGATA	20	7.0335425E-4	45.0	7
CGGTCTA	145	0.0	45.0	30
CGATCGA	20	7.0335425E-4	45.0	9
GCGTCAA	20	7.0335425E-4	45.0	22
CCGCATT	20	7.0335425E-4	45.0	13
GCCGTGA	20	7.0335425E-4	45.0	31
CCCGCAT	20	7.0335425E-4	45.0	12
CTTCGTA	30	2.165667E-6	44.999996	19
CAGCGCA	25	3.891019E-5	44.999996	11
CGGTTAG	25	3.891019E-5	44.999996	30
TCGATAT	25	3.891019E-5	44.999996	24
CGTTTTT	17540	0.0	44.40992	1
>>END_MODULE